Job ID = 14169776 SRX = SRX9910768 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:38 25659929 reads; of these: 25659929 (100.00%) were unpaired; of these: 6202429 (24.17%) aligned 0 times 13826569 (53.88%) aligned exactly 1 time 5630931 (21.94%) aligned >1 times 75.83% overall alignment rate Time searching: 00:09:38 Overall time: 00:09:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6904818 / 19457500 = 0.3549 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:30:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:30:14: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:30:14: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:30:20: 1000000 INFO @ Sat, 11 Dec 2021 03:30:26: 2000000 INFO @ Sat, 11 Dec 2021 03:30:32: 3000000 INFO @ Sat, 11 Dec 2021 03:30:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:30:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:30:44: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:30:44: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:30:45: 5000000 INFO @ Sat, 11 Dec 2021 03:30:51: 1000000 INFO @ Sat, 11 Dec 2021 03:30:52: 6000000 INFO @ Sat, 11 Dec 2021 03:30:58: 2000000 INFO @ Sat, 11 Dec 2021 03:30:59: 7000000 INFO @ Sat, 11 Dec 2021 03:31:05: 3000000 INFO @ Sat, 11 Dec 2021 03:31:06: 8000000 INFO @ Sat, 11 Dec 2021 03:31:12: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:31:13: 9000000 INFO @ Sat, 11 Dec 2021 03:31:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:31:14: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:31:14: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:31:19: 5000000 INFO @ Sat, 11 Dec 2021 03:31:20: 10000000 INFO @ Sat, 11 Dec 2021 03:31:21: 1000000 INFO @ Sat, 11 Dec 2021 03:31:26: 6000000 INFO @ Sat, 11 Dec 2021 03:31:27: 11000000 INFO @ Sat, 11 Dec 2021 03:31:28: 2000000 INFO @ Sat, 11 Dec 2021 03:31:33: 7000000 INFO @ Sat, 11 Dec 2021 03:31:34: 12000000 INFO @ Sat, 11 Dec 2021 03:31:36: 3000000 INFO @ Sat, 11 Dec 2021 03:31:38: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:31:38: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:31:38: #1 total tags in treatment: 12552682 INFO @ Sat, 11 Dec 2021 03:31:38: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:31:39: #1 tags after filtering in treatment: 12552682 INFO @ Sat, 11 Dec 2021 03:31:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:31:39: #1 finished! INFO @ Sat, 11 Dec 2021 03:31:39: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:31:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:31:40: #2 number of paired peaks: 2718 INFO @ Sat, 11 Dec 2021 03:31:40: start model_add_line... INFO @ Sat, 11 Dec 2021 03:31:40: start X-correlation... INFO @ Sat, 11 Dec 2021 03:31:40: end of X-cor INFO @ Sat, 11 Dec 2021 03:31:40: #2 finished! INFO @ Sat, 11 Dec 2021 03:31:40: #2 predicted fragment length is 210 bps INFO @ Sat, 11 Dec 2021 03:31:40: #2 alternative fragment length(s) may be 210 bps INFO @ Sat, 11 Dec 2021 03:31:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.05_model.r INFO @ Sat, 11 Dec 2021 03:31:40: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:31:40: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:31:40: 8000000 INFO @ Sat, 11 Dec 2021 03:31:43: 4000000 INFO @ Sat, 11 Dec 2021 03:31:47: 9000000 INFO @ Sat, 11 Dec 2021 03:31:50: 5000000 INFO @ Sat, 11 Dec 2021 03:31:54: 10000000 INFO @ Sat, 11 Dec 2021 03:31:57: 6000000 INFO @ Sat, 11 Dec 2021 03:32:01: 11000000 INFO @ Sat, 11 Dec 2021 03:32:04: 7000000 INFO @ Sat, 11 Dec 2021 03:32:09: 12000000 INFO @ Sat, 11 Dec 2021 03:32:09: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:32:11: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 03:32:13: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:32:13: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:32:13: #1 total tags in treatment: 12552682 INFO @ Sat, 11 Dec 2021 03:32:13: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:32:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:32:13: #1 tags after filtering in treatment: 12552682 INFO @ Sat, 11 Dec 2021 03:32:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:32:13: #1 finished! INFO @ Sat, 11 Dec 2021 03:32:13: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:32:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:32:14: #2 number of paired peaks: 2718 INFO @ Sat, 11 Dec 2021 03:32:14: start model_add_line... INFO @ Sat, 11 Dec 2021 03:32:14: start X-correlation... INFO @ Sat, 11 Dec 2021 03:32:14: end of X-cor INFO @ Sat, 11 Dec 2021 03:32:14: #2 finished! INFO @ Sat, 11 Dec 2021 03:32:14: #2 predicted fragment length is 210 bps INFO @ Sat, 11 Dec 2021 03:32:14: #2 alternative fragment length(s) may be 210 bps INFO @ Sat, 11 Dec 2021 03:32:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.10_model.r INFO @ Sat, 11 Dec 2021 03:32:14: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:32:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:32:18: 9000000 INFO @ Sat, 11 Dec 2021 03:32:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.05_peaks.xls INFO @ Sat, 11 Dec 2021 03:32:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:32:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.05_summits.bed INFO @ Sat, 11 Dec 2021 03:32:23: Done! pass1 - making usageList (790 chroms): 2 millis pass2 - checking and writing primary data (7307 records, 4 fields): 26 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:32:25: 10000000 INFO @ Sat, 11 Dec 2021 03:32:31: 11000000 INFO @ Sat, 11 Dec 2021 03:32:37: 12000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 03:32:41: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:32:41: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:32:41: #1 total tags in treatment: 12552682 INFO @ Sat, 11 Dec 2021 03:32:41: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:32:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:32:41: #1 tags after filtering in treatment: 12552682 INFO @ Sat, 11 Dec 2021 03:32:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:32:41: #1 finished! INFO @ Sat, 11 Dec 2021 03:32:41: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:32:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:32:42: #2 number of paired peaks: 2718 INFO @ Sat, 11 Dec 2021 03:32:42: start model_add_line... INFO @ Sat, 11 Dec 2021 03:32:42: start X-correlation... INFO @ Sat, 11 Dec 2021 03:32:43: end of X-cor INFO @ Sat, 11 Dec 2021 03:32:43: #2 finished! INFO @ Sat, 11 Dec 2021 03:32:43: #2 predicted fragment length is 210 bps INFO @ Sat, 11 Dec 2021 03:32:43: #2 alternative fragment length(s) may be 210 bps INFO @ Sat, 11 Dec 2021 03:32:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.20_model.r INFO @ Sat, 11 Dec 2021 03:32:43: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:32:43: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:32:44: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:32:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.10_peaks.xls INFO @ Sat, 11 Dec 2021 03:32:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:32:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.10_summits.bed INFO @ Sat, 11 Dec 2021 03:32:58: Done! pass1 - making usageList (677 chroms): 2 millis pass2 - checking and writing primary data (5530 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:33:12: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:33:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.20_peaks.xls INFO @ Sat, 11 Dec 2021 03:33:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:33:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9910768/SRX9910768.20_summits.bed INFO @ Sat, 11 Dec 2021 03:33:26: Done! pass1 - making usageList (540 chroms): 1 millis pass2 - checking and writing primary data (4030 records, 4 fields): 18 millis CompletedMACS2peakCalling