Job ID = 14171085 SRX = SRX9434470 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:34 26983538 reads; of these: 26983538 (100.00%) were unpaired; of these: 630491 (2.34%) aligned 0 times 22089510 (81.86%) aligned exactly 1 time 4263537 (15.80%) aligned >1 times 97.66% overall alignment rate Time searching: 00:06:34 Overall time: 00:06:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11369176 / 26353047 = 0.4314 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:40:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:40:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:40:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:40:26: 1000000 INFO @ Sat, 11 Dec 2021 09:40:32: 2000000 INFO @ Sat, 11 Dec 2021 09:40:38: 3000000 INFO @ Sat, 11 Dec 2021 09:40:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:40:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:40:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:40:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:40:50: 5000000 INFO @ Sat, 11 Dec 2021 09:40:56: 1000000 INFO @ Sat, 11 Dec 2021 09:40:57: 6000000 INFO @ Sat, 11 Dec 2021 09:41:03: 2000000 INFO @ Sat, 11 Dec 2021 09:41:03: 7000000 INFO @ Sat, 11 Dec 2021 09:41:09: 3000000 INFO @ Sat, 11 Dec 2021 09:41:10: 8000000 INFO @ Sat, 11 Dec 2021 09:41:16: 4000000 INFO @ Sat, 11 Dec 2021 09:41:17: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:41:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:41:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:41:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:41:22: 5000000 INFO @ Sat, 11 Dec 2021 09:41:23: 10000000 INFO @ Sat, 11 Dec 2021 09:41:27: 1000000 INFO @ Sat, 11 Dec 2021 09:41:30: 6000000 INFO @ Sat, 11 Dec 2021 09:41:30: 11000000 INFO @ Sat, 11 Dec 2021 09:41:34: 2000000 INFO @ Sat, 11 Dec 2021 09:41:36: 7000000 INFO @ Sat, 11 Dec 2021 09:41:38: 12000000 INFO @ Sat, 11 Dec 2021 09:41:40: 3000000 INFO @ Sat, 11 Dec 2021 09:41:43: 8000000 INFO @ Sat, 11 Dec 2021 09:41:45: 13000000 INFO @ Sat, 11 Dec 2021 09:41:47: 4000000 INFO @ Sat, 11 Dec 2021 09:41:50: 9000000 INFO @ Sat, 11 Dec 2021 09:41:51: 14000000 INFO @ Sat, 11 Dec 2021 09:41:53: 5000000 INFO @ Sat, 11 Dec 2021 09:41:57: 10000000 INFO @ Sat, 11 Dec 2021 09:41:59: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:41:59: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:41:59: #1 total tags in treatment: 14983871 INFO @ Sat, 11 Dec 2021 09:41:59: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:41:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:41:59: #1 tags after filtering in treatment: 14983733 INFO @ Sat, 11 Dec 2021 09:41:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:41:59: #1 finished! INFO @ Sat, 11 Dec 2021 09:41:59: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:41:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:42:00: 6000000 INFO @ Sat, 11 Dec 2021 09:42:02: #2 number of paired peaks: 13731 INFO @ Sat, 11 Dec 2021 09:42:02: start model_add_line... INFO @ Sat, 11 Dec 2021 09:42:02: start X-correlation... INFO @ Sat, 11 Dec 2021 09:42:02: end of X-cor INFO @ Sat, 11 Dec 2021 09:42:02: #2 finished! INFO @ Sat, 11 Dec 2021 09:42:02: #2 predicted fragment length is 111 bps INFO @ Sat, 11 Dec 2021 09:42:02: #2 alternative fragment length(s) may be 111 bps INFO @ Sat, 11 Dec 2021 09:42:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.05_model.r INFO @ Sat, 11 Dec 2021 09:42:02: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:42:02: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:42:03: 11000000 INFO @ Sat, 11 Dec 2021 09:42:07: 7000000 INFO @ Sat, 11 Dec 2021 09:42:10: 12000000 INFO @ Sat, 11 Dec 2021 09:42:13: 8000000 INFO @ Sat, 11 Dec 2021 09:42:17: 13000000 INFO @ Sat, 11 Dec 2021 09:42:19: 9000000 INFO @ Sat, 11 Dec 2021 09:42:23: 14000000 INFO @ Sat, 11 Dec 2021 09:42:25: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:42:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:42:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:42:30: #1 total tags in treatment: 14983871 INFO @ Sat, 11 Dec 2021 09:42:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:42:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:42:31: #1 tags after filtering in treatment: 14983733 INFO @ Sat, 11 Dec 2021 09:42:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:42:31: #1 finished! INFO @ Sat, 11 Dec 2021 09:42:31: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:42:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:42:32: 11000000 INFO @ Sat, 11 Dec 2021 09:42:33: #2 number of paired peaks: 13731 INFO @ Sat, 11 Dec 2021 09:42:33: start model_add_line... INFO @ Sat, 11 Dec 2021 09:42:33: start X-correlation... INFO @ Sat, 11 Dec 2021 09:42:34: end of X-cor INFO @ Sat, 11 Dec 2021 09:42:34: #2 finished! INFO @ Sat, 11 Dec 2021 09:42:34: #2 predicted fragment length is 111 bps INFO @ Sat, 11 Dec 2021 09:42:34: #2 alternative fragment length(s) may be 111 bps INFO @ Sat, 11 Dec 2021 09:42:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.10_model.r INFO @ Sat, 11 Dec 2021 09:42:34: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:42:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:42:39: 12000000 INFO @ Sat, 11 Dec 2021 09:42:40: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:42:45: 13000000 INFO @ Sat, 11 Dec 2021 09:42:51: 14000000 INFO @ Sat, 11 Dec 2021 09:42:57: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:42:57: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:42:57: #1 total tags in treatment: 14983871 INFO @ Sat, 11 Dec 2021 09:42:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:42:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 09:42:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.05_peaks.xls INFO @ Sat, 11 Dec 2021 09:42:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:42:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.05_summits.bed INFO @ Sat, 11 Dec 2021 09:42:58: Done! INFO @ Sat, 11 Dec 2021 09:42:58: #1 tags after filtering in treatment: 14983733 INFO @ Sat, 11 Dec 2021 09:42:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:42:58: #1 finished! INFO @ Sat, 11 Dec 2021 09:42:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:42:58: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (192 chroms): 3 millis pass2 - checking and writing primary data (11146 records, 4 fields): 27 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:43:00: #2 number of paired peaks: 13731 INFO @ Sat, 11 Dec 2021 09:43:00: start model_add_line... INFO @ Sat, 11 Dec 2021 09:43:01: start X-correlation... INFO @ Sat, 11 Dec 2021 09:43:01: end of X-cor INFO @ Sat, 11 Dec 2021 09:43:01: #2 finished! INFO @ Sat, 11 Dec 2021 09:43:01: #2 predicted fragment length is 111 bps INFO @ Sat, 11 Dec 2021 09:43:01: #2 alternative fragment length(s) may be 111 bps INFO @ Sat, 11 Dec 2021 09:43:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.20_model.r INFO @ Sat, 11 Dec 2021 09:43:01: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:43:01: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:43:11: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:43:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.10_peaks.xls INFO @ Sat, 11 Dec 2021 09:43:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:43:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.10_summits.bed INFO @ Sat, 11 Dec 2021 09:43:28: Done! pass1 - making usageList (123 chroms): 4 millis pass2 - checking and writing primary data (9585 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:43:38: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:43:55: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.20_peaks.xls INFO @ Sat, 11 Dec 2021 09:43:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:43:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9434470/SRX9434470.20_summits.bed INFO @ Sat, 11 Dec 2021 09:43:55: Done! pass1 - making usageList (71 chroms): 2 millis pass2 - checking and writing primary data (7766 records, 4 fields): 15 millis CompletedMACS2peakCalling