Job ID = 14172623 SRX = SRX9891433 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:03 33040703 reads; of these: 33040703 (100.00%) were unpaired; of these: 1892683 (5.73%) aligned 0 times 27375379 (82.85%) aligned exactly 1 time 3772641 (11.42%) aligned >1 times 94.27% overall alignment rate Time searching: 00:08:03 Overall time: 00:08:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4704813 / 31148020 = 0.1510 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:29:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:29:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:29:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:29:25: 1000000 INFO @ Sat, 11 Dec 2021 16:29:32: 2000000 INFO @ Sat, 11 Dec 2021 16:29:38: 3000000 INFO @ Sat, 11 Dec 2021 16:29:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:29:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:29:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:29:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:29:50: 5000000 INFO @ Sat, 11 Dec 2021 16:29:56: 1000000 INFO @ Sat, 11 Dec 2021 16:29:56: 6000000 INFO @ Sat, 11 Dec 2021 16:30:02: 7000000 INFO @ Sat, 11 Dec 2021 16:30:02: 2000000 INFO @ Sat, 11 Dec 2021 16:30:08: 8000000 INFO @ Sat, 11 Dec 2021 16:30:09: 3000000 INFO @ Sat, 11 Dec 2021 16:30:15: 9000000 INFO @ Sat, 11 Dec 2021 16:30:15: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:30:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:30:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:30:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:30:21: 10000000 INFO @ Sat, 11 Dec 2021 16:30:22: 5000000 INFO @ Sat, 11 Dec 2021 16:30:25: 1000000 INFO @ Sat, 11 Dec 2021 16:30:27: 11000000 INFO @ Sat, 11 Dec 2021 16:30:29: 6000000 INFO @ Sat, 11 Dec 2021 16:30:31: 2000000 INFO @ Sat, 11 Dec 2021 16:30:34: 12000000 INFO @ Sat, 11 Dec 2021 16:30:35: 7000000 INFO @ Sat, 11 Dec 2021 16:30:36: 3000000 INFO @ Sat, 11 Dec 2021 16:30:40: 13000000 INFO @ Sat, 11 Dec 2021 16:30:42: 8000000 INFO @ Sat, 11 Dec 2021 16:30:42: 4000000 INFO @ Sat, 11 Dec 2021 16:30:46: 14000000 INFO @ Sat, 11 Dec 2021 16:30:47: 5000000 INFO @ Sat, 11 Dec 2021 16:30:48: 9000000 INFO @ Sat, 11 Dec 2021 16:30:53: 15000000 INFO @ Sat, 11 Dec 2021 16:30:53: 6000000 INFO @ Sat, 11 Dec 2021 16:30:55: 10000000 INFO @ Sat, 11 Dec 2021 16:30:59: 7000000 INFO @ Sat, 11 Dec 2021 16:30:59: 16000000 INFO @ Sat, 11 Dec 2021 16:31:01: 11000000 INFO @ Sat, 11 Dec 2021 16:31:04: 8000000 INFO @ Sat, 11 Dec 2021 16:31:06: 17000000 INFO @ Sat, 11 Dec 2021 16:31:08: 12000000 INFO @ Sat, 11 Dec 2021 16:31:10: 9000000 INFO @ Sat, 11 Dec 2021 16:31:12: 18000000 INFO @ Sat, 11 Dec 2021 16:31:15: 13000000 INFO @ Sat, 11 Dec 2021 16:31:15: 10000000 INFO @ Sat, 11 Dec 2021 16:31:19: 19000000 INFO @ Sat, 11 Dec 2021 16:31:21: 11000000 INFO @ Sat, 11 Dec 2021 16:31:21: 14000000 INFO @ Sat, 11 Dec 2021 16:31:25: 20000000 INFO @ Sat, 11 Dec 2021 16:31:27: 12000000 INFO @ Sat, 11 Dec 2021 16:31:28: 15000000 INFO @ Sat, 11 Dec 2021 16:31:31: 21000000 INFO @ Sat, 11 Dec 2021 16:31:32: 13000000 INFO @ Sat, 11 Dec 2021 16:31:34: 16000000 INFO @ Sat, 11 Dec 2021 16:31:38: 14000000 INFO @ Sat, 11 Dec 2021 16:31:38: 22000000 INFO @ Sat, 11 Dec 2021 16:31:41: 17000000 INFO @ Sat, 11 Dec 2021 16:31:44: 15000000 INFO @ Sat, 11 Dec 2021 16:31:45: 23000000 INFO @ Sat, 11 Dec 2021 16:31:47: 18000000 INFO @ Sat, 11 Dec 2021 16:31:49: 16000000 INFO @ Sat, 11 Dec 2021 16:31:51: 24000000 INFO @ Sat, 11 Dec 2021 16:31:54: 19000000 INFO @ Sat, 11 Dec 2021 16:31:55: 17000000 INFO @ Sat, 11 Dec 2021 16:31:58: 25000000 INFO @ Sat, 11 Dec 2021 16:32:00: 18000000 INFO @ Sat, 11 Dec 2021 16:32:00: 20000000 INFO @ Sat, 11 Dec 2021 16:32:04: 26000000 INFO @ Sat, 11 Dec 2021 16:32:06: 19000000 INFO @ Sat, 11 Dec 2021 16:32:07: 21000000 INFO @ Sat, 11 Dec 2021 16:32:07: #1 tag size is determined as 60 bps INFO @ Sat, 11 Dec 2021 16:32:07: #1 tag size = 60 INFO @ Sat, 11 Dec 2021 16:32:07: #1 total tags in treatment: 26443207 INFO @ Sat, 11 Dec 2021 16:32:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:32:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:32:08: #1 tags after filtering in treatment: 26443179 INFO @ Sat, 11 Dec 2021 16:32:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 16:32:08: #1 finished! INFO @ Sat, 11 Dec 2021 16:32:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:32:08: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 16:32:10: #2 number of paired peaks: 32 WARNING @ Sat, 11 Dec 2021 16:32:10: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 16:32:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:32:12: 20000000 INFO @ Sat, 11 Dec 2021 16:32:14: 22000000 INFO @ Sat, 11 Dec 2021 16:32:17: 21000000 INFO @ Sat, 11 Dec 2021 16:32:21: 23000000 INFO @ Sat, 11 Dec 2021 16:32:23: 22000000 INFO @ Sat, 11 Dec 2021 16:32:27: 24000000 INFO @ Sat, 11 Dec 2021 16:32:29: 23000000 INFO @ Sat, 11 Dec 2021 16:32:34: 25000000 INFO @ Sat, 11 Dec 2021 16:32:34: 24000000 INFO @ Sat, 11 Dec 2021 16:32:40: 25000000 INFO @ Sat, 11 Dec 2021 16:32:40: 26000000 INFO @ Sat, 11 Dec 2021 16:32:43: #1 tag size is determined as 60 bps INFO @ Sat, 11 Dec 2021 16:32:43: #1 tag size = 60 INFO @ Sat, 11 Dec 2021 16:32:43: #1 total tags in treatment: 26443207 INFO @ Sat, 11 Dec 2021 16:32:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:32:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:32:44: #1 tags after filtering in treatment: 26443179 INFO @ Sat, 11 Dec 2021 16:32:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 16:32:44: #1 finished! INFO @ Sat, 11 Dec 2021 16:32:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:32:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:32:45: 26000000 INFO @ Sat, 11 Dec 2021 16:32:46: #2 number of paired peaks: 32 WARNING @ Sat, 11 Dec 2021 16:32:46: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 16:32:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:32:48: #1 tag size is determined as 60 bps INFO @ Sat, 11 Dec 2021 16:32:48: #1 tag size = 60 INFO @ Sat, 11 Dec 2021 16:32:48: #1 total tags in treatment: 26443207 INFO @ Sat, 11 Dec 2021 16:32:48: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:32:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:32:49: #1 tags after filtering in treatment: 26443179 INFO @ Sat, 11 Dec 2021 16:32:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 16:32:49: #1 finished! INFO @ Sat, 11 Dec 2021 16:32:49: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:32:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:32:51: #2 number of paired peaks: 32 WARNING @ Sat, 11 Dec 2021 16:32:51: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 16:32:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9891433/SRX9891433.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。