Job ID = 14170773 SRX = SRX9720909 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:12 22329782 reads; of these: 22329782 (100.00%) were unpaired; of these: 1023798 (4.58%) aligned 0 times 14846687 (66.49%) aligned exactly 1 time 6459297 (28.93%) aligned >1 times 95.42% overall alignment rate Time searching: 00:06:12 Overall time: 00:06:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3632691 / 21305984 = 0.1705 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:42:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:42:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:42:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:42:54: 1000000 INFO @ Sat, 11 Dec 2021 08:42:59: 2000000 INFO @ Sat, 11 Dec 2021 08:43:04: 3000000 INFO @ Sat, 11 Dec 2021 08:43:09: 4000000 INFO @ Sat, 11 Dec 2021 08:43:14: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:43:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:43:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:43:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:43:19: 6000000 INFO @ Sat, 11 Dec 2021 08:43:25: 7000000 INFO @ Sat, 11 Dec 2021 08:43:25: 1000000 INFO @ Sat, 11 Dec 2021 08:43:30: 8000000 INFO @ Sat, 11 Dec 2021 08:43:30: 2000000 INFO @ Sat, 11 Dec 2021 08:43:35: 9000000 INFO @ Sat, 11 Dec 2021 08:43:35: 3000000 INFO @ Sat, 11 Dec 2021 08:43:41: 10000000 INFO @ Sat, 11 Dec 2021 08:43:41: 4000000 INFO @ Sat, 11 Dec 2021 08:43:46: 11000000 INFO @ Sat, 11 Dec 2021 08:43:46: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:43:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:43:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:43:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:43:51: 12000000 INFO @ Sat, 11 Dec 2021 08:43:52: 6000000 INFO @ Sat, 11 Dec 2021 08:43:55: 1000000 INFO @ Sat, 11 Dec 2021 08:43:57: 13000000 INFO @ Sat, 11 Dec 2021 08:43:57: 7000000 INFO @ Sat, 11 Dec 2021 08:44:00: 2000000 INFO @ Sat, 11 Dec 2021 08:44:02: 14000000 INFO @ Sat, 11 Dec 2021 08:44:03: 8000000 INFO @ Sat, 11 Dec 2021 08:44:06: 3000000 INFO @ Sat, 11 Dec 2021 08:44:08: 15000000 INFO @ Sat, 11 Dec 2021 08:44:08: 9000000 INFO @ Sat, 11 Dec 2021 08:44:11: 4000000 INFO @ Sat, 11 Dec 2021 08:44:14: 16000000 INFO @ Sat, 11 Dec 2021 08:44:14: 10000000 INFO @ Sat, 11 Dec 2021 08:44:17: 5000000 INFO @ Sat, 11 Dec 2021 08:44:19: 17000000 INFO @ Sat, 11 Dec 2021 08:44:20: 11000000 INFO @ Sat, 11 Dec 2021 08:44:23: 6000000 INFO @ Sat, 11 Dec 2021 08:44:23: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:44:23: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:44:23: #1 total tags in treatment: 17673293 INFO @ Sat, 11 Dec 2021 08:44:23: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:44:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:44:24: #1 tags after filtering in treatment: 17673227 INFO @ Sat, 11 Dec 2021 08:44:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:44:24: #1 finished! INFO @ Sat, 11 Dec 2021 08:44:24: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:44:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:44:25: #2 number of paired peaks: 1227 INFO @ Sat, 11 Dec 2021 08:44:25: start model_add_line... INFO @ Sat, 11 Dec 2021 08:44:25: 12000000 INFO @ Sat, 11 Dec 2021 08:44:25: start X-correlation... INFO @ Sat, 11 Dec 2021 08:44:25: end of X-cor INFO @ Sat, 11 Dec 2021 08:44:25: #2 finished! INFO @ Sat, 11 Dec 2021 08:44:25: #2 predicted fragment length is 157 bps INFO @ Sat, 11 Dec 2021 08:44:25: #2 alternative fragment length(s) may be 4,157 bps INFO @ Sat, 11 Dec 2021 08:44:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.05_model.r INFO @ Sat, 11 Dec 2021 08:44:25: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:44:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:44:28: 7000000 INFO @ Sat, 11 Dec 2021 08:44:31: 13000000 INFO @ Sat, 11 Dec 2021 08:44:34: 8000000 INFO @ Sat, 11 Dec 2021 08:44:36: 14000000 INFO @ Sat, 11 Dec 2021 08:44:40: 9000000 INFO @ Sat, 11 Dec 2021 08:44:42: 15000000 INFO @ Sat, 11 Dec 2021 08:44:45: 10000000 INFO @ Sat, 11 Dec 2021 08:44:48: 16000000 INFO @ Sat, 11 Dec 2021 08:44:51: 11000000 INFO @ Sat, 11 Dec 2021 08:44:53: 17000000 INFO @ Sat, 11 Dec 2021 08:44:57: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:44:57: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:44:57: #1 total tags in treatment: 17673293 INFO @ Sat, 11 Dec 2021 08:44:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:44:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:44:57: 12000000 INFO @ Sat, 11 Dec 2021 08:44:58: #1 tags after filtering in treatment: 17673227 INFO @ Sat, 11 Dec 2021 08:44:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:44:58: #1 finished! INFO @ Sat, 11 Dec 2021 08:44:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:44:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:44:59: #2 number of paired peaks: 1227 INFO @ Sat, 11 Dec 2021 08:44:59: start model_add_line... INFO @ Sat, 11 Dec 2021 08:44:59: start X-correlation... INFO @ Sat, 11 Dec 2021 08:44:59: end of X-cor INFO @ Sat, 11 Dec 2021 08:44:59: #2 finished! INFO @ Sat, 11 Dec 2021 08:44:59: #2 predicted fragment length is 157 bps INFO @ Sat, 11 Dec 2021 08:44:59: #2 alternative fragment length(s) may be 4,157 bps INFO @ Sat, 11 Dec 2021 08:44:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.10_model.r INFO @ Sat, 11 Dec 2021 08:44:59: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:44:59: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:45:01: #3 Call peaks for each chromosome... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 08:45:03: 13000000 INFO @ Sat, 11 Dec 2021 08:45:09: 14000000 INFO @ Sat, 11 Dec 2021 08:45:15: 15000000 INFO @ Sat, 11 Dec 2021 08:45:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.05_peaks.xls INFO @ Sat, 11 Dec 2021 08:45:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:45:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.05_summits.bed INFO @ Sat, 11 Dec 2021 08:45:18: Done! pass1 - making usageList (662 chroms): 2 millis pass2 - checking and writing primary data (4890 records, 4 fields): 24 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:45:21: 16000000 INFO @ Sat, 11 Dec 2021 08:45:27: 17000000 INFO @ Sat, 11 Dec 2021 08:45:31: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:45:31: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:45:31: #1 total tags in treatment: 17673293 INFO @ Sat, 11 Dec 2021 08:45:31: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:45:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:45:32: #1 tags after filtering in treatment: 17673227 INFO @ Sat, 11 Dec 2021 08:45:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:45:32: #1 finished! INFO @ Sat, 11 Dec 2021 08:45:32: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:45:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:45:33: #2 number of paired peaks: 1227 INFO @ Sat, 11 Dec 2021 08:45:33: start model_add_line... INFO @ Sat, 11 Dec 2021 08:45:33: start X-correlation... INFO @ Sat, 11 Dec 2021 08:45:33: end of X-cor INFO @ Sat, 11 Dec 2021 08:45:33: #2 finished! INFO @ Sat, 11 Dec 2021 08:45:33: #2 predicted fragment length is 157 bps INFO @ Sat, 11 Dec 2021 08:45:33: #2 alternative fragment length(s) may be 4,157 bps INFO @ Sat, 11 Dec 2021 08:45:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.20_model.r INFO @ Sat, 11 Dec 2021 08:45:33: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:45:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:45:35: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 08:45:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.10_peaks.xls INFO @ Sat, 11 Dec 2021 08:45:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:45:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.10_summits.bed INFO @ Sat, 11 Dec 2021 08:45:53: Done! pass1 - making usageList (594 chroms): 2 millis pass2 - checking and writing primary data (3522 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:46:10: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:46:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.20_peaks.xls INFO @ Sat, 11 Dec 2021 08:46:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:46:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720909/SRX9720909.20_summits.bed INFO @ Sat, 11 Dec 2021 08:46:28: Done! pass1 - making usageList (515 chroms): 1 millis pass2 - checking and writing primary data (2442 records, 4 fields): 15 millis CompletedMACS2peakCalling