Job ID = 14167505 SRX = SRX8574276 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:01 62701682 reads; of these: 62701682 (100.00%) were unpaired; of these: 3545589 (5.65%) aligned 0 times 44059188 (70.27%) aligned exactly 1 time 15096905 (24.08%) aligned >1 times 94.35% overall alignment rate Time searching: 00:22:01 Overall time: 00:22:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 12621793 / 59156093 = 0.2134 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:07:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:07:20: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:07:20: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:07:26: 1000000 INFO @ Fri, 10 Dec 2021 13:07:32: 2000000 INFO @ Fri, 10 Dec 2021 13:07:38: 3000000 INFO @ Fri, 10 Dec 2021 13:07:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:07:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:07:50: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:07:50: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:07:50: 5000000 INFO @ Fri, 10 Dec 2021 13:07:56: 1000000 INFO @ Fri, 10 Dec 2021 13:07:56: 6000000 INFO @ Fri, 10 Dec 2021 13:08:03: 2000000 INFO @ Fri, 10 Dec 2021 13:08:03: 7000000 INFO @ Fri, 10 Dec 2021 13:08:09: 3000000 INFO @ Fri, 10 Dec 2021 13:08:10: 8000000 INFO @ Fri, 10 Dec 2021 13:08:16: 4000000 INFO @ Fri, 10 Dec 2021 13:08:16: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:08:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:08:20: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:08:20: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:08:23: 5000000 INFO @ Fri, 10 Dec 2021 13:08:23: 10000000 INFO @ Fri, 10 Dec 2021 13:08:28: 1000000 INFO @ Fri, 10 Dec 2021 13:08:30: 6000000 INFO @ Fri, 10 Dec 2021 13:08:31: 11000000 INFO @ Fri, 10 Dec 2021 13:08:35: 2000000 INFO @ Fri, 10 Dec 2021 13:08:38: 7000000 INFO @ Fri, 10 Dec 2021 13:08:38: 12000000 INFO @ Fri, 10 Dec 2021 13:08:43: 3000000 INFO @ Fri, 10 Dec 2021 13:08:45: 8000000 INFO @ Fri, 10 Dec 2021 13:08:45: 13000000 INFO @ Fri, 10 Dec 2021 13:08:51: 4000000 INFO @ Fri, 10 Dec 2021 13:08:53: 9000000 INFO @ Fri, 10 Dec 2021 13:08:53: 14000000 INFO @ Fri, 10 Dec 2021 13:08:59: 5000000 INFO @ Fri, 10 Dec 2021 13:09:00: 10000000 INFO @ Fri, 10 Dec 2021 13:09:00: 15000000 INFO @ Fri, 10 Dec 2021 13:09:07: 6000000 INFO @ Fri, 10 Dec 2021 13:09:08: 11000000 INFO @ Fri, 10 Dec 2021 13:09:08: 16000000 INFO @ Fri, 10 Dec 2021 13:09:15: 7000000 INFO @ Fri, 10 Dec 2021 13:09:15: 12000000 INFO @ Fri, 10 Dec 2021 13:09:15: 17000000 INFO @ Fri, 10 Dec 2021 13:09:22: 13000000 INFO @ Fri, 10 Dec 2021 13:09:22: 18000000 INFO @ Fri, 10 Dec 2021 13:09:23: 8000000 INFO @ Fri, 10 Dec 2021 13:09:30: 14000000 INFO @ Fri, 10 Dec 2021 13:09:30: 19000000 INFO @ Fri, 10 Dec 2021 13:09:30: 9000000 INFO @ Fri, 10 Dec 2021 13:09:37: 15000000 INFO @ Fri, 10 Dec 2021 13:09:37: 20000000 INFO @ Fri, 10 Dec 2021 13:09:38: 10000000 INFO @ Fri, 10 Dec 2021 13:09:45: 16000000 INFO @ Fri, 10 Dec 2021 13:09:45: 21000000 INFO @ Fri, 10 Dec 2021 13:09:46: 11000000 INFO @ Fri, 10 Dec 2021 13:09:52: 17000000 INFO @ Fri, 10 Dec 2021 13:09:52: 22000000 INFO @ Fri, 10 Dec 2021 13:09:54: 12000000 INFO @ Fri, 10 Dec 2021 13:10:00: 18000000 INFO @ Fri, 10 Dec 2021 13:10:00: 23000000 INFO @ Fri, 10 Dec 2021 13:10:02: 13000000 INFO @ Fri, 10 Dec 2021 13:10:07: 19000000 INFO @ Fri, 10 Dec 2021 13:10:07: 24000000 INFO @ Fri, 10 Dec 2021 13:10:10: 14000000 INFO @ Fri, 10 Dec 2021 13:10:14: 20000000 INFO @ Fri, 10 Dec 2021 13:10:14: 25000000 INFO @ Fri, 10 Dec 2021 13:10:18: 15000000 INFO @ Fri, 10 Dec 2021 13:10:21: 21000000 INFO @ Fri, 10 Dec 2021 13:10:21: 26000000 INFO @ Fri, 10 Dec 2021 13:10:26: 16000000 INFO @ Fri, 10 Dec 2021 13:10:28: 22000000 INFO @ Fri, 10 Dec 2021 13:10:28: 27000000 INFO @ Fri, 10 Dec 2021 13:10:34: 17000000 INFO @ Fri, 10 Dec 2021 13:10:35: 28000000 INFO @ Fri, 10 Dec 2021 13:10:35: 23000000 INFO @ Fri, 10 Dec 2021 13:10:42: 29000000 INFO @ Fri, 10 Dec 2021 13:10:43: 24000000 INFO @ Fri, 10 Dec 2021 13:10:43: 18000000 INFO @ Fri, 10 Dec 2021 13:10:50: 30000000 INFO @ Fri, 10 Dec 2021 13:10:50: 25000000 INFO @ Fri, 10 Dec 2021 13:10:51: 19000000 INFO @ Fri, 10 Dec 2021 13:10:57: 31000000 INFO @ Fri, 10 Dec 2021 13:10:57: 26000000 INFO @ Fri, 10 Dec 2021 13:10:59: 20000000 INFO @ Fri, 10 Dec 2021 13:11:04: 32000000 INFO @ Fri, 10 Dec 2021 13:11:04: 27000000 INFO @ Fri, 10 Dec 2021 13:11:07: 21000000 INFO @ Fri, 10 Dec 2021 13:11:11: 33000000 INFO @ Fri, 10 Dec 2021 13:11:11: 28000000 INFO @ Fri, 10 Dec 2021 13:11:15: 22000000 INFO @ Fri, 10 Dec 2021 13:11:18: 34000000 INFO @ Fri, 10 Dec 2021 13:11:19: 29000000 INFO @ Fri, 10 Dec 2021 13:11:24: 23000000 INFO @ Fri, 10 Dec 2021 13:11:26: 35000000 INFO @ Fri, 10 Dec 2021 13:11:26: 30000000 INFO @ Fri, 10 Dec 2021 13:11:32: 24000000 INFO @ Fri, 10 Dec 2021 13:11:33: 36000000 INFO @ Fri, 10 Dec 2021 13:11:33: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:11:40: 25000000 INFO @ Fri, 10 Dec 2021 13:11:40: 37000000 INFO @ Fri, 10 Dec 2021 13:11:40: 32000000 INFO @ Fri, 10 Dec 2021 13:11:47: 33000000 INFO @ Fri, 10 Dec 2021 13:11:47: 38000000 INFO @ Fri, 10 Dec 2021 13:11:48: 26000000 INFO @ Fri, 10 Dec 2021 13:11:54: 34000000 INFO @ Fri, 10 Dec 2021 13:11:55: 39000000 INFO @ Fri, 10 Dec 2021 13:11:56: 27000000 INFO @ Fri, 10 Dec 2021 13:12:01: 35000000 INFO @ Fri, 10 Dec 2021 13:12:02: 40000000 INFO @ Fri, 10 Dec 2021 13:12:04: 28000000 INFO @ Fri, 10 Dec 2021 13:12:09: 36000000 INFO @ Fri, 10 Dec 2021 13:12:09: 41000000 INFO @ Fri, 10 Dec 2021 13:12:12: 29000000 INFO @ Fri, 10 Dec 2021 13:12:16: 37000000 INFO @ Fri, 10 Dec 2021 13:12:16: 42000000 INFO @ Fri, 10 Dec 2021 13:12:20: 30000000 INFO @ Fri, 10 Dec 2021 13:12:23: 38000000 INFO @ Fri, 10 Dec 2021 13:12:23: 43000000 INFO @ Fri, 10 Dec 2021 13:12:28: 31000000 INFO @ Fri, 10 Dec 2021 13:12:30: 44000000 INFO @ Fri, 10 Dec 2021 13:12:30: 39000000 INFO @ Fri, 10 Dec 2021 13:12:36: 32000000 INFO @ Fri, 10 Dec 2021 13:12:38: 40000000 INFO @ Fri, 10 Dec 2021 13:12:38: 45000000 INFO @ Fri, 10 Dec 2021 13:12:44: 33000000 INFO @ Fri, 10 Dec 2021 13:12:45: 41000000 INFO @ Fri, 10 Dec 2021 13:12:45: 46000000 INFO @ Fri, 10 Dec 2021 13:12:49: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:12:49: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:12:49: #1 total tags in treatment: 46534300 INFO @ Fri, 10 Dec 2021 13:12:49: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:12:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:12:50: #1 tags after filtering in treatment: 46534300 INFO @ Fri, 10 Dec 2021 13:12:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:12:50: #1 finished! INFO @ Fri, 10 Dec 2021 13:12:50: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:12:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:12:52: 42000000 INFO @ Fri, 10 Dec 2021 13:12:52: 34000000 INFO @ Fri, 10 Dec 2021 13:12:53: #2 number of paired peaks: 50 WARNING @ Fri, 10 Dec 2021 13:12:53: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:12:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:12:59: 43000000 INFO @ Fri, 10 Dec 2021 13:13:00: 35000000 INFO @ Fri, 10 Dec 2021 13:13:06: 44000000 INFO @ Fri, 10 Dec 2021 13:13:08: 36000000 INFO @ Fri, 10 Dec 2021 13:13:12: 45000000 INFO @ Fri, 10 Dec 2021 13:13:16: 37000000 INFO @ Fri, 10 Dec 2021 13:13:20: 46000000 INFO @ Fri, 10 Dec 2021 13:13:24: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:13:24: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:13:24: #1 total tags in treatment: 46534300 INFO @ Fri, 10 Dec 2021 13:13:24: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:13:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:13:25: #1 tags after filtering in treatment: 46534300 INFO @ Fri, 10 Dec 2021 13:13:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:13:25: #1 finished! INFO @ Fri, 10 Dec 2021 13:13:25: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:13:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:13:25: 38000000 INFO @ Fri, 10 Dec 2021 13:13:27: #2 number of paired peaks: 50 WARNING @ Fri, 10 Dec 2021 13:13:27: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:13:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:13:32: 39000000 INFO @ Fri, 10 Dec 2021 13:13:39: 40000000 INFO @ Fri, 10 Dec 2021 13:13:47: 41000000 INFO @ Fri, 10 Dec 2021 13:13:54: 42000000 INFO @ Fri, 10 Dec 2021 13:14:01: 43000000 INFO @ Fri, 10 Dec 2021 13:14:09: 44000000 INFO @ Fri, 10 Dec 2021 13:14:17: 45000000 INFO @ Fri, 10 Dec 2021 13:14:25: 46000000 INFO @ Fri, 10 Dec 2021 13:14:30: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 13:14:30: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 13:14:30: #1 total tags in treatment: 46534300 INFO @ Fri, 10 Dec 2021 13:14:30: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:14:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:14:31: #1 tags after filtering in treatment: 46534300 INFO @ Fri, 10 Dec 2021 13:14:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:14:31: #1 finished! INFO @ Fri, 10 Dec 2021 13:14:31: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:14:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:14:33: #2 number of paired peaks: 50 WARNING @ Fri, 10 Dec 2021 13:14:33: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:14:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574276/SRX8574276.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling