Job ID = 14169634 SRX = SRX9730753 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:14:11 38738351 reads; of these: 38738351 (100.00%) were unpaired; of these: 1115313 (2.88%) aligned 0 times 30531003 (78.81%) aligned exactly 1 time 7092035 (18.31%) aligned >1 times 97.12% overall alignment rate Time searching: 00:14:12 Overall time: 00:14:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 16494644 / 37623038 = 0.4384 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:24:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:24:06: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:24:06: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:24:11: 1000000 INFO @ Sat, 11 Dec 2021 03:24:17: 2000000 INFO @ Sat, 11 Dec 2021 03:24:22: 3000000 INFO @ Sat, 11 Dec 2021 03:24:27: 4000000 INFO @ Sat, 11 Dec 2021 03:24:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:24:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:24:36: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:24:36: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:24:37: 6000000 INFO @ Sat, 11 Dec 2021 03:24:41: 1000000 INFO @ Sat, 11 Dec 2021 03:24:42: 7000000 INFO @ Sat, 11 Dec 2021 03:24:46: 2000000 INFO @ Sat, 11 Dec 2021 03:24:48: 8000000 INFO @ Sat, 11 Dec 2021 03:24:52: 3000000 INFO @ Sat, 11 Dec 2021 03:24:53: 9000000 INFO @ Sat, 11 Dec 2021 03:24:57: 4000000 INFO @ Sat, 11 Dec 2021 03:24:59: 10000000 INFO @ Sat, 11 Dec 2021 03:25:03: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:25:04: 11000000 INFO @ Sat, 11 Dec 2021 03:25:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:25:06: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:25:06: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:25:08: 6000000 INFO @ Sat, 11 Dec 2021 03:25:09: 12000000 INFO @ Sat, 11 Dec 2021 03:25:12: 1000000 INFO @ Sat, 11 Dec 2021 03:25:14: 7000000 INFO @ Sat, 11 Dec 2021 03:25:15: 13000000 INFO @ Sat, 11 Dec 2021 03:25:18: 2000000 INFO @ Sat, 11 Dec 2021 03:25:19: 8000000 INFO @ Sat, 11 Dec 2021 03:25:21: 14000000 INFO @ Sat, 11 Dec 2021 03:25:24: 3000000 INFO @ Sat, 11 Dec 2021 03:25:25: 9000000 INFO @ Sat, 11 Dec 2021 03:25:26: 15000000 INFO @ Sat, 11 Dec 2021 03:25:30: 10000000 INFO @ Sat, 11 Dec 2021 03:25:31: 4000000 INFO @ Sat, 11 Dec 2021 03:25:32: 16000000 INFO @ Sat, 11 Dec 2021 03:25:36: 11000000 INFO @ Sat, 11 Dec 2021 03:25:37: 5000000 INFO @ Sat, 11 Dec 2021 03:25:37: 17000000 INFO @ Sat, 11 Dec 2021 03:25:41: 12000000 INFO @ Sat, 11 Dec 2021 03:25:43: 6000000 INFO @ Sat, 11 Dec 2021 03:25:43: 18000000 INFO @ Sat, 11 Dec 2021 03:25:47: 13000000 INFO @ Sat, 11 Dec 2021 03:25:49: 19000000 INFO @ Sat, 11 Dec 2021 03:25:49: 7000000 INFO @ Sat, 11 Dec 2021 03:25:52: 14000000 INFO @ Sat, 11 Dec 2021 03:25:54: 20000000 INFO @ Sat, 11 Dec 2021 03:25:55: 8000000 INFO @ Sat, 11 Dec 2021 03:25:58: 15000000 INFO @ Sat, 11 Dec 2021 03:26:00: 21000000 INFO @ Sat, 11 Dec 2021 03:26:01: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:26:01: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:26:01: #1 total tags in treatment: 21128394 INFO @ Sat, 11 Dec 2021 03:26:01: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:26:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:26:01: #1 tags after filtering in treatment: 21128008 INFO @ Sat, 11 Dec 2021 03:26:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:26:01: #1 finished! INFO @ Sat, 11 Dec 2021 03:26:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:26:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:26:02: 9000000 INFO @ Sat, 11 Dec 2021 03:26:04: 16000000 INFO @ Sat, 11 Dec 2021 03:26:04: #2 number of paired peaks: 10384 INFO @ Sat, 11 Dec 2021 03:26:04: start model_add_line... INFO @ Sat, 11 Dec 2021 03:26:04: start X-correlation... INFO @ Sat, 11 Dec 2021 03:26:04: end of X-cor INFO @ Sat, 11 Dec 2021 03:26:04: #2 finished! INFO @ Sat, 11 Dec 2021 03:26:04: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 03:26:04: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 03:26:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.05_model.r INFO @ Sat, 11 Dec 2021 03:26:04: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:26:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:26:08: 10000000 INFO @ Sat, 11 Dec 2021 03:26:09: 17000000 INFO @ Sat, 11 Dec 2021 03:26:14: 11000000 INFO @ Sat, 11 Dec 2021 03:26:15: 18000000 INFO @ Sat, 11 Dec 2021 03:26:20: 12000000 INFO @ Sat, 11 Dec 2021 03:26:20: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 03:26:26: 20000000 INFO @ Sat, 11 Dec 2021 03:26:27: 13000000 INFO @ Sat, 11 Dec 2021 03:26:32: 21000000 INFO @ Sat, 11 Dec 2021 03:26:32: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:26:32: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:26:32: #1 total tags in treatment: 21128394 INFO @ Sat, 11 Dec 2021 03:26:32: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:26:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:26:33: 14000000 INFO @ Sat, 11 Dec 2021 03:26:33: #1 tags after filtering in treatment: 21128008 INFO @ Sat, 11 Dec 2021 03:26:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:26:33: #1 finished! INFO @ Sat, 11 Dec 2021 03:26:33: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:26:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:26:35: #2 number of paired peaks: 10384 INFO @ Sat, 11 Dec 2021 03:26:35: start model_add_line... INFO @ Sat, 11 Dec 2021 03:26:36: start X-correlation... INFO @ Sat, 11 Dec 2021 03:26:36: end of X-cor INFO @ Sat, 11 Dec 2021 03:26:36: #2 finished! INFO @ Sat, 11 Dec 2021 03:26:36: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 03:26:36: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 03:26:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.10_model.r INFO @ Sat, 11 Dec 2021 03:26:36: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:26:36: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:26:39: 15000000 INFO @ Sat, 11 Dec 2021 03:26:45: 16000000 INFO @ Sat, 11 Dec 2021 03:26:51: 17000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 03:26:57: 18000000 INFO @ Sat, 11 Dec 2021 03:27:03: 19000000 INFO @ Sat, 11 Dec 2021 03:27:08: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:27:09: 20000000 INFO @ Sat, 11 Dec 2021 03:27:15: 21000000 INFO @ Sat, 11 Dec 2021 03:27:16: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:27:16: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:27:16: #1 total tags in treatment: 21128394 INFO @ Sat, 11 Dec 2021 03:27:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:27:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:27:17: #1 tags after filtering in treatment: 21128008 INFO @ Sat, 11 Dec 2021 03:27:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:27:17: #1 finished! INFO @ Sat, 11 Dec 2021 03:27:17: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:27:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:27:19: #2 number of paired peaks: 10384 INFO @ Sat, 11 Dec 2021 03:27:19: start model_add_line... INFO @ Sat, 11 Dec 2021 03:27:19: start X-correlation... INFO @ Sat, 11 Dec 2021 03:27:19: end of X-cor INFO @ Sat, 11 Dec 2021 03:27:19: #2 finished! INFO @ Sat, 11 Dec 2021 03:27:19: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 03:27:19: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 03:27:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.20_model.r INFO @ Sat, 11 Dec 2021 03:27:19: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:27:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:27:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.05_peaks.xls INFO @ Sat, 11 Dec 2021 03:27:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:27:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.05_summits.bed INFO @ Sat, 11 Dec 2021 03:27:29: Done! pass1 - making usageList (159 chroms): 2 millis pass2 - checking and writing primary data (9335 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:27:39: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:28:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.10_peaks.xls INFO @ Sat, 11 Dec 2021 03:28:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:28:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.10_summits.bed INFO @ Sat, 11 Dec 2021 03:28:00: Done! pass1 - making usageList (146 chroms): 1 millis pass2 - checking and writing primary data (7939 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:28:25: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:28:45: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.20_peaks.xls INFO @ Sat, 11 Dec 2021 03:28:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:28:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730753/SRX9730753.20_summits.bed INFO @ Sat, 11 Dec 2021 03:28:45: Done! pass1 - making usageList (132 chroms): 2 millis pass2 - checking and writing primary data (5990 records, 4 fields): 11 millis CompletedMACS2peakCalling