Job ID = 14171661 SRX = SRX9986107 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:42 30099526 reads; of these: 30099526 (100.00%) were unpaired; of these: 2555635 (8.49%) aligned 0 times 18673721 (62.04%) aligned exactly 1 time 8870170 (29.47%) aligned >1 times 91.51% overall alignment rate Time searching: 00:17:42 Overall time: 00:17:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13273679 / 27543891 = 0.4819 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:44:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:44:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:44:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:44:25: 1000000 INFO @ Sat, 11 Dec 2021 12:44:31: 2000000 INFO @ Sat, 11 Dec 2021 12:44:37: 3000000 INFO @ Sat, 11 Dec 2021 12:44:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:44:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:44:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:44:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:44:51: 5000000 INFO @ Sat, 11 Dec 2021 12:44:55: 1000000 INFO @ Sat, 11 Dec 2021 12:44:57: 6000000 INFO @ Sat, 11 Dec 2021 12:45:02: 2000000 INFO @ Sat, 11 Dec 2021 12:45:04: 7000000 INFO @ Sat, 11 Dec 2021 12:45:09: 3000000 INFO @ Sat, 11 Dec 2021 12:45:11: 8000000 BedGraph に変換中... INFO @ Sat, 11 Dec 2021 12:45:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:45:17: 9000000 INFO @ Sat, 11 Dec 2021 12:45:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:45:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:45:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:45:23: 5000000 INFO @ Sat, 11 Dec 2021 12:45:25: 10000000 INFO @ Sat, 11 Dec 2021 12:45:26: 1000000 INFO @ Sat, 11 Dec 2021 12:45:30: 6000000 INFO @ Sat, 11 Dec 2021 12:45:31: 11000000 INFO @ Sat, 11 Dec 2021 12:45:34: 2000000 INFO @ Sat, 11 Dec 2021 12:45:38: 12000000 INFO @ Sat, 11 Dec 2021 12:45:38: 7000000 INFO @ Sat, 11 Dec 2021 12:45:41: 3000000 INFO @ Sat, 11 Dec 2021 12:45:46: 13000000 INFO @ Sat, 11 Dec 2021 12:45:46: 8000000 INFO @ Sat, 11 Dec 2021 12:45:49: 4000000 INFO @ Sat, 11 Dec 2021 12:45:52: 14000000 INFO @ Sat, 11 Dec 2021 12:45:54: 9000000 INFO @ Sat, 11 Dec 2021 12:45:55: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 12:45:55: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 12:45:55: #1 total tags in treatment: 14270212 INFO @ Sat, 11 Dec 2021 12:45:55: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:45:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:45:55: #1 tags after filtering in treatment: 14270113 INFO @ Sat, 11 Dec 2021 12:45:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:45:55: #1 finished! INFO @ Sat, 11 Dec 2021 12:45:55: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:45:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:45:56: #2 number of paired peaks: 19 WARNING @ Sat, 11 Dec 2021 12:45:56: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:45:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:45:57: 5000000 INFO @ Sat, 11 Dec 2021 12:46:01: 10000000 INFO @ Sat, 11 Dec 2021 12:46:04: 6000000 INFO @ Sat, 11 Dec 2021 12:46:09: 11000000 INFO @ Sat, 11 Dec 2021 12:46:12: 7000000 INFO @ Sat, 11 Dec 2021 12:46:16: 12000000 INFO @ Sat, 11 Dec 2021 12:46:19: 8000000 INFO @ Sat, 11 Dec 2021 12:46:25: 13000000 INFO @ Sat, 11 Dec 2021 12:46:26: 9000000 INFO @ Sat, 11 Dec 2021 12:46:32: 14000000 INFO @ Sat, 11 Dec 2021 12:46:33: 10000000 INFO @ Sat, 11 Dec 2021 12:46:34: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 12:46:34: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 12:46:34: #1 total tags in treatment: 14270212 INFO @ Sat, 11 Dec 2021 12:46:34: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:46:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:46:35: #1 tags after filtering in treatment: 14270113 INFO @ Sat, 11 Dec 2021 12:46:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:46:35: #1 finished! INFO @ Sat, 11 Dec 2021 12:46:35: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:46:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:46:36: #2 number of paired peaks: 19 WARNING @ Sat, 11 Dec 2021 12:46:36: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:46:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:46:40: 11000000 INFO @ Sat, 11 Dec 2021 12:46:47: 12000000 INFO @ Sat, 11 Dec 2021 12:46:54: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:47:01: 14000000 INFO @ Sat, 11 Dec 2021 12:47:03: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 12:47:03: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 12:47:03: #1 total tags in treatment: 14270212 INFO @ Sat, 11 Dec 2021 12:47:03: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:47:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:47:03: #1 tags after filtering in treatment: 14270113 INFO @ Sat, 11 Dec 2021 12:47:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:47:03: #1 finished! INFO @ Sat, 11 Dec 2021 12:47:03: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:47:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:47:04: #2 number of paired peaks: 19 WARNING @ Sat, 11 Dec 2021 12:47:04: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:47:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986107/SRX9986107.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。