Job ID = 14169856 SRX = SRX9910770 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:03 50137796 reads; of these: 50137796 (100.00%) were unpaired; of these: 10804597 (21.55%) aligned 0 times 28632852 (57.11%) aligned exactly 1 time 10700347 (21.34%) aligned >1 times 78.45% overall alignment rate Time searching: 00:27:03 Overall time: 00:27:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 22509095 / 39333199 = 0.5723 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 04:25:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 04:25:52: #1 read tag files... INFO @ Sat, 11 Dec 2021 04:25:52: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 04:26:02: 1000000 INFO @ Sat, 11 Dec 2021 04:26:12: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 04:26:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 04:26:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 04:26:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 04:26:22: 3000000 INFO @ Sat, 11 Dec 2021 04:26:33: 4000000 INFO @ Sat, 11 Dec 2021 04:26:37: 1000000 INFO @ Sat, 11 Dec 2021 04:26:43: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 04:26:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 04:26:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 04:26:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 04:26:53: 2000000 INFO @ Sat, 11 Dec 2021 04:26:54: 6000000 INFO @ Sat, 11 Dec 2021 04:27:05: 7000000 INFO @ Sat, 11 Dec 2021 04:27:09: 1000000 INFO @ Sat, 11 Dec 2021 04:27:11: 3000000 INFO @ Sat, 11 Dec 2021 04:27:16: 8000000 INFO @ Sat, 11 Dec 2021 04:27:26: 9000000 INFO @ Sat, 11 Dec 2021 04:27:27: 2000000 INFO @ Sat, 11 Dec 2021 04:27:29: 4000000 INFO @ Sat, 11 Dec 2021 04:27:37: 10000000 INFO @ Sat, 11 Dec 2021 04:27:45: 3000000 INFO @ Sat, 11 Dec 2021 04:27:47: 5000000 INFO @ Sat, 11 Dec 2021 04:27:48: 11000000 INFO @ Sat, 11 Dec 2021 04:27:58: 12000000 INFO @ Sat, 11 Dec 2021 04:28:03: 4000000 INFO @ Sat, 11 Dec 2021 04:28:05: 6000000 INFO @ Sat, 11 Dec 2021 04:28:10: 13000000 INFO @ Sat, 11 Dec 2021 04:28:21: 5000000 INFO @ Sat, 11 Dec 2021 04:28:21: 14000000 INFO @ Sat, 11 Dec 2021 04:28:23: 7000000 INFO @ Sat, 11 Dec 2021 04:28:32: 15000000 INFO @ Sat, 11 Dec 2021 04:28:39: 6000000 INFO @ Sat, 11 Dec 2021 04:28:41: 8000000 INFO @ Sat, 11 Dec 2021 04:28:43: 16000000 INFO @ Sat, 11 Dec 2021 04:28:52: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 04:28:52: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 04:28:52: #1 total tags in treatment: 16824104 INFO @ Sat, 11 Dec 2021 04:28:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 04:28:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 04:28:53: #1 tags after filtering in treatment: 16824103 INFO @ Sat, 11 Dec 2021 04:28:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 04:28:53: #1 finished! INFO @ Sat, 11 Dec 2021 04:28:53: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 04:28:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 04:28:54: #2 number of paired peaks: 2059 INFO @ Sat, 11 Dec 2021 04:28:54: start model_add_line... INFO @ Sat, 11 Dec 2021 04:28:55: start X-correlation... INFO @ Sat, 11 Dec 2021 04:28:55: end of X-cor INFO @ Sat, 11 Dec 2021 04:28:55: #2 finished! INFO @ Sat, 11 Dec 2021 04:28:55: #2 predicted fragment length is 234 bps INFO @ Sat, 11 Dec 2021 04:28:55: #2 alternative fragment length(s) may be 234 bps INFO @ Sat, 11 Dec 2021 04:28:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.05_model.r INFO @ Sat, 11 Dec 2021 04:28:55: #3 Call peaks... INFO @ Sat, 11 Dec 2021 04:28:55: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 04:28:57: 7000000 INFO @ Sat, 11 Dec 2021 04:28:59: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 04:29:15: 8000000 INFO @ Sat, 11 Dec 2021 04:29:17: 10000000 INFO @ Sat, 11 Dec 2021 04:29:33: 9000000 INFO @ Sat, 11 Dec 2021 04:29:35: 11000000 INFO @ Sat, 11 Dec 2021 04:29:51: 10000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 04:29:52: 12000000 INFO @ Sat, 11 Dec 2021 04:29:55: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 04:30:09: 11000000 INFO @ Sat, 11 Dec 2021 04:30:10: 13000000 INFO @ Sat, 11 Dec 2021 04:30:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.05_peaks.xls INFO @ Sat, 11 Dec 2021 04:30:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 04:30:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.05_summits.bed INFO @ Sat, 11 Dec 2021 04:30:20: Done! pass1 - making usageList (883 chroms): 5 millis pass2 - checking and writing primary data (8318 records, 4 fields): 75 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 04:30:27: 12000000 INFO @ Sat, 11 Dec 2021 04:30:29: 14000000 INFO @ Sat, 11 Dec 2021 04:30:44: 13000000 INFO @ Sat, 11 Dec 2021 04:30:46: 15000000 INFO @ Sat, 11 Dec 2021 04:31:03: 14000000 INFO @ Sat, 11 Dec 2021 04:31:04: 16000000 INFO @ Sat, 11 Dec 2021 04:31:19: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 04:31:19: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 04:31:19: #1 total tags in treatment: 16824104 INFO @ Sat, 11 Dec 2021 04:31:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 04:31:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 04:31:19: #1 tags after filtering in treatment: 16824103 INFO @ Sat, 11 Dec 2021 04:31:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 04:31:19: #1 finished! INFO @ Sat, 11 Dec 2021 04:31:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 04:31:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 04:31:20: 15000000 INFO @ Sat, 11 Dec 2021 04:31:21: #2 number of paired peaks: 2059 INFO @ Sat, 11 Dec 2021 04:31:21: start model_add_line... INFO @ Sat, 11 Dec 2021 04:31:21: start X-correlation... INFO @ Sat, 11 Dec 2021 04:31:21: end of X-cor INFO @ Sat, 11 Dec 2021 04:31:21: #2 finished! INFO @ Sat, 11 Dec 2021 04:31:21: #2 predicted fragment length is 234 bps INFO @ Sat, 11 Dec 2021 04:31:21: #2 alternative fragment length(s) may be 234 bps INFO @ Sat, 11 Dec 2021 04:31:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.10_model.r INFO @ Sat, 11 Dec 2021 04:31:21: #3 Call peaks... INFO @ Sat, 11 Dec 2021 04:31:21: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 04:31:36: 16000000 INFO @ Sat, 11 Dec 2021 04:31:49: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 04:31:49: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 04:31:49: #1 total tags in treatment: 16824104 INFO @ Sat, 11 Dec 2021 04:31:49: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 04:31:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 04:31:49: #1 tags after filtering in treatment: 16824103 INFO @ Sat, 11 Dec 2021 04:31:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 04:31:49: #1 finished! INFO @ Sat, 11 Dec 2021 04:31:49: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 04:31:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 04:31:51: #2 number of paired peaks: 2059 INFO @ Sat, 11 Dec 2021 04:31:51: start model_add_line... INFO @ Sat, 11 Dec 2021 04:31:51: start X-correlation... INFO @ Sat, 11 Dec 2021 04:31:51: end of X-cor INFO @ Sat, 11 Dec 2021 04:31:51: #2 finished! INFO @ Sat, 11 Dec 2021 04:31:51: #2 predicted fragment length is 234 bps INFO @ Sat, 11 Dec 2021 04:31:51: #2 alternative fragment length(s) may be 234 bps INFO @ Sat, 11 Dec 2021 04:31:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.20_model.r INFO @ Sat, 11 Dec 2021 04:31:51: #3 Call peaks... INFO @ Sat, 11 Dec 2021 04:31:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 04:32:19: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 04:32:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.10_peaks.xls INFO @ Sat, 11 Dec 2021 04:32:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 04:32:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.10_summits.bed INFO @ Sat, 11 Dec 2021 04:32:42: Done! pass1 - making usageList (736 chroms): 3 millis pass2 - checking and writing primary data (6136 records, 4 fields): 38 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 04:32:49: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 04:33:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.20_peaks.xls INFO @ Sat, 11 Dec 2021 04:33:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 04:33:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9910770/SRX9910770.20_summits.bed INFO @ Sat, 11 Dec 2021 04:33:12: Done! pass1 - making usageList (570 chroms): 3 millis pass2 - checking and writing primary data (4394 records, 4 fields): 32 millis CompletedMACS2peakCalling