Job ID = 14171078 SRX = SRX9434469 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:05 24908941 reads; of these: 24908941 (100.00%) were unpaired; of these: 522050 (2.10%) aligned 0 times 20431437 (82.02%) aligned exactly 1 time 3955454 (15.88%) aligned >1 times 97.90% overall alignment rate Time searching: 00:05:05 Overall time: 00:05:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10102518 / 24386891 = 0.4143 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:34:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:34:36: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:34:36: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:34:41: 1000000 INFO @ Sat, 11 Dec 2021 09:34:46: 2000000 INFO @ Sat, 11 Dec 2021 09:34:52: 3000000 INFO @ Sat, 11 Dec 2021 09:34:57: 4000000 INFO @ Sat, 11 Dec 2021 09:35:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:35:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:35:06: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:35:06: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:35:08: 6000000 INFO @ Sat, 11 Dec 2021 09:35:12: 1000000 INFO @ Sat, 11 Dec 2021 09:35:14: 7000000 INFO @ Sat, 11 Dec 2021 09:35:18: 2000000 INFO @ Sat, 11 Dec 2021 09:35:20: 8000000 INFO @ Sat, 11 Dec 2021 09:35:24: 3000000 INFO @ Sat, 11 Dec 2021 09:35:26: 9000000 INFO @ Sat, 11 Dec 2021 09:35:30: 4000000 INFO @ Sat, 11 Dec 2021 09:35:32: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:35:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:35:36: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:35:36: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:35:36: 5000000 INFO @ Sat, 11 Dec 2021 09:35:38: 11000000 INFO @ Sat, 11 Dec 2021 09:35:42: 1000000 INFO @ Sat, 11 Dec 2021 09:35:42: 6000000 INFO @ Sat, 11 Dec 2021 09:35:44: 12000000 INFO @ Sat, 11 Dec 2021 09:35:47: 2000000 INFO @ Sat, 11 Dec 2021 09:35:48: 7000000 INFO @ Sat, 11 Dec 2021 09:35:50: 13000000 INFO @ Sat, 11 Dec 2021 09:35:53: 3000000 INFO @ Sat, 11 Dec 2021 09:35:53: 8000000 INFO @ Sat, 11 Dec 2021 09:35:55: 14000000 INFO @ Sat, 11 Dec 2021 09:35:57: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:35:57: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:35:57: #1 total tags in treatment: 14284373 INFO @ Sat, 11 Dec 2021 09:35:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:35:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:35:58: #1 tags after filtering in treatment: 14284226 INFO @ Sat, 11 Dec 2021 09:35:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:35:58: #1 finished! INFO @ Sat, 11 Dec 2021 09:35:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:35:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:35:59: 4000000 INFO @ Sat, 11 Dec 2021 09:35:59: 9000000 INFO @ Sat, 11 Dec 2021 09:36:00: #2 number of paired peaks: 13918 INFO @ Sat, 11 Dec 2021 09:36:00: start model_add_line... INFO @ Sat, 11 Dec 2021 09:36:00: start X-correlation... INFO @ Sat, 11 Dec 2021 09:36:00: end of X-cor INFO @ Sat, 11 Dec 2021 09:36:00: #2 finished! INFO @ Sat, 11 Dec 2021 09:36:00: #2 predicted fragment length is 131 bps INFO @ Sat, 11 Dec 2021 09:36:00: #2 alternative fragment length(s) may be 131 bps INFO @ Sat, 11 Dec 2021 09:36:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.05_model.r INFO @ Sat, 11 Dec 2021 09:36:00: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:36:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:36:05: 5000000 INFO @ Sat, 11 Dec 2021 09:36:05: 10000000 INFO @ Sat, 11 Dec 2021 09:36:11: 6000000 INFO @ Sat, 11 Dec 2021 09:36:11: 11000000 INFO @ Sat, 11 Dec 2021 09:36:16: 7000000 INFO @ Sat, 11 Dec 2021 09:36:17: 12000000 INFO @ Sat, 11 Dec 2021 09:36:22: 8000000 INFO @ Sat, 11 Dec 2021 09:36:23: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:36:28: 9000000 INFO @ Sat, 11 Dec 2021 09:36:29: 14000000 INFO @ Sat, 11 Dec 2021 09:36:31: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:36:31: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:36:31: #1 total tags in treatment: 14284373 INFO @ Sat, 11 Dec 2021 09:36:31: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:36:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:36:31: #1 tags after filtering in treatment: 14284226 INFO @ Sat, 11 Dec 2021 09:36:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:36:31: #1 finished! INFO @ Sat, 11 Dec 2021 09:36:31: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:36:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:36:33: #2 number of paired peaks: 13918 INFO @ Sat, 11 Dec 2021 09:36:33: start model_add_line... INFO @ Sat, 11 Dec 2021 09:36:33: start X-correlation... INFO @ Sat, 11 Dec 2021 09:36:33: end of X-cor INFO @ Sat, 11 Dec 2021 09:36:33: #2 finished! INFO @ Sat, 11 Dec 2021 09:36:33: #2 predicted fragment length is 131 bps INFO @ Sat, 11 Dec 2021 09:36:33: #2 alternative fragment length(s) may be 131 bps INFO @ Sat, 11 Dec 2021 09:36:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.10_model.r INFO @ Sat, 11 Dec 2021 09:36:33: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:36:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:36:34: 10000000 INFO @ Sat, 11 Dec 2021 09:36:35: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:36:39: 11000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 09:36:45: 12000000 INFO @ Sat, 11 Dec 2021 09:36:50: 13000000 INFO @ Sat, 11 Dec 2021 09:36:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.05_peaks.xls INFO @ Sat, 11 Dec 2021 09:36:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:36:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.05_summits.bed INFO @ Sat, 11 Dec 2021 09:36:52: Done! pass1 - making usageList (178 chroms): 3 millis pass2 - checking and writing primary data (10602 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:36:55: 14000000 INFO @ Sat, 11 Dec 2021 09:36:57: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:36:57: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:36:57: #1 total tags in treatment: 14284373 INFO @ Sat, 11 Dec 2021 09:36:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:36:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:36:58: #1 tags after filtering in treatment: 14284226 INFO @ Sat, 11 Dec 2021 09:36:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:36:58: #1 finished! INFO @ Sat, 11 Dec 2021 09:36:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:36:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:36:59: #2 number of paired peaks: 13918 INFO @ Sat, 11 Dec 2021 09:36:59: start model_add_line... INFO @ Sat, 11 Dec 2021 09:37:00: start X-correlation... INFO @ Sat, 11 Dec 2021 09:37:00: end of X-cor INFO @ Sat, 11 Dec 2021 09:37:00: #2 finished! INFO @ Sat, 11 Dec 2021 09:37:00: #2 predicted fragment length is 131 bps INFO @ Sat, 11 Dec 2021 09:37:00: #2 alternative fragment length(s) may be 131 bps INFO @ Sat, 11 Dec 2021 09:37:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.20_model.r INFO @ Sat, 11 Dec 2021 09:37:00: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:37:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:37:08: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:37:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.10_peaks.xls INFO @ Sat, 11 Dec 2021 09:37:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:37:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.10_summits.bed INFO @ Sat, 11 Dec 2021 09:37:24: Done! pass1 - making usageList (113 chroms): 2 millis pass2 - checking and writing primary data (9240 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:37:36: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:37:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.20_peaks.xls INFO @ Sat, 11 Dec 2021 09:37:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:37:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9434469/SRX9434469.20_summits.bed INFO @ Sat, 11 Dec 2021 09:37:52: Done! pass1 - making usageList (66 chroms): 1 millis pass2 - checking and writing primary data (7688 records, 4 fields): 12 millis CompletedMACS2peakCalling