Job ID = 14169636 SRX = SRX9730756 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:21 30360190 reads; of these: 30360190 (100.00%) were unpaired; of these: 826830 (2.72%) aligned 0 times 24212641 (79.75%) aligned exactly 1 time 5320719 (17.53%) aligned >1 times 97.28% overall alignment rate Time searching: 00:10:21 Overall time: 00:10:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15683445 / 29533360 = 0.5310 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:19:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:19:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:19:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:19:11: 1000000 INFO @ Sat, 11 Dec 2021 03:19:17: 2000000 INFO @ Sat, 11 Dec 2021 03:19:24: 3000000 INFO @ Sat, 11 Dec 2021 03:19:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:19:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:19:34: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:19:34: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:19:37: 5000000 INFO @ Sat, 11 Dec 2021 03:19:40: 1000000 INFO @ Sat, 11 Dec 2021 03:19:44: 6000000 INFO @ Sat, 11 Dec 2021 03:19:46: 2000000 INFO @ Sat, 11 Dec 2021 03:19:50: 7000000 INFO @ Sat, 11 Dec 2021 03:19:51: 3000000 INFO @ Sat, 11 Dec 2021 03:19:57: 4000000 INFO @ Sat, 11 Dec 2021 03:19:57: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:20:03: 5000000 INFO @ Sat, 11 Dec 2021 03:20:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:20:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:20:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:20:04: 9000000 INFO @ Sat, 11 Dec 2021 03:20:09: 6000000 INFO @ Sat, 11 Dec 2021 03:20:11: 1000000 INFO @ Sat, 11 Dec 2021 03:20:11: 10000000 INFO @ Sat, 11 Dec 2021 03:20:15: 7000000 INFO @ Sat, 11 Dec 2021 03:20:18: 2000000 INFO @ Sat, 11 Dec 2021 03:20:18: 11000000 INFO @ Sat, 11 Dec 2021 03:20:21: 8000000 INFO @ Sat, 11 Dec 2021 03:20:25: 3000000 INFO @ Sat, 11 Dec 2021 03:20:26: 12000000 INFO @ Sat, 11 Dec 2021 03:20:27: 9000000 INFO @ Sat, 11 Dec 2021 03:20:32: 4000000 INFO @ Sat, 11 Dec 2021 03:20:32: 10000000 INFO @ Sat, 11 Dec 2021 03:20:33: 13000000 INFO @ Sat, 11 Dec 2021 03:20:38: 11000000 INFO @ Sat, 11 Dec 2021 03:20:39: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:20:39: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:20:39: #1 total tags in treatment: 13849915 INFO @ Sat, 11 Dec 2021 03:20:39: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:20:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:20:39: 5000000 INFO @ Sat, 11 Dec 2021 03:20:39: #1 tags after filtering in treatment: 13849503 INFO @ Sat, 11 Dec 2021 03:20:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:20:39: #1 finished! INFO @ Sat, 11 Dec 2021 03:20:39: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:20:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:20:41: #2 number of paired peaks: 13246 INFO @ Sat, 11 Dec 2021 03:20:41: start model_add_line... INFO @ Sat, 11 Dec 2021 03:20:42: start X-correlation... INFO @ Sat, 11 Dec 2021 03:20:42: end of X-cor INFO @ Sat, 11 Dec 2021 03:20:42: #2 finished! INFO @ Sat, 11 Dec 2021 03:20:42: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 03:20:42: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 03:20:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.05_model.r INFO @ Sat, 11 Dec 2021 03:20:42: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:20:42: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:20:45: 12000000 INFO @ Sat, 11 Dec 2021 03:20:46: 6000000 INFO @ Sat, 11 Dec 2021 03:20:50: 13000000 INFO @ Sat, 11 Dec 2021 03:20:53: 7000000 INFO @ Sat, 11 Dec 2021 03:20:56: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:20:56: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:20:56: #1 total tags in treatment: 13849915 INFO @ Sat, 11 Dec 2021 03:20:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:20:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:20:56: #1 tags after filtering in treatment: 13849503 INFO @ Sat, 11 Dec 2021 03:20:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:20:56: #1 finished! INFO @ Sat, 11 Dec 2021 03:20:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:20:56: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 03:20:58: #2 number of paired peaks: 13246 INFO @ Sat, 11 Dec 2021 03:20:58: start model_add_line... INFO @ Sat, 11 Dec 2021 03:20:58: start X-correlation... INFO @ Sat, 11 Dec 2021 03:20:58: end of X-cor INFO @ Sat, 11 Dec 2021 03:20:58: #2 finished! INFO @ Sat, 11 Dec 2021 03:20:58: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 03:20:58: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 03:20:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.10_model.r INFO @ Sat, 11 Dec 2021 03:20:58: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:20:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:21:00: 8000000 INFO @ Sat, 11 Dec 2021 03:21:07: 9000000 INFO @ Sat, 11 Dec 2021 03:21:13: 10000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 03:21:20: 11000000 INFO @ Sat, 11 Dec 2021 03:21:27: 12000000 INFO @ Sat, 11 Dec 2021 03:21:33: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:21:33: 13000000 INFO @ Sat, 11 Dec 2021 03:21:39: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:21:39: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:21:39: #1 total tags in treatment: 13849915 INFO @ Sat, 11 Dec 2021 03:21:39: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:21:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:21:40: #1 tags after filtering in treatment: 13849503 INFO @ Sat, 11 Dec 2021 03:21:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:21:40: #1 finished! INFO @ Sat, 11 Dec 2021 03:21:40: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:21:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:21:41: #2 number of paired peaks: 13246 INFO @ Sat, 11 Dec 2021 03:21:41: start model_add_line... INFO @ Sat, 11 Dec 2021 03:21:42: start X-correlation... INFO @ Sat, 11 Dec 2021 03:21:42: end of X-cor INFO @ Sat, 11 Dec 2021 03:21:42: #2 finished! INFO @ Sat, 11 Dec 2021 03:21:42: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 03:21:42: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 03:21:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.20_model.r INFO @ Sat, 11 Dec 2021 03:21:42: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:21:42: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:21:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.05_peaks.xls INFO @ Sat, 11 Dec 2021 03:21:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:21:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.05_summits.bed INFO @ Sat, 11 Dec 2021 03:21:49: Done! INFO @ Sat, 11 Dec 2021 03:21:49: #3 Call peaks for each chromosome... pass1 - making usageList (163 chroms): 2 millis pass2 - checking and writing primary data (7908 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:22:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.10_peaks.xls INFO @ Sat, 11 Dec 2021 03:22:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:22:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.10_summits.bed INFO @ Sat, 11 Dec 2021 03:22:05: Done! pass1 - making usageList (150 chroms): 2 millis pass2 - checking and writing primary data (7291 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:22:33: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:22:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.20_peaks.xls INFO @ Sat, 11 Dec 2021 03:22:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:22:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730756/SRX9730756.20_summits.bed INFO @ Sat, 11 Dec 2021 03:22:49: Done! pass1 - making usageList (125 chroms): 2 millis pass2 - checking and writing primary data (6461 records, 4 fields): 11 millis CompletedMACS2peakCalling