Job ID = 14167525 SRX = SRX8574278 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:27 82931494 reads; of these: 82931494 (100.00%) were unpaired; of these: 4233902 (5.11%) aligned 0 times 58175415 (70.15%) aligned exactly 1 time 20522177 (24.75%) aligned >1 times 94.89% overall alignment rate Time searching: 00:32:27 Overall time: 00:32:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 19408727 / 78697592 = 0.2466 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:25:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:25:58: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:25:58: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:26:04: 1000000 INFO @ Fri, 10 Dec 2021 13:26:10: 2000000 INFO @ Fri, 10 Dec 2021 13:26:16: 3000000 INFO @ Fri, 10 Dec 2021 13:26:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:26:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:26:28: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:26:28: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:26:29: 5000000 INFO @ Fri, 10 Dec 2021 13:26:36: 6000000 INFO @ Fri, 10 Dec 2021 13:26:37: 1000000 INFO @ Fri, 10 Dec 2021 13:26:44: 7000000 INFO @ Fri, 10 Dec 2021 13:26:46: 2000000 INFO @ Fri, 10 Dec 2021 13:26:52: 8000000 INFO @ Fri, 10 Dec 2021 13:26:55: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:26:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:26:58: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:26:58: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:26:59: 9000000 INFO @ Fri, 10 Dec 2021 13:27:04: 4000000 INFO @ Fri, 10 Dec 2021 13:27:06: 1000000 INFO @ Fri, 10 Dec 2021 13:27:07: 10000000 INFO @ Fri, 10 Dec 2021 13:27:13: 5000000 INFO @ Fri, 10 Dec 2021 13:27:14: 2000000 INFO @ Fri, 10 Dec 2021 13:27:15: 11000000 INFO @ Fri, 10 Dec 2021 13:27:22: 6000000 INFO @ Fri, 10 Dec 2021 13:27:22: 3000000 INFO @ Fri, 10 Dec 2021 13:27:23: 12000000 INFO @ Fri, 10 Dec 2021 13:27:30: 4000000 INFO @ Fri, 10 Dec 2021 13:27:30: 7000000 INFO @ Fri, 10 Dec 2021 13:27:31: 13000000 INFO @ Fri, 10 Dec 2021 13:27:38: 5000000 INFO @ Fri, 10 Dec 2021 13:27:39: 14000000 INFO @ Fri, 10 Dec 2021 13:27:39: 8000000 INFO @ Fri, 10 Dec 2021 13:27:46: 6000000 INFO @ Fri, 10 Dec 2021 13:27:47: 15000000 INFO @ Fri, 10 Dec 2021 13:27:48: 9000000 INFO @ Fri, 10 Dec 2021 13:27:54: 7000000 INFO @ Fri, 10 Dec 2021 13:27:55: 16000000 INFO @ Fri, 10 Dec 2021 13:27:57: 10000000 INFO @ Fri, 10 Dec 2021 13:28:02: 8000000 INFO @ Fri, 10 Dec 2021 13:28:03: 17000000 INFO @ Fri, 10 Dec 2021 13:28:06: 11000000 INFO @ Fri, 10 Dec 2021 13:28:10: 9000000 INFO @ Fri, 10 Dec 2021 13:28:11: 18000000 INFO @ Fri, 10 Dec 2021 13:28:15: 12000000 INFO @ Fri, 10 Dec 2021 13:28:18: 10000000 INFO @ Fri, 10 Dec 2021 13:28:19: 19000000 INFO @ Fri, 10 Dec 2021 13:28:24: 13000000 INFO @ Fri, 10 Dec 2021 13:28:26: 11000000 INFO @ Fri, 10 Dec 2021 13:28:27: 20000000 INFO @ Fri, 10 Dec 2021 13:28:33: 14000000 INFO @ Fri, 10 Dec 2021 13:28:34: 12000000 INFO @ Fri, 10 Dec 2021 13:28:35: 21000000 INFO @ Fri, 10 Dec 2021 13:28:41: 15000000 INFO @ Fri, 10 Dec 2021 13:28:42: 13000000 INFO @ Fri, 10 Dec 2021 13:28:43: 22000000 INFO @ Fri, 10 Dec 2021 13:28:50: 16000000 INFO @ Fri, 10 Dec 2021 13:28:50: 14000000 INFO @ Fri, 10 Dec 2021 13:28:51: 23000000 INFO @ Fri, 10 Dec 2021 13:28:58: 15000000 INFO @ Fri, 10 Dec 2021 13:28:59: 24000000 INFO @ Fri, 10 Dec 2021 13:28:59: 17000000 INFO @ Fri, 10 Dec 2021 13:29:06: 16000000 INFO @ Fri, 10 Dec 2021 13:29:07: 25000000 INFO @ Fri, 10 Dec 2021 13:29:08: 18000000 INFO @ Fri, 10 Dec 2021 13:29:14: 17000000 INFO @ Fri, 10 Dec 2021 13:29:15: 26000000 INFO @ Fri, 10 Dec 2021 13:29:17: 19000000 INFO @ Fri, 10 Dec 2021 13:29:22: 18000000 INFO @ Fri, 10 Dec 2021 13:29:22: 27000000 INFO @ Fri, 10 Dec 2021 13:29:25: 20000000 INFO @ Fri, 10 Dec 2021 13:29:30: 19000000 INFO @ Fri, 10 Dec 2021 13:29:30: 28000000 INFO @ Fri, 10 Dec 2021 13:29:34: 21000000 INFO @ Fri, 10 Dec 2021 13:29:38: 20000000 INFO @ Fri, 10 Dec 2021 13:29:38: 29000000 INFO @ Fri, 10 Dec 2021 13:29:43: 22000000 INFO @ Fri, 10 Dec 2021 13:29:46: 21000000 INFO @ Fri, 10 Dec 2021 13:29:46: 30000000 INFO @ Fri, 10 Dec 2021 13:29:52: 23000000 INFO @ Fri, 10 Dec 2021 13:29:54: 22000000 INFO @ Fri, 10 Dec 2021 13:29:54: 31000000 INFO @ Fri, 10 Dec 2021 13:30:01: 24000000 INFO @ Fri, 10 Dec 2021 13:30:02: 23000000 INFO @ Fri, 10 Dec 2021 13:30:02: 32000000 INFO @ Fri, 10 Dec 2021 13:30:10: 24000000 INFO @ Fri, 10 Dec 2021 13:30:10: 25000000 INFO @ Fri, 10 Dec 2021 13:30:10: 33000000 INFO @ Fri, 10 Dec 2021 13:30:18: 25000000 INFO @ Fri, 10 Dec 2021 13:30:18: 34000000 INFO @ Fri, 10 Dec 2021 13:30:18: 26000000 INFO @ Fri, 10 Dec 2021 13:30:25: 26000000 INFO @ Fri, 10 Dec 2021 13:30:26: 35000000 INFO @ Fri, 10 Dec 2021 13:30:27: 27000000 INFO @ Fri, 10 Dec 2021 13:30:33: 27000000 INFO @ Fri, 10 Dec 2021 13:30:34: 36000000 INFO @ Fri, 10 Dec 2021 13:30:36: 28000000 INFO @ Fri, 10 Dec 2021 13:30:41: 28000000 INFO @ Fri, 10 Dec 2021 13:30:42: 37000000 INFO @ Fri, 10 Dec 2021 13:30:45: 29000000 INFO @ Fri, 10 Dec 2021 13:30:49: 29000000 INFO @ Fri, 10 Dec 2021 13:30:50: 38000000 INFO @ Fri, 10 Dec 2021 13:30:54: 30000000 INFO @ Fri, 10 Dec 2021 13:30:57: 30000000 INFO @ Fri, 10 Dec 2021 13:30:58: 39000000 INFO @ Fri, 10 Dec 2021 13:31:02: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:31:05: 31000000 INFO @ Fri, 10 Dec 2021 13:31:05: 40000000 INFO @ Fri, 10 Dec 2021 13:31:11: 32000000 INFO @ Fri, 10 Dec 2021 13:31:13: 32000000 INFO @ Fri, 10 Dec 2021 13:31:13: 41000000 INFO @ Fri, 10 Dec 2021 13:31:20: 33000000 INFO @ Fri, 10 Dec 2021 13:31:21: 33000000 INFO @ Fri, 10 Dec 2021 13:31:21: 42000000 INFO @ Fri, 10 Dec 2021 13:31:29: 43000000 INFO @ Fri, 10 Dec 2021 13:31:29: 34000000 INFO @ Fri, 10 Dec 2021 13:31:30: 34000000 INFO @ Fri, 10 Dec 2021 13:31:36: 44000000 INFO @ Fri, 10 Dec 2021 13:31:38: 35000000 INFO @ Fri, 10 Dec 2021 13:31:39: 35000000 INFO @ Fri, 10 Dec 2021 13:31:44: 45000000 INFO @ Fri, 10 Dec 2021 13:31:47: 36000000 INFO @ Fri, 10 Dec 2021 13:31:48: 36000000 INFO @ Fri, 10 Dec 2021 13:31:52: 46000000 INFO @ Fri, 10 Dec 2021 13:31:57: 37000000 INFO @ Fri, 10 Dec 2021 13:31:57: 37000000 INFO @ Fri, 10 Dec 2021 13:31:59: 47000000 INFO @ Fri, 10 Dec 2021 13:32:06: 38000000 INFO @ Fri, 10 Dec 2021 13:32:07: 38000000 INFO @ Fri, 10 Dec 2021 13:32:07: 48000000 INFO @ Fri, 10 Dec 2021 13:32:16: 49000000 INFO @ Fri, 10 Dec 2021 13:32:16: 39000000 INFO @ Fri, 10 Dec 2021 13:32:16: 39000000 INFO @ Fri, 10 Dec 2021 13:32:23: 50000000 INFO @ Fri, 10 Dec 2021 13:32:25: 40000000 INFO @ Fri, 10 Dec 2021 13:32:26: 40000000 INFO @ Fri, 10 Dec 2021 13:32:31: 51000000 INFO @ Fri, 10 Dec 2021 13:32:35: 41000000 INFO @ Fri, 10 Dec 2021 13:32:35: 41000000 INFO @ Fri, 10 Dec 2021 13:32:39: 52000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:32:45: 42000000 INFO @ Fri, 10 Dec 2021 13:32:45: 42000000 INFO @ Fri, 10 Dec 2021 13:32:46: 53000000 INFO @ Fri, 10 Dec 2021 13:32:54: 54000000 INFO @ Fri, 10 Dec 2021 13:32:55: 43000000 INFO @ Fri, 10 Dec 2021 13:32:55: 43000000 INFO @ Fri, 10 Dec 2021 13:33:02: 55000000 INFO @ Fri, 10 Dec 2021 13:33:04: 44000000 INFO @ Fri, 10 Dec 2021 13:33:05: 44000000 INFO @ Fri, 10 Dec 2021 13:33:10: 56000000 INFO @ Fri, 10 Dec 2021 13:33:13: 45000000 INFO @ Fri, 10 Dec 2021 13:33:14: 45000000 INFO @ Fri, 10 Dec 2021 13:33:18: 57000000 INFO @ Fri, 10 Dec 2021 13:33:21: 46000000 INFO @ Fri, 10 Dec 2021 13:33:23: 46000000 INFO @ Fri, 10 Dec 2021 13:33:26: 58000000 INFO @ Fri, 10 Dec 2021 13:33:29: 47000000 INFO @ Fri, 10 Dec 2021 13:33:32: 47000000 INFO @ Fri, 10 Dec 2021 13:33:34: 59000000 INFO @ Fri, 10 Dec 2021 13:33:37: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:33:37: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:33:37: #1 total tags in treatment: 59288865 INFO @ Fri, 10 Dec 2021 13:33:37: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:33:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:33:37: 48000000 INFO @ Fri, 10 Dec 2021 13:33:38: #1 tags after filtering in treatment: 59288865 INFO @ Fri, 10 Dec 2021 13:33:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:33:38: #1 finished! INFO @ Fri, 10 Dec 2021 13:33:38: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:33:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:33:41: 48000000 INFO @ Fri, 10 Dec 2021 13:33:42: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 13:33:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:33:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:33:45: 49000000 INFO @ Fri, 10 Dec 2021 13:33:50: 49000000 INFO @ Fri, 10 Dec 2021 13:33:53: 50000000 INFO @ Fri, 10 Dec 2021 13:33:59: 50000000 INFO @ Fri, 10 Dec 2021 13:34:01: 51000000 INFO @ Fri, 10 Dec 2021 13:34:08: 51000000 INFO @ Fri, 10 Dec 2021 13:34:08: 52000000 INFO @ Fri, 10 Dec 2021 13:34:16: 53000000 INFO @ Fri, 10 Dec 2021 13:34:16: 52000000 INFO @ Fri, 10 Dec 2021 13:34:24: 54000000 INFO @ Fri, 10 Dec 2021 13:34:25: 53000000 INFO @ Fri, 10 Dec 2021 13:34:31: 55000000 INFO @ Fri, 10 Dec 2021 13:34:34: 54000000 INFO @ Fri, 10 Dec 2021 13:34:39: 56000000 INFO @ Fri, 10 Dec 2021 13:34:43: 55000000 INFO @ Fri, 10 Dec 2021 13:34:47: 57000000 INFO @ Fri, 10 Dec 2021 13:34:52: 56000000 INFO @ Fri, 10 Dec 2021 13:34:54: 58000000 INFO @ Fri, 10 Dec 2021 13:35:01: 57000000 INFO @ Fri, 10 Dec 2021 13:35:02: 59000000 INFO @ Fri, 10 Dec 2021 13:35:05: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:35:05: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:35:05: #1 total tags in treatment: 59288865 INFO @ Fri, 10 Dec 2021 13:35:05: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:35:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:35:06: #1 tags after filtering in treatment: 59288865 INFO @ Fri, 10 Dec 2021 13:35:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:35:06: #1 finished! INFO @ Fri, 10 Dec 2021 13:35:06: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:35:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:35:09: 58000000 INFO @ Fri, 10 Dec 2021 13:35:10: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 13:35:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:35:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:35:17: 59000000 INFO @ Fri, 10 Dec 2021 13:35:20: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:35:20: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:35:20: #1 total tags in treatment: 59288865 INFO @ Fri, 10 Dec 2021 13:35:20: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:35:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:35:21: #1 tags after filtering in treatment: 59288865 INFO @ Fri, 10 Dec 2021 13:35:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:35:21: #1 finished! INFO @ Fri, 10 Dec 2021 13:35:21: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:35:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:35:24: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 13:35:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:35:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574278/SRX8574278.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling