Job ID = 6459791 SRX = SRX858992 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:31:43 prefetch.2.10.7: 1) Downloading 'SRR1779548'... 2020-06-21T13:31:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:32:45 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:32:45 prefetch.2.10.7: 'SRR1779548' is valid 2020-06-21T13:32:45 prefetch.2.10.7: 1) 'SRR1779548' was downloaded successfully 2020-06-21T13:33:36 prefetch.2.10.7: 'SRR1779548' has 10 unresolved dependencies 2020-06-21T13:33:36 prefetch.2.10.7: 2) Downloading 'ncbi-acc:AE013599.4?vdb-ctx=refseq'... 2020-06-21T13:33:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:33:54 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:33:54 prefetch.2.10.7: 2) 'ncbi-acc:AE013599.4?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:33:54 prefetch.2.10.7: 3) Downloading 'ncbi-acc:AE014134.5?vdb-ctx=refseq'... 2020-06-21T13:33:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:34:16 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:34:16 prefetch.2.10.7: 3) 'ncbi-acc:AE014134.5?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:34:16 prefetch.2.10.7: 4) Downloading 'ncbi-acc:AE014135.3?vdb-ctx=refseq'... 2020-06-21T13:34:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:34:28 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:34:28 prefetch.2.10.7: 4) 'ncbi-acc:AE014135.3?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:34:28 prefetch.2.10.7: 5) Downloading 'ncbi-acc:AE014296.4?vdb-ctx=refseq'... 2020-06-21T13:34:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:34:45 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:34:45 prefetch.2.10.7: 5) 'ncbi-acc:AE014296.4?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:34:45 prefetch.2.10.7: 6) Downloading 'ncbi-acc:AE014297.2?vdb-ctx=refseq'... 2020-06-21T13:34:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:35:03 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:35:03 prefetch.2.10.7: 6) 'ncbi-acc:AE014297.2?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:35:03 prefetch.2.10.7: 7) Downloading 'ncbi-acc:AE014298.4?vdb-ctx=refseq'... 2020-06-21T13:35:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:35:21 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:35:21 prefetch.2.10.7: 7) 'ncbi-acc:AE014298.4?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:35:21 prefetch.2.10.7: 8) Downloading 'ncbi-acc:CM000457.1?vdb-ctx=refseq'... 2020-06-21T13:35:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:35:35 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:35:35 prefetch.2.10.7: 8) 'ncbi-acc:CM000457.1?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:35:35 prefetch.2.10.7: 9) Downloading 'ncbi-acc:CM000458.1?vdb-ctx=refseq'... 2020-06-21T13:35:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:35:50 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:35:50 prefetch.2.10.7: 9) 'ncbi-acc:CM000458.1?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:35:50 prefetch.2.10.7: 10) Downloading 'ncbi-acc:CM000459.1?vdb-ctx=refseq'... 2020-06-21T13:35:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:36:03 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:36:03 prefetch.2.10.7: 10) 'ncbi-acc:CM000459.1?vdb-ctx=refseq' was downloaded successfully 2020-06-21T13:36:03 prefetch.2.10.7: 11) Downloading 'ncbi-acc:FA000001.1?vdb-ctx=refseq'... 2020-06-21T13:36:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:36:19 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:36:19 prefetch.2.10.7: 11) 'ncbi-acc:FA000001.1?vdb-ctx=refseq' was downloaded successfully Read 16309411 spots for SRR1779548/SRR1779548.sra Written 16309411 spots for SRR1779548/SRR1779548.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:02 16309411 reads; of these: 16309411 (100.00%) were unpaired; of these: 279639 (1.71%) aligned 0 times 15548630 (95.34%) aligned exactly 1 time 481142 (2.95%) aligned >1 times 98.29% overall alignment rate Time searching: 00:02:02 Overall time: 00:02:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1333 / 16029772 = 0.0001 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:42:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:42:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:42:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:42:59: 1000000 INFO @ Sun, 21 Jun 2020 22:43:04: 2000000 INFO @ Sun, 21 Jun 2020 22:43:09: 3000000 INFO @ Sun, 21 Jun 2020 22:43:14: 4000000 INFO @ Sun, 21 Jun 2020 22:43:19: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:43:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:43:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:43:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:43:25: 6000000 INFO @ Sun, 21 Jun 2020 22:43:29: 1000000 INFO @ Sun, 21 Jun 2020 22:43:31: 7000000 INFO @ Sun, 21 Jun 2020 22:43:35: 2000000 INFO @ Sun, 21 Jun 2020 22:43:37: 8000000 INFO @ Sun, 21 Jun 2020 22:43:41: 3000000 INFO @ Sun, 21 Jun 2020 22:43:43: 9000000 INFO @ Sun, 21 Jun 2020 22:43:47: 4000000 INFO @ Sun, 21 Jun 2020 22:43:48: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:43:53: 5000000 INFO @ Sun, 21 Jun 2020 22:43:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:43:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:43:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:43:54: 11000000 INFO @ Sun, 21 Jun 2020 22:43:59: 6000000 INFO @ Sun, 21 Jun 2020 22:43:59: 1000000 INFO @ Sun, 21 Jun 2020 22:44:00: 12000000 INFO @ Sun, 21 Jun 2020 22:44:05: 7000000 INFO @ Sun, 21 Jun 2020 22:44:05: 2000000 INFO @ Sun, 21 Jun 2020 22:44:06: 13000000 INFO @ Sun, 21 Jun 2020 22:44:11: 8000000 INFO @ Sun, 21 Jun 2020 22:44:11: 3000000 INFO @ Sun, 21 Jun 2020 22:44:13: 14000000 INFO @ Sun, 21 Jun 2020 22:44:17: 9000000 INFO @ Sun, 21 Jun 2020 22:44:17: 4000000 INFO @ Sun, 21 Jun 2020 22:44:19: 15000000 INFO @ Sun, 21 Jun 2020 22:44:23: 10000000 INFO @ Sun, 21 Jun 2020 22:44:24: 5000000 INFO @ Sun, 21 Jun 2020 22:44:25: 16000000 INFO @ Sun, 21 Jun 2020 22:44:26: #1 tag size is determined as 34 bps INFO @ Sun, 21 Jun 2020 22:44:26: #1 tag size = 34 INFO @ Sun, 21 Jun 2020 22:44:26: #1 total tags in treatment: 16028439 INFO @ Sun, 21 Jun 2020 22:44:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:44:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:44:26: #1 tags after filtering in treatment: 16028238 INFO @ Sun, 21 Jun 2020 22:44:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:44:26: #1 finished! INFO @ Sun, 21 Jun 2020 22:44:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:44:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:44:27: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 22:44:27: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:44:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:44:29: 11000000 INFO @ Sun, 21 Jun 2020 22:44:30: 6000000 INFO @ Sun, 21 Jun 2020 22:44:35: 12000000 INFO @ Sun, 21 Jun 2020 22:44:36: 7000000 INFO @ Sun, 21 Jun 2020 22:44:41: 13000000 INFO @ Sun, 21 Jun 2020 22:44:42: 8000000 INFO @ Sun, 21 Jun 2020 22:44:48: 14000000 INFO @ Sun, 21 Jun 2020 22:44:48: 9000000 INFO @ Sun, 21 Jun 2020 22:44:54: 15000000 INFO @ Sun, 21 Jun 2020 22:44:54: 10000000 INFO @ Sun, 21 Jun 2020 22:45:00: 11000000 INFO @ Sun, 21 Jun 2020 22:45:01: 16000000 INFO @ Sun, 21 Jun 2020 22:45:01: #1 tag size is determined as 34 bps INFO @ Sun, 21 Jun 2020 22:45:01: #1 tag size = 34 INFO @ Sun, 21 Jun 2020 22:45:01: #1 total tags in treatment: 16028439 INFO @ Sun, 21 Jun 2020 22:45:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:45:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:45:02: #1 tags after filtering in treatment: 16028238 INFO @ Sun, 21 Jun 2020 22:45:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:45:02: #1 finished! INFO @ Sun, 21 Jun 2020 22:45:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:45:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:45:03: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 22:45:03: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:45:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:45:06: 12000000 INFO @ Sun, 21 Jun 2020 22:45:12: 13000000 INFO @ Sun, 21 Jun 2020 22:45:18: 14000000 INFO @ Sun, 21 Jun 2020 22:45:24: 15000000 INFO @ Sun, 21 Jun 2020 22:45:29: 16000000 INFO @ Sun, 21 Jun 2020 22:45:30: #1 tag size is determined as 34 bps INFO @ Sun, 21 Jun 2020 22:45:30: #1 tag size = 34 INFO @ Sun, 21 Jun 2020 22:45:30: #1 total tags in treatment: 16028439 INFO @ Sun, 21 Jun 2020 22:45:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:45:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:45:30: #1 tags after filtering in treatment: 16028238 INFO @ Sun, 21 Jun 2020 22:45:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:45:30: #1 finished! INFO @ Sun, 21 Jun 2020 22:45:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:45:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:45:31: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 22:45:31: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:45:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX858992/SRX858992.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。