Job ID = 14169616 SRX = SRX9730750 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:05 27590513 reads; of these: 27590513 (100.00%) were unpaired; of these: 1142294 (4.14%) aligned 0 times 21066347 (76.35%) aligned exactly 1 time 5381872 (19.51%) aligned >1 times 95.86% overall alignment rate Time searching: 00:10:05 Overall time: 00:10:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4792866 / 26448219 = 0.1812 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 02:36:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 02:36:10: #1 read tag files... INFO @ Sat, 11 Dec 2021 02:36:10: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 02:36:16: 1000000 INFO @ Sat, 11 Dec 2021 02:36:21: 2000000 INFO @ Sat, 11 Dec 2021 02:36:27: 3000000 INFO @ Sat, 11 Dec 2021 02:36:32: 4000000 INFO @ Sat, 11 Dec 2021 02:36:38: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 02:36:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 02:36:40: #1 read tag files... INFO @ Sat, 11 Dec 2021 02:36:40: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 02:36:43: 6000000 INFO @ Sat, 11 Dec 2021 02:36:46: 1000000 INFO @ Sat, 11 Dec 2021 02:36:49: 7000000 INFO @ Sat, 11 Dec 2021 02:36:52: 2000000 INFO @ Sat, 11 Dec 2021 02:36:55: 8000000 INFO @ Sat, 11 Dec 2021 02:36:57: 3000000 INFO @ Sat, 11 Dec 2021 02:37:00: 9000000 INFO @ Sat, 11 Dec 2021 02:37:04: 4000000 INFO @ Sat, 11 Dec 2021 02:37:06: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 02:37:09: 5000000 INFO @ Sat, 11 Dec 2021 02:37:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 02:37:10: #1 read tag files... INFO @ Sat, 11 Dec 2021 02:37:10: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 02:37:11: 11000000 INFO @ Sat, 11 Dec 2021 02:37:15: 6000000 INFO @ Sat, 11 Dec 2021 02:37:16: 1000000 INFO @ Sat, 11 Dec 2021 02:37:17: 12000000 INFO @ Sat, 11 Dec 2021 02:37:21: 7000000 INFO @ Sat, 11 Dec 2021 02:37:22: 2000000 INFO @ Sat, 11 Dec 2021 02:37:23: 13000000 INFO @ Sat, 11 Dec 2021 02:37:27: 8000000 INFO @ Sat, 11 Dec 2021 02:37:28: 3000000 INFO @ Sat, 11 Dec 2021 02:37:28: 14000000 INFO @ Sat, 11 Dec 2021 02:37:33: 9000000 INFO @ Sat, 11 Dec 2021 02:37:34: 15000000 INFO @ Sat, 11 Dec 2021 02:37:34: 4000000 INFO @ Sat, 11 Dec 2021 02:37:38: 10000000 INFO @ Sat, 11 Dec 2021 02:37:39: 16000000 INFO @ Sat, 11 Dec 2021 02:37:40: 5000000 INFO @ Sat, 11 Dec 2021 02:37:44: 11000000 INFO @ Sat, 11 Dec 2021 02:37:45: 17000000 INFO @ Sat, 11 Dec 2021 02:37:46: 6000000 INFO @ Sat, 11 Dec 2021 02:37:49: 12000000 INFO @ Sat, 11 Dec 2021 02:37:51: 18000000 INFO @ Sat, 11 Dec 2021 02:37:51: 7000000 INFO @ Sat, 11 Dec 2021 02:37:55: 13000000 INFO @ Sat, 11 Dec 2021 02:37:56: 19000000 INFO @ Sat, 11 Dec 2021 02:37:57: 8000000 INFO @ Sat, 11 Dec 2021 02:38:01: 14000000 INFO @ Sat, 11 Dec 2021 02:38:02: 20000000 INFO @ Sat, 11 Dec 2021 02:38:02: 9000000 INFO @ Sat, 11 Dec 2021 02:38:06: 15000000 INFO @ Sat, 11 Dec 2021 02:38:07: 21000000 INFO @ Sat, 11 Dec 2021 02:38:08: 10000000 INFO @ Sat, 11 Dec 2021 02:38:11: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 02:38:11: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 02:38:11: #1 total tags in treatment: 21655353 INFO @ Sat, 11 Dec 2021 02:38:11: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 02:38:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 02:38:12: 16000000 INFO @ Sat, 11 Dec 2021 02:38:12: #1 tags after filtering in treatment: 21655194 INFO @ Sat, 11 Dec 2021 02:38:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 02:38:12: #1 finished! INFO @ Sat, 11 Dec 2021 02:38:12: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 02:38:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 02:38:13: 11000000 INFO @ Sat, 11 Dec 2021 02:38:13: #2 number of paired peaks: 1808 INFO @ Sat, 11 Dec 2021 02:38:13: start model_add_line... INFO @ Sat, 11 Dec 2021 02:38:14: start X-correlation... INFO @ Sat, 11 Dec 2021 02:38:14: end of X-cor INFO @ Sat, 11 Dec 2021 02:38:14: #2 finished! INFO @ Sat, 11 Dec 2021 02:38:14: #2 predicted fragment length is 71 bps INFO @ Sat, 11 Dec 2021 02:38:14: #2 alternative fragment length(s) may be 1,71 bps INFO @ Sat, 11 Dec 2021 02:38:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.05_model.r WARNING @ Sat, 11 Dec 2021 02:38:14: #2 Since the d (71) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 02:38:14: #2 You may need to consider one of the other alternative d(s): 1,71 WARNING @ Sat, 11 Dec 2021 02:38:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 02:38:14: #3 Call peaks... INFO @ Sat, 11 Dec 2021 02:38:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 02:38:17: 17000000 INFO @ Sat, 11 Dec 2021 02:38:19: 12000000 INFO @ Sat, 11 Dec 2021 02:38:23: 18000000 INFO @ Sat, 11 Dec 2021 02:38:24: 13000000 INFO @ Sat, 11 Dec 2021 02:38:28: 19000000 INFO @ Sat, 11 Dec 2021 02:38:30: 14000000 INFO @ Sat, 11 Dec 2021 02:38:34: 20000000 INFO @ Sat, 11 