Job ID = 14170769 SRX = SRX9720904 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:29 19657290 reads; of these: 19657290 (100.00%) were unpaired; of these: 899358 (4.58%) aligned 0 times 12940029 (65.83%) aligned exactly 1 time 5817903 (29.60%) aligned >1 times 95.42% overall alignment rate Time searching: 00:06:29 Overall time: 00:06:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3876687 / 18757932 = 0.2067 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:40:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:40:29: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:40:29: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:40:35: 1000000 INFO @ Sat, 11 Dec 2021 08:40:40: 2000000 INFO @ Sat, 11 Dec 2021 08:40:45: 3000000 INFO @ Sat, 11 Dec 2021 08:40:51: 4000000 INFO @ Sat, 11 Dec 2021 08:40:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:40:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:40:59: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:40:59: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:41:02: 6000000 INFO @ Sat, 11 Dec 2021 08:41:06: 1000000 INFO @ Sat, 11 Dec 2021 08:41:08: 7000000 INFO @ Sat, 11 Dec 2021 08:41:12: 2000000 INFO @ Sat, 11 Dec 2021 08:41:15: 8000000 INFO @ Sat, 11 Dec 2021 08:41:19: 3000000 INFO @ Sat, 11 Dec 2021 08:41:21: 9000000 INFO @ Sat, 11 Dec 2021 08:41:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:41:28: 10000000 INFO @ Sat, 11 Dec 2021 08:41:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:41:29: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:41:29: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:41:32: 5000000 INFO @ Sat, 11 Dec 2021 08:41:35: 11000000 INFO @ Sat, 11 Dec 2021 08:41:37: 1000000 INFO @ Sat, 11 Dec 2021 08:41:38: 6000000 INFO @ Sat, 11 Dec 2021 08:41:41: 12000000 INFO @ Sat, 11 Dec 2021 08:41:44: 2000000 INFO @ Sat, 11 Dec 2021 08:41:45: 7000000 INFO @ Sat, 11 Dec 2021 08:41:49: 13000000 INFO @ Sat, 11 Dec 2021 08:41:51: 3000000 INFO @ Sat, 11 Dec 2021 08:41:52: 8000000 INFO @ Sat, 11 Dec 2021 08:41:55: 14000000 INFO @ Sat, 11 Dec 2021 08:41:59: 4000000 INFO @ Sat, 11 Dec 2021 08:41:59: 9000000 INFO @ Sat, 11 Dec 2021 08:42:02: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:42:02: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:42:02: #1 total tags in treatment: 14881245 INFO @ Sat, 11 Dec 2021 08:42:02: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:42:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:42:02: #1 tags after filtering in treatment: 14881174 INFO @ Sat, 11 Dec 2021 08:42:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:42:02: #1 finished! INFO @ Sat, 11 Dec 2021 08:42:02: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:42:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:42:03: #2 number of paired peaks: 1248 INFO @ Sat, 11 Dec 2021 08:42:03: start model_add_line... INFO @ Sat, 11 Dec 2021 08:42:03: start X-correlation... INFO @ Sat, 11 Dec 2021 08:42:03: end of X-cor INFO @ Sat, 11 Dec 2021 08:42:03: #2 finished! INFO @ Sat, 11 Dec 2021 08:42:03: #2 predicted fragment length is 130 bps INFO @ Sat, 11 Dec 2021 08:42:03: #2 alternative fragment length(s) may be 4,130 bps INFO @ Sat, 11 Dec 2021 08:42:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.05_model.r INFO @ Sat, 11 Dec 2021 08:42:03: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:42:03: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:42:05: 10000000 INFO @ Sat, 11 Dec 2021 08:42:06: 5000000 INFO @ Sat, 11 Dec 2021 08:42:12: 11000000 INFO @ Sat, 11 Dec 2021 08:42:13: 6000000 INFO @ Sat, 11 Dec 2021 08:42:19: 12000000 INFO @ Sat, 11 Dec 2021 08:42:21: 7000000 INFO @ Sat, 11 Dec 2021 08:42:26: 13000000 INFO @ Sat, 11 Dec 2021 08:42:28: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 08:42:32: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:42:33: 14000000 INFO @ Sat, 11 Dec 2021 08:42:35: 9000000 INFO @ Sat, 11 Dec 2021 08:42:39: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:42:39: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:42:39: #1 total tags in treatment: 14881245 INFO @ Sat, 11 Dec 2021 08:42:39: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:42:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:42:39: #1 tags after filtering in treatment: 14881174 INFO @ Sat, 11 Dec 2021 08:42:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:42:39: #1 finished! INFO @ Sat, 11 Dec 2021 08:42:39: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:42:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:42:41: #2 number of paired peaks: 1248 INFO @ Sat, 11 Dec 2021 08:42:41: start model_add_line... INFO @ Sat, 11 Dec 2021 08:42:41: start X-correlation... INFO @ Sat, 11 Dec 2021 08:42:41: end of X-cor INFO @ Sat, 11 Dec 2021 08:42:41: #2 finished! INFO @ Sat, 11 Dec 2021 08:42:41: #2 predicted fragment length is 130 bps INFO @ Sat, 11 Dec 2021 08:42:41: #2 alternative fragment length(s) may be 4,130 bps INFO @ Sat, 11 Dec 2021 08:42:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.10_model.r INFO @ Sat, 11 Dec 2021 08:42:41: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:42:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:42:42: 10000000 INFO @ Sat, 11 Dec 2021 08:42:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.05_peaks.xls INFO @ Sat, 11 Dec 2021 08:42:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:42:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.05_summits.bed INFO @ Sat, 11 Dec 2021 08:42:46: Done! pass1 - making usageList (645 chroms): 2 millis pass2 - checking and writing primary data (3979 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:42:49: 11000000 INFO @ Sat, 11 Dec 2021 08:42:55: 12000000 INFO @ Sat, 11 Dec 2021 08:43:02: 13000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 08:43:09: 14000000 INFO @ Sat, 11 Dec 2021 08:43:10: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:43:15: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:43:15: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:43:15: #1 total tags in treatment: 14881245 INFO @ Sat, 11 Dec 2021 08:43:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:43:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:43:15: #1 tags after filtering in treatment: 14881174 INFO @ Sat, 11 Dec 2021 08:43:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:43:15: #1 finished! INFO @ Sat, 11 Dec 2021 08:43:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:43:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:43:16: #2 number of paired peaks: 1248 INFO @ Sat, 11 Dec 2021 08:43:16: start model_add_line... INFO @ Sat, 11 Dec 2021 08:43:16: start X-correlation... INFO @ Sat, 11 Dec 2021 08:43:17: end of X-cor INFO @ Sat, 11 Dec 2021 08:43:17: #2 finished! INFO @ Sat, 11 Dec 2021 08:43:17: #2 predicted fragment length is 130 bps INFO @ Sat, 11 Dec 2021 08:43:17: #2 alternative fragment length(s) may be 4,130 bps INFO @ Sat, 11 Dec 2021 08:43:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.20_model.r INFO @ Sat, 11 Dec 2021 08:43:17: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:43:17: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:43:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.10_peaks.xls INFO @ Sat, 11 Dec 2021 08:43:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:43:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.10_summits.bed INFO @ Sat, 11 Dec 2021 08:43:24: Done! pass1 - making usageList (548 chroms): 1 millis pass2 - checking and writing primary data (2732 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:43:47: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:44:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.20_peaks.xls INFO @ Sat, 11 Dec 2021 08:44:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:44:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720904/SRX9720904.20_summits.bed INFO @ Sat, 11 Dec 2021 08:44:01: Done! pass1 - making usageList (446 chroms): 1 millis pass2 - checking and writing primary data (1860 records, 4 fields): 14 millis CompletedMACS2peakCalling