Job ID = 14170778 SRX = SRX9720905 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:16 26709476 reads; of these: 26709476 (100.00%) were unpaired; of these: 1165577 (4.36%) aligned 0 times 17561276 (65.75%) aligned exactly 1 time 7982623 (29.89%) aligned >1 times 95.64% overall alignment rate Time searching: 00:09:16 Overall time: 00:09:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4022533 / 25543899 = 0.1575 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:52:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:52:56: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:52:56: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:53:05: 1000000 INFO @ Sat, 11 Dec 2021 08:53:14: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:53:23: 3000000 INFO @ Sat, 11 Dec 2021 08:53:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:53:26: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:53:26: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:53:33: 4000000 INFO @ Sat, 11 Dec 2021 08:53:35: 1000000 INFO @ Sat, 11 Dec 2021 08:53:42: 5000000 INFO @ Sat, 11 Dec 2021 08:53:45: 2000000 INFO @ Sat, 11 Dec 2021 08:53:51: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:53:54: 3000000 INFO @ Sat, 11 Dec 2021 08:53:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:53:56: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:53:56: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:54:01: 7000000 INFO @ Sat, 11 Dec 2021 08:54:04: 4000000 INFO @ Sat, 11 Dec 2021 08:54:09: 1000000 INFO @ Sat, 11 Dec 2021 08:54:11: 8000000 INFO @ Sat, 11 Dec 2021 08:54:14: 5000000 INFO @ Sat, 11 Dec 2021 08:54:22: 9000000 INFO @ Sat, 11 Dec 2021 08:54:22: 2000000 INFO @ Sat, 11 Dec 2021 08:54:25: 6000000 INFO @ Sat, 11 Dec 2021 08:54:32: 10000000 INFO @ Sat, 11 Dec 2021 08:54:34: 3000000 INFO @ Sat, 11 Dec 2021 08:54:35: 7000000 INFO @ Sat, 11 Dec 2021 08:54:41: 11000000 INFO @ Sat, 11 Dec 2021 08:54:44: 8000000 INFO @ Sat, 11 Dec 2021 08:54:47: 4000000 INFO @ Sat, 11 Dec 2021 08:54:51: 12000000 INFO @ Sat, 11 Dec 2021 08:54:54: 9000000 INFO @ Sat, 11 Dec 2021 08:55:00: 5000000 INFO @ Sat, 11 Dec 2021 08:55:01: 13000000 INFO @ Sat, 11 Dec 2021 08:55:04: 10000000 INFO @ Sat, 11 Dec 2021 08:55:11: 14000000 INFO @ Sat, 11 Dec 2021 08:55:12: 6000000 INFO @ Sat, 11 Dec 2021 08:55:14: 11000000 INFO @ Sat, 11 Dec 2021 08:55:21: 15000000 INFO @ Sat, 11 Dec 2021 08:55:23: 12000000 INFO @ Sat, 11 Dec 2021 08:55:25: 7000000 INFO @ Sat, 11 Dec 2021 08:55:31: 16000000 INFO @ Sat, 11 Dec 2021 08:55:33: 13000000 INFO @ Sat, 11 Dec 2021 08:55:37: 8000000 INFO @ Sat, 11 Dec 2021 08:55:41: 17000000 INFO @ Sat, 11 Dec 2021 08:55:43: 14000000 INFO @ Sat, 11 Dec 2021 08:55:48: 9000000 INFO @ Sat, 11 Dec 2021 08:55:52: 18000000 INFO @ Sat, 11 Dec 2021 08:55:53: 15000000 INFO @ Sat, 11 Dec 2021 08:55:59: 10000000 INFO @ Sat, 11 Dec 2021 08:56:02: 19000000 INFO @ Sat, 11 Dec 2021 08:56:03: 16000000 INFO @ Sat, 11 Dec 2021 08:56:10: 11000000 INFO @ Sat, 11 Dec 2021 08:56:12: 20000000 INFO @ Sat, 11 Dec 2021 08:56:13: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 08:56:20: 12000000 INFO @ Sat, 11 Dec 2021 08:56:22: 21000000 INFO @ Sat, 11 Dec 2021 08:56:23: 18000000 INFO @ Sat, 11 Dec 2021 08:56:27: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:56:27: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:56:27: #1 total tags in treatment: 21521366 INFO @ Sat, 11 Dec 2021 08:56:27: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:56:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:56:28: #1 tags after filtering in treatment: 21521301 INFO @ Sat, 11 Dec 2021 08:56:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:56:28: #1 finished! INFO @ Sat, 11 Dec 2021 08:56:28: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:56:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:56:30: #2 number of paired peaks: 1024 INFO @ Sat, 11 Dec 2021 08:56:30: start model_add_line... INFO @ Sat, 11 Dec 2021 08:56:30: start X-correlation... INFO @ Sat, 11 Dec 2021 08:56:30: end of X-cor INFO @ Sat, 11 Dec 2021 08:56:30: #2 finished! INFO @ Sat, 11 Dec 2021 08:56:30: #2 predicted fragment length is 1 bps INFO @ Sat, 11 Dec 2021 08:56:30: #2 alternative fragment length(s) may be 1,37,44,554,584 bps INFO @ Sat, 11 Dec 2021 08:56:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.05_model.r WARNING @ Sat, 11 Dec 2021 08:56:30: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 08:56:30: #2 You may need to consider one of the other alternative d(s): 1,37,44,554,584 WARNING @ Sat, 11 Dec 2021 08:56:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 08:56:30: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:56:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:56:31: 