Job ID = 14169631 SRX = SRX9730754 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:19 28025445 reads; of these: 28025445 (100.00%) were unpaired; of these: 771361 (2.75%) aligned 0 times 22239982 (79.36%) aligned exactly 1 time 5014102 (17.89%) aligned >1 times 97.25% overall alignment rate Time searching: 00:11:19 Overall time: 00:11:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14540643 / 27254084 = 0.5335 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:18:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:18:25: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:18:25: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:18:30: 1000000 INFO @ Sat, 11 Dec 2021 03:18:36: 2000000 INFO @ Sat, 11 Dec 2021 03:18:41: 3000000 INFO @ Sat, 11 Dec 2021 03:18:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:18:54: 5000000 INFO @ Sat, 11 Dec 2021 03:18:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:18:54: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:18:54: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:19:00: 6000000 INFO @ Sat, 11 Dec 2021 03:19:01: 1000000 INFO @ Sat, 11 Dec 2021 03:19:07: 7000000 INFO @ Sat, 11 Dec 2021 03:19:08: 2000000 INFO @ Sat, 11 Dec 2021 03:19:14: 8000000 INFO @ Sat, 11 Dec 2021 03:19:15: 3000000 INFO @ Sat, 11 Dec 2021 03:19:21: 9000000 INFO @ Sat, 11 Dec 2021 03:19:21: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:19:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:19:25: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:19:25: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:19:28: 10000000 INFO @ Sat, 11 Dec 2021 03:19:28: 5000000 INFO @ Sat, 11 Dec 2021 03:19:31: 1000000 INFO @ Sat, 11 Dec 2021 03:19:35: 6000000 INFO @ Sat, 11 Dec 2021 03:19:36: 11000000 INFO @ Sat, 11 Dec 2021 03:19:38: 2000000 INFO @ Sat, 11 Dec 2021 03:19:41: 7000000 INFO @ Sat, 11 Dec 2021 03:19:42: 12000000 INFO @ Sat, 11 Dec 2021 03:19:44: 3000000 INFO @ Sat, 11 Dec 2021 03:19:47: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:19:47: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:19:47: #1 total tags in treatment: 12713441 INFO @ Sat, 11 Dec 2021 03:19:47: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:19:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:19:48: 8000000 INFO @ Sat, 11 Dec 2021 03:19:48: #1 tags after filtering in treatment: 12713047 INFO @ Sat, 11 Dec 2021 03:19:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:19:48: #1 finished! INFO @ Sat, 11 Dec 2021 03:19:48: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:19:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:19:50: #2 number of paired peaks: 12854 INFO @ Sat, 11 Dec 2021 03:19:50: start model_add_line... INFO @ Sat, 11 Dec 2021 03:19:50: start X-correlation... INFO @ Sat, 11 Dec 2021 03:19:50: end of X-cor INFO @ Sat, 11 Dec 2021 03:19:50: #2 finished! INFO @ Sat, 11 Dec 2021 03:19:50: #2 predicted fragment length is 196 bps INFO @ Sat, 11 Dec 2021 03:19:50: #2 alternative fragment length(s) may be 196 bps INFO @ Sat, 11 Dec 2021 03:19:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.05_model.r INFO @ Sat, 11 Dec 2021 03:19:50: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:19:50: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:19:51: 4000000 INFO @ Sat, 11 Dec 2021 03:19:54: 9000000 INFO @ Sat, 11 Dec 2021 03:19:58: 5000000 INFO @ Sat, 11 Dec 2021 03:20:00: 10000000 INFO @ Sat, 11 Dec 2021 03:20:04: 6000000 INFO @ Sat, 11 Dec 2021 03:20:07: 11000000 INFO @ Sat, 11 Dec 2021 03:20:11: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 03:20:14: 12000000 INFO @ Sat, 11 Dec 2021 03:20:18: 8000000 INFO @ Sat, 11 Dec 2021 03:20:19: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:20:19: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:20:19: #1 total tags in treatment: 12713441 INFO @ Sat, 11 Dec 2021 03:20:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:20:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:20:19: #1 tags after filtering in treatment: 12713047 INFO @ Sat, 11 Dec 2021 03:20:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:20:19: #1 finished! INFO @ Sat, 11 Dec 2021 03:20:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:20:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:20:21: #2 number of paired peaks: 12854 INFO @ Sat, 11 Dec 2021 03:20:21: start model_add_line... INFO @ Sat, 11 Dec 2021 03:20:21: start X-correlation... INFO @ Sat, 11 Dec 2021 03:20:22: end of X-cor INFO @ Sat, 11 Dec 2021 03:20:22: #2 finished! INFO @ Sat, 11 Dec 2021 03:20:22: #2 predicted fragment length is 196 bps INFO @ Sat, 11 Dec 2021 03:20:22: #2 alternative fragment length(s) may be 196 bps INFO @ Sat, 11 Dec 2021 03:20:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.10_model.r INFO @ Sat, 11 Dec 2021 03:20:22: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:20:22: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:20:24: 9000000 INFO @ Sat, 11 Dec 2021 03:20:31: 10000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 03:20:35: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:20:38: 11000000 INFO @ Sat, 11 Dec 2021 03:20:45: 12000000 INFO @ Sat, 11 Dec 2021 03:20:50: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:20:50: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:20:50: #1 total tags in treatment: 12713441 INFO @ Sat, 11 Dec 2021 03:20:50: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:20:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:20:50: #1 tags after filtering in treatment: 12713047 INFO @ Sat, 11 Dec 2021 03:20:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:20:50: #1 finished! INFO @ Sat, 11 Dec 2021 03:20:50: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:20:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:20:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.05_peaks.xls INFO @ Sat, 11 Dec 2021 03:20:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:20:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.05_summits.bed INFO @ Sat, 11 Dec 2021 03:20:51: Done! pass1 - making usageList (169 chroms): 1 millis pass2 - checking and writing primary data (8117 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:20:52: #2 number of paired peaks: 12854 INFO @ Sat, 11 Dec 2021 03:20:52: start model_add_line... INFO @ Sat, 11 Dec 2021 03:20:52: start X-correlation... INFO @ Sat, 11 Dec 2021 03:20:53: end of X-cor INFO @ Sat, 11 Dec 2021 03:20:53: #2 finished! INFO @ Sat, 11 Dec 2021 03:20:53: #2 predicted fragment length is 196 bps INFO @ Sat, 11 Dec 2021 03:20:53: #2 alternative fragment length(s) may be 196 bps INFO @ Sat, 11 Dec 2021 03:20:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.20_model.r INFO @ Sat, 11 Dec 2021 03:20:53: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:20:53: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:21:07: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:21:22: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.10_peaks.xls INFO @ Sat, 11 Dec 2021 03:21:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:21:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.10_summits.bed INFO @ Sat, 11 Dec 2021 03:21:22: Done! pass1 - making usageList (150 chroms): 2 millis pass2 - checking and writing primary data (7398 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:21:39: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:21:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.20_peaks.xls INFO @ Sat, 11 Dec 2021 03:21:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:21:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730754/SRX9730754.20_summits.bed INFO @ Sat, 11 Dec 2021 03:21:57: Done! pass1 - making usageList (125 chroms): 2 millis pass2 - checking and writing primary data (6564 records, 4 fields): 12 millis CompletedMACS2peakCalling