Job ID = 14168006 SRX = SRX8491177 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:22 22323781 reads; of these: 22323781 (100.00%) were unpaired; of these: 17835363 (79.89%) aligned 0 times 4486354 (20.10%) aligned exactly 1 time 2064 (0.01%) aligned >1 times 20.11% overall alignment rate Time searching: 00:02:22 Overall time: 00:02:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 841073 / 4488418 = 0.1874 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:20:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:20:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:20:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:20:23: 1000000 INFO @ Fri, 10 Dec 2021 14:20:29: 2000000 INFO @ Fri, 10 Dec 2021 14:20:36: 3000000 INFO @ Fri, 10 Dec 2021 14:20:40: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 14:20:40: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 14:20:40: #1 total tags in treatment: 3647345 INFO @ Fri, 10 Dec 2021 14:20:40: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:20:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:20:40: #1 tags after filtering in treatment: 3647178 INFO @ Fri, 10 Dec 2021 14:20:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 14:20:40: #1 finished! INFO @ Fri, 10 Dec 2021 14:20:40: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:20:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:20:41: #2 number of paired peaks: 1656 INFO @ Fri, 10 Dec 2021 14:20:41: start model_add_line... INFO @ Fri, 10 Dec 2021 14:20:41: start X-correlation... INFO @ Fri, 10 Dec 2021 14:20:41: end of X-cor INFO @ Fri, 10 Dec 2021 14:20:41: #2 finished! INFO @ Fri, 10 Dec 2021 14:20:41: #2 predicted fragment length is 172 bps INFO @ Fri, 10 Dec 2021 14:20:41: #2 alternative fragment length(s) may be 3,172 bps INFO @ Fri, 10 Dec 2021 14:20:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.05_model.r INFO @ Fri, 10 Dec 2021 14:20:41: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:20:41: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:20:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:20:46: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:20:46: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:20:50: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:20:54: 1000000 INFO @ Fri, 10 Dec 2021 14:20:55: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.05_peaks.xls INFO @ Fri, 10 Dec 2021 14:20:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:20:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.05_summits.bed INFO @ Fri, 10 Dec 2021 14:20:55: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (198 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 14:21:03: 2000000 INFO @ Fri, 10 Dec 2021 14:21:11: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 14:21:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 14:21:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 14:21:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 14:21:17: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 14:21:17: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 14:21:17: #1 total tags in treatment: 3647345 INFO @ Fri, 10 Dec 2021 14:21:17: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:21:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:21:17: #1 tags after filtering in treatment: 3647178 INFO @ Fri, 10 Dec 2021 14:21:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 14:21:17: #1 finished! INFO @ Fri, 10 Dec 2021 14:21:17: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:21:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:21:17: #2 number of paired peaks: 1656 INFO @ Fri, 10 Dec 2021 14:21:17: start model_add_line... INFO @ Fri, 10 Dec 2021 14:21:17: start X-correlation... INFO @ Fri, 10 Dec 2021 14:21:17: end of X-cor INFO @ Fri, 10 Dec 2021 14:21:17: #2 finished! INFO @ Fri, 10 Dec 2021 14:21:17: #2 predicted fragment length is 172 bps INFO @ Fri, 10 Dec 2021 14:21:17: #2 alternative fragment length(s) may be 3,172 bps INFO @ Fri, 10 Dec 2021 14:21:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.10_model.r INFO @ Fri, 10 Dec 2021 14:21:17: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:21:17: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 14:21:23: 1000000 INFO @ Fri, 10 Dec 2021 14:21:28: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:21:30: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 14:21:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.10_peaks.xls INFO @ Fri, 10 Dec 2021 14:21:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:21:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.10_summits.bed INFO @ Fri, 10 Dec 2021 14:21:34: Done! pass1 - making usageList (4 chroms): 0 millis pass2 - checking and writing primary data (5 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 14:21:37: 3000000 INFO @ Fri, 10 Dec 2021 14:21:41: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 14:21:41: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 14:21:41: #1 total tags in treatment: 3647345 INFO @ Fri, 10 Dec 2021 14:21:41: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 14:21:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 14:21:41: #1 tags after filtering in treatment: 3647178 INFO @ Fri, 10 Dec 2021 14:21:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 14:21:41: #1 finished! INFO @ Fri, 10 Dec 2021 14:21:41: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 14:21:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 14:21:41: #2 number of paired peaks: 1656 INFO @ Fri, 10 Dec 2021 14:21:41: start model_add_line... INFO @ Fri, 10 Dec 2021 14:21:41: start X-correlation... INFO @ Fri, 10 Dec 2021 14:21:41: end of X-cor INFO @ Fri, 10 Dec 2021 14:21:41: #2 finished! INFO @ Fri, 10 Dec 2021 14:21:41: #2 predicted fragment length is 172 bps INFO @ Fri, 10 Dec 2021 14:21:41: #2 alternative fragment length(s) may be 3,172 bps INFO @ Fri, 10 Dec 2021 14:21:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.20_model.r INFO @ Fri, 10 Dec 2021 14:21:41: #3 Call peaks... INFO @ Fri, 10 Dec 2021 14:21:41: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 14:21:51: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 14:21:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.20_peaks.xls INFO @ Fri, 10 Dec 2021 14:21:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 14:21:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX8491177/SRX8491177.20_summits.bed INFO @ Fri, 10 Dec 2021 14:21:56: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (1 records, 4 fields): 1 millis CompletedMACS2peakCalling