Job ID = 14171676 SRX = SRX9986115 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:24 19896350 reads; of these: 19896350 (100.00%) were unpaired; of these: 2573871 (12.94%) aligned 0 times 14355877 (72.15%) aligned exactly 1 time 2966602 (14.91%) aligned >1 times 87.06% overall alignment rate Time searching: 00:04:24 Overall time: 00:04:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4032877 / 17322479 = 0.2328 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:32:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:32:43: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:32:43: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:32:48: 1000000 INFO @ Sat, 11 Dec 2021 12:32:54: 2000000 INFO @ Sat, 11 Dec 2021 12:32:59: 3000000 INFO @ Sat, 11 Dec 2021 12:33:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:33:10: 5000000 INFO @ Sat, 11 Dec 2021 12:33:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:33:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:33:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:33:16: 6000000 INFO @ Sat, 11 Dec 2021 12:33:17: 1000000 INFO @ Sat, 11 Dec 2021 12:33:22: 2000000 INFO @ Sat, 11 Dec 2021 12:33:22: 7000000 INFO @ Sat, 11 Dec 2021 12:33:27: 3000000 INFO @ Sat, 11 Dec 2021 12:33:28: 8000000 INFO @ Sat, 11 Dec 2021 12:33:32: 4000000 INFO @ Sat, 11 Dec 2021 12:33:34: 9000000 INFO @ Sat, 11 Dec 2021 12:33:37: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:33:40: 10000000 INFO @ Sat, 11 Dec 2021 12:33:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:33:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:33:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:33:43: 6000000 INFO @ Sat, 11 Dec 2021 12:33:47: 1000000 INFO @ Sat, 11 Dec 2021 12:33:47: 11000000 INFO @ Sat, 11 Dec 2021 12:33:48: 7000000 INFO @ Sat, 11 Dec 2021 12:33:52: 2000000 INFO @ Sat, 11 Dec 2021 12:33:53: 12000000 INFO @ Sat, 11 Dec 2021 12:33:53: 8000000 INFO @ Sat, 11 Dec 2021 12:33:57: 3000000 INFO @ Sat, 11 Dec 2021 12:33:58: 9000000 INFO @ Sat, 11 Dec 2021 12:33:59: 13000000 INFO @ Sat, 11 Dec 2021 12:34:01: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:34:01: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:34:01: #1 total tags in treatment: 13289602 INFO @ Sat, 11 Dec 2021 12:34:01: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:34:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:34:01: #1 tags after filtering in treatment: 13289486 INFO @ Sat, 11 Dec 2021 12:34:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:34:01: #1 finished! INFO @ Sat, 11 Dec 2021 12:34:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:34:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:34:02: #2 number of paired peaks: 54 WARNING @ Sat, 11 Dec 2021 12:34:02: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:34:02: Process for pairing-model is terminated! INFO @ Sat, 11 Dec 2021 12:34:02: 4000000 cut: /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:34:03: 10000000 INFO @ Sat, 11 Dec 2021 12:34:07: 5000000 INFO @ Sat, 11 Dec 2021 12:34:09: 11000000 INFO @ Sat, 11 Dec 2021 12:34:13: 6000000 INFO @ Sat, 11 Dec 2021 12:34:14: 12000000 INFO @ Sat, 11 Dec 2021 12:34:18: 7000000 INFO @ Sat, 11 Dec 2021 12:34:19: 13000000 INFO @ Sat, 11 Dec 2021 12:34:21: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:34:21: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:34:21: #1 total tags in treatment: 13289602 INFO @ Sat, 11 Dec 2021 12:34:21: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:34:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:34:21: #1 tags after filtering in treatment: 13289486 INFO @ Sat, 11 Dec 2021 12:34:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:34:21: #1 finished! INFO @ Sat, 11 Dec 2021 12:34:21: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:34:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:34:22: #2 number of paired peaks: 54 WARNING @ Sat, 11 Dec 2021 12:34:22: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:34:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:34:23: 8000000 INFO @ Sat, 11 Dec 2021 12:34:28: 9000000 INFO @ Sat, 11 Dec 2021 12:34:33: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:34:38: 11000000 INFO @ Sat, 11 Dec 2021 12:34:43: 12000000 INFO @ Sat, 11 Dec 2021 12:34:48: 13000000 INFO @ Sat, 11 Dec 2021 12:34:49: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:34:49: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:34:49: #1 total tags in treatment: 13289602 INFO @ Sat, 11 Dec 2021 12:34:49: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:34:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:34:50: #1 tags after filtering in treatment: 13289486 INFO @ Sat, 11 Dec 2021 12:34:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:34:50: #1 finished! INFO @ Sat, 11 Dec 2021 12:34:50: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:34:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:34:51: #2 number of paired peaks: 54 WARNING @ Sat, 11 Dec 2021 12:34:51: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:34:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986115/SRX9986115.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。