13000000 INFO @ Sat, 11 Dec 2021 08:56:32: 19000000 INFO @ Sat, 11 Dec 2021 08:56:41: 20000000 INFO @ Sat, 11 Dec 2021 08:56:42: 14000000 INFO @ Sat, 11 Dec 2021 08:56:49: 21000000 INFO @ Sat, 11 Dec 2021 08:56:53: 15000000 INFO @ Sat, 11 Dec 2021 08:56:54: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:56:54: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:56:54: #1 total tags in treatment: 21521366 INFO @ Sat, 11 Dec 2021 08:56:54: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:56:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:56:55: #1 tags after filtering in treatment: 21521301 INFO @ Sat, 11 Dec 2021 08:56:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:56:55: #1 finished! INFO @ Sat, 11 Dec 2021 08:56:55: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:56:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:56:57: #2 number of paired peaks: 1024 INFO @ Sat, 11 Dec 2021 08:56:57: start model_add_line... INFO @ Sat, 11 Dec 2021 08:56:57: start X-correlation... INFO @ Sat, 11 Dec 2021 08:56:57: end of X-cor INFO @ Sat, 11 Dec 2021 08:56:57: #2 finished! INFO @ Sat, 11 Dec 2021 08:56:57: #2 predicted fragment length is 1 bps INFO @ Sat, 11 Dec 2021 08:56:57: #2 alternative fragment length(s) may be 1,37,44,554,584 bps INFO @ Sat, 11 Dec 2021 08:56:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.10_model.r WARNING @ Sat, 11 Dec 2021 08:56:57: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 08:56:57: #2 You may need to consider one of the other alternative d(s): 1,37,44,554,584 WARNING @ Sat, 11 Dec 2021 08:56:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 08:56:57: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:56:57: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:57:03: 16000000 INFO @ Sat, 11 Dec 2021 08:57:14: 17000000 INFO @ Sat, 11 Dec 2021 08:57:23: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:57:25: 18000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 08:57:36: 19000000 INFO @ Sat, 11 Dec 2021 08:57:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.05_peaks.xls INFO @ Sat, 11 Dec 2021 08:57:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:57:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.05_summits.bed INFO @ Sat, 11 Dec 2021 08:57:47: Done! INFO @ Sat, 11 Dec 2021 08:57:47: 20000000 pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:57:49: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:57:58: 21000000 INFO @ Sat, 11 Dec 2021 08:58:04: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:58:04: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:58:04: #1 total tags in treatment: 21521366 INFO @ Sat, 11 Dec 2021 08:58:04: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:58:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:58:05: #1 tags after filtering in treatment: 21521301 INFO @ Sat, 11 Dec 2021 08:58:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:58:05: #1 finished! INFO @ Sat, 11 Dec 2021 08:58:05: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:58:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:58:07: #2 number of paired peaks: 1024 INFO @ Sat, 11 Dec 2021 08:58:07: start model_add_line... INFO @ Sat, 11 Dec 2021 08:58:07: start X-correlation... INFO @ Sat, 11 Dec 2021 08:58:07: end of X-cor INFO @ Sat, 11 Dec 2021 08:58:07: #2 finished! INFO @ Sat, 11 Dec 2021 08:58:07: #2 predicted fragment length is 1 bps INFO @ Sat, 11 Dec 2021 08:58:07: #2 alternative fragment length(s) may be 1,37,44,554,584 bps INFO @ Sat, 11 Dec 2021 08:58:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.20_model.r WARNING @ Sat, 11 Dec 2021 08:58:07: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 08:58:07: #2 You may need to consider one of the other alternative d(s): 1,37,44,554,584 WARNING @ Sat, 11 Dec 2021 08:58:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 08:58:07: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:58:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:58:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.10_peaks.xls INFO @ Sat, 11 Dec 2021 08:58:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:58:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.10_summits.bed INFO @ Sat, 11 Dec 2021 08:58:13: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:58:59: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:59:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.20_peaks.xls INFO @ Sat, 11 Dec 2021 08:59:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:59:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720905/SRX9720905.20_summits.bed INFO @ Sat, 11 Dec 2021 08:59:23: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling