Job ID = 14171092 SRX = SRX9434468 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:46 33854498 reads; of these: 33854498 (100.00%) were unpaired; of these: 730331 (2.16%) aligned 0 times 26146879 (77.23%) aligned exactly 1 time 6977288 (20.61%) aligned >1 times 97.84% overall alignment rate Time searching: 00:06:46 Overall time: 00:06:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 12246104 / 33124167 = 0.3697 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:41:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:41:52: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:41:52: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:41:57: 1000000 INFO @ Sat, 11 Dec 2021 09:42:02: 2000000 INFO @ Sat, 11 Dec 2021 09:42:08: 3000000 INFO @ Sat, 11 Dec 2021 09:42:13: 4000000 INFO @ Sat, 11 Dec 2021 09:42:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:42:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:42:22: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:42:22: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:42:23: 6000000 INFO @ Sat, 11 Dec 2021 09:42:29: 7000000 INFO @ Sat, 11 Dec 2021 09:42:29: 1000000 INFO @ Sat, 11 Dec 2021 09:42:35: 8000000 INFO @ Sat, 11 Dec 2021 09:42:36: 2000000 INFO @ Sat, 11 Dec 2021 09:42:41: 9000000 INFO @ Sat, 11 Dec 2021 09:42:43: 3000000 INFO @ Sat, 11 Dec 2021 09:42:47: 10000000 INFO @ Sat, 11 Dec 2021 09:42:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:42:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:42:52: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:42:52: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:42:53: 11000000 INFO @ Sat, 11 Dec 2021 09:42:57: 5000000 INFO @ Sat, 11 Dec 2021 09:42:58: 1000000 INFO @ Sat, 11 Dec 2021 09:42:59: 12000000 INFO @ Sat, 11 Dec 2021 09:43:04: 6000000 INFO @ Sat, 11 Dec 2021 09:43:04: 2000000 INFO @ Sat, 11 Dec 2021 09:43:05: 13000000 INFO @ Sat, 11 Dec 2021 09:43:11: 3000000 INFO @ Sat, 11 Dec 2021 09:43:11: 14000000 INFO @ Sat, 11 Dec 2021 09:43:11: 7000000 INFO @ Sat, 11 Dec 2021 09:43:17: 4000000 INFO @ Sat, 11 Dec 2021 09:43:17: 15000000 INFO @ Sat, 11 Dec 2021 09:43:18: 8000000 INFO @ Sat, 11 Dec 2021 09:43:23: 16000000 INFO @ Sat, 11 Dec 2021 09:43:23: 5000000 INFO @ Sat, 11 Dec 2021 09:43:25: 9000000 INFO @ Sat, 11 Dec 2021 09:43:29: 6000000 INFO @ Sat, 11 Dec 2021 09:43:29: 17000000 INFO @ Sat, 11 Dec 2021 09:43:32: 10000000 INFO @ Sat, 11 Dec 2021 09:43:35: 7000000 INFO @ Sat, 11 Dec 2021 09:43:35: 18000000 INFO @ Sat, 11 Dec 2021 09:43:39: 11000000 INFO @ Sat, 11 Dec 2021 09:43:41: 8000000 INFO @ Sat, 11 Dec 2021 09:43:41: 19000000 INFO @ Sat, 11 Dec 2021 09:43:46: 12000000 INFO @ Sat, 11 Dec 2021 09:43:47: 9000000 INFO @ Sat, 11 Dec 2021 09:43:47: 20000000 INFO @ Sat, 11 Dec 2021 09:43:53: 13000000 INFO @ Sat, 11 Dec 2021 09:43:53: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:43:53: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:43:53: #1 total tags in treatment: 20878063 INFO @ Sat, 11 Dec 2021 09:43:53: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:43:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:43:53: 10000000 INFO @ Sat, 11 Dec 2021 09:43:53: #1 tags after filtering in treatment: 20877990 INFO @ Sat, 11 Dec 2021 09:43:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:43:53: #1 finished! INFO @ Sat, 11 Dec 2021 09:43:53: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:43:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:43:55: #2 number of paired peaks: 8091 INFO @ Sat, 11 Dec 2021 09:43:55: start model_add_line... INFO @ Sat, 11 Dec 2021 09:43:56: start X-correlation... INFO @ Sat, 11 Dec 2021 09:43:56: end of X-cor INFO @ Sat, 11 Dec 2021 09:43:56: #2 finished! INFO @ Sat, 11 Dec 2021 09:43:56: #2 predicted fragment length is 134 bps INFO @ Sat, 11 Dec 2021 09:43:56: #2 alternative fragment length(s) may be 134 bps INFO @ Sat, 11 Dec 2021 09:43:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.05_model.r INFO @ Sat, 11 Dec 2021 09:43:56: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:43:56: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:43:59: 11000000 INFO @ Sat, 11 Dec 2021 09:43:59: 14000000 INFO @ Sat, 11 Dec 2021 09:44:05: 12000000 INFO @ Sat, 11 Dec 2021 09:44:06: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:44:11: 13000000 INFO @ Sat, 11 Dec 2021 09:44:13: 16000000 INFO @ Sat, 11 Dec 2021 09:44:17: 14000000 INFO @ Sat, 11 Dec 2021 09:44:21: 17000000 INFO @ Sat, 11 Dec 2021 09:44:23: 15000000 INFO @ Sat, 11 Dec 2021 09:44:27: 18000000 INFO @ Sat, 11 Dec 2021 09:44:29: 16000000 INFO @ Sat, 11 Dec 2021 09:44:34: 19000000 INFO @ Sat, 11 Dec 2021 09:44:35: 17000000 INFO @ Sat, 11 Dec 2021 09:44:38: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 09:44:41: 18000000 INFO @ Sat, 11 Dec 2021 09:44:41: 20000000 INFO @ Sat, 11 Dec 2021 09:44:47: 19000000 INFO @ Sat, 11 Dec 2021 09:44:47: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:44:47: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:44:47: #1 total tags in treatment: 20878063 INFO @ Sat, 11 Dec 2021 09:44:47: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:44:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:44:48: #1 tags after filtering in treatment: 20877990 INFO @ Sat, 11 Dec 2021 09:44:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:44:48: #1 finished! INFO @ Sat, 11 Dec 2021 09:44:48: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:44:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:44:50: #2 number of paired peaks: 8091 INFO @ Sat, 11 Dec 2021 09:44:50: start model_add_line... INFO @ Sat, 11 Dec 2021 09:44:50: start X-correlation... INFO @ Sat, 11 Dec 2021 09:44:50: end of X-cor INFO @ Sat, 11 Dec 2021 09:44:50: #2 finished! INFO @ Sat, 11 Dec 2021 09:44:50: #2 predicted fragment length is 134 bps INFO @ Sat, 11 Dec 2021 09:44:50: #2 alternative fragment length(s) may be 134 bps INFO @ Sat, 11 Dec 2021 09:44:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.10_model.r INFO @ Sat, 11 Dec 2021 09:44:50: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:44:50: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:44:52: 20000000 INFO @ Sat, 11 Dec 2021 09:44:58: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:44:58: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:44:58: #1 total tags in treatment: 20878063 INFO @ Sat, 11 Dec 2021 09:44:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:44:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:44:58: #1 tags after filtering in treatment: 20877990 INFO @ Sat, 11 Dec 2021 09:44:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:44:58: #1 finished! INFO @ Sat, 11 Dec 2021 09:44:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:44:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:44:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.05_peaks.xls INFO @ Sat, 11 Dec 2021 09:44:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:44:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.05_summits.bed INFO @ Sat, 11 Dec 2021 09:44:59: Done! pass1 - making usageList (599 chroms): 3 millis pass2 - checking and writing primary data (12593 records, 4 fields): 26 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:45:00: #2 number of paired peaks: 8091 INFO @ Sat, 11 Dec 2021 09:45:00: start model_add_line... INFO @ Sat, 11 Dec 2021 09:45:00: start X-correlation... INFO @ Sat, 11 Dec 2021 09:45:00: end of X-cor INFO @ Sat, 11 Dec 2021 09:45:00: #2 finished! INFO @ Sat, 11 Dec 2021 09:45:00: #2 predicted fragment length is 134 bps INFO @ Sat, 11 Dec 2021 09:45:00: #2 alternative fragment length(s) may be 134 bps INFO @ Sat, 11 Dec 2021 09:45:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.20_model.r INFO @ Sat, 11 Dec 2021 09:45:00: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:45:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:45:36: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:45:45: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:45:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.10_peaks.xls INFO @ Sat, 11 Dec 2021 09:45:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:45:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.10_summits.bed INFO @ Sat, 11 Dec 2021 09:45:57: Done! pass1 - making usageList (485 chroms): 2 millis pass2 - checking and writing primary data (10348 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:46:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.20_peaks.xls INFO @ Sat, 11 Dec 2021 09:46:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:46:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9434468/SRX9434468.20_summits.bed INFO @ Sat, 11 Dec 2021 09:46:04: Done! pass1 - making usageList (218 chroms): 2 millis pass2 - checking and writing primary data (7660 records, 4 fields): 13 millis CompletedMACS2peakCalling