Job ID = 14169621 SRX = SRX9730751 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:45 23339864 reads; of these: 23339864 (100.00%) were unpaired; of these: 607342 (2.60%) aligned 0 times 18215267 (78.04%) aligned exactly 1 time 4517255 (19.35%) aligned >1 times 97.40% overall alignment rate Time searching: 00:07:45 Overall time: 00:07:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8534477 / 22732522 = 0.3754 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:08:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:08:44: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:08:44: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:08:50: 1000000 INFO @ Sat, 11 Dec 2021 03:08:56: 2000000 INFO @ Sat, 11 Dec 2021 03:09:02: 3000000 INFO @ Sat, 11 Dec 2021 03:09:07: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:09:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:09:13: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:09:13: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:09:13: 5000000 INFO @ Sat, 11 Dec 2021 03:09:21: 6000000 INFO @ Sat, 11 Dec 2021 03:09:21: 1000000 INFO @ Sat, 11 Dec 2021 03:09:28: 7000000 INFO @ Sat, 11 Dec 2021 03:09:28: 2000000 INFO @ Sat, 11 Dec 2021 03:09:35: 8000000 INFO @ Sat, 11 Dec 2021 03:09:35: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 03:09:42: 9000000 INFO @ Sat, 11 Dec 2021 03:09:42: 4000000 INFO @ Sat, 11 Dec 2021 03:09:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 03:09:43: #1 read tag files... INFO @ Sat, 11 Dec 2021 03:09:43: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 03:09:50: 10000000 INFO @ Sat, 11 Dec 2021 03:09:50: 5000000 INFO @ Sat, 11 Dec 2021 03:09:52: 1000000 INFO @ Sat, 11 Dec 2021 03:09:57: 11000000 INFO @ Sat, 11 Dec 2021 03:09:57: 6000000 INFO @ Sat, 11 Dec 2021 03:10:00: 2000000 INFO @ Sat, 11 Dec 2021 03:10:05: 7000000 INFO @ Sat, 11 Dec 2021 03:10:05: 12000000 INFO @ Sat, 11 Dec 2021 03:10:09: 3000000 INFO @ Sat, 11 Dec 2021 03:10:12: 8000000 INFO @ Sat, 11 Dec 2021 03:10:12: 13000000 INFO @ Sat, 11 Dec 2021 03:10:18: 4000000 INFO @ Sat, 11 Dec 2021 03:10:20: 14000000 INFO @ Sat, 11 Dec 2021 03:10:20: 9000000 INFO @ Sat, 11 Dec 2021 03:10:21: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:10:21: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:10:21: #1 total tags in treatment: 14198045 INFO @ Sat, 11 Dec 2021 03:10:21: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:10:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:10:22: #1 tags after filtering in treatment: 14197562 INFO @ Sat, 11 Dec 2021 03:10:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:10:22: #1 finished! INFO @ Sat, 11 Dec 2021 03:10:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:10:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:10:24: #2 number of paired peaks: 10793 INFO @ Sat, 11 Dec 2021 03:10:24: start model_add_line... INFO @ Sat, 11 Dec 2021 03:10:24: start X-correlation... INFO @ Sat, 11 Dec 2021 03:10:24: end of X-cor INFO @ Sat, 11 Dec 2021 03:10:24: #2 finished! INFO @ Sat, 11 Dec 2021 03:10:24: #2 predicted fragment length is 194 bps INFO @ Sat, 11 Dec 2021 03:10:24: #2 alternative fragment length(s) may be 194 bps INFO @ Sat, 11 Dec 2021 03:10:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.05_model.r INFO @ Sat, 11 Dec 2021 03:10:24: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:10:24: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:10:26: 5000000 INFO @ Sat, 11 Dec 2021 03:10:27: 10000000 INFO @ Sat, 11 Dec 2021 03:10:35: 11000000 INFO @ Sat, 11 Dec 2021 03:10:35: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 03:10:42: 12000000 INFO @ Sat, 11 Dec 2021 03:10:43: 7000000 INFO @ Sat, 11 Dec 2021 03:10:50: 13000000 INFO @ Sat, 11 Dec 2021 03:10:52: 8000000 INFO @ Sat, 11 Dec 2021 03:10:57: 14000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 03:10:59: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:10:59: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:10:59: #1 total tags in treatment: 14198045 INFO @ Sat, 11 Dec 2021 03:10:59: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:10:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:10:59: #1 tags after filtering in treatment: 14197562 INFO @ Sat, 11 Dec 2021 03:10:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:10:59: #1 finished! INFO @ Sat, 11 Dec 2021 03:10:59: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:10:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:11:00: 9000000 INFO @ Sat, 11 Dec 2021 03:11:01: #2 number of paired peaks: 10793 INFO @ Sat, 11 Dec 2021 03:11:01: start model_add_line... INFO @ Sat, 11 Dec 2021 03:11:01: start X-correlation... INFO @ Sat, 11 Dec 2021 03:11:01: end of X-cor INFO @ Sat, 11 Dec 2021 03:11:01: #2 finished! INFO @ Sat, 11 Dec 2021 03:11:01: #2 predicted fragment length is 194 bps INFO @ Sat, 11 Dec 2021 03:11:01: #2 alternative fragment length(s) may be 194 bps INFO @ Sat, 11 Dec 2021 03:11:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.10_model.r INFO @ Sat, 11 Dec 2021 03:11:01: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:11:01: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:11:07: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:11:08: 10000000 INFO @ Sat, 11 Dec 2021 03:11:16: 11000000 INFO @ Sat, 11 Dec 2021 03:11:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.05_peaks.xls INFO @ Sat, 11 Dec 2021 03:11:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:11:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.05_summits.bed INFO @ Sat, 11 Dec 2021 03:11:22: Done! pass1 - making usageList (160 chroms): 2 millis pass2 - checking and writing primary data (8718 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:11:24: 12000000 INFO @ Sat, 11 Dec 2021 03:11:31: 13000000 INFO @ Sat, 11 Dec 2021 03:11:39: 14000000 INFO @ Sat, 11 Dec 2021 03:11:41: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 03:11:41: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 03:11:41: #1 total tags in treatment: 14198045 INFO @ Sat, 11 Dec 2021 03:11:41: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 03:11:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 03:11:41: #1 tags after filtering in treatment: 14197562 INFO @ Sat, 11 Dec 2021 03:11:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 03:11:41: #1 finished! INFO @ Sat, 11 Dec 2021 03:11:41: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 03:11:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 03:11:43: #2 number of paired peaks: 10793 INFO @ Sat, 11 Dec 2021 03:11:43: start model_add_line... INFO @ Sat, 11 Dec 2021 03:11:43: start X-correlation... INFO @ Sat, 11 Dec 2021 03:11:43: end of X-cor INFO @ Sat, 11 Dec 2021 03:11:43: #2 finished! INFO @ Sat, 11 Dec 2021 03:11:43: #2 predicted fragment length is 194 bps INFO @ Sat, 11 Dec 2021 03:11:43: #2 alternative fragment length(s) may be 194 bps INFO @ Sat, 11 Dec 2021 03:11:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.20_model.r INFO @ Sat, 11 Dec 2021 03:11:43: #3 Call peaks... INFO @ Sat, 11 Dec 2021 03:11:43: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 03:11:45: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:12:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.10_peaks.xls INFO @ Sat, 11 Dec 2021 03:12:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:12:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.10_summits.bed INFO @ Sat, 11 Dec 2021 03:12:00: Done! pass1 - making usageList (149 chroms): 2 millis pass2 - checking and writing primary data (7435 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 03:12:27: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 03:12:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.20_peaks.xls INFO @ Sat, 11 Dec 2021 03:12:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 03:12:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730751/SRX9730751.20_summits.bed INFO @ Sat, 11 Dec 2021 03:12:42: Done! pass1 - making usageList (128 chroms): 2 millis pass2 - checking and writing primary data (5920 records, 4 fields): 10 millis CompletedMACS2peakCalling