Job ID = 14171663 SRX = SRX9986113 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:43 17487310 reads; of these: 17487310 (100.00%) were unpaired; of these: 2188048 (12.51%) aligned 0 times 12242258 (70.01%) aligned exactly 1 time 3057004 (17.48%) aligned >1 times 87.49% overall alignment rate Time searching: 00:03:44 Overall time: 00:03:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3349648 / 15299262 = 0.2189 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:26:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:26:57: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:26:57: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:27:02: 1000000 INFO @ Sat, 11 Dec 2021 12:27:07: 2000000 INFO @ Sat, 11 Dec 2021 12:27:12: 3000000 INFO @ Sat, 11 Dec 2021 12:27:17: 4000000 INFO @ Sat, 11 Dec 2021 12:27:22: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:27:26: 6000000 INFO @ Sat, 11 Dec 2021 12:27:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:27:27: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:27:27: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:27:32: 7000000 INFO @ Sat, 11 Dec 2021 12:27:33: 1000000 INFO @ Sat, 11 Dec 2021 12:27:37: 8000000 INFO @ Sat, 11 Dec 2021 12:27:39: 2000000 INFO @ Sat, 11 Dec 2021 12:27:43: 9000000 INFO @ Sat, 11 Dec 2021 12:27:44: 3000000 INFO @ Sat, 11 Dec 2021 12:27:49: 10000000 INFO @ Sat, 11 Dec 2021 12:27:50: 4000000 INFO @ Sat, 11 Dec 2021 12:27:54: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:27:55: 5000000 INFO @ Sat, 11 Dec 2021 12:27:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:27:57: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:27:57: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:28:00: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:28:00: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:28:00: #1 total tags in treatment: 11949614 INFO @ Sat, 11 Dec 2021 12:28:00: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:28:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:28:00: #1 tags after filtering in treatment: 11949510 INFO @ Sat, 11 Dec 2021 12:28:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:28:00: #1 finished! INFO @ Sat, 11 Dec 2021 12:28:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:28:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:28:01: #2 number of paired peaks: 56 WARNING @ Sat, 11 Dec 2021 12:28:01: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:28:01: Process for pairing-model is terminated! INFO @ Sat, 11 Dec 2021 12:28:01: 6000000 cut: /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:28:03: 1000000 INFO @ Sat, 11 Dec 2021 12:28:07: 7000000 INFO @ Sat, 11 Dec 2021 12:28:10: 2000000 INFO @ Sat, 11 Dec 2021 12:28:12: 8000000 INFO @ Sat, 11 Dec 2021 12:28:16: 3000000 INFO @ Sat, 11 Dec 2021 12:28:18: 9000000 INFO @ Sat, 11 Dec 2021 12:28:22: 4000000 INFO @ Sat, 11 Dec 2021 12:28:24: 10000000 INFO @ Sat, 11 Dec 2021 12:28:28: 5000000 INFO @ Sat, 11 Dec 2021 12:28:29: 11000000 INFO @ Sat, 11 Dec 2021 12:28:34: 6000000 INFO @ Sat, 11 Dec 2021 12:28:35: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:28:35: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:28:35: #1 total tags in treatment: 11949614 INFO @ Sat, 11 Dec 2021 12:28:35: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:28:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:28:35: #1 tags after filtering in treatment: 11949510 INFO @ Sat, 11 Dec 2021 12:28:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:28:35: #1 finished! INFO @ Sat, 11 Dec 2021 12:28:35: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:28:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:28:36: #2 number of paired peaks: 56 WARNING @ Sat, 11 Dec 2021 12:28:36: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:28:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:28:40: 7000000 INFO @ Sat, 11 Dec 2021 12:28:46: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:28:52: 9000000 INFO @ Sat, 11 Dec 2021 12:28:58: 10000000 INFO @ Sat, 11 Dec 2021 12:29:04: 11000000 INFO @ Sat, 11 Dec 2021 12:29:10: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:29:10: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:29:10: #1 total tags in treatment: 11949614 INFO @ Sat, 11 Dec 2021 12:29:10: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:29:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:29:10: #1 tags after filtering in treatment: 11949510 INFO @ Sat, 11 Dec 2021 12:29:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:29:10: #1 finished! INFO @ Sat, 11 Dec 2021 12:29:10: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:29:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:29:11: #2 number of paired peaks: 56 WARNING @ Sat, 11 Dec 2021 12:29:11: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:29:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986113/SRX9986113.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。