Job ID = 14169099 SRX = SRX8688997 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:50 5758584 reads; of these: 5758584 (100.00%) were unpaired; of these: 479843 (8.33%) aligned 0 times 4630450 (80.41%) aligned exactly 1 time 648291 (11.26%) aligned >1 times 91.67% overall alignment rate Time searching: 00:01:51 Overall time: 00:01:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 207464 / 5278741 = 0.0393 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 23:45:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 23:45:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 23:45:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 23:45:34: 1000000 INFO @ Fri, 10 Dec 2021 23:45:42: 2000000 INFO @ Fri, 10 Dec 2021 23:45:51: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 23:45:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 23:45:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 23:45:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 23:45:59: 4000000 INFO @ Fri, 10 Dec 2021 23:46:05: 1000000 INFO @ Fri, 10 Dec 2021 23:46:09: 5000000 INFO @ Fri, 10 Dec 2021 23:46:09: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 23:46:09: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 23:46:09: #1 total tags in treatment: 5071277 INFO @ Fri, 10 Dec 2021 23:46:09: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 23:46:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 23:46:10: #1 tags after filtering in treatment: 5070993 INFO @ Fri, 10 Dec 2021 23:46:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 23:46:10: #1 finished! INFO @ Fri, 10 Dec 2021 23:46:10: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 23:46:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 23:46:10: #2 number of paired peaks: 79 WARNING @ Fri, 10 Dec 2021 23:46:10: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 23:46:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 23:46:13: 2000000 INFO @ Fri, 10 Dec 2021 23:46:21: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 23:46:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 23:46:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 23:46:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 23:46:29: 4000000 INFO @ Fri, 10 Dec 2021 23:46:35: 1000000 INFO @ Fri, 10 Dec 2021 23:46:38: 5000000 INFO @ Fri, 10 Dec 2021 23:46:39: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 23:46:39: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 23:46:39: #1 total tags in treatment: 5071277 INFO @ Fri, 10 Dec 2021 23:46:39: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 23:46:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 23:46:40: #1 tags after filtering in treatment: 5070993 INFO @ Fri, 10 Dec 2021 23:46:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 23:46:40: #1 finished! INFO @ Fri, 10 Dec 2021 23:46:40: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 23:46:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 23:46:40: #2 number of paired peaks: 79 WARNING @ Fri, 10 Dec 2021 23:46:40: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 23:46:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 23:46:43: 2000000 INFO @ Fri, 10 Dec 2021 23:46:51: 3000000 INFO @ Fri, 10 Dec 2021 23:47:00: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 23:47:09: 5000000 INFO @ Fri, 10 Dec 2021 23:47:10: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 23:47:10: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 23:47:10: #1 total tags in treatment: 5071277 INFO @ Fri, 10 Dec 2021 23:47:10: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 23:47:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 23:47:10: #1 tags after filtering in treatment: 5070993 INFO @ Fri, 10 Dec 2021 23:47:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 23:47:10: #1 finished! INFO @ Fri, 10 Dec 2021 23:47:10: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 23:47:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 23:47:11: #2 number of paired peaks: 79 WARNING @ Fri, 10 Dec 2021 23:47:11: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 23:47:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688997/SRX8688997.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。