Job ID = 14168856 SRX = SRX8723971 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:14 22756793 reads; of these: 22756793 (100.00%) were unpaired; of these: 1999828 (8.79%) aligned 0 times 15417595 (67.75%) aligned exactly 1 time 5339370 (23.46%) aligned >1 times 91.21% overall alignment rate Time searching: 00:08:15 Overall time: 00:08:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4051099 / 20756965 = 0.1952 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:24:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:24:17: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:24:17: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:24:29: 1000000 INFO @ Fri, 10 Dec 2021 21:24:40: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:24:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:24:47: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:24:47: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:24:52: 3000000 INFO @ Fri, 10 Dec 2021 21:24:56: 1000000 INFO @ Fri, 10 Dec 2021 21:25:05: 4000000 INFO @ Fri, 10 Dec 2021 21:25:05: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:25:15: 3000000 INFO @ Fri, 10 Dec 2021 21:25:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:25:17: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:25:17: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:25:17: 5000000 INFO @ Fri, 10 Dec 2021 21:25:24: 4000000 INFO @ Fri, 10 Dec 2021 21:25:29: 1000000 INFO @ Fri, 10 Dec 2021 21:25:30: 6000000 INFO @ Fri, 10 Dec 2021 21:25:34: 5000000 INFO @ Fri, 10 Dec 2021 21:25:40: 2000000 INFO @ Fri, 10 Dec 2021 21:25:42: 7000000 INFO @ Fri, 10 Dec 2021 21:25:44: 6000000 INFO @ Fri, 10 Dec 2021 21:25:50: 3000000 INFO @ Fri, 10 Dec 2021 21:25:53: 7000000 INFO @ Fri, 10 Dec 2021 21:25:54: 8000000 INFO @ Fri, 10 Dec 2021 21:26:00: 4000000 INFO @ Fri, 10 Dec 2021 21:26:03: 8000000 INFO @ Fri, 10 Dec 2021 21:26:07: 9000000 INFO @ Fri, 10 Dec 2021 21:26:10: 5000000 INFO @ Fri, 10 Dec 2021 21:26:13: 9000000 INFO @ Fri, 10 Dec 2021 21:26:19: 10000000 INFO @ Fri, 10 Dec 2021 21:26:20: 6000000 INFO @ Fri, 10 Dec 2021 21:26:23: 10000000 INFO @ Fri, 10 Dec 2021 21:26:30: 7000000 INFO @ Fri, 10 Dec 2021 21:26:31: 11000000 INFO @ Fri, 10 Dec 2021 21:26:33: 11000000 INFO @ Fri, 10 Dec 2021 21:26:39: 8000000 INFO @ Fri, 10 Dec 2021 21:26:42: 12000000 INFO @ Fri, 10 Dec 2021 21:26:43: 12000000 INFO @ Fri, 10 Dec 2021 21:26:49: 9000000 INFO @ Fri, 10 Dec 2021 21:26:52: 13000000 INFO @ Fri, 10 Dec 2021 21:26:56: 13000000 INFO @ Fri, 10 Dec 2021 21:26:59: 10000000 INFO @ Fri, 10 Dec 2021 21:27:03: 14000000 INFO @ Fri, 10 Dec 2021 21:27:08: 14000000 INFO @ Fri, 10 Dec 2021 21:27:10: 11000000 INFO @ Fri, 10 Dec 2021 21:27:14: 15000000 INFO @ Fri, 10 Dec 2021 21:27:20: 12000000 INFO @ Fri, 10 Dec 2021 21:27:21: 15000000 INFO @ Fri, 10 Dec 2021 21:27:24: 16000000 INFO @ Fri, 10 Dec 2021 21:27:29: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 21:27:31: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 21:27:31: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 21:27:31: #1 total tags in treatment: 16705866 INFO @ Fri, 10 Dec 2021 21:27:31: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:27:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:27:31: #1 tags after filtering in treatment: 16705865 INFO @ Fri, 10 Dec 2021 21:27:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:27:31: #1 finished! INFO @ Fri, 10 Dec 2021 21:27:31: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:27:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:27:33: #2 number of paired peaks: 282 WARNING @ Fri, 10 Dec 2021 21:27:33: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Fri, 10 Dec 2021 21:27:33: start model_add_line... INFO @ Fri, 10 Dec 2021 21:27:33: 16000000 INFO @ Fri, 10 Dec 2021 21:27:33: start X-correlation... INFO @ Fri, 10 Dec 2021 21:27:33: end of X-cor INFO @ Fri, 10 Dec 2021 21:27:33: #2 finished! INFO @ Fri, 10 Dec 2021 21:27:33: #2 predicted fragment length is 101 bps INFO @ Fri, 10 Dec 2021 21:27:33: #2 alternative fragment length(s) may be 101 bps INFO @ Fri, 10 Dec 2021 21:27:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.10_model.r INFO @ Fri, 10 Dec 2021 21:27:33: #3 Call peaks... INFO @ Fri, 10 Dec 2021 21:27:33: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 21:27:39: 14000000 INFO @ Fri, 10 Dec 2021 21:27:42: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 21:27:42: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 21:27:42: #1 total tags in treatment: 16705866 INFO @ Fri, 10 Dec 2021 21:27:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:27:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:27:43: #1 tags after filtering in treatment: 16705865 INFO @ Fri, 10 Dec 2021 21:27:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:27:43: #1 finished! INFO @ Fri, 10 Dec 2021 21:27:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:27:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:27:44: #2 number of paired peaks: 282 WARNING @ Fri, 10 Dec 2021 21:27:44: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Fri, 10 Dec 2021 21:27:44: start model_add_line... INFO @ Fri, 10 Dec 2021 21:27:44: start X-correlation... INFO @ Fri, 10 Dec 2021 21:27:44: end of X-cor INFO @ Fri, 10 Dec 2021 21:27:44: #2 finished! INFO @ Fri, 10 Dec 2021 21:27:44: #2 predicted fragment length is 101 bps INFO @ Fri, 10 Dec 2021 21:27:44: #2 alternative fragment length(s) may be 101 bps INFO @ Fri, 10 Dec 2021 21:27:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.05_model.r INFO @ Fri, 10 Dec 2021 21:27:44: #3 Call peaks... INFO @ Fri, 10 Dec 2021 21:27:44: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 21:27:48: 15000000 INFO @ Fri, 10 Dec 2021 21:27:57: 16000000 INFO @ Fri, 10 Dec 2021 21:28:03: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 21:28:03: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 21:28:03: #1 total tags in treatment: 16705866 INFO @ Fri, 10 Dec 2021 21:28:03: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:28:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:28:04: #1 tags after filtering in treatment: 16705865 INFO @ Fri, 10 Dec 2021 21:28:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:28:04: #1 finished! INFO @ Fri, 10 Dec 2021 21:28:04: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:28:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:28:06: #2 number of paired peaks: 282 WARNING @ Fri, 10 Dec 2021 21:28:06: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Fri, 10 Dec 2021 21:28:06: start model_add_line... INFO @ Fri, 10 Dec 2021 21:28:06: start X-correlation... INFO @ Fri, 10 Dec 2021 21:28:06: end of X-cor INFO @ Fri, 10 Dec 2021 21:28:06: #2 finished! INFO @ Fri, 10 Dec 2021 21:28:06: #2 predicted fragment length is 101 bps INFO @ Fri, 10 Dec 2021 21:28:06: #2 alternative fragment length(s) may be 101 bps INFO @ Fri, 10 Dec 2021 21:28:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.20_model.r INFO @ Fri, 10 Dec 2021 21:28:06: #3 Call peaks... INFO @ Fri, 10 Dec 2021 21:28:06: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 21:28:23: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 21:28:31: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 21:28:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.10_peaks.xls INFO @ Fri, 10 Dec 2021 21:28:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 21:28:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.10_summits.bed INFO @ Fri, 10 Dec 2021 21:28:47: Done! pass1 - making usageList (348 chroms): 1 millis pass2 - checking and writing primary data (994 records, 4 fields): 38 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:28:55: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.05_peaks.xls INFO @ Fri, 10 Dec 2021 21:28:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 21:28:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.05_summits.bed INFO @ Fri, 10 Dec 2021 21:28:55: Done! pass1 - making usageList (466 chroms): 2 millis pass2 - checking and writing primary data (3854 records, 4 fields): 26 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:28:56: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 21:29:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.20_peaks.xls INFO @ Fri, 10 Dec 2021 21:29:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 21:29:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8723971/SRX8723971.20_summits.bed INFO @ Fri, 10 Dec 2021 21:29:20: Done! pass1 - making usageList (196 chroms): 2 millis pass2 - checking and writing primary data (392 records, 4 fields): 12 millis CompletedMACS2peakCalling