Job ID = 14168869 SRX = SRX8723974 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:03 25832934 reads; of these: 25832934 (100.00%) were unpaired; of these: 1582442 (6.13%) aligned 0 times 18133513 (70.20%) aligned exactly 1 time 6116979 (23.68%) aligned >1 times 93.87% overall alignment rate Time searching: 00:06:03 Overall time: 00:06:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13128753 / 24250492 = 0.5414 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:33:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:33:44: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:33:44: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:33:50: 1000000 INFO @ Fri, 10 Dec 2021 21:33:55: 2000000 INFO @ Fri, 10 Dec 2021 21:34:00: 3000000 INFO @ Fri, 10 Dec 2021 21:34:06: 4000000 INFO @ Fri, 10 Dec 2021 21:34:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:34:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:34:14: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:34:14: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:34:17: 6000000 INFO @ Fri, 10 Dec 2021 21:34:22: 1000000 INFO @ Fri, 10 Dec 2021 21:34:24: 7000000 INFO @ Fri, 10 Dec 2021 21:34:29: 2000000 INFO @ Fri, 10 Dec 2021 21:34:30: 8000000 INFO @ Fri, 10 Dec 2021 21:34:36: 3000000 INFO @ Fri, 10 Dec 2021 21:34:37: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:34:44: 10000000 INFO @ Fri, 10 Dec 2021 21:34:44: 4000000 INFO @ Fri, 10 Dec 2021 21:34:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:34:44: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:34:44: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:34:50: 11000000 INFO @ Fri, 10 Dec 2021 21:34:51: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 21:34:51: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 21:34:51: #1 total tags in treatment: 11121739 INFO @ Fri, 10 Dec 2021 21:34:51: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:34:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:34:52: 5000000 INFO @ Fri, 10 Dec 2021 21:34:52: 1000000 INFO @ Fri, 10 Dec 2021 21:34:52: #1 tags after filtering in treatment: 11121671 INFO @ Fri, 10 Dec 2021 21:34:52: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:34:52: #1 finished! INFO @ Fri, 10 Dec 2021 21:34:52: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:34:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:34:53: #2 number of paired peaks: 3897 INFO @ Fri, 10 Dec 2021 21:34:53: start model_add_line... INFO @ Fri, 10 Dec 2021 21:34:53: start X-correlation... INFO @ Fri, 10 Dec 2021 21:34:53: end of X-cor INFO @ Fri, 10 Dec 2021 21:34:53: #2 finished! INFO @ Fri, 10 Dec 2021 21:34:53: #2 predicted fragment length is 208 bps INFO @ Fri, 10 Dec 2021 21:34:53: #2 alternative fragment length(s) may be 208 bps INFO @ Fri, 10 Dec 2021 21:34:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.05_model.r INFO @ Fri, 10 Dec 2021 21:34:53: #3 Call peaks... INFO @ Fri, 10 Dec 2021 21:34:53: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 21:34:59: 6000000 INFO @ Fri, 10 Dec 2021 21:34:59: 2000000 INFO @ Fri, 10 Dec 2021 21:35:07: 3000000 INFO @ Fri, 10 Dec 2021 21:35:07: 7000000 INFO @ Fri, 10 Dec 2021 21:35:14: 4000000 INFO @ Fri, 10 Dec 2021 21:35:14: 8000000 INFO @ Fri, 10 Dec 2021 21:35:21: 5000000 INFO @ Fri, 10 Dec 2021 21:35:22: 9000000 INFO @ Fri, 10 Dec 2021 21:35:25: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 21:35:29: 6000000 INFO @ Fri, 10 Dec 2021 21:35:29: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 21:35:36: 7000000 INFO @ Fri, 10 Dec 2021 21:35:37: 11000000 INFO @ Fri, 10 Dec 2021 21:35:38: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 21:35:38: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 21:35:38: #1 total tags in treatment: 11121739 INFO @ Fri, 10 Dec 2021 21:35:38: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:35:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:35:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.05_peaks.xls INFO @ Fri, 10 Dec 2021 21:35:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 21:35:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.05_summits.bed INFO @ Fri, 10 Dec 2021 21:35:38: Done! INFO @ Fri, 10 Dec 2021 21:35:38: #1 tags after filtering in treatment: 11121671 INFO @ Fri, 10 Dec 2021 21:35:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:35:38: #1 finished! INFO @ Fri, 10 Dec 2021 21:35:38: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:35:38: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (677 chroms): 2 millis pass2 - checking and writing primary data (9169 records, 4 fields): 26 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:35:40: #2 number of paired peaks: 3897 INFO @ Fri, 10 Dec 2021 21:35:40: start model_add_line... INFO @ Fri, 10 Dec 2021 21:35:40: start X-correlation... INFO @ Fri, 10 Dec 2021 21:35:40: end of X-cor INFO @ Fri, 10 Dec 2021 21:35:40: #2 finished! INFO @ Fri, 10 Dec 2021 21:35:40: #2 predicted fragment length is 208 bps INFO @ Fri, 10 Dec 2021 21:35:40: #2 alternative fragment length(s) may be 208 bps INFO @ Fri, 10 Dec 2021 21:35:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.10_model.r INFO @ Fri, 10 Dec 2021 21:35:40: #3 Call peaks... INFO @ Fri, 10 Dec 2021 21:35:40: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 21:35:43: 8000000 INFO @ Fri, 10 Dec 2021 21:35:50: 9000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 21:35:56: 10000000 INFO @ Fri, 10 Dec 2021 21:36:03: 11000000 INFO @ Fri, 10 Dec 2021 21:36:04: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 21:36:04: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 21:36:04: #1 total tags in treatment: 11121739 INFO @ Fri, 10 Dec 2021 21:36:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:36:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:36:04: #1 tags after filtering in treatment: 11121671 INFO @ Fri, 10 Dec 2021 21:36:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:36:04: #1 finished! INFO @ Fri, 10 Dec 2021 21:36:04: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:36:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:36:05: #2 number of paired peaks: 3897 INFO @ Fri, 10 Dec 2021 21:36:05: start model_add_line... INFO @ Fri, 10 Dec 2021 21:36:06: start X-correlation... INFO @ Fri, 10 Dec 2021 21:36:06: end of X-cor INFO @ Fri, 10 Dec 2021 21:36:06: #2 finished! INFO @ Fri, 10 Dec 2021 21:36:06: #2 predicted fragment length is 208 bps INFO @ Fri, 10 Dec 2021 21:36:06: #2 alternative fragment length(s) may be 208 bps INFO @ Fri, 10 Dec 2021 21:36:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.20_model.r INFO @ Fri, 10 Dec 2021 21:36:06: #3 Call peaks... INFO @ Fri, 10 Dec 2021 21:36:06: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 21:36:12: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 21:36:25: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.10_peaks.xls INFO @ Fri, 10 Dec 2021 21:36:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 21:36:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.10_summits.bed INFO @ Fri, 10 Dec 2021 21:36:25: Done! pass1 - making usageList (475 chroms): 1 millis pass2 - checking and writing primary data (6387 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:36:37: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 21:36:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.20_peaks.xls INFO @ Fri, 10 Dec 2021 21:36:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 21:36:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8723974/SRX8723974.20_summits.bed INFO @ Fri, 10 Dec 2021 21:36:50: Done! pass1 - making usageList (331 chroms): 1 millis pass2 - checking and writing primary data (3416 records, 4 fields): 12 millis CompletedMACS2peakCalling