Job ID = 14167394 SRX = SRX9717947 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:02 44430266 reads; of these: 44430266 (100.00%) were unpaired; of these: 7745368 (17.43%) aligned 0 times 27802493 (62.58%) aligned exactly 1 time 8882405 (19.99%) aligned >1 times 82.57% overall alignment rate Time searching: 00:13:02 Overall time: 00:13:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15917289 / 36684898 = 0.4339 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:26:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:26:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:26:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:26:48: 1000000 INFO @ Fri, 10 Dec 2021 12:26:53: 2000000 INFO @ Fri, 10 Dec 2021 12:26:59: 3000000 INFO @ Fri, 10 Dec 2021 12:27:04: 4000000 INFO @ Fri, 10 Dec 2021 12:27:09: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:27:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:27:12: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:27:12: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:27:15: 6000000 INFO @ Fri, 10 Dec 2021 12:27:19: 1000000 INFO @ Fri, 10 Dec 2021 12:27:22: 7000000 INFO @ Fri, 10 Dec 2021 12:27:26: 2000000 INFO @ Fri, 10 Dec 2021 12:27:28: 8000000 INFO @ Fri, 10 Dec 2021 12:27:32: 3000000 INFO @ Fri, 10 Dec 2021 12:27:35: 9000000 INFO @ Fri, 10 Dec 2021 12:27:39: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:27:41: 10000000 INFO @ Fri, 10 Dec 2021 12:27:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:27:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:27:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:27:45: 5000000 INFO @ Fri, 10 Dec 2021 12:27:48: 11000000 INFO @ Fri, 10 Dec 2021 12:27:50: 1000000 INFO @ Fri, 10 Dec 2021 12:27:52: 6000000 INFO @ Fri, 10 Dec 2021 12:27:55: 12000000 INFO @ Fri, 10 Dec 2021 12:27:57: 2000000 INFO @ Fri, 10 Dec 2021 12:27:59: 7000000 INFO @ Fri, 10 Dec 2021 12:28:01: 13000000 INFO @ Fri, 10 Dec 2021 12:28:05: 3000000 INFO @ Fri, 10 Dec 2021 12:28:05: 8000000 INFO @ Fri, 10 Dec 2021 12:28:08: 14000000 INFO @ Fri, 10 Dec 2021 12:28:12: 9000000 INFO @ Fri, 10 Dec 2021 12:28:12: 4000000 INFO @ Fri, 10 Dec 2021 12:28:14: 15000000 INFO @ Fri, 10 Dec 2021 12:28:19: 10000000 INFO @ Fri, 10 Dec 2021 12:28:19: 5000000 INFO @ Fri, 10 Dec 2021 12:28:21: 16000000 INFO @ Fri, 10 Dec 2021 12:28:25: 11000000 INFO @ Fri, 10 Dec 2021 12:28:27: 6000000 INFO @ Fri, 10 Dec 2021 12:28:28: 17000000 INFO @ Fri, 10 Dec 2021 12:28:32: 12000000 INFO @ Fri, 10 Dec 2021 12:28:34: 7000000 INFO @ Fri, 10 Dec 2021 12:28:35: 18000000 INFO @ Fri, 10 Dec 2021 12:28:39: 13000000 INFO @ Fri, 10 Dec 2021 12:28:41: 19000000 INFO @ Fri, 10 Dec 2021 12:28:42: 8000000 INFO @ Fri, 10 Dec 2021 12:28:45: 14000000 INFO @ Fri, 10 Dec 2021 12:28:48: 20000000 INFO @ Fri, 10 Dec 2021 12:28:49: 9000000 INFO @ Fri, 10 Dec 2021 12:28:52: 15000000 INFO @ Fri, 10 Dec 2021 12:28:53: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:28:53: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:28:53: #1 total tags in treatment: 20767609 INFO @ Fri, 10 Dec 2021 12:28:53: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:28:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:28:54: #1 tags after filtering in treatment: 20767608 INFO @ Fri, 10 Dec 2021 12:28:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:28:54: #1 finished! INFO @ Fri, 10 Dec 2021 12:28:54: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:28:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:28:55: #2 number of paired peaks: 84 WARNING @ Fri, 10 Dec 2021 12:28:55: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:28:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:28:56: 10000000 INFO @ Fri, 10 Dec 2021 12:28:58: 16000000 INFO @ Fri, 10 Dec 2021 12:29:04: 11000000 INFO @ Fri, 10 Dec 2021 12:29:05: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:29:11: 12000000 INFO @ Fri, 10 Dec 2021 12:29:12: 18000000 INFO @ Fri, 10 Dec 2021 12:29:18: 19000000 INFO @ Fri, 10 Dec 2021 12:29:18: 13000000 INFO @ Fri, 10 Dec 2021 12:29:25: 20000000 INFO @ Fri, 10 Dec 2021 12:29:26: 14000000 INFO @ Fri, 10 Dec 2021 12:29:30: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:29:30: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:29:30: #1 total tags in treatment: 20767609 INFO @ Fri, 10 Dec 2021 12:29:30: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:29:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:29:31: #1 tags after filtering in treatment: 20767608 INFO @ Fri, 10 Dec 2021 12:29:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:29:31: #1 finished! INFO @ Fri, 10 Dec 2021 12:29:31: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:29:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:29:32: #2 number of paired peaks: 84 WARNING @ Fri, 10 Dec 2021 12:29:32: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:29:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:29:33: 15000000 INFO @ Fri, 10 Dec 2021 12:29:40: 16000000 INFO @ Fri, 10 Dec 2021 12:29:46: 17000000 INFO @ Fri, 10 Dec 2021 12:29:53: 18000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 12:30:00: 19000000 INFO @ Fri, 10 Dec 2021 12:30:06: 20000000 INFO @ Fri, 10 Dec 2021 12:30:12: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:30:12: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:30:12: #1 total tags in treatment: 20767609 INFO @ Fri, 10 Dec 2021 12:30:12: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:30:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:30:12: #1 tags after filtering in treatment: 20767608 INFO @ Fri, 10 Dec 2021 12:30:12: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:30:12: #1 finished! INFO @ Fri, 10 Dec 2021 12:30:12: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:30:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:30:13: #2 number of paired peaks: 84 WARNING @ Fri, 10 Dec 2021 12:30:13: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:30:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX9717947/SRX9717947.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling