Job ID = 14167460 SRX = SRX8574270 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:49 68895741 reads; of these: 68895741 (100.00%) were unpaired; of these: 6028436 (8.75%) aligned 0 times 45073639 (65.42%) aligned exactly 1 time 17793666 (25.83%) aligned >1 times 91.25% overall alignment rate Time searching: 00:22:50 Overall time: 00:22:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 15535028 / 62867305 = 0.2471 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:59:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:59:39: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:59:39: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:59:49: 1000000 INFO @ Fri, 10 Dec 2021 12:59:59: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:00:09: 3000000 INFO @ Fri, 10 Dec 2021 13:00:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:00:09: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:00:09: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:00:19: 1000000 INFO @ Fri, 10 Dec 2021 13:00:19: 4000000 INFO @ Fri, 10 Dec 2021 13:00:28: 2000000 INFO @ Fri, 10 Dec 2021 13:00:29: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:00:38: 3000000 INFO @ Fri, 10 Dec 2021 13:00:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:00:39: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:00:39: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:00:40: 6000000 INFO @ Fri, 10 Dec 2021 13:00:47: 1000000 INFO @ Fri, 10 Dec 2021 13:00:48: 4000000 INFO @ Fri, 10 Dec 2021 13:00:50: 7000000 INFO @ Fri, 10 Dec 2021 13:00:55: 2000000 INFO @ Fri, 10 Dec 2021 13:00:57: 5000000 INFO @ Fri, 10 Dec 2021 13:01:00: 8000000 INFO @ Fri, 10 Dec 2021 13:01:02: 3000000 INFO @ Fri, 10 Dec 2021 13:01:07: 6000000 INFO @ Fri, 10 Dec 2021 13:01:10: 4000000 INFO @ Fri, 10 Dec 2021 13:01:11: 9000000 INFO @ Fri, 10 Dec 2021 13:01:17: 7000000 INFO @ Fri, 10 Dec 2021 13:01:18: 5000000 INFO @ Fri, 10 Dec 2021 13:01:21: 10000000 INFO @ Fri, 10 Dec 2021 13:01:25: 6000000 INFO @ Fri, 10 Dec 2021 13:01:27: 8000000 INFO @ Fri, 10 Dec 2021 13:01:32: 11000000 INFO @ Fri, 10 Dec 2021 13:01:33: 7000000 INFO @ Fri, 10 Dec 2021 13:01:37: 9000000 INFO @ Fri, 10 Dec 2021 13:01:41: 8000000 INFO @ Fri, 10 Dec 2021 13:01:42: 12000000 INFO @ Fri, 10 Dec 2021 13:01:47: 10000000 INFO @ Fri, 10 Dec 2021 13:01:48: 9000000 INFO @ Fri, 10 Dec 2021 13:01:53: 13000000 INFO @ Fri, 10 Dec 2021 13:01:56: 10000000 INFO @ Fri, 10 Dec 2021 13:01:56: 11000000 INFO @ Fri, 10 Dec 2021 13:02:03: 14000000 INFO @ Fri, 10 Dec 2021 13:02:03: 11000000 INFO @ Fri, 10 Dec 2021 13:02:06: 12000000 INFO @ Fri, 10 Dec 2021 13:02:11: 12000000 INFO @ Fri, 10 Dec 2021 13:02:14: 15000000 INFO @ Fri, 10 Dec 2021 13:02:16: 13000000 INFO @ Fri, 10 Dec 2021 13:02:19: 13000000 INFO @ Fri, 10 Dec 2021 13:02:24: 16000000 INFO @ Fri, 10 Dec 2021 13:02:26: 14000000 INFO @ Fri, 10 Dec 2021 13:02:26: 14000000 INFO @ Fri, 10 Dec 2021 13:02:34: 15000000 INFO @ Fri, 10 Dec 2021 13:02:34: 17000000 INFO @ Fri, 10 Dec 2021 13:02:36: 15000000 INFO @ Fri, 10 Dec 2021 13:02:42: 16000000 INFO @ Fri, 10 Dec 2021 13:02:45: 18000000 INFO @ Fri, 10 Dec 2021 13:02:46: 16000000 INFO @ Fri, 10 Dec 2021 13:02:49: 17000000 INFO @ Fri, 10 Dec 2021 13:02:55: 19000000 INFO @ Fri, 10 Dec 2021 13:02:56: 17000000 INFO @ Fri, 10 Dec 2021 13:02:57: 18000000 INFO @ Fri, 10 Dec 2021 13:03:04: 19000000 INFO @ Fri, 10 Dec 2021 13:03:05: 18000000 INFO @ Fri, 10 Dec 2021 13:03:06: 20000000 INFO @ Fri, 10 Dec 2021 13:03:12: 20000000 INFO @ Fri, 10 Dec 2021 13:03:15: 19000000 INFO @ Fri, 10 Dec 2021 13:03:16: 21000000 INFO @ Fri, 10 Dec 2021 13:03:20: 21000000 INFO @ Fri, 10 Dec 2021 13:03:25: 20000000 INFO @ Fri, 10 Dec 2021 13:03:27: 22000000 INFO @ Fri, 10 Dec 2021 13:03:27: 22000000 INFO @ Fri, 10 Dec 2021 13:03:35: 21000000 INFO @ Fri, 10 Dec 2021 13:03:35: 23000000 INFO @ Fri, 10 Dec 2021 13:03:37: 23000000 INFO @ Fri, 10 Dec 2021 13:03:43: 24000000 INFO @ Fri, 10 Dec 2021 13:03:45: 22000000 INFO @ Fri, 10 Dec 2021 13:03:48: 24000000 INFO @ Fri, 10 Dec 2021 13:03:51: 25000000 INFO @ Fri, 10 Dec 2021 13:03:55: 23000000 INFO @ Fri, 10 Dec 2021 13:03:58: 26000000 INFO @ Fri, 10 Dec 2021 13:03:58: 25000000 INFO @ Fri, 10 Dec 2021 13:04:04: 24000000 INFO @ Fri, 10 Dec 2021 13:04:06: 27000000 INFO @ Fri, 10 Dec 2021 13:04:09: 26000000 INFO @ Fri, 10 Dec 2021 13:04:14: 28000000 INFO @ Fri, 10 Dec 2021 13:04:14: 25000000 INFO @ Fri, 10 Dec 2021 13:04:20: 27000000 INFO @ Fri, 10 Dec 2021 13:04:21: 29000000 INFO @ Fri, 10 Dec 2021 13:04:24: 26000000 INFO @ Fri, 10 Dec 2021 13:04:29: 30000000 INFO @ Fri, 10 Dec 2021 13:04:30: 28000000 INFO @ Fri, 10 Dec 2021 13:04:34: 27000000 INFO @ Fri, 10 Dec 2021 13:04:37: 31000000 INFO @ Fri, 10 Dec 2021 13:04:41: 29000000 INFO @ Fri, 10 Dec 2021 13:04:44: 28000000 INFO @ Fri, 10 Dec 2021 13:04:44: 32000000 INFO @ Fri, 10 Dec 2021 13:04:51: 30000000 INFO @ Fri, 10 Dec 2021 13:04:52: 33000000 INFO @ Fri, 10 Dec 2021 13:04:54: 29000000 INFO @ Fri, 10 Dec 2021 13:04:59: 34000000 INFO @ Fri, 10 Dec 2021 13:05:02: 31000000 INFO @ Fri, 10 Dec 2021 13:05:04: 30000000 INFO @ Fri, 10 Dec 2021 13:05:07: 35000000 INFO @ Fri, 10 Dec 2021 13:05:12: 32000000 INFO @ Fri, 10 Dec 2021 13:05:14: 31000000 INFO @ Fri, 10 Dec 2021 13:05:15: 36000000 INFO @ Fri, 10 Dec 2021 13:05:22: 37000000 INFO @ Fri, 10 Dec 2021 13:05:23: 33000000 INFO @ Fri, 10 Dec 2021 13:05:23: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:05:30: 38000000 INFO @ Fri, 10 Dec 2021 13:05:33: 33000000 INFO @ Fri, 10 Dec 2021 13:05:33: 34000000 INFO @ Fri, 10 Dec 2021 13:05:39: 39000000 INFO @ Fri, 10 Dec 2021 13:05:43: 34000000 INFO @ Fri, 10 Dec 2021 13:05:43: 35000000 INFO @ Fri, 10 Dec 2021 13:05:46: 40000000 INFO @ Fri, 10 Dec 2021 13:05:52: 35000000 INFO @ Fri, 10 Dec 2021 13:05:54: 36000000 INFO @ Fri, 10 Dec 2021 13:05:54: 41000000 INFO @ Fri, 10 Dec 2021 13:06:02: 42000000 INFO @ Fri, 10 Dec 2021 13:06:02: 36000000 INFO @ Fri, 10 Dec 2021 13:06:04: 37000000 INFO @ Fri, 10 Dec 2021 13:06:09: 43000000 INFO @ Fri, 10 Dec 2021 13:06:12: 37000000 INFO @ Fri, 10 Dec 2021 13:06:15: 38000000 INFO @ Fri, 10 Dec 2021 13:06:17: 44000000 INFO @ Fri, 10 Dec 2021 13:06:22: 38000000 INFO @ Fri, 10 Dec 2021 13:06:25: 45000000 INFO @ Fri, 10 Dec 2021 13:06:26: 39000000 INFO @ Fri, 10 Dec 2021 13:06:32: 39000000 INFO @ Fri, 10 Dec 2021 13:06:32: 46000000 INFO @ Fri, 10 Dec 2021 13:06:36: 40000000 INFO @ Fri, 10 Dec 2021 13:06:40: 47000000 INFO @ Fri, 10 Dec 2021 13:06:42: 40000000 INFO @ Fri, 10 Dec 2021 13:06:43: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 13:06:43: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 13:06:43: #1 total tags in treatment: 47332277 INFO @ Fri, 10 Dec 2021 13:06:43: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:06:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:06:44: #1 tags after filtering in treatment: 47332277 INFO @ Fri, 10 Dec 2021 13:06:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:06:44: #1 finished! INFO @ Fri, 10 Dec 2021 13:06:44: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:06:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:06:46: 41000000 INFO @ Fri, 10 Dec 2021 13:06:48: #2 number of paired peaks: 51 WARNING @ Fri, 10 Dec 2021 13:06:48: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:06:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:06:51: 41000000 INFO @ Fri, 10 Dec 2021 13:06:57: 42000000 INFO @ Fri, 10 Dec 2021 13:07:01: 42000000 INFO @ Fri, 10 Dec 2021 13:07:07: 43000000 INFO @ Fri, 10 Dec 2021 13:07:11: 43000000 INFO @ Fri, 10 Dec 2021 13:07:17: 44000000 INFO @ Fri, 10 Dec 2021 13:07:21: 44000000 INFO @ Fri, 10 Dec 2021 13:07:28: 45000000 INFO @ Fri, 10 Dec 2021 13:07:30: 45000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:07:38: 46000000 INFO @ Fri, 10 Dec 2021 13:07:40: 46000000 INFO @ Fri, 10 Dec 2021 13:07:49: 47000000 INFO @ Fri, 10 Dec 2021 13:07:50: 47000000 INFO @ Fri, 10 Dec 2021 13:07:53: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 13:07:53: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 13:07:53: #1 total tags in treatment: 47332277 INFO @ Fri, 10 Dec 2021 13:07:53: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:07:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:07:54: #1 tags after filtering in treatment: 47332277 INFO @ Fri, 10 Dec 2021 13:07:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:07:54: #1 finished! INFO @ Fri, 10 Dec 2021 13:07:54: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:07:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:07:54: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 13:07:54: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 13:07:54: #1 total tags in treatment: 47332277 INFO @ Fri, 10 Dec 2021 13:07:54: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:07:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:07:55: #1 tags after filtering in treatment: 47332277 INFO @ Fri, 10 Dec 2021 13:07:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:07:55: #1 finished! INFO @ Fri, 10 Dec 2021 13:07:55: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:07:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:07:58: #2 number of paired peaks: 51 WARNING @ Fri, 10 Dec 2021 13:07:58: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:07:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 8 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:07:59: #2 number of paired peaks: 51 WARNING @ Fri, 10 Dec 2021 13:07:59: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:07:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574270/SRX8574270.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling