Job ID = 14171018 SRX = SRX8174958 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:51 68573462 reads; of these: 68573462 (100.00%) were unpaired; of these: 5667397 (8.26%) aligned 0 times 54607053 (79.63%) aligned exactly 1 time 8299012 (12.10%) aligned >1 times 91.74% overall alignment rate Time searching: 00:22:51 Overall time: 00:22:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13509632 / 62906065 = 0.2148 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:48:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:48:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:48:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:48:09: 1000000 INFO @ Sat, 11 Dec 2021 09:48:15: 2000000 INFO @ Sat, 11 Dec 2021 09:48:21: 3000000 INFO @ Sat, 11 Dec 2021 09:48:27: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:48:33: 5000000 INFO @ Sat, 11 Dec 2021 09:48:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:48:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:48:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:48:41: 6000000 INFO @ Sat, 11 Dec 2021 09:48:42: 1000000 INFO @ Sat, 11 Dec 2021 09:48:48: 7000000 INFO @ Sat, 11 Dec 2021 09:48:49: 2000000 INFO @ Sat, 11 Dec 2021 09:48:56: 8000000 INFO @ Sat, 11 Dec 2021 09:48:56: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:49:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:49:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:49:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:49:03: 9000000 INFO @ Sat, 11 Dec 2021 09:49:05: 4000000 INFO @ Sat, 11 Dec 2021 09:49:11: 1000000 INFO @ Sat, 11 Dec 2021 09:49:11: 10000000 INFO @ Sat, 11 Dec 2021 09:49:13: 5000000 INFO @ Sat, 11 Dec 2021 09:49:19: 2000000 INFO @ Sat, 11 Dec 2021 09:49:19: 11000000 INFO @ Sat, 11 Dec 2021 09:49:21: 6000000 INFO @ Sat, 11 Dec 2021 09:49:26: 3000000 INFO @ Sat, 11 Dec 2021 09:49:26: 12000000 INFO @ Sat, 11 Dec 2021 09:49:28: 7000000 INFO @ Sat, 11 Dec 2021 09:49:34: 13000000 INFO @ Sat, 11 Dec 2021 09:49:34: 4000000 INFO @ Sat, 11 Dec 2021 09:49:35: 8000000 INFO @ Sat, 11 Dec 2021 09:49:41: 14000000 INFO @ Sat, 11 Dec 2021 09:49:41: 5000000 INFO @ Sat, 11 Dec 2021 09:49:43: 9000000 INFO @ Sat, 11 Dec 2021 09:49:49: 15000000 INFO @ Sat, 11 Dec 2021 09:49:49: 6000000 INFO @ Sat, 11 Dec 2021 09:49:50: 10000000 INFO @ Sat, 11 Dec 2021 09:49:56: 7000000 INFO @ Sat, 11 Dec 2021 09:49:57: 16000000 INFO @ Sat, 11 Dec 2021 09:49:58: 11000000 INFO @ Sat, 11 Dec 2021 09:50:04: 8000000 INFO @ Sat, 11 Dec 2021 09:50:04: 17000000 INFO @ Sat, 11 Dec 2021 09:50:05: 12000000 INFO @ Sat, 11 Dec 2021 09:50:11: 9000000 INFO @ Sat, 11 Dec 2021 09:50:11: 18000000 INFO @ Sat, 11 Dec 2021 09:50:12: 13000000 INFO @ Sat, 11 Dec 2021 09:50:19: 19000000 INFO @ Sat, 11 Dec 2021 09:50:19: 10000000 INFO @ Sat, 11 Dec 2021 09:50:20: 14000000 INFO @ Sat, 11 Dec 2021 09:50:26: 20000000 INFO @ Sat, 11 Dec 2021 09:50:26: 11000000 INFO @ Sat, 11 Dec 2021 09:50:27: 15000000 INFO @ Sat, 11 Dec 2021 09:50:34: 21000000 INFO @ Sat, 11 Dec 2021 09:50:34: 12000000 INFO @ Sat, 11 Dec 2021 09:50:34: 16000000 INFO @ Sat, 11 Dec 2021 09:50:41: 22000000 INFO @ Sat, 11 Dec 2021 09:50:41: 13000000 INFO @ Sat, 11 Dec 2021 09:50:42: 17000000 INFO @ Sat, 11 Dec 2021 09:50:48: 23000000 INFO @ Sat, 11 Dec 2021 09:50:49: 14000000 INFO @ Sat, 11 Dec 2021 09:50:49: 18000000 INFO @ Sat, 11 Dec 2021 09:50:56: 24000000 INFO @ Sat, 11 Dec 2021 09:50:56: 15000000 INFO @ Sat, 11 Dec 2021 09:50:56: 19000000 INFO @ Sat, 11 Dec 2021 09:51:03: 25000000 INFO @ Sat, 11 Dec 2021 09:51:03: 16000000 INFO @ Sat, 11 Dec 2021 09:51:04: 20000000 INFO @ Sat, 11 Dec 2021 09:51:10: 26000000 INFO @ Sat, 11 Dec 2021 09:51:11: 17000000 INFO @ Sat, 11 Dec 2021 09:51:11: 21000000 INFO @ Sat, 11 Dec 2021 09:51:17: 27000000 INFO @ Sat, 11 Dec 2021 09:51:18: 18000000 INFO @ Sat, 11 Dec 2021 09:51:18: 22000000 INFO @ Sat, 11 Dec 2021 09:51:24: 28000000 INFO @ Sat, 11 Dec 2021 09:51:25: 19000000 INFO @ Sat, 11 Dec 2021 09:51:25: 23000000 INFO @ Sat, 11 Dec 2021 09:51:32: 29000000 INFO @ Sat, 11 Dec 2021 09:51:32: 20000000 INFO @ Sat, 11 Dec 2021 09:51:32: 24000000 INFO @ Sat, 11 Dec 2021 09:51:39: 30000000 INFO @ Sat, 11 Dec 2021 09:51:40: 25000000 INFO @ Sat, 11 Dec 2021 09:51:40: 21000000 INFO @ Sat, 11 Dec 2021 09:51:46: 31000000 INFO @ Sat, 11 Dec 2021 09:51:47: 26000000 INFO @ Sat, 11 Dec 2021 09:51:47: 22000000 INFO @ Sat, 11 Dec 2021 09:51:53: 32000000 INFO @ Sat, 11 Dec 2021 09:51:54: 27000000 INFO @ Sat, 11 Dec 2021 09:51:54: 23000000 INFO @ Sat, 11 Dec 2021 09:52:00: 33000000 INFO @ Sat, 11 Dec 2021 09:52:01: 28000000 INFO @ Sat, 11 Dec 2021 09:52:02: 24000000 INFO @ Sat, 11 Dec 2021 09:52:08: 34000000 INFO @ Sat, 11 Dec 2021 09:52:09: 29000000 INFO @ Sat, 11 Dec 2021 09:52:09: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:52:15: 35000000 INFO @ Sat, 11 Dec 2021 09:52:17: 30000000 INFO @ Sat, 11 Dec 2021 09:52:17: 26000000 INFO @ Sat, 11 Dec 2021 09:52:23: 36000000 INFO @ Sat, 11 Dec 2021 09:52:24: 31000000 INFO @ Sat, 11 Dec 2021 09:52:25: 27000000 INFO @ Sat, 11 Dec 2021 09:52:31: 37000000 INFO @ Sat, 11 Dec 2021 09:52:32: 32000000 INFO @ Sat, 11 Dec 2021 09:52:32: 28000000 INFO @ Sat, 11 Dec 2021 09:52:38: 38000000 INFO @ Sat, 11 Dec 2021 09:52:40: 33000000 INFO @ Sat, 11 Dec 2021 09:52:40: 29000000 INFO @ Sat, 11 Dec 2021 09:52:46: 39000000 INFO @ Sat, 11 Dec 2021 09:52:48: 30000000 INFO @ Sat, 11 Dec 2021 09:52:48: 34000000 INFO @ Sat, 11 Dec 2021 09:52:54: 40000000 INFO @ Sat, 11 Dec 2021 09:52:56: 31000000 INFO @ Sat, 11 Dec 2021 09:52:56: 35000000 INFO @ Sat, 11 Dec 2021 09:53:01: 41000000 INFO @ Sat, 11 Dec 2021 09:53:03: 32000000 INFO @ Sat, 11 Dec 2021 09:53:04: 36000000 INFO @ Sat, 11 Dec 2021 09:53:09: 42000000 INFO @ Sat, 11 Dec 2021 09:53:11: 33000000 INFO @ Sat, 11 Dec 2021 09:53:12: 37000000 INFO @ Sat, 11 Dec 2021 09:53:17: 43000000 INFO @ Sat, 11 Dec 2021 09:53:19: 34000000 INFO @ Sat, 11 Dec 2021 09:53:19: 38000000 INFO @ Sat, 11 Dec 2021 09:53:24: 44000000 INFO @ Sat, 11 Dec 2021 09:53:26: 35000000 INFO @ Sat, 11 Dec 2021 09:53:27: 39000000 INFO @ Sat, 11 Dec 2021 09:53:32: 45000000 INFO @ Sat, 11 Dec 2021 09:53:34: 36000000 INFO @ Sat, 11 Dec 2021 09:53:34: 40000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 09:53:39: 46000000 INFO @ Sat, 11 Dec 2021 09:53:41: 37000000 INFO @ Sat, 11 Dec 2021 09:53:42: 41000000 INFO @ Sat, 11 Dec 2021 09:53:47: 47000000 INFO @ Sat, 11 Dec 2021 09:53:49: 38000000 INFO @ Sat, 11 Dec 2021 09:53:50: 42000000 INFO @ Sat, 11 Dec 2021 09:53:54: 48000000 INFO @ Sat, 11 Dec 2021 09:53:56: 39000000 INFO @ Sat, 11 Dec 2021 09:53:57: 43000000 INFO @ Sat, 11 Dec 2021 09:54:02: 49000000 INFO @ Sat, 11 Dec 2021 09:54:03: 40000000 INFO @ Sat, 11 Dec 2021 09:54:04: 44000000 INFO @ Sat, 11 Dec 2021 09:54:05: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 09:54:05: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 09:54:05: #1 total tags in treatment: 49396433 INFO @ Sat, 11 Dec 2021 09:54:05: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:54:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:54:06: #1 tags after filtering in treatment: 49396415 INFO @ Sat, 11 Dec 2021 09:54:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:54:06: #1 finished! INFO @ Sat, 11 Dec 2021 09:54:06: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:54:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:54:09: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 09:54:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:54:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:54:11: 41000000 INFO @ Sat, 11 Dec 2021 09:54:11: 45000000 INFO @ Sat, 11 Dec 2021 09:54:18: 42000000 INFO @ Sat, 11 Dec 2021 09:54:18: 46000000 INFO @ Sat, 11 Dec 2021 09:54:25: 43000000 INFO @ Sat, 11 Dec 2021 09:54:26: 47000000 INFO @ Sat, 11 Dec 2021 09:54:32: 44000000 INFO @ Sat, 11 Dec 2021 09:54:33: 48000000 INFO @ Sat, 11 Dec 2021 09:54:40: 45000000 INFO @ Sat, 11 Dec 2021 09:54:40: 49000000 INFO @ Sat, 11 Dec 2021 09:54:43: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 09:54:43: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 09:54:43: #1 total tags in treatment: 49396433 INFO @ Sat, 11 Dec 2021 09:54:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:54:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:54:44: #1 tags after filtering in treatment: 49396415 INFO @ Sat, 11 Dec 2021 09:54:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:54:44: #1 finished! INFO @ Sat, 11 Dec 2021 09:54:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:54:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:54:46: 46000000 INFO @ Sat, 11 Dec 2021 09:54:47: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 09:54:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:54:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:54:53: 47000000 INFO @ Sat, 11 Dec 2021 09:54:58: 48000000 INFO @ Sat, 11 Dec 2021 09:55:05: 49000000 INFO @ Sat, 11 Dec 2021 09:55:07: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 09:55:07: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 09:55:07: #1 total tags in treatment: 49396433 INFO @ Sat, 11 Dec 2021 09:55:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:55:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:55:08: #1 tags after filtering in treatment: 49396415 INFO @ Sat, 11 Dec 2021 09:55:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:55:08: #1 finished! INFO @ Sat, 11 Dec 2021 09:55:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:55:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:55:11: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 09:55:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:55:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174958/SRX8174958.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling