Job ID = 6459814 SRX = SRX859012 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:42:51 prefetch.2.10.7: 1) Downloading 'SRR1779568'... 2020-06-21T13:42:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:44:23 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:44:24 prefetch.2.10.7: 'SRR1779568' is valid 2020-06-21T13:44:24 prefetch.2.10.7: 1) 'SRR1779568' was downloaded successfully 2020-06-21T13:45:16 prefetch.2.10.7: 'SRR1779568' has 10 unresolved dependencies 2020-06-21T13:45:16 prefetch.2.10.7: 2) Downloading 'ncbi-acc:AE013599.4?vdb-ctx=refseq'... 2020-06-21T13:45:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:45:32 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:45:32 prefetch.2.10.7: 2) 'ncbi-acc:AE013599.4?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:45:32 prefetch.2.10.7: 3) Downloading 'ncbi-acc:AE014134.5?vdb-ctx=refseq'... 2020-06-21T13:45:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:45:50 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:45:50 prefetch.2.10.7: 3) 'ncbi-acc:AE014134.5?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:45:50 prefetch.2.10.7: 4) Downloading 'ncbi-acc:AE014135.3?vdb-ctx=refseq'... 2020-06-21T13:45:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:46:02 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:46:02 prefetch.2.10.7: 4) 'ncbi-acc:AE014135.3?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:46:02 prefetch.2.10.7: 5) Downloading 'ncbi-acc:AE014296.4?vdb-ctx=refseq'... 2020-06-21T13:46:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:46:20 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:46:20 prefetch.2.10.7: 5) 'ncbi-acc:AE014296.4?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:46:20 prefetch.2.10.7: 6) Downloading 'ncbi-acc:AE014297.2?vdb-ctx=refseq'... 2020-06-21T13:46:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:46:41 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:46:41 prefetch.2.10.7: 6) 'ncbi-acc:AE014297.2?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:46:41 prefetch.2.10.7: 7) Downloading 'ncbi-acc:AE014298.4?vdb-ctx=refseq'... 2020-06-21T13:46:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:46:58 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:46:58 prefetch.2.10.7: 7) 'ncbi-acc:AE014298.4?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:46:58 prefetch.2.10.7: 8) Downloading 'ncbi-acc:CM000457.1?vdb-ctx=refseq'... 2020-06-21T13:46:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:47:12 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:47:12 prefetch.2.10.7: 8) 'ncbi-acc:CM000457.1?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:47:12 prefetch.2.10.7: 9) Downloading 'ncbi-acc:CM000458.1?vdb-ctx=refseq'... 2020-06-21T13:47:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:47:24 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:47:24 prefetch.2.10.7: 9) 'ncbi-acc:CM000458.1?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:47:24 prefetch.2.10.7: 10) Downloading 'ncbi-acc:CM000459.1?vdb-ctx=refseq'... 2020-06-21T13:47:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:47:38 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:47:38 prefetch.2.10.7: 10) 'ncbi-acc:CM000459.1?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:47:38 prefetch.2.10.7: 11) Downloading 'ncbi-acc:FA000001.1?vdb-ctx=refseq'... 2020-06-21T13:47:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:47:54 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:47:54 prefetch.2.10.7: 11) 'ncbi-acc:FA000001.1?vdb-ctx=refseq' was downloaded successfully Read 15429368 spots for SRR1779568/SRR1779568.sra Written 15429368 spots for SRR1779568/SRR1779568.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:50 15429368 reads; of these: 15429368 (100.00%) were unpaired; of these: 93518 (0.61%) aligned 0 times 14721714 (95.41%) aligned exactly 1 time 614136 (3.98%) aligned >1 times 99.39% overall alignment rate Time searching: 00:02:50 Overall time: 00:02:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8058 / 15335850 = 0.0005 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:56:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:56:13: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:56:13: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:56:18: 1000000 INFO @ Sun, 21 Jun 2020 22:56:23: 2000000 INFO @ Sun, 21 Jun 2020 22:56:28: 3000000 INFO @ Sun, 21 Jun 2020 22:56:33: 4000000 INFO @ Sun, 21 Jun 2020 22:56:38: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:56:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:56:43: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:56:43: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:56:43: 6000000 INFO @ Sun, 21 Jun 2020 22:56:48: 7000000 INFO @ Sun, 21 Jun 2020 22:56:49: 1000000 INFO @ Sun, 21 Jun 2020 22:56:54: 2000000 INFO @ Sun, 21 Jun 2020 22:56:54: 8000000 INFO @ Sun, 21 Jun 2020 22:56:59: 3000000 INFO @ Sun, 21 Jun 2020 22:56:59: 9000000 INFO @ Sun, 21 Jun 2020 22:57:04: 4000000 INFO @ Sun, 21 Jun 2020 22:57:04: 10000000 INFO @ Sun, 21 Jun 2020 22:57:10: 5000000 INFO @ Sun, 21 Jun 2020 22:57:10: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:57:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:57:13: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:57:13: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:57:15: 6000000 INFO @ Sun, 21 Jun 2020 22:57:15: 12000000 INFO @ Sun, 21 Jun 2020 22:57:19: 1000000 INFO @ Sun, 21 Jun 2020 22:57:20: 7000000 INFO @ Sun, 21 Jun 2020 22:57:20: 13000000 INFO @ Sun, 21 Jun 2020 22:57:24: 2000000 INFO @ Sun, 21 Jun 2020 22:57:26: 8000000 INFO @ Sun, 21 Jun 2020 22:57:26: 14000000 INFO @ Sun, 21 Jun 2020 22:57:30: 3000000 INFO @ Sun, 21 Jun 2020 22:57:31: 9000000 INFO @ Sun, 21 Jun 2020 22:57:32: 15000000 INFO @ Sun, 21 Jun 2020 22:57:34: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:57:34: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:57:34: #1 total tags in treatment: 15327792 INFO @ Sun, 21 Jun 2020 22:57:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:57:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:57:34: #1 tags after filtering in treatment: 15327592 INFO @ Sun, 21 Jun 2020 22:57:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:57:34: #1 finished! INFO @ Sun, 21 Jun 2020 22:57:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:57:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:57:35: #2 number of paired peaks: 12 WARNING @ Sun, 21 Jun 2020 22:57:35: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:57:35: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 22:57:36: 4000000 cut: /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:57:36: 10000000 INFO @ Sun, 21 Jun 2020 22:57:42: 11000000 INFO @ Sun, 21 Jun 2020 22:57:42: 5000000 INFO @ Sun, 21 Jun 2020 22:57:47: 12000000 INFO @ Sun, 21 Jun 2020 22:57:48: 6000000 INFO @ Sun, 21 Jun 2020 22:57:53: 13000000 INFO @ Sun, 21 Jun 2020 22:57:54: 7000000 INFO @ Sun, 21 Jun 2020 22:57:58: 14000000 INFO @ Sun, 21 Jun 2020 22:58:00: 8000000 INFO @ Sun, 21 Jun 2020 22:58:04: 15000000 INFO @ Sun, 21 Jun 2020 22:58:06: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:58:06: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:58:06: #1 total tags in treatment: 15327792 INFO @ Sun, 21 Jun 2020 22:58:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:58:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:58:06: #1 tags after filtering in treatment: 15327592 INFO @ Sun, 21 Jun 2020 22:58:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:58:06: #1 finished! INFO @ Sun, 21 Jun 2020 22:58:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:58:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:58:06: 9000000 INFO @ Sun, 21 Jun 2020 22:58:07: #2 number of paired peaks: 12 WARNING @ Sun, 21 Jun 2020 22:58:07: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:58:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:58:12: 10000000 INFO @ Sun, 21 Jun 2020 22:58:18: 11000000 INFO @ Sun, 21 Jun 2020 22:58:24: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:58:30: 13000000 INFO @ Sun, 21 Jun 2020 22:58:36: 14000000 INFO @ Sun, 21 Jun 2020 22:58:42: 15000000 INFO @ Sun, 21 Jun 2020 22:58:44: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:58:44: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:58:44: #1 total tags in treatment: 15327792 INFO @ Sun, 21 Jun 2020 22:58:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:58:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:58:44: #1 tags after filtering in treatment: 15327592 INFO @ Sun, 21 Jun 2020 22:58:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:58:44: #1 finished! INFO @ Sun, 21 Jun 2020 22:58:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:58:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:58:45: #2 number of paired peaks: 12 WARNING @ Sun, 21 Jun 2020 22:58:45: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:58:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX859012/SRX859012.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。