Job ID = 14171685 SRX = SRX9986102 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:26 32797421 reads; of these: 32797421 (100.00%) were unpaired; of these: 1880845 (5.73%) aligned 0 times 22013230 (67.12%) aligned exactly 1 time 8903346 (27.15%) aligned >1 times 94.27% overall alignment rate Time searching: 00:14:26 Overall time: 00:14:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10703381 / 30916576 = 0.3462 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:48:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:48:25: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:48:25: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:48:31: 1000000 INFO @ Sat, 11 Dec 2021 12:48:37: 2000000 INFO @ Sat, 11 Dec 2021 12:48:43: 3000000 INFO @ Sat, 11 Dec 2021 12:48:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:48:55: 5000000 INFO @ Sat, 11 Dec 2021 12:48:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:48:55: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:48:55: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:49:02: 6000000 INFO @ Sat, 11 Dec 2021 12:49:02: 1000000 INFO @ Sat, 11 Dec 2021 12:49:09: 7000000 INFO @ Sat, 11 Dec 2021 12:49:09: 2000000 INFO @ Sat, 11 Dec 2021 12:49:16: 8000000 INFO @ Sat, 11 Dec 2021 12:49:16: 3000000 INFO @ Sat, 11 Dec 2021 12:49:22: 9000000 INFO @ Sat, 11 Dec 2021 12:49:23: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:49:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:49:25: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:49:25: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:49:30: 10000000 INFO @ Sat, 11 Dec 2021 12:49:30: 5000000 INFO @ Sat, 11 Dec 2021 12:49:33: 1000000 INFO @ Sat, 11 Dec 2021 12:49:37: 11000000 INFO @ Sat, 11 Dec 2021 12:49:38: 6000000 INFO @ Sat, 11 Dec 2021 12:49:42: 2000000 INFO @ Sat, 11 Dec 2021 12:49:45: 12000000 INFO @ Sat, 11 Dec 2021 12:49:45: 7000000 INFO @ Sat, 11 Dec 2021 12:49:50: 3000000 INFO @ Sat, 11 Dec 2021 12:49:52: 13000000 INFO @ Sat, 11 Dec 2021 12:49:53: 8000000 INFO @ Sat, 11 Dec 2021 12:49:58: 4000000 INFO @ Sat, 11 Dec 2021 12:50:00: 14000000 INFO @ Sat, 11 Dec 2021 12:50:00: 9000000 INFO @ Sat, 11 Dec 2021 12:50:06: 5000000 INFO @ Sat, 11 Dec 2021 12:50:07: 15000000 INFO @ Sat, 11 Dec 2021 12:50:08: 10000000 INFO @ Sat, 11 Dec 2021 12:50:14: 16000000 INFO @ Sat, 11 Dec 2021 12:50:15: 6000000 INFO @ Sat, 11 Dec 2021 12:50:15: 11000000 INFO @ Sat, 11 Dec 2021 12:50:22: 17000000 INFO @ Sat, 11 Dec 2021 12:50:23: 12000000 INFO @ Sat, 11 Dec 2021 12:50:23: 7000000 INFO @ Sat, 11 Dec 2021 12:50:30: 18000000 INFO @ Sat, 11 Dec 2021 12:50:30: 13000000 INFO @ Sat, 11 Dec 2021 12:50:31: 8000000 INFO @ Sat, 11 Dec 2021 12:50:37: 19000000 INFO @ Sat, 11 Dec 2021 12:50:37: 14000000 INFO @ Sat, 11 Dec 2021 12:50:39: 9000000 INFO @ Sat, 11 Dec 2021 12:50:45: 20000000 INFO @ Sat, 11 Dec 2021 12:50:45: 15000000 INFO @ Sat, 11 Dec 2021 12:50:46: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 12:50:46: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 12:50:46: #1 total tags in treatment: 20213195 INFO @ Sat, 11 Dec 2021 12:50:46: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:50:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:50:47: #1 tags after filtering in treatment: 20213125 INFO @ Sat, 11 Dec 2021 12:50:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:50:47: #1 finished! INFO @ Sat, 11 Dec 2021 12:50:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:50:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:50:47: 10000000 INFO @ Sat, 11 Dec 2021 12:50:48: #2 number of paired peaks: 4 WARNING @ Sat, 11 Dec 2021 12:50:48: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:50:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:50:52: 16000000 INFO @ Sat, 11 Dec 2021 12:50:55: 11000000 INFO @ Sat, 11 Dec 2021 12:50:59: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:51:04: 12000000 INFO @ Sat, 11 Dec 2021 12:51:07: 18000000 INFO @ Sat, 11 Dec 2021 12:51:12: 13000000 INFO @ Sat, 11 Dec 2021 12:51:14: 19000000 INFO @ Sat, 11 Dec 2021 12:51:20: 14000000 INFO @ Sat, 11 Dec 2021 12:51:21: 20000000 INFO @ Sat, 11 Dec 2021 12:51:23: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 12:51:23: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 12:51:23: #1 total tags in treatment: 20213195 INFO @ Sat, 11 Dec 2021 12:51:23: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:51:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:51:23: #1 tags after filtering in treatment: 20213125 INFO @ Sat, 11 Dec 2021 12:51:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:51:23: #1 finished! INFO @ Sat, 11 Dec 2021 12:51:23: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:51:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:51:24: #2 number of paired peaks: 4 WARNING @ Sat, 11 Dec 2021 12:51:24: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:51:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:51:28: 15000000 INFO @ Sat, 11 Dec 2021 12:51:35: 16000000 INFO @ Sat, 11 Dec 2021 12:51:43: 17000000 INFO @ Sat, 11 Dec 2021 12:51:51: 18000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 12:51:58: 19000000 INFO @ Sat, 11 Dec 2021 12:52:05: 20000000 INFO @ Sat, 11 Dec 2021 12:52:07: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 12:52:07: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 12:52:07: #1 total tags in treatment: 20213195 INFO @ Sat, 11 Dec 2021 12:52:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:52:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:52:08: #1 tags after filtering in treatment: 20213125 INFO @ Sat, 11 Dec 2021 12:52:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:52:08: #1 finished! INFO @ Sat, 11 Dec 2021 12:52:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:52:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:52:09: #2 number of paired peaks: 4 WARNING @ Sat, 11 Dec 2021 12:52:09: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:52:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986102/SRX9986102.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling