Job ID = 14172475 SRX = SRX9427718 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:33 20501271 reads; of these: 20501271 (100.00%) were unpaired; of these: 819126 (4.00%) aligned 0 times 4101899 (20.01%) aligned exactly 1 time 15580246 (76.00%) aligned >1 times 96.00% overall alignment rate Time searching: 00:07:33 Overall time: 00:07:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8390482 / 19682145 = 0.4263 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:45:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:45:16: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:45:16: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:45:21: 1000000 INFO @ Sat, 11 Dec 2021 15:45:26: 2000000 INFO @ Sat, 11 Dec 2021 15:45:30: 3000000 INFO @ Sat, 11 Dec 2021 15:45:35: 4000000 INFO @ Sat, 11 Dec 2021 15:45:40: 5000000 INFO @ Sat, 11 Dec 2021 15:45:44: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:45:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:45:46: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:45:46: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:45:49: 7000000 INFO @ Sat, 11 Dec 2021 15:45:51: 1000000 INFO @ Sat, 11 Dec 2021 15:45:55: 8000000 INFO @ Sat, 11 Dec 2021 15:45:56: 2000000 INFO @ Sat, 11 Dec 2021 15:46:00: 9000000 INFO @ Sat, 11 Dec 2021 15:46:01: 3000000 INFO @ Sat, 11 Dec 2021 15:46:05: 10000000 INFO @ Sat, 11 Dec 2021 15:46:06: 4000000 INFO @ Sat, 11 Dec 2021 15:46:10: 11000000 INFO @ Sat, 11 Dec 2021 15:46:11: 5000000 INFO @ Sat, 11 Dec 2021 15:46:12: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:46:12: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:46:12: #1 total tags in treatment: 11291663 INFO @ Sat, 11 Dec 2021 15:46:12: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:46:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:46:12: #1 tags after filtering in treatment: 11291654 INFO @ Sat, 11 Dec 2021 15:46:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:46:12: #1 finished! INFO @ Sat, 11 Dec 2021 15:46:12: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:46:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:46:14: #2 number of paired peaks: 8306 INFO @ Sat, 11 Dec 2021 15:46:14: start model_add_line... INFO @ Sat, 11 Dec 2021 15:46:14: start X-correlation... INFO @ Sat, 11 Dec 2021 15:46:14: end of X-cor INFO @ Sat, 11 Dec 2021 15:46:14: #2 finished! INFO @ Sat, 11 Dec 2021 15:46:14: #2 predicted fragment length is 133 bps INFO @ Sat, 11 Dec 2021 15:46:14: #2 alternative fragment length(s) may be 3,133 bps INFO @ Sat, 11 Dec 2021 15:46:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.05_model.r INFO @ Sat, 11 Dec 2021 15:46:14: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:46:14: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:46:16: 6000000 INFO @ Sat, 11 Dec 2021 15:46:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:46:16: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:46:16: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:46:21: 7000000 INFO @ Sat, 11 Dec 2021 15:46:21: 1000000 INFO @ Sat, 11 Dec 2021 15:46:26: 2000000 INFO @ Sat, 11 Dec 2021 15:46:27: 8000000 INFO @ Sat, 11 Dec 2021 15:46:31: 3000000 INFO @ Sat, 11 Dec 2021 15:46:32: 9000000 INFO @ Sat, 11 Dec 2021 15:46:36: 4000000 INFO @ Sat, 11 Dec 2021 15:46:37: 10000000 INFO @ Sat, 11 Dec 2021 15:46:41: 5000000 INFO @ Sat, 11 Dec 2021 15:46:42: 11000000 INFO @ Sat, 11 Dec 2021 15:46:42: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:46:43: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:46:43: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:46:43: #1 total tags in treatment: 11291663 INFO @ Sat, 11 Dec 2021 15:46:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:46:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:46:44: #1 tags after filtering in treatment: 11291654 INFO @ Sat, 11 Dec 2021 15:46:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:46:44: #1 finished! INFO @ Sat, 11 Dec 2021 15:46:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:46:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:46:45: #2 number of paired peaks: 8306 INFO @ Sat, 11 Dec 2021 15:46:45: start model_add_line... INFO @ Sat, 11 Dec 2021 15:46:45: start X-correlation... INFO @ Sat, 11 Dec 2021 15:46:45: end of X-cor INFO @ Sat, 11 Dec 2021 15:46:45: #2 finished! INFO @ Sat, 11 Dec 2021 15:46:45: #2 predicted fragment length is 133 bps INFO @ Sat, 11 Dec 2021 15:46:45: #2 alternative fragment length(s) may be 3,133 bps INFO @ Sat, 11 Dec 2021 15:46:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.10_model.r INFO @ Sat, 11 Dec 2021 15:46:45: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:46:45: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:46:46: 6000000 INFO @ Sat, 11 Dec 2021 15:46:51: 7000000 INFO @ Sat, 11 Dec 2021 15:46:53: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:46:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:46:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.05_summits.bed INFO @ Sat, 11 Dec 2021 15:46:53: Done! pass1 - making usageList (1363 chroms): 2 millis pass2 - checking and writing primary data (7568 records, 4 fields): 38 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:46:56: 8000000 INFO @ Sat, 11 Dec 2021 15:47:01: 9000000 INFO @ Sat, 11 Dec 2021 15:47:06: 10000000 INFO @ Sat, 11 Dec 2021 15:47:11: 11000000 INFO @ Sat, 11 Dec 2021 15:47:13: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:47:13: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:47:13: #1 total tags in treatment: 11291663 INFO @ Sat, 11 Dec 2021 15:47:13: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:47:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:47:13: #1 tags after filtering in treatment: 11291654 INFO @ Sat, 11 Dec 2021 15:47:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:47:13: #1 finished! INFO @ Sat, 11 Dec 2021 15:47:13: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:47:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:47:14: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:47:14: #2 number of paired peaks: 8306 INFO @ Sat, 11 Dec 2021 15:47:14: start model_add_line... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:47:15: start X-correlation... INFO @ Sat, 11 Dec 2021 15:47:15: end of X-cor INFO @ Sat, 11 Dec 2021 15:47:15: #2 finished! INFO @ Sat, 11 Dec 2021 15:47:15: #2 predicted fragment length is 133 bps INFO @ Sat, 11 Dec 2021 15:47:15: #2 alternative fragment length(s) may be 3,133 bps INFO @ Sat, 11 Dec 2021 15:47:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.20_model.r INFO @ Sat, 11 Dec 2021 15:47:15: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:47:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:47:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:47:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:47:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.10_summits.bed INFO @ Sat, 11 Dec 2021 15:47:25: Done! pass1 - making usageList (1240 chroms): 2 millis pass2 - checking and writing primary data (4852 records, 4 fields): 32 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:47:43: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:47:54: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:47:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:47:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9427718/SRX9427718.20_summits.bed INFO @ Sat, 11 Dec 2021 15:47:54: Done! pass1 - making usageList (1033 chroms): 1 millis pass2 - checking and writing primary data (2597 records, 4 fields): 27 millis CompletedMACS2peakCalling