Job ID = 14169547 SRX = SRX8688898 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:31 6225401 reads; of these: 6225401 (100.00%) were unpaired; of these: 352454 (5.66%) aligned 0 times 5081140 (81.62%) aligned exactly 1 time 791807 (12.72%) aligned >1 times 94.34% overall alignment rate Time searching: 00:01:31 Overall time: 00:01:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 326326 / 5872947 = 0.0556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 00:46:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 00:46:06: #1 read tag files... INFO @ Sat, 11 Dec 2021 00:46:06: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 00:46:12: 1000000 INFO @ Sat, 11 Dec 2021 00:46:19: 2000000 INFO @ Sat, 11 Dec 2021 00:46:26: 3000000 INFO @ Sat, 11 Dec 2021 00:46:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 00:46:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 00:46:36: #1 read tag files... INFO @ Sat, 11 Dec 2021 00:46:36: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 00:46:40: 5000000 INFO @ Sat, 11 Dec 2021 00:46:43: 1000000 INFO @ Sat, 11 Dec 2021 00:46:44: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 00:46:44: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 00:46:44: #1 total tags in treatment: 5546621 INFO @ Sat, 11 Dec 2021 00:46:44: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 00:46:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 00:46:45: #1 tags after filtering in treatment: 5546509 INFO @ Sat, 11 Dec 2021 00:46:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 00:46:45: #1 finished! INFO @ Sat, 11 Dec 2021 00:46:45: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 00:46:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 00:46:45: #2 number of paired peaks: 75 WARNING @ Sat, 11 Dec 2021 00:46:45: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 00:46:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 00:46:50: 2000000 INFO @ Sat, 11 Dec 2021 00:46:57: 3000000 INFO @ Sat, 11 Dec 2021 00:47:03: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 00:47:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 00:47:06: #1 read tag files... INFO @ Sat, 11 Dec 2021 00:47:06: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 00:47:11: 5000000 INFO @ Sat, 11 Dec 2021 00:47:13: 1000000 INFO @ Sat, 11 Dec 2021 00:47:15: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 00:47:15: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 00:47:15: #1 total tags in treatment: 5546621 INFO @ Sat, 11 Dec 2021 00:47:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 00:47:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 00:47:15: #1 tags after filtering in treatment: 5546509 INFO @ Sat, 11 Dec 2021 00:47:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 00:47:15: #1 finished! INFO @ Sat, 11 Dec 2021 00:47:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 00:47:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 00:47:16: #2 number of paired peaks: 75 WARNING @ Sat, 11 Dec 2021 00:47:16: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 00:47:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 00:47:19: 2000000 INFO @ Sat, 11 Dec 2021 00:47:25: 3000000 INFO @ Sat, 11 Dec 2021 00:47:31: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 00:47:37: 5000000 INFO @ Sat, 11 Dec 2021 00:47:41: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 00:47:41: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 00:47:41: #1 total tags in treatment: 5546621 INFO @ Sat, 11 Dec 2021 00:47:41: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 00:47:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 00:47:41: #1 tags after filtering in treatment: 5546509 INFO @ Sat, 11 Dec 2021 00:47:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 00:47:41: #1 finished! INFO @ Sat, 11 Dec 2021 00:47:41: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 00:47:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 00:47:41: #2 number of paired peaks: 75 WARNING @ Sat, 11 Dec 2021 00:47:41: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 00:47:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8688898/SRX8688898.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。