Job ID = 6459787 SRX = SRX826336 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T14:02:53 prefetch.2.10.7: 1) Downloading 'SRR1737836'... 2020-06-21T14:02:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T14:06:33 prefetch.2.10.7: HTTPS download succeed 2020-06-21T14:06:33 prefetch.2.10.7: 1) 'SRR1737836' was downloaded successfully Read 48706360 spots for SRR1737836/SRR1737836.sra Written 48706360 spots for SRR1737836/SRR1737836.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:23 48706360 reads; of these: 48706360 (100.00%) were unpaired; of these: 8662771 (17.79%) aligned 0 times 36367598 (74.67%) aligned exactly 1 time 3675991 (7.55%) aligned >1 times 82.21% overall alignment rate Time searching: 00:07:23 Overall time: 00:07:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4767890 / 40043589 = 0.1191 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:23:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:23:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:23:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:24:00: 1000000 INFO @ Sun, 21 Jun 2020 23:24:06: 2000000 INFO @ Sun, 21 Jun 2020 23:24:13: 3000000 INFO @ Sun, 21 Jun 2020 23:24:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:24:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:24:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:24:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:24:25: 5000000 INFO @ Sun, 21 Jun 2020 23:24:31: 1000000 INFO @ Sun, 21 Jun 2020 23:24:32: 6000000 INFO @ Sun, 21 Jun 2020 23:24:38: 2000000 INFO @ Sun, 21 Jun 2020 23:24:39: 7000000 INFO @ Sun, 21 Jun 2020 23:24:45: 3000000 INFO @ Sun, 21 Jun 2020 23:24:45: 8000000 INFO @ Sun, 21 Jun 2020 23:24:52: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:24:52: 9000000 INFO @ Sun, 21 Jun 2020 23:24:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:24:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:24:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:24:59: 5000000 INFO @ Sun, 21 Jun 2020 23:24:59: 10000000 INFO @ Sun, 21 Jun 2020 23:25:01: 1000000 INFO @ Sun, 21 Jun 2020 23:25:06: 11000000 INFO @ Sun, 21 Jun 2020 23:25:06: 6000000 INFO @ Sun, 21 Jun 2020 23:25:08: 2000000 INFO @ Sun, 21 Jun 2020 23:25:13: 12000000 INFO @ Sun, 21 Jun 2020 23:25:14: 7000000 INFO @ Sun, 21 Jun 2020 23:25:15: 3000000 INFO @ Sun, 21 Jun 2020 23:25:20: 13000000 INFO @ Sun, 21 Jun 2020 23:25:21: 8000000 INFO @ Sun, 21 Jun 2020 23:25:23: 4000000 INFO @ Sun, 21 Jun 2020 23:25:27: 14000000 INFO @ Sun, 21 Jun 2020 23:25:28: 9000000 INFO @ Sun, 21 Jun 2020 23:25:30: 5000000 INFO @ Sun, 21 Jun 2020 23:25:34: 15000000 INFO @ Sun, 21 Jun 2020 23:25:35: 10000000 INFO @ Sun, 21 Jun 2020 23:25:37: 6000000 INFO @ Sun, 21 Jun 2020 23:25:41: 16000000 INFO @ Sun, 21 Jun 2020 23:25:43: 11000000 INFO @ Sun, 21 Jun 2020 23:25:44: 7000000 INFO @ Sun, 21 Jun 2020 23:25:49: 17000000 INFO @ Sun, 21 Jun 2020 23:25:50: 12000000 INFO @ Sun, 21 Jun 2020 23:25:52: 8000000 INFO @ Sun, 21 Jun 2020 23:25:56: 18000000 INFO @ Sun, 21 Jun 2020 23:25:57: 13000000 INFO @ Sun, 21 Jun 2020 23:25:59: 9000000 INFO @ Sun, 21 Jun 2020 23:26:03: 19000000 INFO @ Sun, 21 Jun 2020 23:26:04: 14000000 INFO @ Sun, 21 Jun 2020 23:26:06: 10000000 INFO @ Sun, 21 Jun 2020 23:26:10: 20000000 INFO @ Sun, 21 Jun 2020 23:26:11: 15000000 INFO @ Sun, 21 Jun 2020 23:26:13: 11000000 INFO @ Sun, 21 Jun 2020 23:26:17: 21000000 INFO @ Sun, 21 Jun 2020 23:26:19: 16000000 INFO @ Sun, 21 Jun 2020 23:26:20: 12000000 INFO @ Sun, 21 Jun 2020 23:26:24: 22000000 INFO @ Sun, 21 Jun 2020 23:26:26: 17000000 INFO @ Sun, 21 Jun 2020 23:26:27: 13000000 INFO @ Sun, 21 Jun 2020 23:26:31: 23000000 INFO @ Sun, 21 Jun 2020 23:26:33: 18000000 INFO @ Sun, 21 Jun 2020 23:26:34: 14000000 INFO @ Sun, 21 Jun 2020 23:26:38: 24000000 INFO @ Sun, 21 Jun 2020 23:26:40: 19000000 INFO @ Sun, 21 Jun 2020 23:26:42: 15000000 INFO @ Sun, 21 Jun 2020 23:26:45: 25000000 INFO @ Sun, 21 Jun 2020 23:26:48: 20000000 INFO @ Sun, 21 Jun 2020 23:26:49: 16000000 INFO @ Sun, 21 Jun 2020 23:26:52: 26000000 INFO @ Sun, 21 Jun 2020 23:26:55: 21000000 INFO @ Sun, 21 Jun 2020 23:26:56: 17000000 INFO @ Sun, 21 Jun 2020 23:26:59: 27000000 INFO @ Sun, 21 Jun 2020 23:27:02: 