Job ID = 14168979 SRX = SRX8688989 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:38 5948957 reads; of these: 5948957 (100.00%) were unpaired; of these: 187598 (3.15%) aligned 0 times 3999993 (67.24%) aligned exactly 1 time 1761366 (29.61%) aligned >1 times 96.85% overall alignment rate Time searching: 00:01:38 Overall time: 00:01:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 459448 / 5761359 = 0.0797 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 22:36:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 22:36:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 22:36:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 22:37:02: 1000000 INFO @ Fri, 10 Dec 2021 22:37:07: 2000000 INFO @ Fri, 10 Dec 2021 22:37:12: 3000000 INFO @ Fri, 10 Dec 2021 22:37:18: 4000000 INFO @ Fri, 10 Dec 2021 22:37:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 22:37:25: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 22:37:25: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 22:37:25: #1 total tags in treatment: 5301911 INFO @ Fri, 10 Dec 2021 22:37:25: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:37:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:37:26: #1 tags after filtering in treatment: 5301907 INFO @ Fri, 10 Dec 2021 22:37:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 22:37:26: #1 finished! INFO @ Fri, 10 Dec 2021 22:37:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:37:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:37:26: #2 number of paired peaks: 442 WARNING @ Fri, 10 Dec 2021 22:37:26: Fewer paired peaks (442) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 442 pairs to build model! INFO @ Fri, 10 Dec 2021 22:37:26: start model_add_line... INFO @ Fri, 10 Dec 2021 22:37:26: start X-correlation... INFO @ Fri, 10 Dec 2021 22:37:26: end of X-cor INFO @ Fri, 10 Dec 2021 22:37:26: #2 finished! INFO @ Fri, 10 Dec 2021 22:37:26: #2 predicted fragment length is 54 bps INFO @ Fri, 10 Dec 2021 22:37:26: #2 alternative fragment length(s) may be 54 bps INFO @ Fri, 10 Dec 2021 22:37:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.05_model.r WARNING @ Fri, 10 Dec 2021 22:37:26: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 22:37:26: #2 You may need to consider one of the other alternative d(s): 54 WARNING @ Fri, 10 Dec 2021 22:37:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 22:37:26: #3 Call peaks... INFO @ Fri, 10 Dec 2021 22:37:26: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 22:37:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 22:37:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 22:37:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 22:37:32: 1000000 INFO @ Fri, 10 Dec 2021 22:37:37: 2000000 INFO @ Fri, 10 Dec 2021 22:37:37: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 22:37:42: 3000000 INFO @ Fri, 10 Dec 2021 22:37:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.05_peaks.xls INFO @ Fri, 10 Dec 2021 22:37:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 22:37:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.05_summits.bed INFO @ Fri, 10 Dec 2021 22:37:43: Done! pass1 - making usageList (501 chroms): 1 millis pass2 - checking and writing primary data (1184 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 22:37:48: 4000000 INFO @ Fri, 10 Dec 2021 22:37:54: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 22:37:55: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 22:37:55: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 22:37:55: #1 total tags in treatment: 5301911 INFO @ Fri, 10 Dec 2021 22:37:55: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:37:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:37:56: #1 tags after filtering in treatment: 5301907 INFO @ Fri, 10 Dec 2021 22:37:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 22:37:56: #1 finished! INFO @ Fri, 10 Dec 2021 22:37:56: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:37:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:37:56: #2 number of paired peaks: 442 WARNING @ Fri, 10 Dec 2021 22:37:56: Fewer paired peaks (442) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 442 pairs to build model! INFO @ Fri, 10 Dec 2021 22:37:56: start model_add_line... INFO @ Fri, 10 Dec 2021 22:37:56: start X-correlation... INFO @ Fri, 10 Dec 2021 22:37:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 22:37:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 22:37:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 22:37:56: end of X-cor INFO @ Fri, 10 Dec 2021 22:37:56: #2 finished! INFO @ Fri, 10 Dec 2021 22:37:56: #2 predicted fragment length is 54 bps INFO @ Fri, 10 Dec 2021 22:37:56: #2 alternative fragment length(s) may be 54 bps INFO @ Fri, 10 Dec 2021 22:37:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.10_model.r WARNING @ Fri, 10 Dec 2021 22:37:56: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 22:37:56: #2 You may need to consider one of the other alternative d(s): 54 WARNING @ Fri, 10 Dec 2021 22:37:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 22:37:56: #3 Call peaks... INFO @ Fri, 10 Dec 2021 22:37:56: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 22:38:02: 1000000 INFO @ Fri, 10 Dec 2021 22:38:07: 2000000 INFO @ Fri, 10 Dec 2021 22:38:07: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 22:38:12: 3000000 INFO @ Fri, 10 Dec 2021 22:38:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.10_peaks.xls INFO @ Fri, 10 Dec 2021 22:38:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 22:38:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.10_summits.bed INFO @ Fri, 10 Dec 2021 22:38:13: Done! pass1 - making usageList (196 chroms): 1 millis pass2 - checking and writing primary data (414 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 22:38:18: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 22:38:23: 5000000 INFO @ Fri, 10 Dec 2021 22:38:25: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 22:38:25: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 22:38:25: #1 total tags in treatment: 5301911 INFO @ Fri, 10 Dec 2021 22:38:25: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 22:38:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 22:38:26: #1 tags after filtering in treatment: 5301907 INFO @ Fri, 10 Dec 2021 22:38:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 22:38:26: #1 finished! INFO @ Fri, 10 Dec 2021 22:38:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 22:38:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 22:38:26: #2 number of paired peaks: 442 WARNING @ Fri, 10 Dec 2021 22:38:26: Fewer paired peaks (442) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 442 pairs to build model! INFO @ Fri, 10 Dec 2021 22:38:26: start model_add_line... INFO @ Fri, 10 Dec 2021 22:38:26: start X-correlation... INFO @ Fri, 10 Dec 2021 22:38:26: end of X-cor INFO @ Fri, 10 Dec 2021 22:38:26: #2 finished! INFO @ Fri, 10 Dec 2021 22:38:26: #2 predicted fragment length is 54 bps INFO @ Fri, 10 Dec 2021 22:38:26: #2 alternative fragment length(s) may be 54 bps INFO @ Fri, 10 Dec 2021 22:38:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.20_model.r WARNING @ Fri, 10 Dec 2021 22:38:26: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 22:38:26: #2 You may need to consider one of the other alternative d(s): 54 WARNING @ Fri, 10 Dec 2021 22:38:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 22:38:26: #3 Call peaks... INFO @ Fri, 10 Dec 2021 22:38:26: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 22:38:37: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 22:38:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.20_peaks.xls INFO @ Fri, 10 Dec 2021 22:38:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 22:38:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8688989/SRX8688989.20_summits.bed INFO @ Fri, 10 Dec 2021 22:38:43: Done! pass1 - making usageList (108 chroms): 1 millis pass2 - checking and writing primary data (196 records, 4 fields): 5 millis CompletedMACS2peakCalling