Job ID = 14169860 SRX = SRX9910771 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:06 74805909 reads; of these: 74805909 (100.00%) were unpaired; of these: 6590349 (8.81%) aligned 0 times 48546753 (64.90%) aligned exactly 1 time 19668807 (26.29%) aligned >1 times 91.19% overall alignment rate Time searching: 00:32:06 Overall time: 00:32:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 17086126 / 68215560 = 0.2505 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 04:38:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 04:38:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 04:38:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 04:38:38: 1000000 INFO @ Sat, 11 Dec 2021 04:38:44: 2000000 INFO @ Sat, 11 Dec 2021 04:38:49: 3000000 INFO @ Sat, 11 Dec 2021 04:38:55: 4000000 INFO @ Sat, 11 Dec 2021 04:39:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 04:39:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 04:39:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 04:39:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 04:39:06: 6000000 INFO @ Sat, 11 Dec 2021 04:39:09: 1000000 INFO @ Sat, 11 Dec 2021 04:39:12: 7000000 INFO @ Sat, 11 Dec 2021 04:39:14: 2000000 INFO @ Sat, 11 Dec 2021 04:39:18: 8000000 INFO @ Sat, 11 Dec 2021 04:39:20: 3000000 INFO @ Sat, 11 Dec 2021 04:39:24: 9000000 INFO @ Sat, 11 Dec 2021 04:39:26: 4000000 INFO @ Sat, 11 Dec 2021 04:39:30: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 04:39:32: 5000000 INFO @ Sat, 11 Dec 2021 04:39:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 04:39:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 04:39:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 04:39:36: 11000000 INFO @ Sat, 11 Dec 2021 04:39:38: 6000000 INFO @ Sat, 11 Dec 2021 04:39:39: 1000000 INFO @ Sat, 11 Dec 2021 04:39:42: 12000000 INFO @ Sat, 11 Dec 2021 04:39:44: 7000000 INFO @ Sat, 11 Dec 2021 04:39:45: 2000000 INFO @ Sat, 11 Dec 2021 04:39:47: 13000000 INFO @ Sat, 11 Dec 2021 04:39:49: 8000000 INFO @ Sat, 11 Dec 2021 04:39:51: 3000000 INFO @ Sat, 11 Dec 2021 04:39:53: 14000000 INFO @ Sat, 11 Dec 2021 04:39:55: 9000000 INFO @ Sat, 11 Dec 2021 04:39:57: 4000000 INFO @ Sat, 11 Dec 2021 04:39:59: 15000000 INFO @ Sat, 11 Dec 2021 04:40:01: 10000000 INFO @ Sat, 11 Dec 2021 04:40:04: 5000000 INFO @ Sat, 11 Dec 2021 04:40:05: 16000000 INFO @ Sat, 11 Dec 2021 04:40:07: 11000000 INFO @ Sat, 11 Dec 2021 04:40:10: 6000000 INFO @ Sat, 11 Dec 2021 04:40:11: 17000000 INFO @ Sat, 11 Dec 2021 04:40:13: 12000000 INFO @ Sat, 11 Dec 2021 04:40:16: 7000000 INFO @ Sat, 11 Dec 2021 04:40:17: 18000000 INFO @ Sat, 11 Dec 2021 04:40:19: 13000000 INFO @ Sat, 11 Dec 2021 04:40:22: 8000000 INFO @ Sat, 11 Dec 2021 04:40:23: 19000000 INFO @ Sat, 11 Dec 2021 04:40:25: 14000000 INFO @ Sat, 11 Dec 2021 04:40:29: 9000000 INFO @ Sat, 11 Dec 2021 04:40:29: 20000000 INFO @ Sat, 11 Dec 2021 04:40:30: 15000000 INFO @ Sat, 11 Dec 2021 04:40:35: 10000000 INFO @ Sat, 11 Dec 2021 04:40:35: 21000000 INFO @ Sat, 11 Dec 2021 04:40:36: 16000000 INFO @ Sat, 11 Dec 2021 04:40:40: 11000000 INFO @ Sat, 11 Dec 2021 04:40:41: 22000000 INFO @ Sat, 11 Dec 2021 04:40:42: 17000000 INFO @ Sat, 11 Dec 2021 04:40:46: 12000000 INFO @ Sat, 11 Dec 2021 04:40:47: 23000000 INFO @ Sat, 11 Dec 2021 04:40:48: 18000000 INFO @ Sat, 11 Dec 2021 04:40:52: 13000000 INFO @ Sat, 11 Dec 2021 04:40:53: 24000000 INFO @ Sat, 11 Dec 2021 04:40:54: 19000000 INFO @ Sat, 11 Dec 2021 04:40:58: 14000000 INFO @ Sat, 11 Dec 2021 04:40:59: 25000000 INFO @ Sat, 11 Dec 2021 04:41:00: 20000000 INFO @ Sat, 11 Dec 2021 04:41:04: 15000000 INFO @ Sat, 11 Dec 2021 04:41:05: 26000000 INFO @ Sat, 11 Dec 2021 04:41:06: 21000000 INFO @ Sat, 11 Dec 2021 04:41:10: 16000000 INFO @ Sat, 11 Dec 2021 04:41:11: 27000000 INFO @ Sat, 11 Dec 2021 04:41:12: 22000000 INFO @ Sat, 11 Dec 2021 04:41:16: 17000000 INFO @ Sat, 11 Dec 2021 04:41:17: 28000000 INFO @ Sat, 11 Dec 2021 04:41:18: 23000000 INFO @ Sat, 11 Dec 2021 04:41:21: 18000000 INFO @ Sat, 11 Dec 2021 04:41:23: 29000000 INFO @ Sat, 11 Dec 2021 04:41:24: 24000000 INFO @ Sat, 11 Dec 2021 04:41:27: 19000000 INFO @ Sat, 11 Dec 2021 04:41:28: 30000000 INFO @ Sat, 11 Dec 2021 04:41:29: 25000000 INFO @ Sat, 11 Dec 2021 04:41:33: 20000000 INFO @ Sat, 11 Dec 2021 04:41:34: 31000000 INFO @ Sat, 11 Dec 2021 04:41:35: 26000000 INFO @ Sat, 11 Dec 2021 04:41:39: 21000000 INFO @ Sat, 11 Dec 2021 04:41:40: 32000000 INFO @ Sat, 11 Dec 2021 04:41:41: 27000000 INFO @ Sat, 11 Dec 2021 04:41:45: 22000000 INFO @ Sat, 11 Dec 2021 04:41:46: 33000000 INFO @ Sat, 11 Dec 2021 04:41:47: 28000000 INFO @ Sat, 11 Dec 2021 04:41:51: 23000000 INFO @ Sat, 11 Dec 2021 04:41:52: 34000000 INFO @ Sat, 11 Dec 2021 04:41:53: 29000000 INFO @ Sat, 11 Dec 2021 04:41:57: 24000000 INFO @ Sat, 11 Dec 2021 04:41:57: 35000000 INFO @ Sat, 11 Dec 2021 04:41:59: 30000000 INFO @ Sat, 11 Dec 2021 04:42:02: 25000000 INFO @ Sat, 11 Dec 2021 04:42:03: 36000000 INFO @ Sat, 11 Dec 2021 04:42:04: 31000000 INFO @ Sat, 11 Dec 2021 04:42:08: 26000000 INFO @ Sat, 11 Dec 2021 04:42:09: 37000000 INFO @ Sat, 11 Dec 2021 04:42:10: 32000000 INFO @ Sat, 11 Dec 2021 04:42:14: 27000000 INFO @ Sat, 11 Dec 2021 04:42:15: 38000000 INFO @ Sat, 11 Dec 2021 04:42:16: 33000000 INFO @ Sat, 11 Dec 2021 04:42:20: 28000000 INFO @ Sat, 11 Dec 2021 04:42:20: 39000000 INFO @ Sat, 11 Dec 2021 04:42:22: 34000000 INFO @ Sat, 11 Dec 2021 04:42:26: 29000000 INFO @ Sat, 11 Dec 2021 04:42:26: 40000000 INFO @ Sat, 11 Dec 2021 04:42:28: 35000000 INFO @ Sat, 11 Dec 2021 04:42:32: 41000000 INFO @ Sat, 11 Dec 2021 04:42:32: 30000000 INFO @ Sat, 11 Dec 2021 04:42:33: 36000000 INFO @ Sat, 11 Dec 2021 04:42:38: 31000000 INFO @ Sat, 11 Dec 2021 04:42:38: 42000000 INFO @ Sat, 11 Dec 2021 04:42:39: 37000000 INFO @ Sat, 11 Dec 2021 04:42:43: 32000000 INFO @ Sat, 11 Dec 2021 04:42:44: 43000000 INFO @ Sat, 11 Dec 2021 04:42:45: 38000000 INFO @ Sat, 11 Dec 2021 04:42:49: 33000000 INFO @ Sat, 11 Dec 2021 04:42:50: 44000000 INFO @ Sat, 11 Dec 2021 04:42:50: 39000000 INFO @ Sat, 11 Dec 2021 04:42:55: 34000000 INFO @ Sat, 11 Dec 2021 04:42:56: 45000000 INFO @ Sat, 11 Dec 2021 04:42:56: 40000000 INFO @ Sat, 11 Dec 2021 04:43:01: 35000000 INFO @ Sat, 11 Dec 2021 04:43:02: 46000000 INFO @ Sat, 11 Dec 2021 04:43:02: 41000000 INFO @ Sat, 11 Dec 2021 04:43:06: 36000000 INFO @ Sat, 11 Dec 2021 04:43:08: 47000000 INFO @ Sat, 11 Dec 2021 04:43:08: 42000000 INFO @ Sat, 11 Dec 2021 04:43:12: 37000000 INFO @ Sat, 11 Dec 2021 04:43:13: 48000000 INFO @ Sat, 11 Dec 2021 04:43:14: 43000000 INFO @ Sat, 11 Dec 2021 04:43:18: 38000000 INFO @ Sat, 11 Dec 2021 04:43:19: 49000000 INFO @ Sat, 11 Dec 2021 04:43:20: 44000000 INFO @ Sat, 11 Dec 2021 04:43:24: 39000000 INFO @ Sat, 11 Dec 2021 04:43:26: 50000000 INFO @ Sat, 11 Dec 2021 04:43:26: 45000000 INFO @ Sat, 11 Dec 2021 04:43:30: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 04:43:32: 51000000 INFO @ Sat, 11 Dec 2021 04:43:32: 46000000 INFO @ Sat, 11 Dec 2021 04:43:33: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 04:43:33: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 04:43:33: #1 total tags in treatment: 51129434 INFO @ Sat, 11 Dec 2021 04:43:33: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 04:43:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 04:43:34: #1 tags after filtering in treatment: 51129434 INFO @ Sat, 11 Dec 2021 04:43:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 04:43:34: #1 finished! INFO @ Sat, 11 Dec 2021 04:43:34: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 04:43:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 04:43:35: 41000000 INFO @ Sat, 11 Dec 2021 04:43:37: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 04:43:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 04:43:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 04:43:38: 47000000 INFO @ Sat, 11 Dec 2021 04:43:42: 42000000 INFO @ Sat, 11 Dec 2021 04:43:44: 48000000 INFO @ Sat, 11 Dec 2021 04:43:48: 43000000 INFO @ Sat, 11 Dec 2021 04:43:50: 49000000 INFO @ Sat, 11 Dec 2021 04:43:54: 44000000 INFO @ Sat, 11 Dec 2021 04:43:56: 50000000 INFO @ Sat, 11 Dec 2021 04:44:00: 45000000 INFO @ Sat, 11 Dec 2021 04:44:02: 51000000 INFO @ Sat, 11 Dec 2021 04:44:03: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 04:44:03: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 04:44:03: #1 total tags in treatment: 51129434 INFO @ Sat, 11 Dec 2021 04:44:03: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 04:44:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 04:44:04: #1 tags after filtering in treatment: 51129434 INFO @ Sat, 11 Dec 2021 04:44:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 04:44:04: #1 finished! INFO @ Sat, 11 Dec 2021 04:44:04: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 04:44:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 04:44:05: 46000000 INFO @ Sat, 11 Dec 2021 04:44:07: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 04:44:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 04:44:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 04:44:11: 47000000 INFO @ Sat, 11 Dec 2021 04:44:17: 48000000 INFO @ Sat, 11 Dec 2021 04:44:23: 49000000 INFO @ Sat, 11 Dec 2021 04:44:29: 50000000 INFO @ Sat, 11 Dec 2021 04:44:35: 51000000 INFO @ Sat, 11 Dec 2021 04:44:36: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 04:44:36: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 04:44:36: #1 total tags in treatment: 51129434 INFO @ Sat, 11 Dec 2021 04:44:36: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 04:44:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 04:44:37: #1 tags after filtering in treatment: 51129434 INFO @ Sat, 11 Dec 2021 04:44:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 04:44:37: #1 finished! INFO @ Sat, 11 Dec 2021 04:44:37: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 04:44:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 04:44:41: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 04:44:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 04:44:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9910771/SRX9910771.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。