Job ID = 14167409 SRX = SRX8574264 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:22 66766333 reads; of these: 66766333 (100.00%) were unpaired; of these: 2733295 (4.09%) aligned 0 times 46834131 (70.15%) aligned exactly 1 time 17198907 (25.76%) aligned >1 times 95.91% overall alignment rate Time searching: 00:24:23 Overall time: 00:24:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 15305382 / 64033038 = 0.2390 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:51:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:51:59: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:51:59: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:52:07: 1000000 INFO @ Fri, 10 Dec 2021 12:52:14: 2000000 INFO @ Fri, 10 Dec 2021 12:52:22: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:52:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:52:29: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:52:29: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:52:29: 4000000 INFO @ Fri, 10 Dec 2021 12:52:37: 5000000 INFO @ Fri, 10 Dec 2021 12:52:37: 1000000 INFO @ Fri, 10 Dec 2021 12:52:44: 6000000 INFO @ Fri, 10 Dec 2021 12:52:46: 2000000 INFO @ Fri, 10 Dec 2021 12:52:52: 7000000 INFO @ Fri, 10 Dec 2021 12:52:54: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:52:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:52:59: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:52:59: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:53:00: 8000000 INFO @ Fri, 10 Dec 2021 12:53:03: 4000000 INFO @ Fri, 10 Dec 2021 12:53:07: 9000000 INFO @ Fri, 10 Dec 2021 12:53:08: 1000000 INFO @ Fri, 10 Dec 2021 12:53:11: 5000000 INFO @ Fri, 10 Dec 2021 12:53:15: 10000000 INFO @ Fri, 10 Dec 2021 12:53:16: 2000000 INFO @ Fri, 10 Dec 2021 12:53:19: 6000000 INFO @ Fri, 10 Dec 2021 12:53:23: 11000000 INFO @ Fri, 10 Dec 2021 12:53:25: 3000000 INFO @ Fri, 10 Dec 2021 12:53:28: 7000000 INFO @ Fri, 10 Dec 2021 12:53:30: 12000000 INFO @ Fri, 10 Dec 2021 12:53:33: 4000000 INFO @ Fri, 10 Dec 2021 12:53:36: 8000000 INFO @ Fri, 10 Dec 2021 12:53:38: 13000000 INFO @ Fri, 10 Dec 2021 12:53:42: 5000000 INFO @ Fri, 10 Dec 2021 12:53:45: 9000000 INFO @ Fri, 10 Dec 2021 12:53:46: 14000000 INFO @ Fri, 10 Dec 2021 12:53:50: 6000000 INFO @ Fri, 10 Dec 2021 12:53:53: 15000000 INFO @ Fri, 10 Dec 2021 12:53:53: 10000000 INFO @ Fri, 10 Dec 2021 12:53:59: 7000000 INFO @ Fri, 10 Dec 2021 12:54:01: 16000000 INFO @ Fri, 10 Dec 2021 12:54:02: 11000000 INFO @ Fri, 10 Dec 2021 12:54:07: 8000000 INFO @ Fri, 10 Dec 2021 12:54:09: 17000000 INFO @ Fri, 10 Dec 2021 12:54:10: 12000000 INFO @ Fri, 10 Dec 2021 12:54:16: 9000000 INFO @ Fri, 10 Dec 2021 12:54:16: 18000000 INFO @ Fri, 10 Dec 2021 12:54:18: 13000000 INFO @ Fri, 10 Dec 2021 12:54:24: 19000000 INFO @ Fri, 10 Dec 2021 12:54:24: 10000000 INFO @ Fri, 10 Dec 2021 12:54:27: 14000000 INFO @ Fri, 10 Dec 2021 12:54:32: 20000000 INFO @ Fri, 10 Dec 2021 12:54:33: 11000000 INFO @ Fri, 10 Dec 2021 12:54:35: 15000000 INFO @ Fri, 10 Dec 2021 12:54:40: 21000000 INFO @ Fri, 10 Dec 2021 12:54:41: 12000000 INFO @ Fri, 10 Dec 2021 12:54:43: 16000000 INFO @ Fri, 10 Dec 2021 12:54:48: 22000000 INFO @ Fri, 10 Dec 2021 12:54:49: 13000000 INFO @ Fri, 10 Dec 2021 12:54:51: 17000000 INFO @ Fri, 10 Dec 2021 12:54:55: 23000000 INFO @ Fri, 10 Dec 2021 12:54:57: 14000000 INFO @ Fri, 10 Dec 2021 12:55:00: 18000000 INFO @ Fri, 10 Dec 2021 12:55:03: 24000000 INFO @ Fri, 10 Dec 2021 12:55:06: 15000000 INFO @ Fri, 10 Dec 2021 12:55:08: 19000000 INFO @ Fri, 10 Dec 2021 12:55:10: 25000000 INFO @ Fri, 10 Dec 2021 12:55:14: 16000000 INFO @ Fri, 10 Dec 2021 12:55:16: 20000000 INFO @ Fri, 10 Dec 2021 12:55:18: 26000000 INFO @ Fri, 10 Dec 2021 12:55:22: 17000000 INFO @ Fri, 10 Dec 2021 12:55:25: 21000000 INFO @ Fri, 10 Dec 2021 12:55:26: 27000000 INFO @ Fri, 10 Dec 2021 12:55:31: 18000000 INFO @ Fri, 10 Dec 2021 12:55:34: 22000000 INFO @ Fri, 10 Dec 2021 12:55:34: 28000000 INFO @ Fri, 10 Dec 2021 12:55:39: 19000000 INFO @ Fri, 10 Dec 2021 12:55:41: 29000000 INFO @ Fri, 10 Dec 2021 12:55:42: 23000000 INFO @ Fri, 10 Dec 2021 12:55:48: 20000000 INFO @ Fri, 10 Dec 2021 12:55:49: 30000000 INFO @ Fri, 10 Dec 2021 12:55:50: 24000000 INFO @ Fri, 10 Dec 2021 12:55:56: 21000000 INFO @ Fri, 10 Dec 2021 12:55:56: 31000000 INFO @ Fri, 10 Dec 2021 12:55:58: 25000000 INFO @ Fri, 10 Dec 2021 12:56:04: 32000000 INFO @ Fri, 10 Dec 2021 12:56:05: 22000000 INFO @ Fri, 10 Dec 2021 12:56:06: 26000000 INFO @ Fri, 10 Dec 2021 12:56:12: 33000000 INFO @ Fri, 10 Dec 2021 12:56:13: 23000000 INFO @ Fri, 10 Dec 2021 12:56:15: 27000000 INFO @ Fri, 10 Dec 2021 12:56:19: 34000000 INFO @ Fri, 10 Dec 2021 12:56:21: 24000000 INFO @ Fri, 10 Dec 2021 12:56:23: 28000000 INFO @ Fri, 10 Dec 2021 12:56:27: 35000000 INFO @ Fri, 10 Dec 2021 12:56:29: 25000000 INFO @ Fri, 10 Dec 2021 12:56:32: 29000000 INFO @ Fri, 10 Dec 2021 12:56:35: 36000000 INFO @ Fri, 10 Dec 2021 12:56:37: 26000000 INFO @ Fri, 10 Dec 2021 12:56:40: 30000000 INFO @ Fri, 10 Dec 2021 12:56:43: 37000000 INFO @ Fri, 10 Dec 2021 12:56:46: 27000000 INFO @ Fri, 10 Dec 2021 12:56:48: 31000000 INFO @ Fri, 10 Dec 2021 12:56:50: 38000000 INFO @ Fri, 10 Dec 2021 12:56:54: 28000000 INFO @ Fri, 10 Dec 2021 12:56:56: 32000000 INFO @ Fri, 10 Dec 2021 12:56:58: 39000000 INFO @ Fri, 10 Dec 2021 12:57:02: 29000000 INFO @ Fri, 10 Dec 2021 12:57:04: 33000000 INFO @ Fri, 10 Dec 2021 12:57:06: 40000000 INFO @ Fri, 10 Dec 2021 12:57:11: 30000000 INFO @ Fri, 10 Dec 2021 12:57:13: 34000000 INFO @ Fri, 10 Dec 2021 12:57:14: 41000000 INFO @ Fri, 10 Dec 2021 12:57:19: 31000000 INFO @ Fri, 10 Dec 2021 12:57:21: 35000000 INFO @ Fri, 10 Dec 2021 12:57:21: 42000000 INFO @ Fri, 10 Dec 2021 12:57:27: 32000000 INFO @ Fri, 10 Dec 2021 12:57:29: 43000000 INFO @ Fri, 10 Dec 2021 12:57:29: 36000000 INFO @ Fri, 10 Dec 2021 12:57:35: 33000000 INFO @ Fri, 10 Dec 2021 12:57:36: 44000000 INFO @ Fri, 10 Dec 2021 12:57:37: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:57:44: 34000000 INFO @ Fri, 10 Dec 2021 12:57:44: 45000000 INFO @ Fri, 10 Dec 2021 12:57:46: 38000000 INFO @ Fri, 10 Dec 2021 12:57:52: 46000000 INFO @ Fri, 10 Dec 2021 12:57:52: 35000000 INFO @ Fri, 10 Dec 2021 12:57:54: 39000000 INFO @ Fri, 10 Dec 2021 12:57:59: 47000000 INFO @ Fri, 10 Dec 2021 12:58:00: 36000000 INFO @ Fri, 10 Dec 2021 12:58:03: 40000000 INFO @ Fri, 10 Dec 2021 12:58:07: 48000000 INFO @ Fri, 10 Dec 2021 12:58:08: 37000000 INFO @ Fri, 10 Dec 2021 12:58:11: 41000000 INFO @ Fri, 10 Dec 2021 12:58:13: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:58:13: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:58:13: #1 total tags in treatment: 48727656 INFO @ Fri, 10 Dec 2021 12:58:13: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:58:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:58:15: #1 tags after filtering in treatment: 48727656 INFO @ Fri, 10 Dec 2021 12:58:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:58:15: #1 finished! INFO @ Fri, 10 Dec 2021 12:58:15: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:58:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:58:16: 38000000 INFO @ Fri, 10 Dec 2021 12:58:19: 42000000 INFO @ Fri, 10 Dec 2021 12:58:19: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 12:58:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:58:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:58:24: 39000000 INFO @ Fri, 10 Dec 2021 12:58:27: 43000000 INFO @ Fri, 10 Dec 2021 12:58:32: 40000000 INFO @ Fri, 10 Dec 2021 12:58:35: 44000000 INFO @ Fri, 10 Dec 2021 12:58:40: 41000000 INFO @ Fri, 10 Dec 2021 12:58:43: 45000000 INFO @ Fri, 10 Dec 2021 12:58:47: 42000000 INFO @ Fri, 10 Dec 2021 12:58:51: 46000000 INFO @ Fri, 10 Dec 2021 12:58:56: 43000000 INFO @ Fri, 10 Dec 2021 12:58:59: 47000000 INFO @ Fri, 10 Dec 2021 12:59:04: 44000000 INFO @ Fri, 10 Dec 2021 12:59:07: 48000000 INFO @ Fri, 10 Dec 2021 12:59:12: 45000000 INFO @ Fri, 10 Dec 2021 12:59:14: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:59:14: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:59:14: #1 total tags in treatment: 48727656 INFO @ Fri, 10 Dec 2021 12:59:14: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:59:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:59:15: #1 tags after filtering in treatment: 48727656 INFO @ Fri, 10 Dec 2021 12:59:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:59:15: #1 finished! INFO @ Fri, 10 Dec 2021 12:59:15: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:59:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:59:19: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 12:59:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:59:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:59:20: 46000000 INFO @ Fri, 10 Dec 2021 12:59:28: 47000000 INFO @ Fri, 10 Dec 2021 12:59:36: 48000000 INFO @ Fri, 10 Dec 2021 12:59:42: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:59:42: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:59:42: #1 total tags in treatment: 48727656 INFO @ Fri, 10 Dec 2021 12:59:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:59:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:59:43: #1 tags after filtering in treatment: 48727656 INFO @ Fri, 10 Dec 2021 12:59:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:59:43: #1 finished! INFO @ Fri, 10 Dec 2021 12:59:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:59:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:59:47: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 12:59:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:59:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8574264/SRX8574264.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。