22000000 INFO @ Sun, 21 Jun 2020 23:27:03: 18000000 INFO @ Sun, 21 Jun 2020 23:27:06: 28000000 INFO @ Sun, 21 Jun 2020 23:27:09: 23000000 INFO @ Sun, 21 Jun 2020 23:27:10: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 23:27:13: 29000000 INFO @ Sun, 21 Jun 2020 23:27:16: 24000000 INFO @ Sun, 21 Jun 2020 23:27:17: 20000000 INFO @ Sun, 21 Jun 2020 23:27:20: 30000000 INFO @ Sun, 21 Jun 2020 23:27:22: 25000000 INFO @ Sun, 21 Jun 2020 23:27:24: 21000000 INFO @ Sun, 21 Jun 2020 23:27:27: 31000000 INFO @ Sun, 21 Jun 2020 23:27:29: 26000000 INFO @ Sun, 21 Jun 2020 23:27:32: 22000000 INFO @ Sun, 21 Jun 2020 23:27:34: 32000000 INFO @ Sun, 21 Jun 2020 23:27:36: 27000000 INFO @ Sun, 21 Jun 2020 23:27:39: 23000000 INFO @ Sun, 21 Jun 2020 23:27:41: 33000000 INFO @ Sun, 21 Jun 2020 23:27:43: 28000000 INFO @ Sun, 21 Jun 2020 23:27:46: 24000000 INFO @ Sun, 21 Jun 2020 23:27:48: 34000000 INFO @ Sun, 21 Jun 2020 23:27:50: 29000000 INFO @ Sun, 21 Jun 2020 23:27:54: 25000000 INFO @ Sun, 21 Jun 2020 23:27:55: 35000000 INFO @ Sun, 21 Jun 2020 23:27:57: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 23:27:57: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 23:27:57: #1 total tags in treatment: 35275699 INFO @ Sun, 21 Jun 2020 23:27:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:27:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:27:57: 30000000 INFO @ Sun, 21 Jun 2020 23:27:58: #1 tags after filtering in treatment: 35275628 INFO @ Sun, 21 Jun 2020 23:27:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:27:58: #1 finished! INFO @ Sun, 21 Jun 2020 23:27:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:27:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:28:00: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 23:28:00: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:28:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:28:01: 26000000 INFO @ Sun, 21 Jun 2020 23:28:04: 31000000 INFO @ Sun, 21 Jun 2020 23:28:08: 27000000 INFO @ Sun, 21 Jun 2020 23:28:11: 32000000 INFO @ Sun, 21 Jun 2020 23:28:15: 28000000 INFO @ Sun, 21 Jun 2020 23:28:18: 33000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 23:28:23: 29000000 INFO @ Sun, 21 Jun 2020 23:28:25: 34000000 INFO @ Sun, 21 Jun 2020 23:28:30: 30000000 INFO @ Sun, 21 Jun 2020 23:28:32: 35000000 INFO @ Sun, 21 Jun 2020 23:28:35: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 23:28:35: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 23:28:35: #1 total tags in treatment: 35275699 INFO @ Sun, 21 Jun 2020 23:28:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:28:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:28:35: #1 tags after filtering in treatment: 35275628 INFO @ Sun, 21 Jun 2020 23:28:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:28:35: #1 finished! INFO @ Sun, 21 Jun 2020 23:28:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:28:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:28:37: 31000000 INFO @ Sun, 21 Jun 2020 23:28:37: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 23:28:37: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:28:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:28:44: 32000000 INFO @ Sun, 21 Jun 2020 23:28:50: 33000000 INFO @ Sun, 21 Jun 2020 23:28:57: 34000000 INFO @ Sun, 21 Jun 2020 23:29:03: 35000000 INFO @ Sun, 21 Jun 2020 23:29:06: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 23:29:06: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 23:29:06: #1 total tags in treatment: 35275699 INFO @ Sun, 21 Jun 2020 23:29:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:29:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:29:06: #1 tags after filtering in treatment: 35275628 INFO @ Sun, 21 Jun 2020 23:29:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:29:06: #1 finished! INFO @ Sun, 21 Jun 2020 23:29:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:29:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:29:09: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 23:29:09: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:29:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX826336/SRX826336.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling