Job ID = 14171668 SRX = SRX9986101 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:05 41088924 reads; of these: 41088924 (100.00%) were unpaired; of these: 3938859 (9.59%) aligned 0 times 26463896 (64.41%) aligned exactly 1 time 10686169 (26.01%) aligned >1 times 90.41% overall alignment rate Time searching: 00:17:05 Overall time: 00:17:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 12801022 / 37150065 = 0.3446 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:48:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:48:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:48:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:48:10: 1000000 INFO @ Sat, 11 Dec 2021 12:48:15: 2000000 INFO @ Sat, 11 Dec 2021 12:48:21: 3000000 INFO @ Sat, 11 Dec 2021 12:48:27: 4000000 INFO @ Sat, 11 Dec 2021 12:48:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:48:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:48:34: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:48:34: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:48:38: 6000000 INFO @ Sat, 11 Dec 2021 12:48:40: 1000000 INFO @ Sat, 11 Dec 2021 12:48:44: 7000000 INFO @ Sat, 11 Dec 2021 12:48:46: 2000000 INFO @ Sat, 11 Dec 2021 12:48:50: 8000000 INFO @ Sat, 11 Dec 2021 12:48:53: 3000000 INFO @ Sat, 11 Dec 2021 12:48:56: 9000000 INFO @ Sat, 11 Dec 2021 12:48:59: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:49:02: 10000000 INFO @ Sat, 11 Dec 2021 12:49:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:49:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:49:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:49:05: 5000000 INFO @ Sat, 11 Dec 2021 12:49:09: 11000000 INFO @ Sat, 11 Dec 2021 12:49:11: 6000000 INFO @ Sat, 11 Dec 2021 12:49:12: 1000000 INFO @ Sat, 11 Dec 2021 12:49:15: 12000000 INFO @ Sat, 11 Dec 2021 12:49:18: 7000000 INFO @ Sat, 11 Dec 2021 12:49:19: 2000000 INFO @ Sat, 11 Dec 2021 12:49:22: 13000000 INFO @ Sat, 11 Dec 2021 12:49:25: 8000000 INFO @ Sat, 11 Dec 2021 12:49:27: 3000000 INFO @ Sat, 11 Dec 2021 12:49:29: 14000000 INFO @ Sat, 11 Dec 2021 12:49:31: 9000000 INFO @ Sat, 11 Dec 2021 12:49:34: 4000000 INFO @ Sat, 11 Dec 2021 12:49:35: 15000000 INFO @ Sat, 11 Dec 2021 12:49:38: 10000000 INFO @ Sat, 11 Dec 2021 12:49:42: 5000000 INFO @ Sat, 11 Dec 2021 12:49:42: 16000000 INFO @ Sat, 11 Dec 2021 12:49:44: 11000000 INFO @ Sat, 11 Dec 2021 12:49:49: 17000000 INFO @ Sat, 11 Dec 2021 12:49:49: 6000000 INFO @ Sat, 11 Dec 2021 12:49:51: 12000000 INFO @ Sat, 11 Dec 2021 12:49:55: 18000000 INFO @ Sat, 11 Dec 2021 12:49:57: 7000000 INFO @ Sat, 11 Dec 2021 12:49:58: 13000000 INFO @ Sat, 11 Dec 2021 12:50:02: 19000000 INFO @ Sat, 11 Dec 2021 12:50:04: 8000000 INFO @ Sat, 11 Dec 2021 12:50:05: 14000000 INFO @ Sat, 11 Dec 2021 12:50:09: 20000000 INFO @ Sat, 11 Dec 2021 12:50:11: 15000000 INFO @ Sat, 11 Dec 2021 12:50:12: 9000000 INFO @ Sat, 11 Dec 2021 12:50:16: 21000000 INFO @ Sat, 11 Dec 2021 12:50:18: 16000000 INFO @ Sat, 11 Dec 2021 12:50:19: 10000000 INFO @ Sat, 11 Dec 2021 12:50:22: 22000000 INFO @ Sat, 11 Dec 2021 12:50:25: 17000000 INFO @ Sat, 11 Dec 2021 12:50:27: 11000000 INFO @ Sat, 11 Dec 2021 12:50:29: 23000000 INFO @ Sat, 11 Dec 2021 12:50:31: 18000000 INFO @ Sat, 11 Dec 2021 12:50:34: 12000000 INFO @ Sat, 11 Dec 2021 12:50:36: 24000000 INFO @ Sat, 11 Dec 2021 12:50:38: 19000000 INFO @ Sat, 11 Dec 2021 12:50:38: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 12:50:38: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 12:50:38: #1 total tags in treatment: 24349043 INFO @ Sat, 11 Dec 2021 12:50:38: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:50:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:50:39: #1 tags after filtering in treatment: 24348987 INFO @ Sat, 11 Dec 2021 12:50:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:50:39: #1 finished! INFO @ Sat, 11 Dec 2021 12:50:39: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:50:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:50:40: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 12:50:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:50:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:50:42: 13000000 INFO @ Sat, 11 Dec 2021 12:50:45: 20000000 INFO @ Sat, 11 Dec 2021 12:50:49: 14000000 INFO @ Sat, 11 Dec 2021 12:50:51: 21000000 INFO @ Sat, 11 Dec 2021 12:50:57: 15000000 INFO @ Sat, 11 Dec 2021 12:50:58: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:51:04: 16000000 INFO @ Sat, 11 Dec 2021 12:51:05: 23000000 INFO @ Sat, 11 Dec 2021 12:51:11: 24000000 INFO @ Sat, 11 Dec 2021 12:51:12: 17000000 INFO @ Sat, 11 Dec 2021 12:51:14: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 12:51:14: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 12:51:14: #1 total tags in treatment: 24349043 INFO @ Sat, 11 Dec 2021 12:51:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:51:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:51:15: #1 tags after filtering in treatment: 24348987 INFO @ Sat, 11 Dec 2021 12:51:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:51:15: #1 finished! INFO @ Sat, 11 Dec 2021 12:51:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:51:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:51:16: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 12:51:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:51:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:51:19: 18000000 INFO @ Sat, 11 Dec 2021 12:51:26: 19000000 INFO @ Sat, 11 Dec 2021 12:51:33: 20000000 INFO @ Sat, 11 Dec 2021 12:51:40: 21000000 INFO @ Sat, 11 Dec 2021 12:51:47: 22000000 INFO @ Sat, 11 Dec 2021 12:51:54: 23000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 12:52:01: 24000000 INFO @ Sat, 11 Dec 2021 12:52:04: #1 tag size is determined as 58 bps INFO @ Sat, 11 Dec 2021 12:52:04: #1 tag size = 58 INFO @ Sat, 11 Dec 2021 12:52:04: #1 total tags in treatment: 24349043 INFO @ Sat, 11 Dec 2021 12:52:04: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:52:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:52:05: #1 tags after filtering in treatment: 24348987 INFO @ Sat, 11 Dec 2021 12:52:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:52:05: #1 finished! INFO @ Sat, 11 Dec 2021 12:52:05: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:52:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:52:06: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 12:52:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:52:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9986101/SRX9986101.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling