Job ID = 14171004 SRX = SRX9720896 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:53 27860188 reads; of these: 27860188 (100.00%) were unpaired; of these: 1071875 (3.85%) aligned 0 times 18383128 (65.98%) aligned exactly 1 time 8405185 (30.17%) aligned >1 times 96.15% overall alignment rate Time searching: 00:09:53 Overall time: 00:09:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7005623 / 26788313 = 0.2615 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:26:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:26:41: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:26:41: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:26:51: 1000000 INFO @ Sat, 11 Dec 2021 09:27:01: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:27:11: 3000000 INFO @ Sat, 11 Dec 2021 09:27:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:27:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:27:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:27:21: 4000000 INFO @ Sat, 11 Dec 2021 09:27:21: 1000000 INFO @ Sat, 11 Dec 2021 09:27:31: 5000000 INFO @ Sat, 11 Dec 2021 09:27:31: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:27:41: 3000000 INFO @ Sat, 11 Dec 2021 09:27:41: 6000000 INFO @ Sat, 11 Dec 2021 09:27:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:27:41: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:27:41: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:27:50: 1000000 INFO @ Sat, 11 Dec 2021 09:27:51: 4000000 INFO @ Sat, 11 Dec 2021 09:27:52: 7000000 INFO @ Sat, 11 Dec 2021 09:27:59: 2000000 INFO @ Sat, 11 Dec 2021 09:28:02: 5000000 INFO @ Sat, 11 Dec 2021 09:28:02: 8000000 INFO @ Sat, 11 Dec 2021 09:28:08: 3000000 INFO @ Sat, 11 Dec 2021 09:28:11: 6000000 INFO @ Sat, 11 Dec 2021 09:28:13: 9000000 INFO @ Sat, 11 Dec 2021 09:28:17: 4000000 INFO @ Sat, 11 Dec 2021 09:28:21: 7000000 INFO @ Sat, 11 Dec 2021 09:28:24: 10000000 INFO @ Sat, 11 Dec 2021 09:28:26: 5000000 INFO @ Sat, 11 Dec 2021 09:28:32: 8000000 INFO @ Sat, 11 Dec 2021 09:28:34: 11000000 INFO @ Sat, 11 Dec 2021 09:28:35: 6000000 INFO @ Sat, 11 Dec 2021 09:28:42: 9000000 INFO @ Sat, 11 Dec 2021 09:28:44: 7000000 INFO @ Sat, 11 Dec 2021 09:28:45: 12000000 INFO @ Sat, 11 Dec 2021 09:28:52: 10000000 INFO @ Sat, 11 Dec 2021 09:28:53: 8000000 INFO @ Sat, 11 Dec 2021 09:28:55: 13000000 INFO @ Sat, 11 Dec 2021 09:29:02: 11000000 INFO @ Sat, 11 Dec 2021 09:29:02: 9000000 INFO @ Sat, 11 Dec 2021 09:29:05: 14000000 INFO @ Sat, 11 Dec 2021 09:29:11: 10000000 INFO @ Sat, 11 Dec 2021 09:29:12: 12000000 INFO @ Sat, 11 Dec 2021 09:29:16: 15000000 INFO @ Sat, 11 Dec 2021 09:29:20: 11000000 INFO @ Sat, 11 Dec 2021 09:29:22: 13000000 INFO @ Sat, 11 Dec 2021 09:29:27: 16000000 INFO @ Sat, 11 Dec 2021 09:29:28: 12000000 INFO @ Sat, 11 Dec 2021 09:29:33: 14000000 INFO @ Sat, 11 Dec 2021 09:29:37: 13000000 INFO @ Sat, 11 Dec 2021 09:29:38: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:29:43: 15000000 INFO @ Sat, 11 Dec 2021 09:29:46: 14000000 INFO @ Sat, 11 Dec 2021 09:29:48: 18000000 INFO @ Sat, 11 Dec 2021 09:29:54: 16000000 INFO @ Sat, 11 Dec 2021 09:29:55: 15000000 INFO @ Sat, 11 Dec 2021 09:29:59: 19000000 INFO @ Sat, 11 Dec 2021 09:30:03: 16000000 INFO @ Sat, 11 Dec 2021 09:30:05: 17000000 INFO @ Sat, 11 Dec 2021 09:30:08: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:30:08: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:30:08: #1 total tags in treatment: 19782690 INFO @ Sat, 11 Dec 2021 09:30:08: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:30:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:30:09: #1 tags after filtering in treatment: 19782623 INFO @ Sat, 11 Dec 2021 09:30:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:30:09: #1 finished! INFO @ Sat, 11 Dec 2021 09:30:09: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:30:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:30:10: #2 number of paired peaks: 1040 INFO @ Sat, 11 Dec 2021 09:30:10: start model_add_line... INFO @ Sat, 11 Dec 2021 09:30:11: start X-correlation... INFO @ Sat, 11 Dec 2021 09:30:11: end of X-cor INFO @ Sat, 11 Dec 2021 09:30:11: #2 finished! INFO @ Sat, 11 Dec 2021 09:30:11: #2 predicted fragment length is 1 bps INFO @ Sat, 11 Dec 2021 09:30:11: #2 alternative fragment length(s) may be 1,43 bps INFO @ Sat, 11 Dec 2021 09:30:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.05_model.r WARNING @ Sat, 11 Dec 2021 09:30:11: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 09:30:11: #2 You may need to consider one of the other alternative d(s): 1,43 WARNING @ Sat, 11 Dec 2021 09:30:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 09:30:11: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:30:11: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:30:13: 17000000 INFO @ Sat, 11 Dec 2021 09:30:15: 18000000 INFO @ Sat, 11 Dec 2021 09:30:21: 18000000 INFO @ Sat, 11 Dec 2021 09:30:26: 19000000 INFO @ Sat, 11 Dec 2021 09:30:30: 19000000 INFO @ Sat, 11 Dec 2021 09:30:34: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:30:34: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:30:34: #1 total tags in treatment: 19782690 INFO @ Sat, 11 Dec 2021 09:30:34: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:30:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:30:35: #1 tags after filtering in treatment: 19782623 INFO @ Sat, 11 Dec 2021 09:30:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:30:35: #1 finished! INFO @ Sat, 11 Dec 2021 09:30:35: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:30:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:30:37: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:30:37: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:30:37: #1 total tags in treatment: 19782690 INFO @ Sat, 11 Dec 2021 09:30:37: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:30:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:30:37: #2 number of paired peaks: 1040 INFO @ Sat, 11 Dec 2021 09:30:37: start model_add_line... INFO @ Sat, 11 Dec 2021 09:30:37: start X-correlation... INFO @ Sat, 11 Dec 2021 09:30:37: end of X-cor INFO @ Sat, 11 Dec 2021 09:30:37: #2 finished! INFO @ Sat, 11 Dec 2021 09:30:37: #2 predicted fragment length is 1 bps INFO @ Sat, 11 Dec 2021 09:30:37: #2 alternative fragment length(s) may be 1,43 bps INFO @ Sat, 11 Dec 2021 09:30:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.10_model.r WARNING @ Sat, 11 Dec 2021 09:30:37: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 09:30:37: #2 You may need to consider one of the other alternative d(s): 1,43 WARNING @ Sat, 11 Dec 2021 09:30:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 09:30:37: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:30:37: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:30:38: #1 tags after filtering in treatment: 19782623 INFO @ Sat, 11 Dec 2021 09:30:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:30:38: #1 finished! INFO @ Sat, 11 Dec 2021 09:30:38: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:30:38: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 09:30:40: #2 number of paired peaks: 1040 INFO @ Sat, 11 Dec 2021 09:30:40: start model_add_line... INFO @ Sat, 11 Dec 2021 09:30:40: start X-correlation... INFO @ Sat, 11 Dec 2021 09:30:40: end of X-cor INFO @ Sat, 11 Dec 2021 09:30:40: #2 finished! INFO @ Sat, 11 Dec 2021 09:30:40: #2 predicted fragment length is 1 bps INFO @ Sat, 11 Dec 2021 09:30:40: #2 alternative fragment length(s) may be 1,43 bps INFO @ Sat, 11 Dec 2021 09:30:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.20_model.r WARNING @ Sat, 11 Dec 2021 09:30:40: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 09:30:40: #2 You may need to consider one of the other alternative d(s): 1,43 WARNING @ Sat, 11 Dec 2021 09:30:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 09:30:40: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:30:40: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:30:57: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:31:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.05_peaks.xls INFO @ Sat, 11 Dec 2021 09:31:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:31:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.05_summits.bed INFO @ Sat, 11 Dec 2021 09:31:18: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:31:24: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:31:26: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:31:45: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.10_peaks.xls INFO @ Sat, 11 Dec 2021 09:31:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:31:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.10_summits.bed INFO @ Sat, 11 Dec 2021 09:31:45: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:31:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.20_peaks.xls INFO @ Sat, 11 Dec 2021 09:31:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:31:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9720896/SRX9720896.20_summits.bed INFO @ Sat, 11 Dec 2021 09:31:47: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling