Job ID = 14167499 SRX = SRX8574280 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:16 57863513 reads; of these: 57863513 (100.00%) were unpaired; of these: 3274458 (5.66%) aligned 0 times 40918979 (70.72%) aligned exactly 1 time 13670076 (23.62%) aligned >1 times 94.34% overall alignment rate Time searching: 00:22:16 Overall time: 00:22:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 12306050 / 54589055 = 0.2254 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:07:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:07:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:07:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:07:14: 1000000 INFO @ Fri, 10 Dec 2021 13:07:22: 2000000 INFO @ Fri, 10 Dec 2021 13:07:31: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:07:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:07:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:07:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:07:39: 4000000 INFO @ Fri, 10 Dec 2021 13:07:45: 1000000 INFO @ Fri, 10 Dec 2021 13:07:48: 5000000 INFO @ Fri, 10 Dec 2021 13:07:54: 2000000 INFO @ Fri, 10 Dec 2021 13:07:57: 6000000 INFO @ Fri, 10 Dec 2021 13:08:02: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:08:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:08:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:08:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:08:06: 7000000 INFO @ Fri, 10 Dec 2021 13:08:11: 4000000 INFO @ Fri, 10 Dec 2021 13:08:15: 8000000 INFO @ Fri, 10 Dec 2021 13:08:15: 1000000 INFO @ Fri, 10 Dec 2021 13:08:20: 5000000 INFO @ Fri, 10 Dec 2021 13:08:24: 9000000 INFO @ Fri, 10 Dec 2021 13:08:24: 2000000 INFO @ Fri, 10 Dec 2021 13:08:29: 6000000 INFO @ Fri, 10 Dec 2021 13:08:33: 10000000 INFO @ Fri, 10 Dec 2021 13:08:33: 3000000 INFO @ Fri, 10 Dec 2021 13:08:38: 7000000 INFO @ Fri, 10 Dec 2021 13:08:42: 11000000 INFO @ Fri, 10 Dec 2021 13:08:42: 4000000 INFO @ Fri, 10 Dec 2021 13:08:47: 8000000 INFO @ Fri, 10 Dec 2021 13:08:51: 12000000 INFO @ Fri, 10 Dec 2021 13:08:51: 5000000 INFO @ Fri, 10 Dec 2021 13:08:56: 9000000 INFO @ Fri, 10 Dec 2021 13:09:00: 13000000 INFO @ Fri, 10 Dec 2021 13:09:00: 6000000 INFO @ Fri, 10 Dec 2021 13:09:05: 10000000 INFO @ Fri, 10 Dec 2021 13:09:09: 14000000 INFO @ Fri, 10 Dec 2021 13:09:09: 7000000 INFO @ Fri, 10 Dec 2021 13:09:14: 11000000 INFO @ Fri, 10 Dec 2021 13:09:18: 8000000 INFO @ Fri, 10 Dec 2021 13:09:18: 15000000 INFO @ Fri, 10 Dec 2021 13:09:23: 12000000 INFO @ Fri, 10 Dec 2021 13:09:27: 9000000 INFO @ Fri, 10 Dec 2021 13:09:27: 16000000 INFO @ Fri, 10 Dec 2021 13:09:32: 13000000 INFO @ Fri, 10 Dec 2021 13:09:36: 10000000 INFO @ Fri, 10 Dec 2021 13:09:36: 17000000 INFO @ Fri, 10 Dec 2021 13:09:41: 14000000 INFO @ Fri, 10 Dec 2021 13:09:44: 11000000 INFO @ Fri, 10 Dec 2021 13:09:45: 18000000 INFO @ Fri, 10 Dec 2021 13:09:50: 15000000 INFO @ Fri, 10 Dec 2021 13:09:53: 12000000 INFO @ Fri, 10 Dec 2021 13:09:54: 19000000 INFO @ Fri, 10 Dec 2021 13:09:59: 16000000 INFO @ Fri, 10 Dec 2021 13:10:02: 13000000 INFO @ Fri, 10 Dec 2021 13:10:02: 20000000 INFO @ Fri, 10 Dec 2021 13:10:08: 17000000 INFO @ Fri, 10 Dec 2021 13:10:11: 14000000 INFO @ Fri, 10 Dec 2021 13:10:11: 21000000 INFO @ Fri, 10 Dec 2021 13:10:17: 18000000 INFO @ Fri, 10 Dec 2021 13:10:19: 15000000 INFO @ Fri, 10 Dec 2021 13:10:20: 22000000 INFO @ Fri, 10 Dec 2021 13:10:26: 19000000 INFO @ Fri, 10 Dec 2021 13:10:28: 16000000 INFO @ Fri, 10 Dec 2021 13:10:29: 23000000 INFO @ Fri, 10 Dec 2021 13:10:35: 20000000 INFO @ Fri, 10 Dec 2021 13:10:37: 17000000 INFO @ Fri, 10 Dec 2021 13:10:38: 24000000 INFO @ Fri, 10 Dec 2021 13:10:43: 21000000 INFO @ Fri, 10 Dec 2021 13:10:46: 18000000 INFO @ Fri, 10 Dec 2021 13:10:47: 25000000 INFO @ Fri, 10 Dec 2021 13:10:52: 22000000 INFO @ Fri, 10 Dec 2021 13:10:55: 19000000 INFO @ Fri, 10 Dec 2021 13:10:56: 26000000 INFO @ Fri, 10 Dec 2021 13:11:01: 23000000 INFO @ Fri, 10 Dec 2021 13:11:04: 20000000 INFO @ Fri, 10 Dec 2021 13:11:04: 27000000 INFO @ Fri, 10 Dec 2021 13:11:10: 24000000 INFO @ Fri, 10 Dec 2021 13:11:13: 21000000 INFO @ Fri, 10 Dec 2021 13:11:13: 28000000 INFO @ Fri, 10 Dec 2021 13:11:19: 25000000 INFO @ Fri, 10 Dec 2021 13:11:21: 22000000 INFO @ Fri, 10 Dec 2021 13:11:22: 29000000 INFO @ Fri, 10 Dec 2021 13:11:27: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:11:30: 23000000 INFO @ Fri, 10 Dec 2021 13:11:31: 30000000 INFO @ Fri, 10 Dec 2021 13:11:36: 27000000 INFO @ Fri, 10 Dec 2021 13:11:39: 24000000 INFO @ Fri, 10 Dec 2021 13:11:40: 31000000 INFO @ Fri, 10 Dec 2021 13:11:45: 28000000 INFO @ Fri, 10 Dec 2021 13:11:48: 25000000 INFO @ Fri, 10 Dec 2021 13:11:49: 32000000 INFO @ Fri, 10 Dec 2021 13:11:54: 29000000 INFO @ Fri, 10 Dec 2021 13:11:57: 26000000 INFO @ Fri, 10 Dec 2021 13:11:58: 33000000 INFO @ Fri, 10 Dec 2021 13:12:03: 30000000 INFO @ Fri, 10 Dec 2021 13:12:06: 27000000 INFO @ Fri, 10 Dec 2021 13:12:07: 34000000 INFO @ Fri, 10 Dec 2021 13:12:11: 31000000 INFO @ Fri, 10 Dec 2021 13:12:14: 28000000 INFO @ Fri, 10 Dec 2021 13:12:17: 35000000 INFO @ Fri, 10 Dec 2021 13:12:20: 32000000 INFO @ Fri, 10 Dec 2021 13:12:23: 29000000 INFO @ Fri, 10 Dec 2021 13:12:26: 36000000 INFO @ Fri, 10 Dec 2021 13:12:29: 33000000 INFO @ Fri, 10 Dec 2021 13:12:32: 30000000 INFO @ Fri, 10 Dec 2021 13:12:34: 37000000 INFO @ Fri, 10 Dec 2021 13:12:38: 34000000 INFO @ Fri, 10 Dec 2021 13:12:41: 31000000 INFO @ Fri, 10 Dec 2021 13:12:43: 38000000 INFO @ Fri, 10 Dec 2021 13:12:47: 35000000 INFO @ Fri, 10 Dec 2021 13:12:50: 32000000 INFO @ Fri, 10 Dec 2021 13:12:52: 39000000 INFO @ Fri, 10 Dec 2021 13:12:57: 36000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:12:59: 33000000 INFO @ Fri, 10 Dec 2021 13:13:01: 40000000 INFO @ Fri, 10 Dec 2021 13:13:06: 37000000 INFO @ Fri, 10 Dec 2021 13:13:08: 34000000 INFO @ Fri, 10 Dec 2021 13:13:10: 41000000 INFO @ Fri, 10 Dec 2021 13:13:14: 38000000 INFO @ Fri, 10 Dec 2021 13:13:17: 35000000 INFO @ Fri, 10 Dec 2021 13:13:20: 42000000 INFO @ Fri, 10 Dec 2021 13:13:23: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:13:23: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:13:23: #1 total tags in treatment: 42283005 INFO @ Fri, 10 Dec 2021 13:13:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:13:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:13:23: 39000000 INFO @ Fri, 10 Dec 2021 13:13:24: #1 tags after filtering in treatment: 42283005 INFO @ Fri, 10 Dec 2021 13:13:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:13:24: #1 finished! INFO @ Fri, 10 Dec 2021 13:13:24: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:13:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:13:26: 36000000 INFO @ Fri, 10 Dec 2021 13:13:26: #2 number of paired peaks: 82 WARNING @ Fri, 10 Dec 2021 13:13:26: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:13:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:13:32: 40000000 INFO @ Fri, 10 Dec 2021 13:13:34: 37000000 INFO @ Fri, 10 Dec 2021 13:13:40: 41000000 INFO @ Fri, 10 Dec 2021 13:13:43: 38000000 INFO @ Fri, 10 Dec 2021 13:13:48: 42000000 INFO @ Fri, 10 Dec 2021 13:13:51: 39000000 INFO @ Fri, 10 Dec 2021 13:13:51: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:13:51: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:13:51: #1 total tags in treatment: 42283005 INFO @ Fri, 10 Dec 2021 13:13:51: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:13:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:13:52: #1 tags after filtering in treatment: 42283005 INFO @ Fri, 10 Dec 2021 13:13:52: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:13:52: #1 finished! INFO @ Fri, 10 Dec 2021 13:13:52: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:13:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:13:55: #2 number of paired peaks: 82 WARNING @ Fri, 10 Dec 2021 13:13:55: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:13:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:13:58: 40000000 INFO @ Fri, 10 Dec 2021 13:14:06: 41000000 INFO @ Fri, 10 Dec 2021 13:14:14: 42000000 INFO @ Fri, 10 Dec 2021 13:14:17: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:14:17: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:14:17: #1 total tags in treatment: 42283005 INFO @ Fri, 10 Dec 2021 13:14:17: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:14:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:14:18: #1 tags after filtering in treatment: 42283005 INFO @ Fri, 10 Dec 2021 13:14:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:14:18: #1 finished! INFO @ Fri, 10 Dec 2021 13:14:18: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:14:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:14:20: #2 number of paired peaks: 82 WARNING @ Fri, 10 Dec 2021 13:14:20: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:14:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574280/SRX8574280.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling