Job ID = 14169863 SRX = SRX9910773 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:27:03 61764665 reads; of these: 61764665 (100.00%) were unpaired; of these: 5042494 (8.16%) aligned 0 times 40412537 (65.43%) aligned exactly 1 time 16309634 (26.41%) aligned >1 times 91.84% overall alignment rate Time searching: 00:27:04 Overall time: 00:27:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 13184749 / 56722171 = 0.2324 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 04:34:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 04:34:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 04:34:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 04:34:19: 1000000 INFO @ Sat, 11 Dec 2021 04:34:26: 2000000 INFO @ Sat, 11 Dec 2021 04:34:33: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 04:34:41: 4000000 INFO @ Sat, 11 Dec 2021 04:34:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 04:34:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 04:34:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 04:34:49: 5000000 INFO @ Sat, 11 Dec 2021 04:34:50: 1000000 INFO @ Sat, 11 Dec 2021 04:34:57: 6000000 INFO @ Sat, 11 Dec 2021 04:34:58: 2000000 INFO @ Sat, 11 Dec 2021 04:35:06: 7000000 INFO @ Sat, 11 Dec 2021 04:35:06: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 04:35:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 04:35:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 04:35:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 04:35:14: 8000000 INFO @ Sat, 11 Dec 2021 04:35:14: 4000000 INFO @ Sat, 11 Dec 2021 04:35:20: 1000000 INFO @ Sat, 11 Dec 2021 04:35:23: 5000000 INFO @ Sat, 11 Dec 2021 04:35:23: 9000000 INFO @ Sat, 11 Dec 2021 04:35:29: 2000000 INFO @ Sat, 11 Dec 2021 04:35:31: 6000000 INFO @ Sat, 11 Dec 2021 04:35:31: 10000000 INFO @ Sat, 11 Dec 2021 04:35:38: 3000000 INFO @ Sat, 11 Dec 2021 04:35:39: 7000000 INFO @ Sat, 11 Dec 2021 04:35:40: 11000000 INFO @ Sat, 11 Dec 2021 04:35:46: 4000000 INFO @ Sat, 11 Dec 2021 04:35:48: 8000000 INFO @ Sat, 11 Dec 2021 04:35:48: 12000000 INFO @ Sat, 11 Dec 2021 04:35:55: 5000000 INFO @ Sat, 11 Dec 2021 04:35:56: 9000000 INFO @ Sat, 11 Dec 2021 04:35:56: 13000000 INFO @ Sat, 11 Dec 2021 04:36:04: 6000000 INFO @ Sat, 11 Dec 2021 04:36:05: 10000000 INFO @ Sat, 11 Dec 2021 04:36:05: 14000000 INFO @ Sat, 11 Dec 2021 04:36:12: 7000000 INFO @ Sat, 11 Dec 2021 04:36:13: 15000000 INFO @ Sat, 11 Dec 2021 04:36:14: 11000000 INFO @ Sat, 11 Dec 2021 04:36:21: 8000000 INFO @ Sat, 11 Dec 2021 04:36:21: 16000000 INFO @ Sat, 11 Dec 2021 04:36:22: 12000000 INFO @ Sat, 11 Dec 2021 04:36:29: 17000000 INFO @ Sat, 11 Dec 2021 04:36:30: 9000000 INFO @ Sat, 11 Dec 2021 04:36:30: 13000000 INFO @ Sat, 11 Dec 2021 04:36:38: 18000000 INFO @ Sat, 11 Dec 2021 04:36:38: 10000000 INFO @ Sat, 11 Dec 2021 04:36:39: 14000000 INFO @ Sat, 11 Dec 2021 04:36:46: 19000000 INFO @ Sat, 11 Dec 2021 04:36:47: 15000000 INFO @ Sat, 11 Dec 2021 04:36:47: 11000000 INFO @ Sat, 11 Dec 2021 04:36:54: 20000000 INFO @ Sat, 11 Dec 2021 04:36:56: 16000000 INFO @ Sat, 11 Dec 2021 04:36:56: 12000000 INFO @ Sat, 11 Dec 2021 04:37:03: 21000000 INFO @ Sat, 11 Dec 2021 04:37:04: 17000000 INFO @ Sat, 11 Dec 2021 04:37:04: 13000000 INFO @ Sat, 11 Dec 2021 04:37:11: 22000000 INFO @ Sat, 11 Dec 2021 04:37:13: 14000000 INFO @ Sat, 11 Dec 2021 04:37:14: 18000000 INFO @ Sat, 11 Dec 2021 04:37:19: 23000000 INFO @ Sat, 11 Dec 2021 04:37:21: 15000000 INFO @ Sat, 11 Dec 2021 04:37:22: 19000000 INFO @ Sat, 11 Dec 2021 04:37:28: 24000000 INFO @ Sat, 11 Dec 2021 04:37:30: 16000000 INFO @ Sat, 11 Dec 2021 04:37:31: 20000000 INFO @ Sat, 11 Dec 2021 04:37:36: 25000000 INFO @ Sat, 11 Dec 2021 04:37:39: 17000000 INFO @ Sat, 11 Dec 2021 04:37:39: 21000000 INFO @ Sat, 11 Dec 2021 04:37:44: 26000000 INFO @ Sat, 11 Dec 2021 04:37:47: 22000000 INFO @ Sat, 11 Dec 2021 04:37:47: 18000000 INFO @ Sat, 11 Dec 2021 04:37:53: 27000000 INFO @ Sat, 11 Dec 2021 04:37:56: 23000000 INFO @ Sat, 11 Dec 2021 04:37:56: 19000000 INFO @ Sat, 11 Dec 2021 04:38:01: 28000000 INFO @ Sat, 11 Dec 2021 04:38:04: 24000000 INFO @ Sat, 11 Dec 2021 04:38:04: 20000000 INFO @ Sat, 11 Dec 2021 04:38:09: 29000000 INFO @ Sat, 11 Dec 2021 04:38:12: 25000000 INFO @ Sat, 11 Dec 2021 04:38:12: 21000000 INFO @ Sat, 11 Dec 2021 04:38:17: 30000000 INFO @ Sat, 11 Dec 2021 04:38:20: 26000000 INFO @ Sat, 11 Dec 2021 04:38:21: 22000000 INFO @ Sat, 11 Dec 2021 04:38:26: 31000000 INFO @ Sat, 11 Dec 2021 04:38:28: 27000000 INFO @ Sat, 11 Dec 2021 04:38:29: 23000000 INFO @ Sat, 11 Dec 2021 04:38:34: 32000000 INFO @ Sat, 11 Dec 2021 04:38:37: 28000000 INFO @ Sat, 11 Dec 2021 04:38:38: 24000000 INFO @ Sat, 11 Dec 2021 04:38:42: 33000000 INFO @ Sat, 11 Dec 2021 04:38:45: 29000000 INFO @ Sat, 11 Dec 2021 04:38:47: 25000000 INFO @ Sat, 11 Dec 2021 04:38:50: 34000000 INFO @ Sat, 11 Dec 2021 04:38:53: 30000000 INFO @ Sat, 11 Dec 2021 04:38:55: 26000000 INFO @ Sat, 11 Dec 2021 04:38:59: 35000000 INFO @ Sat, 11 Dec 2021 04:39:01: 31000000 INFO @ Sat, 11 Dec 2021 04:39:04: 27000000 INFO @ Sat, 11 Dec 2021 04:39:09: 36000000 INFO @ Sat, 11 Dec 2021 04:39:09: 32000000 INFO @ Sat, 11 Dec 2021 04:39:12: 28000000 INFO @ Sat, 11 Dec 2021 04:39:17: 37000000 INFO @ Sat, 11 Dec 2021 04:39:18: 33000000 INFO @ Sat, 11 Dec 2021 04:39:21: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 04:39:25: 38000000 INFO @ Sat, 11 Dec 2021 04:39:26: 34000000 INFO @ Sat, 11 Dec 2021 04:39:29: 30000000 INFO @ Sat, 11 Dec 2021 04:39:33: 39000000 INFO @ Sat, 11 Dec 2021 04:39:34: 35000000 INFO @ Sat, 11 Dec 2021 04:39:37: 31000000 INFO @ Sat, 11 Dec 2021 04:39:42: 40000000 INFO @ Sat, 11 Dec 2021 04:39:44: 36000000 INFO @ Sat, 11 Dec 2021 04:39:46: 32000000 INFO @ Sat, 11 Dec 2021 04:39:50: 41000000 INFO @ Sat, 11 Dec 2021 04:39:52: 37000000 INFO @ Sat, 11 Dec 2021 04:39:54: 33000000 INFO @ Sat, 11 Dec 2021 04:39:58: 42000000 INFO @ Sat, 11 Dec 2021 04:40:00: 38000000 INFO @ Sat, 11 Dec 2021 04:40:02: 34000000 INFO @ Sat, 11 Dec 2021 04:40:07: 43000000 INFO @ Sat, 11 Dec 2021 04:40:09: 39000000 INFO @ Sat, 11 Dec 2021 04:40:11: 35000000 INFO @ Sat, 11 Dec 2021 04:40:12: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 