Job ID = 14170880 SRX = SRX8689046 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:26 6914006 reads; of these: 6914006 (100.00%) were unpaired; of these: 875635 (12.66%) aligned 0 times 5257183 (76.04%) aligned exactly 1 time 781188 (11.30%) aligned >1 times 87.34% overall alignment rate Time searching: 00:01:26 Overall time: 00:01:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 253261 / 6038371 = 0.0419 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:58:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:58:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:58:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:58:55: 1000000 INFO @ Sat, 11 Dec 2021 08:59:01: 2000000 INFO @ Sat, 11 Dec 2021 08:59:08: 3000000 INFO @ Sat, 11 Dec 2021 08:59:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:59:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:59:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:59:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:59:22: 5000000 INFO @ Sat, 11 Dec 2021 08:59:25: 1000000 INFO @ Sat, 11 Dec 2021 08:59:28: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 08:59:28: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 08:59:28: #1 total tags in treatment: 5785110 INFO @ Sat, 11 Dec 2021 08:59:28: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:59:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:59:28: #1 tags after filtering in treatment: 5784837 INFO @ Sat, 11 Dec 2021 08:59:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:59:28: #1 finished! INFO @ Sat, 11 Dec 2021 08:59:28: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:59:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:59:28: #2 number of paired peaks: 88 WARNING @ Sat, 11 Dec 2021 08:59:28: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:59:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:59:32: 2000000 INFO @ Sat, 11 Dec 2021 08:59:39: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:59:46: 4000000 INFO @ Sat, 11 Dec 2021 08:59:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:59:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:59:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:59:54: 5000000 INFO @ Sat, 11 Dec 2021 08:59:57: 1000000 INFO @ Sat, 11 Dec 2021 09:00:00: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:00:00: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:00:00: #1 total tags in treatment: 5785110 INFO @ Sat, 11 Dec 2021 09:00:00: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:00:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:00:01: #1 tags after filtering in treatment: 5784837 INFO @ Sat, 11 Dec 2021 09:00:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:00:01: #1 finished! INFO @ Sat, 11 Dec 2021 09:00:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:00:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:00:01: #2 number of paired peaks: 88 WARNING @ Sat, 11 Dec 2021 09:00:01: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:00:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:00:05: 2000000 INFO @ Sat, 11 Dec 2021 09:00:13: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:00:21: 4000000 INFO @ Sat, 11 Dec 2021 09:00:28: 5000000 INFO @ Sat, 11 Dec 2021 09:00:34: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:00:34: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:00:34: #1 total tags in treatment: 5785110 INFO @ Sat, 11 Dec 2021 09:00:34: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:00:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:00:35: #1 tags after filtering in treatment: 5784837 INFO @ Sat, 11 Dec 2021 09:00:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:00:35: #1 finished! INFO @ Sat, 11 Dec 2021 09:00:35: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:00:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:00:35: #2 number of paired peaks: 88 WARNING @ Sat, 11 Dec 2021 09:00:35: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:00:35: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8689046/SRX8689046.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling