Job ID = 14167828 SRX = SRX8688915 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:31 5464889 reads; of these: 5464889 (100.00%) were unpaired; of these: 182809 (3.35%) aligned 0 times 4094813 (74.93%) aligned exactly 1 time 1187267 (21.73%) aligned >1 times 96.65% overall alignment rate Time searching: 00:01:31 Overall time: 00:01:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 286817 / 5282080 = 0.0543 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:36:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:36:23: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:36:23: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:36:29: 1000000 INFO @ Fri, 10 Dec 2021 13:36:35: 2000000 INFO @ Fri, 10 Dec 2021 13:36:41: 3000000 INFO @ Fri, 10 Dec 2021 13:36:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:36:53: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 13:36:53: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 13:36:53: #1 total tags in treatment: 4995263 INFO @ Fri, 10 Dec 2021 13:36:53: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:36:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:36:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:36:53: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:36:53: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:36:53: #1 tags after filtering in treatment: 4995047 INFO @ Fri, 10 Dec 2021 13:36:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:36:53: #1 finished! INFO @ Fri, 10 Dec 2021 13:36:53: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:36:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:36:54: #2 number of paired peaks: 700 WARNING @ Fri, 10 Dec 2021 13:36:54: Fewer paired peaks (700) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 700 pairs to build model! INFO @ Fri, 10 Dec 2021 13:36:54: start model_add_line... INFO @ Fri, 10 Dec 2021 13:36:54: start X-correlation... INFO @ Fri, 10 Dec 2021 13:36:54: end of X-cor INFO @ Fri, 10 Dec 2021 13:36:54: #2 finished! INFO @ Fri, 10 Dec 2021 13:36:54: #2 predicted fragment length is 63 bps INFO @ Fri, 10 Dec 2021 13:36:54: #2 alternative fragment length(s) may be 63 bps INFO @ Fri, 10 Dec 2021 13:36:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.05_model.r WARNING @ Fri, 10 Dec 2021 13:36:54: #2 Since the d (63) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 13:36:54: #2 You may need to consider one of the other alternative d(s): 63 WARNING @ Fri, 10 Dec 2021 13:36:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 13:36:54: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:36:54: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:36:59: 1000000 INFO @ Fri, 10 Dec 2021 13:37:04: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:37:04: 2000000 INFO @ Fri, 10 Dec 2021 13:37:09: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.05_peaks.xls INFO @ Fri, 10 Dec 2021 13:37:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:37:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.05_summits.bed INFO @ Fri, 10 Dec 2021 13:37:09: Done! pass1 - making usageList (452 chroms): 1 millis pass2 - checking and writing primary data (1314 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:37:10: 3000000 INFO @ Fri, 10 Dec 2021 13:37:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:37:22: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 13:37:22: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 13:37:22: #1 total tags in treatment: 4995263 INFO @ Fri, 10 Dec 2021 13:37:22: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:37:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:37:22: #1 tags after filtering in treatment: 4995047 INFO @ Fri, 10 Dec 2021 13:37:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:37:22: #1 finished! INFO @ Fri, 10 Dec 2021 13:37:22: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:37:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:37:23: #2 number of paired peaks: 700 WARNING @ Fri, 10 Dec 2021 13:37:23: Fewer paired peaks (700) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 700 pairs to build model! INFO @ Fri, 10 Dec 2021 13:37:23: start model_add_line... INFO @ Fri, 10 Dec 2021 13:37:23: start X-correlation... INFO @ Fri, 10 Dec 2021 13:37:23: end of X-cor INFO @ Fri, 10 Dec 2021 13:37:23: #2 finished! INFO @ Fri, 10 Dec 2021 13:37:23: #2 predicted fragment length is 63 bps INFO @ Fri, 10 Dec 2021 13:37:23: #2 alternative fragment length(s) may be 63 bps INFO @ Fri, 10 Dec 2021 13:37:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.10_model.r WARNING @ Fri, 10 Dec 2021 13:37:23: #2 Since the d (63) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 13:37:23: #2 You may need to consider one of the other alternative d(s): 63 WARNING @ Fri, 10 Dec 2021 13:37:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 13:37:23: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:37:23: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:37:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:37:23: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:37:23: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:37:31: 1000000 INFO @ Fri, 10 Dec 2021 13:37:33: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:37:38: 2000000 INFO @ Fri, 10 Dec 2021 13:37:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.10_peaks.xls INFO @ Fri, 10 Dec 2021 13:37:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:37:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.10_summits.bed INFO @ Fri, 10 Dec 2021 13:37:39: Done! pass1 - making usageList (252 chroms): 1 millis pass2 - checking and writing primary data (525 records, 4 fields): 8 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:37:46: 3000000 INFO @ Fri, 10 Dec 2021 13:37:53: 4000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:38:01: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 13:38:01: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 13:38:01: #1 total tags in treatment: 4995263 INFO @ Fri, 10 Dec 2021 13:38:01: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:38:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:38:01: #1 tags after filtering in treatment: 4995047 INFO @ Fri, 10 Dec 2021 13:38:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:38:01: #1 finished! INFO @ Fri, 10 Dec 2021 13:38:01: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:38:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:38:01: #2 number of paired peaks: 700 WARNING @ Fri, 10 Dec 2021 13:38:01: Fewer paired peaks (700) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 700 pairs to build model! INFO @ Fri, 10 Dec 2021 13:38:01: start model_add_line... INFO @ Fri, 10 Dec 2021 13:38:01: start X-correlation... INFO @ Fri, 10 Dec 2021 13:38:01: end of X-cor INFO @ Fri, 10 Dec 2021 13:38:01: #2 finished! INFO @ Fri, 10 Dec 2021 13:38:01: #2 predicted fragment length is 63 bps INFO @ Fri, 10 Dec 2021 13:38:01: #2 alternative fragment length(s) may be 63 bps INFO @ Fri, 10 Dec 2021 13:38:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.20_model.r WARNING @ Fri, 10 Dec 2021 13:38:01: #2 Since the d (63) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 13:38:01: #2 You may need to consider one of the other alternative d(s): 63 WARNING @ Fri, 10 Dec 2021 13:38:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 13:38:01: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:38:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:38:12: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:38:17: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.20_peaks.xls INFO @ Fri, 10 Dec 2021 13:38:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:38:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8688915/SRX8688915.20_summits.bed INFO @ Fri, 10 Dec 2021 13:38:17: Done! pass1 - making usageList (98 chroms): 1 millis pass2 - checking and writing primary data (170 records, 4 fields): 4 millis CompletedMACS2peakCalling