Dec 2021 02:38:35: 15000000 INFO @ Sat, 11 Dec 2021 02:38:39: 21000000 INFO @ Sat, 11 Dec 2021 02:38:41: 16000000 INFO @ Sat, 11 Dec 2021 02:38:43: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 02:38:43: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 02:38:43: #1 total tags in treatment: 21655353 INFO @ Sat, 11 Dec 2021 02:38:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 02:38:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 02:38:44: #1 tags after filtering in treatment: 21655194 INFO @ Sat, 11 Dec 2021 02:38:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 02:38:44: #1 finished! INFO @ Sat, 11 Dec 2021 02:38:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 02:38:44: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 02:38:45: #2 number of paired peaks: 1808 INFO @ Sat, 11 Dec 2021 02:38:45: start model_add_line... INFO @ Sat, 11 Dec 2021 02:38:46: start X-correlation... INFO @ Sat, 11 Dec 2021 02:38:46: end of X-cor INFO @ Sat, 11 Dec 2021 02:38:46: #2 finished! INFO @ Sat, 11 Dec 2021 02:38:46: #2 predicted fragment length is 71 bps INFO @ Sat, 11 Dec 2021 02:38:46: #2 alternative fragment length(s) may be 1,71 bps INFO @ Sat, 11 Dec 2021 02:38:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.10_model.r WARNING @ Sat, 11 Dec 2021 02:38:46: #2 Since the d (71) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 02:38:46: #2 You may need to consider one of the other alternative d(s): 1,71 WARNING @ Sat, 11 Dec 2021 02:38:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 02:38:46: #3 Call peaks... INFO @ Sat, 11 Dec 2021 02:38:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 02:38:46: 17000000 INFO @ Sat, 11 Dec 2021 02:38:52: 18000000 INFO @ Sat, 11 Dec 2021 02:38:53: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 02:38:57: 19000000 INFO @ Sat, 11 Dec 2021 02:39:03: 20000000 INFO @ Sat, 11 Dec 2021 02:39:08: 21000000 INFO @ Sat, 11 Dec 2021 02:39:12: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 02:39:12: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 02:39:12: #1 total tags in treatment: 21655353 INFO @ Sat, 11 Dec 2021 02:39:12: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 02:39:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 02:39:12: #1 tags after filtering in treatment: 21655194 INFO @ Sat, 11 Dec 2021 02:39:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 02:39:12: #1 finished! INFO @ Sat, 11 Dec 2021 02:39:12: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 02:39:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 02:39:14: #2 number of paired peaks: 1808 INFO @ Sat, 11 Dec 2021 02:39:14: start model_add_line... INFO @ Sat, 11 Dec 2021 02:39:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.05_peaks.xls INFO @ Sat, 11 Dec 2021 02:39:14: start X-correlation... INFO @ Sat, 11 Dec 2021 02:39:14: end of X-cor INFO @ Sat, 11 Dec 2021 02:39:14: #2 finished! INFO @ Sat, 11 Dec 2021 02:39:14: #2 predicted fragment length is 71 bps INFO @ Sat, 11 Dec 2021 02:39:14: #2 alternative fragment length(s) may be 1,71 bps INFO @ Sat, 11 Dec 2021 02:39:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.20_model.r INFO @ Sat, 11 Dec 2021 02:39:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.05_peaks.narrowPeak WARNING @ Sat, 11 Dec 2021 02:39:14: #2 Since the d (71) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 02:39:14: #2 You may need to consider one of the other alternative d(s): 1,71 WARNING @ Sat, 11 Dec 2021 02:39:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 02:39:14: #3 Call peaks... INFO @ Sat, 11 Dec 2021 02:39:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 02:39:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.05_summits.bed INFO @ Sat, 11 Dec 2021 02:39:14: Done! pass1 - making usageList (355 chroms): 1 millis pass2 - checking and writing primary data (3262 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 02:39:25: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 02:39:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.10_peaks.xls INFO @ Sat, 11 Dec 2021 02:39:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 02:39:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.10_summits.bed INFO @ Sat, 11 Dec 2021 02:39:46: Done! pass1 - making usageList (236 chroms): 1 millis pass2 - checking and writing primary data (1369 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 02:39:54: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 02:40:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.20_peaks.xls INFO @ Sat, 11 Dec 2021 02:40:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 02:40:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9730750/SRX9730750.20_summits.bed INFO @ Sat, 11 Dec 2021 02:40:14: Done! pass1 - making usageList (146 chroms): 1 millis pass2 - checking and writing primary data (472 records, 4 fields): 6 millis CompletedMACS2peakCalling