Job ID = 14168888 SRX = SRX8723975 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:08 50702107 reads; of these: 50702107 (100.00%) were unpaired; of these: 2301063 (4.54%) aligned 0 times 42779294 (84.37%) aligned exactly 1 time 5621750 (11.09%) aligned >1 times 95.46% overall alignment rate Time searching: 00:11:08 Overall time: 00:11:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12239751 / 48401044 = 0.2529 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:54:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:54:52: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:54:52: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:54:58: 1000000 INFO @ Fri, 10 Dec 2021 21:55:03: 2000000 INFO @ Fri, 10 Dec 2021 21:55:09: 3000000 INFO @ Fri, 10 Dec 2021 21:55:15: 4000000 INFO @ Fri, 10 Dec 2021 21:55:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:55:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:55:22: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:55:22: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:55:27: 6000000 INFO @ Fri, 10 Dec 2021 21:55:30: 1000000 INFO @ Fri, 10 Dec 2021 21:55:34: 7000000 INFO @ Fri, 10 Dec 2021 21:55:38: 2000000 INFO @ Fri, 10 Dec 2021 21:55:41: 8000000 INFO @ Fri, 10 Dec 2021 21:55:46: 3000000 INFO @ Fri, 10 Dec 2021 21:55:48: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:55:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:55:52: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:55:52: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:55:54: 4000000 INFO @ Fri, 10 Dec 2021 21:55:56: 10000000 INFO @ Fri, 10 Dec 2021 21:56:00: 1000000 INFO @ Fri, 10 Dec 2021 21:56:03: 5000000 INFO @ Fri, 10 Dec 2021 21:56:03: 11000000 INFO @ Fri, 10 Dec 2021 21:56:07: 2000000 INFO @ Fri, 10 Dec 2021 21:56:11: 12000000 INFO @ Fri, 10 Dec 2021 21:56:11: 6000000 INFO @ Fri, 10 Dec 2021 21:56:15: 3000000 INFO @ Fri, 10 Dec 2021 21:56:18: 13000000 INFO @ Fri, 10 Dec 2021 21:56:19: 7000000 INFO @ Fri, 10 Dec 2021 21:56:22: 4000000 INFO @ Fri, 10 Dec 2021 21:56:26: 14000000 INFO @ Fri, 10 Dec 2021 21:56:27: 8000000 INFO @ Fri, 10 Dec 2021 21:56:30: 5000000 INFO @ Fri, 10 Dec 2021 21:56:33: 15000000 INFO @ Fri, 10 Dec 2021 21:56:35: 9000000 INFO @ Fri, 10 Dec 2021 21:56:37: 6000000 INFO @ Fri, 10 Dec 2021 21:56:41: 16000000 INFO @ Fri, 10 Dec 2021 21:56:44: 10000000 INFO @ Fri, 10 Dec 2021 21:56:45: 7000000 INFO @ Fri, 10 Dec 2021 21:56:48: 17000000 INFO @ Fri, 10 Dec 2021 21:56:52: 11000000 INFO @ Fri, 10 Dec 2021 21:56:52: 8000000 INFO @ Fri, 10 Dec 2021 21:56:56: 18000000 INFO @ Fri, 10 Dec 2021 21:57:00: 9000000 INFO @ Fri, 10 Dec 2021 21:57:00: 12000000 INFO @ Fri, 10 Dec 2021 21:57:03: 19000000 INFO @ Fri, 10 Dec 2021 21:57:07: 10000000 INFO @ Fri, 10 Dec 2021 21:57:09: 13000000 INFO @ Fri, 10 Dec 2021 21:57:11: 20000000 INFO @ Fri, 10 Dec 2021 21:57:15: 11000000 INFO @ Fri, 10 Dec 2021 21:57:17: 14000000 INFO @ Fri, 10 Dec 2021 21:57:18: 21000000 INFO @ Fri, 10 Dec 2021 21:57:22: 12000000 INFO @ Fri, 10 Dec 2021 21:57:25: 15000000 INFO @ Fri, 10 Dec 2021 21:57:26: 22000000 INFO @ Fri, 10 Dec 2021 21:57:30: 13000000 INFO @ Fri, 10 Dec 2021 21:57:33: 23000000 INFO @ Fri, 10 Dec 2021 21:57:34: 16000000 INFO @ Fri, 10 Dec 2021 21:57:37: 14000000 INFO @ Fri, 10 Dec 2021 21:57:41: 24000000 INFO @ Fri, 10 Dec 2021 21:57:42: 17000000 INFO @ Fri, 10 Dec 2021 21:57:45: 15000000 INFO @ Fri, 10 Dec 2021 21:57:48: 25000000 INFO @ Fri, 10 Dec 2021 21:57:50: 18000000 INFO @ Fri, 10 Dec 2021 21:57:52: 16000000 INFO @ Fri, 10 Dec 2021 21:57:56: 26000000 INFO @ Fri, 10 Dec 2021 21:57:59: 19000000 INFO @ Fri, 10 Dec 2021 21:58:00: 17000000 INFO @ Fri, 10 Dec 2021 21:58:03: 27000000 INFO @ Fri, 10 Dec 2021 21:58:07: 20000000 INFO @ Fri, 10 Dec 2021 21:58:07: 18000000 INFO @ Fri, 10 Dec 2021 21:58:11: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 21:58:15: 19000000 INFO @ Fri, 10 Dec 2021 21:58:15: 21000000 INFO @ Fri, 10 Dec 2021 21:58:19: 29000000 INFO @ Fri, 10 Dec 2021 21:58:22: 20000000 INFO @ Fri, 10 Dec 2021 21:58:24: 22000000 INFO @ Fri, 10 Dec 2021 21:58:26: 30000000 INFO @ Fri, 10 Dec 2021 21:58:30: 21000000 INFO @ Fri, 10 Dec 2021 21:58:32: 23000000 INFO @ Fri, 10 Dec 2021 21:58:34: 31000000 INFO @ Fri, 10 Dec 2021 21:58:37: 22000000 INFO @ Fri, 10 Dec 2021 21:58:40: 24000000 INFO @ Fri, 10 Dec 2021 21:58:41: 32000000 INFO @ Fri, 10 Dec 2021 21:58:45: 23000000 INFO @ Fri, 10 Dec 2021 21:58:49: 25000000 INFO @ Fri, 10 Dec 2021 21:58:49: 33000000 INFO @ Fri, 10 Dec 2021 21:58:53: 24000000 INFO @ Fri, 10 Dec 2021 21:58:56: 34000000 INFO @ Fri, 10 Dec 2021 21:58:57: 26000000 INFO @ Fri, 10 Dec 2021 21:59:00: 25000000 INFO @ Fri, 10 Dec 2021 21:59:04: 35000000 INFO @ Fri, 10 Dec 2021 21:59:05: 27000000 INFO @ Fri, 10 Dec 2021 21:59:08: 26000000 INFO @ Fri, 10 Dec 2021 21:59:12: 36000000 INFO @ Fri, 10 Dec 2021 21:59:13: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 21:59:13: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 21:59:13: #1 total tags in treatment: 36161293 INFO @ Fri, 10 Dec 2021 21:59:13: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:59:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:59:14: 28000000 INFO @ Fri, 10 Dec 2021 21:59:14: #1 tags after filtering in treatment: 36161214 INFO @ Fri, 10 Dec 2021 21:59:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:59:14: #1 finished! INFO @ Fri, 10 Dec 2021 21:59:14: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:59:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:59:15: 27000000 INFO @ Fri, 10 Dec 2021 21:59:16: #2 number of paired peaks: 52 WARNING @ Fri, 10 Dec 2021 21:59:16: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 21:59:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:59:22: 29000000 INFO @ Fri, 10 Dec 2021 21:59:22: 28000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 21:59:30: 29000000 INFO @ Fri, 10 Dec 2021 21:59:30: 30000000 INFO @ Fri, 10 Dec 2021 21:59:37: 30000000 INFO @ Fri, 10 Dec 2021 21:59:39: 31000000 INFO @ Fri, 10 Dec 2021 21:59:45: 31000000 INFO @ Fri, 10 Dec 2021 21:59:47: 32000000 INFO @ Fri, 10 Dec 2021 21:59:52: 32000000 INFO @ Fri, 10 Dec 2021 21:59:55: 33000000 INFO @ Fri, 10 Dec 2021 21:59:59: 33000000 INFO @ Fri, 10 Dec 2021 22:00:04: 34000000 INFO @ Fri, 10 Dec 2021 22:00:07: 34000000 INFO @ Fri, 10 Dec 2021 22:00:12: 35000000 INFO @ Fri, 10 Dec 2021 22:00:14: 35000000 INFO @ Fri, 10 Dec 2021 22:00:20: 36000000 INFO @ Fri, 10 Dec 2021 22:00:22: 36000000 INFO @ Fri, 10 Dec 2021 22:00:22: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 22:00:22: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 22:00:22: #1 total tags in treatment: 36161293 INFO @ Fri, 10 Dec 2021 22:00:22: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:00:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:00:23: #1 tags after filtering in treatment: 36161214 INFO @ Fri, 10 Dec 2021 22:00:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 22:00:23: #1 finished! INFO @ Fri, 10 Dec 2021 22:00:23: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:00:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:00:23: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 22:00:23: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 22:00:23: #1 total tags in treatment: 36161293 INFO @ Fri, 10 Dec 2021 22:00:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:00:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:00:24: #1 tags after filtering in treatment: 36161214 INFO @ Fri, 10 Dec 2021 22:00:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 22:00:24: #1 finished! INFO @ Fri, 10 Dec 2021 22:00:24: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:00:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:00:25: #2 number of paired peaks: 52 WARNING @ Fri, 10 Dec 2021 22:00:25: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 22:00:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 22:00:26: #2 number of paired peaks: 52 WARNING @ Fri, 10 Dec 2021 22:00:26: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 22:00:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8723975/SRX8723975.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling