Job ID = 14167740 SRX = SRX9090282 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:44 17019986 reads; of these: 17019986 (100.00%) were unpaired; of these: 783523 (4.60%) aligned 0 times 11893303 (69.88%) aligned exactly 1 time 4343160 (25.52%) aligned >1 times 95.40% overall alignment rate Time searching: 00:04:44 Overall time: 00:04:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7386413 / 16236463 = 0.4549 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:22:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:22:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:22:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:22:31: 1000000 INFO @ Fri, 10 Dec 2021 13:22:37: 2000000 INFO @ Fri, 10 Dec 2021 13:22:42: 3000000 INFO @ Fri, 10 Dec 2021 13:22:47: 4000000 INFO @ Fri, 10 Dec 2021 13:22:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:22:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:22:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:22:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:22:58: 6000000 INFO @ Fri, 10 Dec 2021 13:23:02: 1000000 INFO @ Fri, 10 Dec 2021 13:23:03: 7000000 INFO @ Fri, 10 Dec 2021 13:23:08: 2000000 INFO @ Fri, 10 Dec 2021 13:23:09: 8000000 INFO @ Fri, 10 Dec 2021 13:23:14: 3000000 INFO @ Fri, 10 Dec 2021 13:23:14: #1 tag size is determined as 49 bps INFO @ Fri, 10 Dec 2021 13:23:14: #1 tag size = 49 INFO @ Fri, 10 Dec 2021 13:23:14: #1 total tags in treatment: 8850050 INFO @ Fri, 10 Dec 2021 13:23:14: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:23:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:23:15: #1 tags after filtering in treatment: 8849929 INFO @ Fri, 10 Dec 2021 13:23:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:23:15: #1 finished! INFO @ Fri, 10 Dec 2021 13:23:15: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:23:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:23:16: #2 number of paired peaks: 8091 INFO @ Fri, 10 Dec 2021 13:23:16: start model_add_line... INFO @ Fri, 10 Dec 2021 13:23:16: start X-correlation... INFO @ Fri, 10 Dec 2021 13:23:16: end of X-cor INFO @ Fri, 10 Dec 2021 13:23:16: #2 finished! INFO @ Fri, 10 Dec 2021 13:23:16: #2 predicted fragment length is 136 bps INFO @ Fri, 10 Dec 2021 13:23:16: #2 alternative fragment length(s) may be 136 bps INFO @ Fri, 10 Dec 2021 13:23:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.05_model.r INFO @ Fri, 10 Dec 2021 13:23:16: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:23:16: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:23:19: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:23:25: 5000000 INFO @ Fri, 10 Dec 2021 13:23:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:23:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:23:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:23:31: 6000000 INFO @ Fri, 10 Dec 2021 13:23:32: 1000000 INFO @ Fri, 10 Dec 2021 13:23:37: 7000000 INFO @ Fri, 10 Dec 2021 13:23:38: 2000000 INFO @ Fri, 10 Dec 2021 13:23:41: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:23:44: 3000000 INFO @ Fri, 10 Dec 2021 13:23:44: 8000000 INFO @ Fri, 10 Dec 2021 13:23:49: 4000000 INFO @ Fri, 10 Dec 2021 13:23:50: #1 tag size is determined as 49 bps INFO @ Fri, 10 Dec 2021 13:23:50: #1 tag size = 49 INFO @ Fri, 10 Dec 2021 13:23:50: #1 total tags in treatment: 8850050 INFO @ Fri, 10 Dec 2021 13:23:50: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:23:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:23:50: #1 tags after filtering in treatment: 8849929 INFO @ Fri, 10 Dec 2021 13:23:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:23:50: #1 finished! INFO @ Fri, 10 Dec 2021 13:23:50: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:23:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:23:51: #2 number of paired peaks: 8091 INFO @ Fri, 10 Dec 2021 13:23:51: start model_add_line... INFO @ Fri, 10 Dec 2021 13:23:51: start X-correlation... INFO @ Fri, 10 Dec 2021 13:23:51: end of X-cor INFO @ Fri, 10 Dec 2021 13:23:51: #2 finished! INFO @ Fri, 10 Dec 2021 13:23:51: #2 predicted fragment length is 136 bps INFO @ Fri, 10 Dec 2021 13:23:51: #2 alternative fragment length(s) may be 136 bps INFO @ Fri, 10 Dec 2021 13:23:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.10_model.r INFO @ Fri, 10 Dec 2021 13:23:51: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:23:51: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:23:53: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.05_peaks.xls INFO @ Fri, 10 Dec 2021 13:23:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:23:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.05_summits.bed INFO @ Fri, 10 Dec 2021 13:23:53: Done! pass1 - making usageList (421 chroms): 2 millis pass2 - checking and writing primary data (8338 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:23:55: 5000000 INFO @ Fri, 10 Dec 2021 13:24:00: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:24:06: 7000000 INFO @ Fri, 10 Dec 2021 13:24:12: 8000000 INFO @ Fri, 10 Dec 2021 13:24:16: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:24:17: #1 tag size is determined as 49 bps INFO @ Fri, 10 Dec 2021 13:24:17: #1 tag size = 49 INFO @ Fri, 10 Dec 2021 13:24:17: #1 total tags in treatment: 8850050 INFO @ Fri, 10 Dec 2021 13:24:17: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:24:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:24:17: #1 tags after filtering in treatment: 8849929 INFO @ Fri, 10 Dec 2021 13:24:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:24:17: #1 finished! INFO @ Fri, 10 Dec 2021 13:24:17: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:24:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:24:19: #2 number of paired peaks: 8091 INFO @ Fri, 10 Dec 2021 13:24:19: start model_add_line... INFO @ Fri, 10 Dec 2021 13:24:19: start X-correlation... INFO @ Fri, 10 Dec 2021 13:24:19: end of X-cor INFO @ Fri, 10 Dec 2021 13:24:19: #2 finished! INFO @ Fri, 10 Dec 2021 13:24:19: #2 predicted fragment length is 136 bps INFO @ Fri, 10 Dec 2021 13:24:19: #2 alternative fragment length(s) may be 136 bps INFO @ Fri, 10 Dec 2021 13:24:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.20_model.r INFO @ Fri, 10 Dec 2021 13:24:19: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:24:19: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:24:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.10_peaks.xls INFO @ Fri, 10 Dec 2021 13:24:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:24:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.10_summits.bed INFO @ Fri, 10 Dec 2021 13:24:30: Done! pass1 - making usageList (277 chroms): 3 millis pass2 - checking and writing primary data (6795 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:24:44: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:24:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.20_peaks.xls INFO @ Fri, 10 Dec 2021 13:24:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:24:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX9090282/SRX9090282.20_summits.bed INFO @ Fri, 10 Dec 2021 13:24:56: Done! pass1 - making usageList (212 chroms): 2 millis pass2 - checking and writing primary data (5067 records, 4 fields): 14 millis CompletedMACS2peakCalling