04:40:12: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 04:40:12: #1 total tags in treatment: 43537422 INFO @ Sat, 11 Dec 2021 04:40:12: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 04:40:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 04:40:14: #1 tags after filtering in treatment: 43537422 INFO @ Sat, 11 Dec 2021 04:40:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 04:40:14: #1 finished! INFO @ Sat, 11 Dec 2021 04:40:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 04:40:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 04:40:16: #2 number of paired peaks: 56 WARNING @ Sat, 11 Dec 2021 04:40:16: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 04:40:16: Process for pairing-model is terminated! INFO @ Sat, 11 Dec 2021 04:40:17: 40000000 cut: /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 04:40:20: 36000000 INFO @ Sat, 11 Dec 2021 04:40:24: 41000000 INFO @ Sat, 11 Dec 2021 04:40:28: 37000000 INFO @ Sat, 11 Dec 2021 04:40:32: 42000000 INFO @ Sat, 11 Dec 2021 04:40:35: 38000000 INFO @ Sat, 11 Dec 2021 04:40:41: 43000000 INFO @ Sat, 11 Dec 2021 04:40:43: 39000000 INFO @ Sat, 11 Dec 2021 04:40:45: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 04:40:45: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 04:40:45: #1 total tags in treatment: 43537422 INFO @ Sat, 11 Dec 2021 04:40:45: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 04:40:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 04:40:47: #1 tags after filtering in treatment: 43537422 INFO @ Sat, 11 Dec 2021 04:40:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 04:40:47: #1 finished! INFO @ Sat, 11 Dec 2021 04:40:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 04:40:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 04:40:49: #2 number of paired peaks: 56 WARNING @ Sat, 11 Dec 2021 04:40:49: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 04:40:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 04:40:51: 40000000 INFO @ Sat, 11 Dec 2021 04:40:58: 41000000 INFO @ Sat, 11 Dec 2021 04:41:05: 42000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 04:41:13: 43000000 INFO @ Sat, 11 Dec 2021 04:41:17: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 04:41:17: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 04:41:17: #1 total tags in treatment: 43537422 INFO @ Sat, 11 Dec 2021 04:41:17: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 04:41:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 04:41:19: #1 tags after filtering in treatment: 43537422 INFO @ Sat, 11 Dec 2021 04:41:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 04:41:19: #1 finished! INFO @ Sat, 11 Dec 2021 04:41:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 04:41:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 04:41:21: #2 number of paired peaks: 56 WARNING @ Sat, 11 Dec 2021 04:41:21: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 04:41:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910773/SRX9910773.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling