Job ID = 14172456 SRX = SRX9427715 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:26 14570203 reads; of these: 14570203 (100.00%) were unpaired; of these: 557388 (3.83%) aligned 0 times 2976771 (20.43%) aligned exactly 1 time 11036044 (75.74%) aligned >1 times 96.17% overall alignment rate Time searching: 00:05:26 Overall time: 00:05:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5530842 / 14012815 = 0.3947 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:37:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:37:25: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:37:25: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:37:30: 1000000 INFO @ Sat, 11 Dec 2021 15:37:35: 2000000 INFO @ Sat, 11 Dec 2021 15:37:41: 3000000 INFO @ Sat, 11 Dec 2021 15:37:46: 4000000 INFO @ Sat, 11 Dec 2021 15:37:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:37:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:37:56: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:37:56: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:37:57: 6000000 INFO @ Sat, 11 Dec 2021 15:38:04: 1000000 INFO @ Sat, 11 Dec 2021 15:38:04: 7000000 INFO @ Sat, 11 Dec 2021 15:38:11: 8000000 INFO @ Sat, 11 Dec 2021 15:38:11: 2000000 INFO @ Sat, 11 Dec 2021 15:38:14: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:38:14: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:38:14: #1 total tags in treatment: 8481973 INFO @ Sat, 11 Dec 2021 15:38:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:38:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:38:15: #1 tags after filtering in treatment: 8481961 INFO @ Sat, 11 Dec 2021 15:38:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:38:15: #1 finished! INFO @ Sat, 11 Dec 2021 15:38:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:38:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:38:16: #2 number of paired peaks: 7832 INFO @ Sat, 11 Dec 2021 15:38:16: start model_add_line... INFO @ Sat, 11 Dec 2021 15:38:16: start X-correlation... INFO @ Sat, 11 Dec 2021 15:38:16: end of X-cor INFO @ Sat, 11 Dec 2021 15:38:16: #2 finished! INFO @ Sat, 11 Dec 2021 15:38:16: #2 predicted fragment length is 131 bps INFO @ Sat, 11 Dec 2021 15:38:16: #2 alternative fragment length(s) may be 3,131 bps INFO @ Sat, 11 Dec 2021 15:38:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.05_model.r INFO @ Sat, 11 Dec 2021 15:38:16: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:38:16: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:38:18: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:38:25: 4000000 INFO @ Sat, 11 Dec 2021 15:38:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:38:26: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:38:26: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:38:33: 5000000 INFO @ Sat, 11 Dec 2021 15:38:33: 1000000 INFO @ Sat, 11 Dec 2021 15:38:38: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:38:40: 2000000 INFO @ Sat, 11 Dec 2021 15:38:41: 6000000 INFO @ Sat, 11 Dec 2021 15:38:46: 3000000 INFO @ Sat, 11 Dec 2021 15:38:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:38:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:38:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.05_summits.bed INFO @ Sat, 11 Dec 2021 15:38:47: Done! pass1 - making usageList (1281 chroms): 2 millis pass2 - checking and writing primary data (7095 records, 4 fields): 38 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:38:48: 7000000 INFO @ Sat, 11 Dec 2021 15:38:53: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:38:56: 8000000 INFO @ Sat, 11 Dec 2021 15:38:59: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:38:59: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:38:59: #1 total tags in treatment: 8481973 INFO @ Sat, 11 Dec 2021 15:38:59: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:38:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:39:00: 5000000 INFO @ Sat, 11 Dec 2021 15:39:00: #1 tags after filtering in treatment: 8481961 INFO @ Sat, 11 Dec 2021 15:39:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:39:00: #1 finished! INFO @ Sat, 11 Dec 2021 15:39:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:39:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:39:01: #2 number of paired peaks: 7832 INFO @ Sat, 11 Dec 2021 15:39:01: start model_add_line... INFO @ Sat, 11 Dec 2021 15:39:01: start X-correlation... INFO @ Sat, 11 Dec 2021 15:39:01: end of X-cor INFO @ Sat, 11 Dec 2021 15:39:01: #2 finished! INFO @ Sat, 11 Dec 2021 15:39:01: #2 predicted fragment length is 131 bps INFO @ Sat, 11 Dec 2021 15:39:01: #2 alternative fragment length(s) may be 3,131 bps INFO @ Sat, 11 Dec 2021 15:39:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.10_model.r INFO @ Sat, 11 Dec 2021 15:39:01: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:39:01: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:39:06: 6000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:39:11: 7000000 INFO @ Sat, 11 Dec 2021 15:39:17: 8000000 INFO @ Sat, 11 Dec 2021 15:39:19: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:39:19: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:39:19: #1 total tags in treatment: 8481973 INFO @ Sat, 11 Dec 2021 15:39:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:39:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:39:20: #1 tags after filtering in treatment: 8481961 INFO @ Sat, 11 Dec 2021 15:39:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:39:20: #1 finished! INFO @ Sat, 11 Dec 2021 15:39:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:39:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:39:21: #2 number of paired peaks: 7832 INFO @ Sat, 11 Dec 2021 15:39:21: start model_add_line... INFO @ Sat, 11 Dec 2021 15:39:21: start X-correlation... INFO @ Sat, 11 Dec 2021 15:39:21: end of X-cor INFO @ Sat, 11 Dec 2021 15:39:21: #2 finished! INFO @ Sat, 11 Dec 2021 15:39:21: #2 predicted fragment length is 131 bps INFO @ Sat, 11 Dec 2021 15:39:21: #2 alternative fragment length(s) may be 3,131 bps INFO @ Sat, 11 Dec 2021 15:39:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.20_model.r INFO @ Sat, 11 Dec 2021 15:39:21: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:39:21: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:39:23: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:39:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:39:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:39:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.10_summits.bed INFO @ Sat, 11 Dec 2021 15:39:32: Done! pass1 - making usageList (1126 chroms): 2 millis pass2 - checking and writing primary data (4299 records, 4 fields): 32 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:39:43: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:39:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:39:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:39:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9427715/SRX9427715.20_summits.bed INFO @ Sat, 11 Dec 2021 15:39:51: Done! pass1 - making usageList (815 chroms): 2 millis pass2 - checking and writing primary data (1910 records, 4 fields): 21 millis CompletedMACS2peakCalling