Job ID = 14170823 SRX = SRX8784758 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:41 6131067 reads; of these: 6131067 (100.00%) were unpaired; of these: 276754 (4.51%) aligned 0 times 5612195 (91.54%) aligned exactly 1 time 242118 (3.95%) aligned >1 times 95.49% overall alignment rate Time searching: 00:02:41 Overall time: 00:02:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 386273 / 5854313 = 0.0660 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:56:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:56:15: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:56:15: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:56:28: 1000000 INFO @ Sat, 11 Dec 2021 08:56:39: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:56:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:56:46: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:56:46: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:56:51: 3000000 INFO @ Sat, 11 Dec 2021 08:56:58: 1000000 INFO @ Sat, 11 Dec 2021 08:57:02: 4000000 INFO @ Sat, 11 Dec 2021 08:57:11: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:57:13: 5000000 INFO @ Sat, 11 Dec 2021 08:57:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:57:15: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:57:15: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:57:19: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 08:57:19: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 08:57:19: #1 total tags in treatment: 5468040 INFO @ Sat, 11 Dec 2021 08:57:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:57:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:57:19: #1 tags after filtering in treatment: 5467335 INFO @ Sat, 11 Dec 2021 08:57:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:57:19: #1 finished! INFO @ Sat, 11 Dec 2021 08:57:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:57:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:57:19: #2 number of paired peaks: 1490 INFO @ Sat, 11 Dec 2021 08:57:19: start model_add_line... INFO @ Sat, 11 Dec 2021 08:57:20: start X-correlation... INFO @ Sat, 11 Dec 2021 08:57:20: end of X-cor INFO @ Sat, 11 Dec 2021 08:57:20: #2 finished! INFO @ Sat, 11 Dec 2021 08:57:20: #2 predicted fragment length is 250 bps INFO @ Sat, 11 Dec 2021 08:57:20: #2 alternative fragment length(s) may be 250 bps INFO @ Sat, 11 Dec 2021 08:57:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.05_model.r INFO @ Sat, 11 Dec 2021 08:57:20: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:57:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:57:22: 3000000 INFO @ Sat, 11 Dec 2021 08:57:26: 1000000 INFO @ Sat, 11 Dec 2021 08:57:33: 4000000 INFO @ Sat, 11 Dec 2021 08:57:34: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:57:37: 2000000 INFO @ Sat, 11 Dec 2021 08:57:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.05_peaks.xls INFO @ Sat, 11 Dec 2021 08:57:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:57:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.05_summits.bed INFO @ Sat, 11 Dec 2021 08:57:43: Done! pass1 - making usageList (37 chroms): 2 millis pass2 - checking and writing primary data (6498 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:57:44: 5000000 INFO @ Sat, 11 Dec 2021 08:57:49: 3000000 INFO @ Sat, 11 Dec 2021 08:57:49: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 08:57:49: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 08:57:49: #1 total tags in treatment: 5468040 INFO @ Sat, 11 Dec 2021 08:57:49: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:57:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:57:50: #1 tags after filtering in treatment: 5467335 INFO @ Sat, 11 Dec 2021 08:57:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:57:50: #1 finished! INFO @ Sat, 11 Dec 2021 08:57:50: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:57:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:57:50: #2 number of paired peaks: 1490 INFO @ Sat, 11 Dec 2021 08:57:50: start model_add_line... INFO @ Sat, 11 Dec 2021 08:57:50: start X-correlation... INFO @ Sat, 11 Dec 2021 08:57:50: end of X-cor INFO @ Sat, 11 Dec 2021 08:57:50: #2 finished! INFO @ Sat, 11 Dec 2021 08:57:50: #2 predicted fragment length is 250 bps INFO @ Sat, 11 Dec 2021 08:57:50: #2 alternative fragment length(s) may be 250 bps INFO @ Sat, 11 Dec 2021 08:57:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.10_model.r INFO @ Sat, 11 Dec 2021 08:57:50: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:57:50: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 08:57:59: 4000000 INFO @ Sat, 11 Dec 2021 08:58:04: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:58:10: 5000000 INFO @ Sat, 11 Dec 2021 08:58:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.10_peaks.xls INFO @ Sat, 11 Dec 2021 08:58:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:58:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.10_summits.bed INFO @ Sat, 11 Dec 2021 08:58:13: Done! pass1 - making usageList (21 chroms): 1 millis pass2 - checking and writing primary data (2804 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:58:15: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 08:58:15: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 08:58:15: #1 total tags in treatment: 5468040 INFO @ Sat, 11 Dec 2021 08:58:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:58:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:58:16: #1 tags after filtering in treatment: 5467335 INFO @ Sat, 11 Dec 2021 08:58:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:58:16: #1 finished! INFO @ Sat, 11 Dec 2021 08:58:16: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:58:16: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 08:58:16: #2 number of paired peaks: 1490 INFO @ Sat, 11 Dec 2021 08:58:16: start model_add_line... INFO @ Sat, 11 Dec 2021 08:58:16: start X-correlation... INFO @ Sat, 11 Dec 2021 08:58:16: end of X-cor INFO @ Sat, 11 Dec 2021 08:58:16: #2 finished! INFO @ Sat, 11 Dec 2021 08:58:16: #2 predicted fragment length is 250 bps INFO @ Sat, 11 Dec 2021 08:58:16: #2 alternative fragment length(s) may be 250 bps INFO @ Sat, 11 Dec 2021 08:58:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.20_model.r INFO @ Sat, 11 Dec 2021 08:58:16: #3 Call peaks... INFO @ Sat, 11 Dec 2021 08:58:16: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 08:58:30: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 08:58:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.20_peaks.xls INFO @ Sat, 11 Dec 2021 08:58:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 08:58:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8784758/SRX8784758.20_summits.bed INFO @ Sat, 11 Dec 2021 08:58:38: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (864 records, 4 fields): 3 millis CompletedMACS2peakCalling