Job ID = 6459795 SRX = SRX858996 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:30:58 prefetch.2.10.7: 1) Downloading 'SRR1779552'... 2020-06-21T13:30:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:31:49 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:31:49 prefetch.2.10.7: 'SRR1779552' is valid 2020-06-21T13:31:49 prefetch.2.10.7: 1) 'SRR1779552' was downloaded successfully 2020-06-21T13:32:41 prefetch.2.10.7: 'SRR1779552' has 10 unresolved dependencies 2020-06-21T13:32:41 prefetch.2.10.7: 2) Downloading 'ncbi-acc:AE013599.4?vdb-ctx=refseq'... 2020-06-21T13:32:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:32:58 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:32:58 prefetch.2.10.7: 2) 'ncbi-acc:AE013599.4?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:32:58 prefetch.2.10.7: 3) Downloading 'ncbi-acc:AE014134.5?vdb-ctx=refseq'... 2020-06-21T13:32:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:33:15 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:33:15 prefetch.2.10.7: 3) 'ncbi-acc:AE014134.5?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:33:15 prefetch.2.10.7: 4) Downloading 'ncbi-acc:AE014135.3?vdb-ctx=refseq'... 2020-06-21T13:33:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:33:28 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:33:28 prefetch.2.10.7: 4) 'ncbi-acc:AE014135.3?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:33:28 prefetch.2.10.7: 5) Downloading 'ncbi-acc:AE014296.4?vdb-ctx=refseq'... 2020-06-21T13:33:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:33:44 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:33:44 prefetch.2.10.7: 5) 'ncbi-acc:AE014296.4?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:33:44 prefetch.2.10.7: 6) Downloading 'ncbi-acc:AE014297.2?vdb-ctx=refseq'... 2020-06-21T13:33:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:34:02 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:34:02 prefetch.2.10.7: 6) 'ncbi-acc:AE014297.2?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:34:02 prefetch.2.10.7: 7) Downloading 'ncbi-acc:AE014298.4?vdb-ctx=refseq'... 2020-06-21T13:34:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:34:23 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:34:23 prefetch.2.10.7: 7) 'ncbi-acc:AE014298.4?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:34:23 prefetch.2.10.7: 8) Downloading 'ncbi-acc:CM000457.1?vdb-ctx=refseq'... 2020-06-21T13:34:23 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:34:36 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:34:36 prefetch.2.10.7: 8) 'ncbi-acc:CM000457.1?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:34:36 prefetch.2.10.7: 9) Downloading 'ncbi-acc:CM000458.1?vdb-ctx=refseq'... 2020-06-21T13:34:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:34:50 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:34:50 prefetch.2.10.7: 9) 'ncbi-acc:CM000458.1?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:34:50 prefetch.2.10.7: 10) Downloading 'ncbi-acc:CM000459.1?vdb-ctx=refseq'... 2020-06-21T13:34:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:35:04 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:35:04 prefetch.2.10.7: 10) 'ncbi-acc:CM000459.1?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:35:04 prefetch.2.10.7: 11) Downloading 'ncbi-acc:FA000001.1?vdb-ctx=refseq'... 2020-06-21T13:35:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:35:19 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:35:19 prefetch.2.10.7: 11) 'ncbi-acc:FA000001.1?vdb-ctx=refseq' was downloaded successfully Read 12062371 spots for SRR1779552/SRR1779552.sra Written 12062371 spots for SRR1779552/SRR1779552.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:01 12062371 reads; of these: 12062371 (100.00%) were unpaired; of these: 72620 (0.60%) aligned 0 times 11573782 (95.95%) aligned exactly 1 time 415969 (3.45%) aligned >1 times 99.40% overall alignment rate Time searching: 00:02:01 Overall time: 00:02:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4269 / 11989751 = 0.0004 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:42:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:42:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:42:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:42:37: 1000000 INFO @ Sun, 21 Jun 2020 22:42:45: 2000000 INFO @ Sun, 21 Jun 2020 22:42:52: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:42:59: 4000000 INFO @ Sun, 21 Jun 2020 22:43:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:43:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:43:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:43:07: 5000000 INFO @ Sun, 21 Jun 2020 22:43:08: 1000000 INFO @ Sun, 21 Jun 2020 22:43:14: 6000000 INFO @ Sun, 21 Jun 2020 22:43:14: 2000000 INFO @ Sun, 21 Jun 2020 22:43:21: 7000000 INFO @ Sun, 21 Jun 2020 22:43:22: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:43:28: 8000000 INFO @ Sun, 21 Jun 2020 22:43:30: 4000000 INFO @ Sun, 21 Jun 2020 22:43:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:43:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:43:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:43:36: 9000000 INFO @ Sun, 21 Jun 2020 22:43:36: 5000000 INFO @ Sun, 21 Jun 2020 22:43:37: 1000000 INFO @ Sun, 21 Jun 2020 22:43:41: 6000000 INFO @ Sun, 21 Jun 2020 22:43:43: 10000000 INFO @ Sun, 21 Jun 2020 22:43:45: 2000000 INFO @ Sun, 21 Jun 2020 22:43:47: 7000000 INFO @ Sun, 21 Jun 2020 22:43:51: 11000000 INFO @ Sun, 21 Jun 2020 22:43:52: 3000000 INFO @ Sun, 21 Jun 2020 22:43:53: 8000000 INFO @ Sun, 21 Jun 2020 22:43:58: 9000000 INFO @ Sun, 21 Jun 2020 22:43:59: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:43:59: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:43:59: #1 total tags in treatment: 11985482 INFO @ Sun, 21 Jun 2020 22:43:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:43:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:43:59: #1 tags after filtering in treatment: 11985177 INFO @ Sun, 21 Jun 2020 22:43:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:43:59: #1 finished! INFO @ Sun, 21 Jun 2020 22:43:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:43:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:44:00: 4000000 INFO @ Sun, 21 Jun 2020 22:44:00: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 22:44:00: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:44:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:44:04: 10000000 INFO @ Sun, 21 Jun 2020 22:44:07: 5000000 INFO @ Sun, 21 Jun 2020 22:44:10: 11000000 INFO @ Sun, 21 Jun 2020 22:44:14: 6000000 INFO @ Sun, 21 Jun 2020 22:44:16: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:44:16: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:44:16: #1 total tags in treatment: 11985482 INFO @ Sun, 21 Jun 2020 22:44:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:44:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:44:17: #1 tags after filtering in treatment: 11985177 INFO @ Sun, 21 Jun 2020 22:44:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:44:17: #1 finished! INFO @ Sun, 21 Jun 2020 22:44:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:44:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:44:18: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 22:44:18: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:44:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:44:22: 7000000 INFO @ Sun, 21 Jun 2020 22:44:29: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:44:36: 9000000 INFO @ Sun, 21 Jun 2020 22:44:43: 10000000 INFO @ Sun, 21 Jun 2020 22:44:51: 11000000 INFO @ Sun, 21 Jun 2020 22:44:58: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:44:58: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:44:58: #1 total tags in treatment: 11985482 INFO @ Sun, 21 Jun 2020 22:44:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:44:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:44:59: #1 tags after filtering in treatment: 11985177 INFO @ Sun, 21 Jun 2020 22:44:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:44:59: #1 finished! INFO @ Sun, 21 Jun 2020 22:44:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:44:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:44:59: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 22:44:59: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:44:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858996/SRX858996.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。