Job ID = 14171032 SRX = SRX8174954 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:39 71023950 reads; of these: 71023950 (100.00%) were unpaired; of these: 9831511 (13.84%) aligned 0 times 53276298 (75.01%) aligned exactly 1 time 7916141 (11.15%) aligned >1 times 86.16% overall alignment rate Time searching: 00:21:39 Overall time: 00:21:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 14335757 / 61192439 = 0.2343 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:47:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:47:41: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:47:41: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:47:47: 1000000 INFO @ Sat, 11 Dec 2021 09:47:53: 2000000 INFO @ Sat, 11 Dec 2021 09:47:59: 3000000 INFO @ Sat, 11 Dec 2021 09:48:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:48:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:48:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:48:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:48:12: 5000000 INFO @ Sat, 11 Dec 2021 09:48:20: 6000000 INFO @ Sat, 11 Dec 2021 09:48:20: 1000000 INFO @ Sat, 11 Dec 2021 09:48:28: 7000000 INFO @ Sat, 11 Dec 2021 09:48:29: 2000000 INFO @ Sat, 11 Dec 2021 09:48:36: 8000000 INFO @ Sat, 11 Dec 2021 09:48:38: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:48:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:48:41: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:48:41: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:48:44: 9000000 INFO @ Sat, 11 Dec 2021 09:48:47: 4000000 INFO @ Sat, 11 Dec 2021 09:48:50: 1000000 INFO @ Sat, 11 Dec 2021 09:48:52: 10000000 INFO @ Sat, 11 Dec 2021 09:48:56: 5000000 INFO @ Sat, 11 Dec 2021 09:48:59: 2000000 INFO @ Sat, 11 Dec 2021 09:49:00: 11000000 INFO @ Sat, 11 Dec 2021 09:49:05: 6000000 INFO @ Sat, 11 Dec 2021 09:49:08: 12000000 INFO @ Sat, 11 Dec 2021 09:49:08: 3000000 INFO @ Sat, 11 Dec 2021 09:49:14: 7000000 INFO @ Sat, 11 Dec 2021 09:49:16: 13000000 INFO @ Sat, 11 Dec 2021 09:49:18: 4000000 INFO @ Sat, 11 Dec 2021 09:49:23: 8000000 INFO @ Sat, 11 Dec 2021 09:49:24: 14000000 INFO @ Sat, 11 Dec 2021 09:49:27: 5000000 INFO @ Sat, 11 Dec 2021 09:49:33: 9000000 INFO @ Sat, 11 Dec 2021 09:49:33: 15000000 INFO @ Sat, 11 Dec 2021 09:49:36: 6000000 INFO @ Sat, 11 Dec 2021 09:49:41: 16000000 INFO @ Sat, 11 Dec 2021 09:49:42: 10000000 INFO @ Sat, 11 Dec 2021 09:49:45: 7000000 INFO @ Sat, 11 Dec 2021 09:49:49: 17000000 INFO @ Sat, 11 Dec 2021 09:49:51: 11000000 INFO @ Sat, 11 Dec 2021 09:49:54: 8000000 INFO @ Sat, 11 Dec 2021 09:49:57: 18000000 INFO @ Sat, 11 Dec 2021 09:50:00: 12000000 INFO @ Sat, 11 Dec 2021 09:50:03: 9000000 INFO @ Sat, 11 Dec 2021 09:50:05: 19000000 INFO @ Sat, 11 Dec 2021 09:50:08: 13000000 INFO @ Sat, 11 Dec 2021 09:50:12: 10000000 INFO @ Sat, 11 Dec 2021 09:50:13: 20000000 INFO @ Sat, 11 Dec 2021 09:50:17: 14000000 INFO @ Sat, 11 Dec 2021 09:50:21: 11000000 INFO @ Sat, 11 Dec 2021 09:50:22: 21000000 INFO @ Sat, 11 Dec 2021 09:50:26: 15000000 INFO @ Sat, 11 Dec 2021 09:50:30: 22000000 INFO @ Sat, 11 Dec 2021 09:50:30: 12000000 INFO @ Sat, 11 Dec 2021 09:50:36: 16000000 INFO @ Sat, 11 Dec 2021 09:50:38: 23000000 INFO @ Sat, 11 Dec 2021 09:50:39: 13000000 INFO @ Sat, 11 Dec 2021 09:50:45: 17000000 INFO @ Sat, 11 Dec 2021 09:50:46: 24000000 INFO @ Sat, 11 Dec 2021 09:50:48: 14000000 INFO @ Sat, 11 Dec 2021 09:50:54: 25000000 INFO @ Sat, 11 Dec 2021 09:50:54: 18000000 INFO @ Sat, 11 Dec 2021 09:50:57: 15000000 INFO @ Sat, 11 Dec 2021 09:51:02: 26000000 INFO @ Sat, 11 Dec 2021 09:51:03: 19000000 INFO @ Sat, 11 Dec 2021 09:51:06: 16000000 INFO @ Sat, 11 Dec 2021 09:51:10: 27000000 INFO @ Sat, 11 Dec 2021 09:51:13: 20000000 INFO @ Sat, 11 Dec 2021 09:51:15: 17000000 INFO @ Sat, 11 Dec 2021 09:51:18: 28000000 INFO @ Sat, 11 Dec 2021 09:51:22: 21000000 INFO @ Sat, 11 Dec 2021 09:51:24: 18000000 INFO @ Sat, 11 Dec 2021 09:51:26: 29000000 INFO @ Sat, 11 Dec 2021 09:51:31: 22000000 INFO @ Sat, 11 Dec 2021 09:51:33: 19000000 INFO @ Sat, 11 Dec 2021 09:51:34: 30000000 INFO @ Sat, 11 Dec 2021 09:51:40: 23000000 INFO @ Sat, 11 Dec 2021 09:51:42: 20000000 INFO @ Sat, 11 Dec 2021 09:51:42: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:51:49: 24000000 INFO @ Sat, 11 Dec 2021 09:51:50: 32000000 INFO @ Sat, 11 Dec 2021 09:51:51: 21000000 INFO @ Sat, 11 Dec 2021 09:51:58: 33000000 INFO @ Sat, 11 Dec 2021 09:51:58: 25000000 INFO @ Sat, 11 Dec 2021 09:52:00: 22000000 INFO @ Sat, 11 Dec 2021 09:52:06: 34000000 INFO @ Sat, 11 Dec 2021 09:52:07: 26000000 INFO @ Sat, 11 Dec 2021 09:52:09: 23000000 INFO @ Sat, 11 Dec 2021 09:52:14: 35000000 INFO @ Sat, 11 Dec 2021 09:52:16: 27000000 INFO @ Sat, 11 Dec 2021 09:52:18: 24000000 INFO @ Sat, 11 Dec 2021 09:52:22: 36000000 INFO @ Sat, 11 Dec 2021 09:52:25: 28000000 INFO @ Sat, 11 Dec 2021 09:52:26: 25000000 INFO @ Sat, 11 Dec 2021 09:52:30: 37000000 INFO @ Sat, 11 Dec 2021 09:52:34: 29000000 INFO @ Sat, 11 Dec 2021 09:52:35: 26000000 INFO @ Sat, 11 Dec 2021 09:52:38: 38000000 INFO @ Sat, 11 Dec 2021 09:52:43: 30000000 INFO @ Sat, 11 Dec 2021 09:52:44: 27000000 INFO @ Sat, 11 Dec 2021 09:52:47: 39000000 INFO @ Sat, 11 Dec 2021 09:52:52: 31000000 INFO @ Sat, 11 Dec 2021 09:52:53: 28000000 INFO @ Sat, 11 Dec 2021 09:52:55: 40000000 INFO @ Sat, 11 Dec 2021 09:53:01: 32000000 INFO @ Sat, 11 Dec 2021 09:53:02: 29000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 09:53:03: 41000000 INFO @ Sat, 11 Dec 2021 09:53:10: 30000000 INFO @ Sat, 11 Dec 2021 09:53:10: 33000000 INFO @ Sat, 11 Dec 2021 09:53:11: 42000000 INFO @ Sat, 11 Dec 2021 09:53:19: 31000000 INFO @ Sat, 11 Dec 2021 09:53:19: 43000000 INFO @ Sat, 11 Dec 2021 09:53:20: 34000000 INFO @ Sat, 11 Dec 2021 09:53:27: 44000000 INFO @ Sat, 11 Dec 2021 09:53:28: 32000000 INFO @ Sat, 11 Dec 2021 09:53:29: 35000000 INFO @ Sat, 11 Dec 2021 09:53:35: 45000000 INFO @ Sat, 11 Dec 2021 09:53:37: 33000000 INFO @ Sat, 11 Dec 2021 09:53:38: 36000000 INFO @ Sat, 11 Dec 2021 09:53:44: 46000000 INFO @ Sat, 11 Dec 2021 09:53:46: 34000000 INFO @ Sat, 11 Dec 2021 09:53:47: 37000000 INFO @ Sat, 11 Dec 2021 09:53:51: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 09:53:51: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 09:53:51: #1 total tags in treatment: 46856682 INFO @ Sat, 11 Dec 2021 09:53:51: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:53:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:53:52: #1 tags after filtering in treatment: 46856658 INFO @ Sat, 11 Dec 2021 09:53:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:53:52: #1 finished! INFO @ Sat, 11 Dec 2021 09:53:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:53:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:53:55: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 09:53:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:53:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:53:55: 35000000 INFO @ Sat, 11 Dec 2021 09:53:56: 38000000 INFO @ Sat, 11 Dec 2021 09:54:04: 36000000 INFO @ Sat, 11 Dec 2021 09:54:05: 39000000 INFO @ Sat, 11 Dec 2021 09:54:13: 37000000 INFO @ Sat, 11 Dec 2021 09:54:14: 40000000 INFO @ Sat, 11 Dec 2021 09:54:21: 38000000 INFO @ Sat, 11 Dec 2021 09:54:23: 41000000 INFO @ Sat, 11 Dec 2021 09:54:30: 39000000 INFO @ Sat, 11 Dec 2021 09:54:32: 42000000 INFO @ Sat, 11 Dec 2021 09:54:39: 40000000 INFO @ Sat, 11 Dec 2021 09:54:41: 43000000 INFO @ Sat, 11 Dec 2021 09:54:48: 41000000 INFO @ Sat, 11 Dec 2021 09:54:50: 44000000 INFO @ Sat, 11 Dec 2021 09:54:57: 42000000 INFO @ Sat, 11 Dec 2021 09:54:59: 45000000 INFO @ Sat, 11 Dec 2021 09:55:06: 43000000 INFO @ Sat, 11 Dec 2021 09:55:08: 46000000 INFO @ Sat, 11 Dec 2021 09:55:14: 44000000 INFO @ Sat, 11 Dec 2021 09:55:16: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 09:55:16: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 09:55:16: #1 total tags in treatment: 46856682 INFO @ Sat, 11 Dec 2021 09:55:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:55:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:55:17: #1 tags after filtering in treatment: 46856658 INFO @ Sat, 11 Dec 2021 09:55:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:55:17: #1 finished! INFO @ Sat, 11 Dec 2021 09:55:17: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:55:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:55:20: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 09:55:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:55:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:55:23: 45000000 INFO @ Sat, 11 Dec 2021 09:55:30: 46000000 INFO @ Sat, 11 Dec 2021 09:55:37: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 09:55:37: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 09:55:37: #1 total tags in treatment: 46856682 INFO @ Sat, 11 Dec 2021 09:55:37: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:55:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:55:38: #1 tags after filtering in treatment: 46856658 INFO @ Sat, 11 Dec 2021 09:55:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:55:38: #1 finished! INFO @ Sat, 11 Dec 2021 09:55:38: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:55:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:55:41: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 09:55:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:55:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX8174954/SRX8174954.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling