Job ID = 16440061 SRX = SRX8369899 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:37 77462355 reads; of these: 77462355 (100.00%) were unpaired; of these: 3163233 (4.08%) aligned 0 times 55794637 (72.03%) aligned exactly 1 time 18504485 (23.89%) aligned >1 times 95.92% overall alignment rate Time searching: 00:22:37 Overall time: 00:22:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 19623952 / 74299122 = 0.2641 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:25:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:25:20: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:25:20: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:25:26: 1000000 INFO @ Tue, 02 Aug 2022 17:25:33: 2000000 INFO @ Tue, 02 Aug 2022 17:25:40: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:25:46: 4000000 INFO @ Tue, 02 Aug 2022 17:25:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:25:49: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:25:49: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:25:54: 5000000 INFO @ Tue, 02 Aug 2022 17:25:56: 1000000 INFO @ Tue, 02 Aug 2022 17:26:03: 6000000 INFO @ Tue, 02 Aug 2022 17:26:03: 2000000 INFO @ Tue, 02 Aug 2022 17:26:10: 7000000 INFO @ Tue, 02 Aug 2022 17:26:11: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:26:17: 8000000 INFO @ Tue, 02 Aug 2022 17:26:18: 4000000 INFO @ Tue, 02 Aug 2022 17:26:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:26:18: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:26:18: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:26:25: 9000000 INFO @ Tue, 02 Aug 2022 17:26:25: 5000000 INFO @ Tue, 02 Aug 2022 17:26:25: 1000000 INFO @ Tue, 02 Aug 2022 17:26:32: 10000000 INFO @ Tue, 02 Aug 2022 17:26:32: 2000000 INFO @ Tue, 02 Aug 2022 17:26:33: 6000000 INFO @ Tue, 02 Aug 2022 17:26:39: 11000000 INFO @ Tue, 02 Aug 2022 17:26:39: 3000000 INFO @ Tue, 02 Aug 2022 17:26:40: 7000000 INFO @ Tue, 02 Aug 2022 17:26:47: 4000000 INFO @ Tue, 02 Aug 2022 17:26:47: 12000000 INFO @ Tue, 02 Aug 2022 17:26:47: 8000000 INFO @ Tue, 02 Aug 2022 17:26:54: 5000000 INFO @ Tue, 02 Aug 2022 17:26:54: 9000000 INFO @ Tue, 02 Aug 2022 17:26:55: 13000000 INFO @ Tue, 02 Aug 2022 17:27:01: 6000000 INFO @ Tue, 02 Aug 2022 17:27:02: 10000000 INFO @ Tue, 02 Aug 2022 17:27:03: 14000000 INFO @ Tue, 02 Aug 2022 17:27:08: 7000000 INFO @ Tue, 02 Aug 2022 17:27:09: 11000000 INFO @ Tue, 02 Aug 2022 17:27:11: 15000000 INFO @ Tue, 02 Aug 2022 17:27:15: 8000000 INFO @ Tue, 02 Aug 2022 17:27:17: 12000000 INFO @ Tue, 02 Aug 2022 17:27:19: 16000000 INFO @ Tue, 02 Aug 2022 17:27:22: 9000000 INFO @ Tue, 02 Aug 2022 17:27:24: 13000000 INFO @ Tue, 02 Aug 2022 17:27:27: 17000000 INFO @ Tue, 02 Aug 2022 17:27:29: 10000000 INFO @ Tue, 02 Aug 2022 17:27:31: 14000000 INFO @ Tue, 02 Aug 2022 17:27:35: 18000000 INFO @ Tue, 02 Aug 2022 17:27:36: 11000000 INFO @ Tue, 02 Aug 2022 17:27:38: 15000000 INFO @ Tue, 02 Aug 2022 17:27:43: 19000000 INFO @ Tue, 02 Aug 2022 17:27:43: 12000000 INFO @ Tue, 02 Aug 2022 17:27:46: 16000000 INFO @ Tue, 02 Aug 2022 17:27:50: 20000000 INFO @ Tue, 02 Aug 2022 17:27:50: 13000000 INFO @ Tue, 02 Aug 2022 17:27:53: 17000000 INFO @ Tue, 02 Aug 2022 17:27:57: 14000000 INFO @ Tue, 02 Aug 2022 17:27:58: 21000000 INFO @ Tue, 02 Aug 2022 17:28:01: 18000000 INFO @ Tue, 02 Aug 2022 17:28:05: 15000000 INFO @ Tue, 02 Aug 2022 17:28:06: 22000000 INFO @ Tue, 02 Aug 2022 17:28:08: 19000000 INFO @ Tue, 02 Aug 2022 17:28:12: 16000000 INFO @ Tue, 02 Aug 2022 17:28:13: 23000000 INFO @ Tue, 02 Aug 2022 17:28:15: 20000000 INFO @ Tue, 02 Aug 2022 17:28:19: 17000000 INFO @ Tue, 02 Aug 2022 17:28:21: 24000000 INFO @ Tue, 02 Aug 2022 17:28:22: 21000000 INFO @ Tue, 02 Aug 2022 17:28:26: 18000000 INFO @ Tue, 02 Aug 2022 17:28:29: 25000000 INFO @ Tue, 02 Aug 2022 17:28:30: 22000000 INFO @ Tue, 02 Aug 2022 17:28:33: 19000000 INFO @ Tue, 02 Aug 2022 17:28:36: 26000000 INFO @ Tue, 02 Aug 2022 17:28:37: 23000000 INFO @ Tue, 02 Aug 2022 17:28:40: 20000000 INFO @ Tue, 02 Aug 2022 17:28:44: 27000000 INFO @ Tue, 02 Aug 2022 17:28:45: 24000000 INFO @ Tue, 02 Aug 2022 17:28:47: 21000000 INFO @ Tue, 02 Aug 2022 17:28:52: 28000000 INFO @ Tue, 02 Aug 2022 17:28:52: 25000000 INFO @ Tue, 02 Aug 2022 17:28:54: 22000000 INFO @ Tue, 02 Aug 2022 17:28:59: 29000000 INFO @ Tue, 02 Aug 2022 17:28:59: 26000000 INFO @ Tue, 02 Aug 2022 17:29:01: 23000000 INFO @ Tue, 02 Aug 2022 17:29:06: 27000000 INFO @ Tue, 02 Aug 2022 17:29:07: 30000000 INFO @ Tue, 02 Aug 2022 17:29:09: 24000000 INFO @ Tue, 02 Aug 2022 17:29:14: 28000000 INFO @ Tue, 02 Aug 2022 17:29:15: 31000000 INFO @ Tue, 02 Aug 2022 17:29:16: 25000000 INFO @ Tue, 02 Aug 2022 17:29:21: 29000000 INFO @ Tue, 02 Aug 2022 17:29:22: 32000000 INFO @ Tue, 02 Aug 2022 17:29:23: 26000000 INFO @ Tue, 02 Aug 2022 17:29:28: 30000000 INFO @ Tue, 02 Aug 2022 17:29:30: 33000000 INFO @ Tue, 02 Aug 2022 17:29:30: 27000000 INFO @ Tue, 02 Aug 2022 17:29:35: 31000000 INFO @ Tue, 02 Aug 2022 17:29:37: 28000000 INFO @ Tue, 02 Aug 2022 17:29:37: 34000000 INFO @ Tue, 02 Aug 2022 17:29:43: 32000000 INFO @ Tue, 02 Aug 2022 17:29:44: 29000000 INFO @ Tue, 02 Aug 2022 17:29:45: 35000000 INFO @ Tue, 02 Aug 2022 17:29:51: 33000000 INFO @ Tue, 02 Aug 2022 17:29:51: 30000000 INFO @ Tue, 02 Aug 2022 17:29:53: 36000000 INFO @ Tue, 02 Aug 2022 17:29:59: 34000000 INFO @ Tue, 02 Aug 2022 17:29:59: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:30:01: 37000000 INFO @ Tue, 02 Aug 2022 17:30:06: 32000000 INFO @ Tue, 02 Aug 2022 17:30:06: 35000000 INFO @ Tue, 02 Aug 2022 17:30:09: 38000000 INFO @ Tue, 02 Aug 2022 17:30:13: 33000000 INFO @ Tue, 02 Aug 2022 17:30:14: 36000000 INFO @ Tue, 02 Aug 2022 17:30:17: 39000000 INFO @ Tue, 02 Aug 2022 17:30:20: 34000000 INFO @ Tue, 02 Aug 2022 17:30:21: 37000000 INFO @ Tue, 02 Aug 2022 17:30:25: 40000000 INFO @ Tue, 02 Aug 2022 17:30:28: 35000000 INFO @ Tue, 02 Aug 2022 17:30:29: 38000000 INFO @ Tue, 02 Aug 2022 17:30:32: 41000000 INFO @ Tue, 02 Aug 2022 17:30:35: 36000000 INFO @ Tue, 02 Aug 2022 17:30:36: 39000000 INFO @ Tue, 02 Aug 2022 17:30:40: 42000000 INFO @ Tue, 02 Aug 2022 17:30:42: 37000000 INFO @ Tue, 02 Aug 2022 17:30:44: 40000000 INFO @ Tue, 02 Aug 2022 17:30:48: 43000000 INFO @ Tue, 02 Aug 2022 17:30:49: 38000000 INFO @ Tue, 02 Aug 2022 17:30:51: 41000000 INFO @ Tue, 02 Aug 2022 17:30:56: 39000000 INFO @ Tue, 02 Aug 2022 17:30:56: 44000000 INFO @ Tue, 02 Aug 2022 17:30:59: 42000000 INFO @ Tue, 02 Aug 2022 17:31:04: 40000000 INFO @ Tue, 02 Aug 2022 17:31:05: 45000000 INFO @ Tue, 02 Aug 2022 17:31:06: 43000000 INFO @ Tue, 02 Aug 2022 17:31:12: 41000000 INFO @ Tue, 02 Aug 2022 17:31:12: 46000000 INFO @ Tue, 02 Aug 2022 17:31:14: 44000000 INFO @ Tue, 02 Aug 2022 17:31:20: 47000000 INFO @ Tue, 02 Aug 2022 17:31:20: 42000000 INFO @ Tue, 02 Aug 2022 17:31:22: 45000000 INFO @ Tue, 02 Aug 2022 17:31:27: 48000000 INFO @ Tue, 02 Aug 2022 17:31:28: 43000000 INFO @ Tue, 02 Aug 2022 17:31:29: 46000000 INFO @ Tue, 02 Aug 2022 17:31:35: 49000000 INFO @ Tue, 02 Aug 2022 17:31:36: 44000000 INFO @ Tue, 02 Aug 2022 17:31:36: 47000000 INFO @ Tue, 02 Aug 2022 17:31:42: 50000000 INFO @ Tue, 02 Aug 2022 17:31:43: 48000000 INFO @ Tue, 02 Aug 2022 17:31:44: 45000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 17:31:49: 51000000 INFO @ Tue, 02 Aug 2022 17:31:51: 49000000 INFO @ Tue, 02 Aug 2022 17:31:53: 46000000 INFO @ Tue, 02 Aug 2022 17:31:57: 52000000 INFO @ Tue, 02 Aug 2022 17:31:58: 50000000 INFO @ Tue, 02 Aug 2022 17:32:01: 47000000 INFO @ Tue, 02 Aug 2022 17:32:04: 53000000 INFO @ Tue, 02 Aug 2022 17:32:05: 51000000 INFO @ Tue, 02 Aug 2022 17:32:09: 48000000 INFO @ Tue, 02 Aug 2022 17:32:12: 54000000 INFO @ Tue, 02 Aug 2022 17:32:12: 52000000 INFO @ Tue, 02 Aug 2022 17:32:16: 49000000 INFO @ Tue, 02 Aug 2022 17:32:17: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:32:17: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:32:17: #1 total tags in treatment: 54675170 INFO @ Tue, 02 Aug 2022 17:32:17: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:32:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:32:19: #1 tags after filtering in treatment: 54675170 INFO @ Tue, 02 Aug 2022 17:32:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:32:19: #1 finished! INFO @ Tue, 02 Aug 2022 17:32:19: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:32:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:32:20: 53000000 INFO @ Tue, 02 Aug 2022 17:32:23: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 17:32:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:32:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:32:24: 50000000 INFO @ Tue, 02 Aug 2022 17:32:27: 54000000 INFO @ Tue, 02 Aug 2022 17:32:31: 51000000 INFO @ Tue, 02 Aug 2022 17:32:32: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:32:32: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:32:32: #1 total tags in treatment: 54675170 INFO @ Tue, 02 Aug 2022 17:32:32: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:32:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:32:34: #1 tags after filtering in treatment: 54675170 INFO @ Tue, 02 Aug 2022 17:32:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:32:34: #1 finished! INFO @ Tue, 02 Aug 2022 17:32:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:32:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:32:37: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 17:32:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:32:37: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 17:32:38: 52000000 cut: /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:32:45: 53000000 INFO @ Tue, 02 Aug 2022 17:32:52: 54000000 INFO @ Tue, 02 Aug 2022 17:32:57: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:32:57: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:32:57: #1 total tags in treatment: 54675170 INFO @ Tue, 02 Aug 2022 17:32:57: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:32:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:32:58: #1 tags after filtering in treatment: 54675170 INFO @ Tue, 02 Aug 2022 17:32:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:32:58: #1 finished! INFO @ Tue, 02 Aug 2022 17:32:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:32:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:33:02: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 17:33:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:33:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369899/SRX8369899.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling