Job ID = 16440090 SRX = SRX8369897 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:33 96890763 reads; of these: 96890763 (100.00%) were unpaired; of these: 8659273 (8.94%) aligned 0 times 64712702 (66.79%) aligned exactly 1 time 23518788 (24.27%) aligned >1 times 91.06% overall alignment rate Time searching: 00:25:33 Overall time: 00:25:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 27207225 / 88231490 = 0.3084 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:45:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:45:11: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:45:11: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:45:17: 1000000 INFO @ Tue, 02 Aug 2022 17:45:23: 2000000 INFO @ Tue, 02 Aug 2022 17:45:28: 3000000 INFO @ Tue, 02 Aug 2022 17:45:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:45:39: 5000000 INFO @ Tue, 02 Aug 2022 17:45:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:45:40: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:45:40: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:45:45: 6000000 INFO @ Tue, 02 Aug 2022 17:45:47: 1000000 INFO @ Tue, 02 Aug 2022 17:45:51: 7000000 INFO @ Tue, 02 Aug 2022 17:45:53: 2000000 INFO @ Tue, 02 Aug 2022 17:45:58: 8000000 INFO @ Tue, 02 Aug 2022 17:46:00: 3000000 INFO @ Tue, 02 Aug 2022 17:46:04: 9000000 INFO @ Tue, 02 Aug 2022 17:46:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:46:10: 10000000 INFO @ Tue, 02 Aug 2022 17:46:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:46:10: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:46:10: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:46:13: 5000000 INFO @ Tue, 02 Aug 2022 17:46:16: 11000000 INFO @ Tue, 02 Aug 2022 17:46:17: 1000000 INFO @ Tue, 02 Aug 2022 17:46:19: 6000000 INFO @ Tue, 02 Aug 2022 17:46:22: 12000000 INFO @ Tue, 02 Aug 2022 17:46:23: 2000000 INFO @ Tue, 02 Aug 2022 17:46:26: 7000000 INFO @ Tue, 02 Aug 2022 17:46:29: 13000000 INFO @ Tue, 02 Aug 2022 17:46:29: 3000000 INFO @ Tue, 02 Aug 2022 17:46:33: 8000000 INFO @ Tue, 02 Aug 2022 17:46:35: 14000000 INFO @ Tue, 02 Aug 2022 17:46:35: 4000000 INFO @ Tue, 02 Aug 2022 17:46:39: 9000000 INFO @ Tue, 02 Aug 2022 17:46:41: 15000000 INFO @ Tue, 02 Aug 2022 17:46:41: 5000000 INFO @ Tue, 02 Aug 2022 17:46:46: 10000000 INFO @ Tue, 02 Aug 2022 17:46:47: 16000000 INFO @ Tue, 02 Aug 2022 17:46:48: 6000000 INFO @ Tue, 02 Aug 2022 17:46:52: 11000000 INFO @ Tue, 02 Aug 2022 17:46:54: 17000000 INFO @ Tue, 02 Aug 2022 17:46:54: 7000000 INFO @ Tue, 02 Aug 2022 17:46:59: 12000000 INFO @ Tue, 02 Aug 2022 17:47:00: 18000000 INFO @ Tue, 02 Aug 2022 17:47:00: 8000000 INFO @ Tue, 02 Aug 2022 17:47:05: 13000000 INFO @ Tue, 02 Aug 2022 17:47:06: 9000000 INFO @ Tue, 02 Aug 2022 17:47:07: 19000000 INFO @ Tue, 02 Aug 2022 17:47:12: 14000000 INFO @ Tue, 02 Aug 2022 17:47:13: 10000000 INFO @ Tue, 02 Aug 2022 17:47:13: 20000000 INFO @ Tue, 02 Aug 2022 17:47:19: 15000000 INFO @ Tue, 02 Aug 2022 17:47:19: 11000000 INFO @ Tue, 02 Aug 2022 17:47:20: 21000000 INFO @ Tue, 02 Aug 2022 17:47:25: 16000000 INFO @ Tue, 02 Aug 2022 17:47:25: 12000000 INFO @ Tue, 02 Aug 2022 17:47:27: 22000000 INFO @ Tue, 02 Aug 2022 17:47:32: 13000000 INFO @ Tue, 02 Aug 2022 17:47:32: 17000000 INFO @ Tue, 02 Aug 2022 17:47:33: 23000000 INFO @ Tue, 02 Aug 2022 17:47:38: 14000000 INFO @ Tue, 02 Aug 2022 17:47:39: 18000000 INFO @ Tue, 02 Aug 2022 17:47:40: 24000000 INFO @ Tue, 02 Aug 2022 17:47:44: 15000000 INFO @ Tue, 02 Aug 2022 17:47:46: 19000000 INFO @ Tue, 02 Aug 2022 17:47:47: 25000000 INFO @ Tue, 02 Aug 2022 17:47:50: 16000000 INFO @ Tue, 02 Aug 2022 17:47:53: 20000000 INFO @ Tue, 02 Aug 2022 17:47:53: 26000000 INFO @ Tue, 02 Aug 2022 17:47:57: 17000000 INFO @ Tue, 02 Aug 2022 17:48:00: 21000000 INFO @ Tue, 02 Aug 2022 17:48:00: 27000000 INFO @ Tue, 02 Aug 2022 17:48:03: 18000000 INFO @ Tue, 02 Aug 2022 17:48:07: 22000000 INFO @ Tue, 02 Aug 2022 17:48:07: 28000000 INFO @ Tue, 02 Aug 2022 17:48:10: 19000000 INFO @ Tue, 02 Aug 2022 17:48:13: 29000000 INFO @ Tue, 02 Aug 2022 17:48:14: 23000000 INFO @ Tue, 02 Aug 2022 17:48:16: 20000000 INFO @ Tue, 02 Aug 2022 17:48:20: 30000000 INFO @ Tue, 02 Aug 2022 17:48:21: 24000000 INFO @ Tue, 02 Aug 2022 17:48:23: 21000000 INFO @ Tue, 02 Aug 2022 17:48:27: 31000000 INFO @ Tue, 02 Aug 2022 17:48:28: 25000000 INFO @ Tue, 02 Aug 2022 17:48:30: 22000000 INFO @ Tue, 02 Aug 2022 17:48:33: 32000000 INFO @ Tue, 02 Aug 2022 17:48:35: 26000000 INFO @ Tue, 02 Aug 2022 17:48:36: 23000000 INFO @ Tue, 02 Aug 2022 17:48:40: 33000000 INFO @ Tue, 02 Aug 2022 17:48:41: 27000000 INFO @ Tue, 02 Aug 2022 17:48:43: 24000000 INFO @ Tue, 02 Aug 2022 17:48:47: 34000000 INFO @ Tue, 02 Aug 2022 17:48:48: 28000000 INFO @ Tue, 02 Aug 2022 17:48:50: 25000000 INFO @ Tue, 02 Aug 2022 17:48:54: 35000000 INFO @ Tue, 02 Aug 2022 17:48:55: 29000000 INFO @ Tue, 02 Aug 2022 17:48:56: 26000000 INFO @ Tue, 02 Aug 2022 17:49:00: 36000000 INFO @ Tue, 02 Aug 2022 17:49:02: 30000000 INFO @ Tue, 02 Aug 2022 17:49:03: 27000000 INFO @ Tue, 02 Aug 2022 17:49:07: 37000000 INFO @ Tue, 02 Aug 2022 17:49:09: 31000000 INFO @ Tue, 02 Aug 2022 17:49:10: 28000000 INFO @ Tue, 02 Aug 2022 17:49:14: 38000000 INFO @ Tue, 02 Aug 2022 17:49:16: 32000000 INFO @ Tue, 02 Aug 2022 17:49:17: 29000000 INFO @ Tue, 02 Aug 2022 17:49:20: 39000000 INFO @ Tue, 02 Aug 2022 17:49:23: 33000000 INFO @ Tue, 02 Aug 2022 17:49:23: 30000000 INFO @ Tue, 02 Aug 2022 17:49:27: 40000000 INFO @ Tue, 02 Aug 2022 17:49:29: 34000000 INFO @ Tue, 02 Aug 2022 17:49:30: 31000000 INFO @ Tue, 02 Aug 2022 17:49:34: 41000000 INFO @ Tue, 02 Aug 2022 17:49:36: 35000000 INFO @ Tue, 02 Aug 2022 17:49:37: 32000000 INFO @ Tue, 02 Aug 2022 17:49:41: 42000000 INFO @ Tue, 02 Aug 2022 17:49:43: 36000000 INFO @ Tue, 02 Aug 2022 17:49:44: 33000000 INFO @ Tue, 02 Aug 2022 17:49:47: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:49:49: 37000000 INFO @ Tue, 02 Aug 2022 17:49:51: 34000000 INFO @ Tue, 02 Aug 2022 17:49:54: 44000000 INFO @ Tue, 02 Aug 2022 17:49:56: 38000000 INFO @ Tue, 02 Aug 2022 17:49:57: 35000000 INFO @ Tue, 02 Aug 2022 17:50:02: 45000000 INFO @ Tue, 02 Aug 2022 17:50:03: 39000000 INFO @ Tue, 02 Aug 2022 17:50:04: 36000000 INFO @ Tue, 02 Aug 2022 17:50:09: 46000000 INFO @ Tue, 02 Aug 2022 17:50:10: 40000000 INFO @ Tue, 02 Aug 2022 17:50:11: 37000000 INFO @ Tue, 02 Aug 2022 17:50:16: 47000000 INFO @ Tue, 02 Aug 2022 17:50:16: 41000000 INFO @ Tue, 02 Aug 2022 17:50:18: 38000000 INFO @ Tue, 02 Aug 2022 17:50:23: 48000000 INFO @ Tue, 02 Aug 2022 17:50:23: 42000000 INFO @ Tue, 02 Aug 2022 17:50:25: 39000000 INFO @ Tue, 02 Aug 2022 17:50:30: 49000000 INFO @ Tue, 02 Aug 2022 17:50:30: 43000000 INFO @ Tue, 02 Aug 2022 17:50:31: 40000000 INFO @ Tue, 02 Aug 2022 17:50:37: 50000000 INFO @ Tue, 02 Aug 2022 17:50:37: 44000000 INFO @ Tue, 02 Aug 2022 17:50:38: 41000000 INFO @ Tue, 02 Aug 2022 17:50:44: 51000000 INFO @ Tue, 02 Aug 2022 17:50:44: 45000000 INFO @ Tue, 02 Aug 2022 17:50:45: 42000000 INFO @ Tue, 02 Aug 2022 17:50:51: 52000000 INFO @ Tue, 02 Aug 2022 17:50:52: 46000000 INFO @ Tue, 02 Aug 2022 17:50:52: 43000000 INFO @ Tue, 02 Aug 2022 17:50:58: 53000000 INFO @ Tue, 02 Aug 2022 17:50:59: 47000000 INFO @ Tue, 02 Aug 2022 17:50:59: 44000000 INFO @ Tue, 02 Aug 2022 17:51:05: 54000000 INFO @ Tue, 02 Aug 2022 17:51:06: 48000000 INFO @ Tue, 02 Aug 2022 17:51:06: 45000000 INFO @ Tue, 02 Aug 2022 17:51:12: 55000000 INFO @ Tue, 02 Aug 2022 17:51:13: 49000000 INFO @ Tue, 02 Aug 2022 17:51:13: 46000000 INFO @ Tue, 02 Aug 2022 17:51:19: 56000000 INFO @ Tue, 02 Aug 2022 17:51:20: 47000000 INFO @ Tue, 02 Aug 2022 17:51:20: 50000000 INFO @ Tue, 02 Aug 2022 17:51:26: 57000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 17:51:27: 48000000 INFO @ Tue, 02 Aug 2022 17:51:27: 51000000 INFO @ Tue, 02 Aug 2022 17:51:33: 58000000 INFO @ Tue, 02 Aug 2022 17:51:34: 49000000 INFO @ Tue, 02 Aug 2022 17:51:35: 52000000 INFO @ Tue, 02 Aug 2022 17:51:40: 59000000 INFO @ Tue, 02 Aug 2022 17:51:41: 50000000 INFO @ Tue, 02 Aug 2022 17:51:42: 53000000 INFO @ Tue, 02 Aug 2022 17:51:47: 60000000 INFO @ Tue, 02 Aug 2022 17:51:49: 51000000 INFO @ Tue, 02 Aug 2022 17:51:49: 54000000 INFO @ Tue, 02 Aug 2022 17:51:54: 61000000 INFO @ Tue, 02 Aug 2022 17:51:55: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:51:55: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:51:55: #1 total tags in treatment: 61024265 INFO @ Tue, 02 Aug 2022 17:51:55: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:51:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:51:55: 52000000 INFO @ Tue, 02 Aug 2022 17:51:56: 55000000 INFO @ Tue, 02 Aug 2022 17:51:56: #1 tags after filtering in treatment: 61024265 INFO @ Tue, 02 Aug 2022 17:51:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:51:56: #1 finished! INFO @ Tue, 02 Aug 2022 17:51:56: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:51:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:52:00: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 17:52:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:52:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:52:02: 53000000 INFO @ Tue, 02 Aug 2022 17:52:03: 56000000 INFO @ Tue, 02 Aug 2022 17:52:09: 54000000 INFO @ Tue, 02 Aug 2022 17:52:10: 57000000 INFO @ Tue, 02 Aug 2022 17:52:16: 55000000 INFO @ Tue, 02 Aug 2022 17:52:17: 58000000 INFO @ Tue, 02 Aug 2022 17:52:23: 56000000 INFO @ Tue, 02 Aug 2022 17:52:25: 59000000 INFO @ Tue, 02 Aug 2022 17:52:30: 57000000 INFO @ Tue, 02 Aug 2022 17:52:32: 60000000 INFO @ Tue, 02 Aug 2022 17:52:37: 58000000 INFO @ Tue, 02 Aug 2022 17:52:38: 61000000 INFO @ Tue, 02 Aug 2022 17:52:39: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:52:39: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:52:39: #1 total tags in treatment: 61024265 INFO @ Tue, 02 Aug 2022 17:52:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:52:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:52:40: #1 tags after filtering in treatment: 61024265 INFO @ Tue, 02 Aug 2022 17:52:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:52:40: #1 finished! INFO @ Tue, 02 Aug 2022 17:52:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:52:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:52:43: 59000000 INFO @ Tue, 02 Aug 2022 17:52:44: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 17:52:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:52:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:52:50: 60000000 INFO @ Tue, 02 Aug 2022 17:52:56: 61000000 INFO @ Tue, 02 Aug 2022 17:52:56: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:52:56: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:52:56: #1 total tags in treatment: 61024265 INFO @ Tue, 02 Aug 2022 17:52:56: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:52:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:52:58: #1 tags after filtering in treatment: 61024265 INFO @ Tue, 02 Aug 2022 17:52:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:52:58: #1 finished! INFO @ Tue, 02 Aug 2022 17:52:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:52:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:53:01: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 17:53:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:53:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8369897/SRX8369897.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling