Job ID = 14172449 SRX = SRX9427716 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:17 17450363 reads; of these: 17450363 (100.00%) were unpaired; of these: 648368 (3.72%) aligned 0 times 4648689 (26.64%) aligned exactly 1 time 12153306 (69.65%) aligned >1 times 96.28% overall alignment rate Time searching: 00:08:17 Overall time: 00:08:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6184686 / 16801995 = 0.3681 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:40:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:40:24: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:40:24: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:40:34: 1000000 INFO @ Sat, 11 Dec 2021 15:40:43: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:40:53: 3000000 INFO @ Sat, 11 Dec 2021 15:40:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:40:54: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:40:54: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:41:03: 1000000 INFO @ Sat, 11 Dec 2021 15:41:03: 4000000 INFO @ Sat, 11 Dec 2021 15:41:11: 2000000 INFO @ Sat, 11 Dec 2021 15:41:13: 5000000 INFO @ Sat, 11 Dec 2021 15:41:20: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:41:23: 6000000 INFO @ Sat, 11 Dec 2021 15:41:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:41:24: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:41:24: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:41:28: 4000000 INFO @ Sat, 11 Dec 2021 15:41:33: 1000000 INFO @ Sat, 11 Dec 2021 15:41:33: 7000000 INFO @ Sat, 11 Dec 2021 15:41:37: 5000000 INFO @ Sat, 11 Dec 2021 15:41:42: 2000000 INFO @ Sat, 11 Dec 2021 15:41:44: 8000000 INFO @ Sat, 11 Dec 2021 15:41:46: 6000000 INFO @ Sat, 11 Dec 2021 15:41:50: 3000000 INFO @ Sat, 11 Dec 2021 15:41:55: 9000000 INFO @ Sat, 11 Dec 2021 15:41:55: 7000000 INFO @ Sat, 11 Dec 2021 15:41:59: 4000000 INFO @ Sat, 11 Dec 2021 15:42:04: 8000000 INFO @ Sat, 11 Dec 2021 15:42:04: 10000000 INFO @ Sat, 11 Dec 2021 15:42:07: 5000000 INFO @ Sat, 11 Dec 2021 15:42:10: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:42:10: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:42:10: #1 total tags in treatment: 10617309 INFO @ Sat, 11 Dec 2021 15:42:10: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:42:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:42:11: #1 tags after filtering in treatment: 10617301 INFO @ Sat, 11 Dec 2021 15:42:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:42:11: #1 finished! INFO @ Sat, 11 Dec 2021 15:42:11: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:42:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:42:13: 9000000 INFO @ Sat, 11 Dec 2021 15:42:13: #2 number of paired peaks: 6721 INFO @ Sat, 11 Dec 2021 15:42:13: start model_add_line... INFO @ Sat, 11 Dec 2021 15:42:13: start X-correlation... INFO @ Sat, 11 Dec 2021 15:42:13: end of X-cor INFO @ Sat, 11 Dec 2021 15:42:13: #2 finished! INFO @ Sat, 11 Dec 2021 15:42:13: #2 predicted fragment length is 126 bps INFO @ Sat, 11 Dec 2021 15:42:13: #2 alternative fragment length(s) may be 3,126 bps INFO @ Sat, 11 Dec 2021 15:42:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.05_model.r INFO @ Sat, 11 Dec 2021 15:42:13: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:42:13: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:42:16: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:42:21: 10000000 INFO @ Sat, 11 Dec 2021 15:42:25: 7000000 INFO @ Sat, 11 Dec 2021 15:42:26: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:42:26: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:42:26: #1 total tags in treatment: 10617309 INFO @ Sat, 11 Dec 2021 15:42:26: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:42:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:42:26: #1 tags after filtering in treatment: 10617301 INFO @ Sat, 11 Dec 2021 15:42:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:42:26: #1 finished! INFO @ Sat, 11 Dec 2021 15:42:26: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:42:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:42:28: #2 number of paired peaks: 6721 INFO @ Sat, 11 Dec 2021 15:42:28: start model_add_line... INFO @ Sat, 11 Dec 2021 15:42:28: start X-correlation... INFO @ Sat, 11 Dec 2021 15:42:28: end of X-cor INFO @ Sat, 11 Dec 2021 15:42:28: #2 finished! INFO @ Sat, 11 Dec 2021 15:42:28: #2 predicted fragment length is 126 bps INFO @ Sat, 11 Dec 2021 15:42:28: #2 alternative fragment length(s) may be 3,126 bps INFO @ Sat, 11 Dec 2021 15:42:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.10_model.r INFO @ Sat, 11 Dec 2021 15:42:28: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:42:28: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:42:34: 8000000 INFO @ Sat, 11 Dec 2021 15:42:42: 9000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:42:49: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:42:50: 10000000 INFO @ Sat, 11 Dec 2021 15:42:55: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:42:55: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:42:55: #1 total tags in treatment: 10617309 INFO @ Sat, 11 Dec 2021 15:42:55: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:42:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:42:56: #1 tags after filtering in treatment: 10617301 INFO @ Sat, 11 Dec 2021 15:42:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:42:56: #1 finished! INFO @ Sat, 11 Dec 2021 15:42:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:42:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:42:58: #2 number of paired peaks: 6721 INFO @ Sat, 11 Dec 2021 15:42:58: start model_add_line... INFO @ Sat, 11 Dec 2021 15:42:58: start X-correlation... INFO @ Sat, 11 Dec 2021 15:42:58: end of X-cor INFO @ Sat, 11 Dec 2021 15:42:58: #2 finished! INFO @ Sat, 11 Dec 2021 15:42:58: #2 predicted fragment length is 126 bps INFO @ Sat, 11 Dec 2021 15:42:58: #2 alternative fragment length(s) may be 3,126 bps INFO @ Sat, 11 Dec 2021 15:42:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.20_model.r INFO @ Sat, 11 Dec 2021 15:42:58: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:42:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:43:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:43:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:43:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.05_summits.bed INFO @ Sat, 11 Dec 2021 15:43:02: Done! pass1 - making usageList (1256 chroms): 3 millis pass2 - checking and writing primary data (7070 records, 4 fields): 62 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:43:06: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:43:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:43:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:43:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.10_summits.bed INFO @ Sat, 11 Dec 2021 15:43:20: Done! pass1 - making usageList (1123 chroms): 3 millis pass2 - checking and writing primary data (4394 records, 4 fields): 54 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:43:34: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:43:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:43:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:43:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9427716/SRX9427716.20_summits.bed INFO @ Sat, 11 Dec 2021 15:43:47: Done! pass1 - making usageList (810 chroms): 2 millis pass2 - checking and writing primary data (1954 records, 4 fields): 34 millis CompletedMACS2peakCalling